RchiOBHm_Chr5g0024351

Somatic embryogenesis receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001737
Physical Location & Seq
Reverse (-)
18536056 .. 18536310
255 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30410

Sequence Viewer

Length: 255 bp
ATGAGGACGACCTGCTGGATACTATTGACTCTAACCCTAGGCGCAAGTCTCATTCCTGGAGCACACCTTCTTTCAGAGACAGAAGCTTTGAATGCGTTTAAGAAGAACTTAAGTGATCCGCAAAACGTGCTCAGCACTTGGAATGCCAATCAAGCCGATCCATGCAGTTGGTCACACATCGTCTGCAATCAAGACAAAAAAGTCACTGAAGTGTTCTTAATTAACAGCCAGCTTTCTGGACATCTGGTACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_4 LRR_8

Protein Analysis

84

Amino Acids

9.25

Weight (kDa)

6.23

Isoelectric Point (pI)

22.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 63 1.6e-13 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 20
Acc65I GGTACC 1 cut(s) 247
AccB1I GGYRCC 1 cut(s) 247
AciI CCGC 1 cut(s) 119
AclWI GGATC 2 cut(s) 110, 152
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 1 cut(s) 249
AflII CTTAAG 1 cut(s) 109
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 55
AleI CACNNNNGTG 1 cut(s) 209
AluBI AGCT 2 cut(s) 86, 232
AluI AGCT 2 cut(s) 86, 232
Alw21I GWGCWC 2 cut(s) 64, 132
Alw26I GTCTC 2 cut(s) 53, 71
AlwI GGATC 2 cut(s) 110, 152
ArsI GACNNNNNNTTYG 2 cut(s) 70, 102
Asp718I GGTACC 1 cut(s) 247
AspA2I CCTAGG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 44
AvrII CCTAGG 1 cut(s) 37
BanI GGYRCC 1 cut(s) 247
Bbv12I GWGCWC 2 cut(s) 64, 132
BciT130I CCWGG 1 cut(s) 57
BciVI GTATCC 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 53, 71
BfaI CTAG 1 cut(s) 38
BfrI CTTAAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 20
BfuI GTATCC 1 cut(s) 12
BlnI CCTAGG 1 cut(s) 37
BlpI GCTNAGC 1 cut(s) 131
Bme1390I CCNGG 1 cut(s) 57
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 145
BshNI GGYRCC 1 cut(s) 247
BsiHKAI GWGCWC 2 cut(s) 64, 132
BsmAI GTCTC 2 cut(s) 53, 71
BsmI GAATGC 2 cut(s) 97, 148
Bsp1286I GDGCHC 2 cut(s) 64, 132
Bsp143I GATC 2 cut(s) 115, 157
Bsp1720I GCTNAGC 1 cut(s) 131
BspACI CCGC 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 249
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 110, 152
BspT107I GGYRCC 1 cut(s) 247
BspTI CTTAAG 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 2 cut(s) 115, 157
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 2 cut(s) 118, 160
BstMAI GTCTC 2 cut(s) 53, 71
BstMBI GATC 2 cut(s) 115, 157
BstMWI GCNNNNNNNGC 3 cut(s) 92, 127, 152
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 55
BstXI CCANNNNNNTGG 2 cut(s) 168, 236
BsuI GTATCC 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 204
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 248
CviAII CATG 1 cut(s) 162
CviJI RGCY 4 cut(s) 86, 155, 228, 232
CviKI_1 RGCY 4 cut(s) 86, 155, 228, 232
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 1 cut(s) 131
DpnI GATC 2 cut(s) 117, 159
DpnII GATC 2 cut(s) 115, 157
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 1 cut(s) 165
FaiI YATR 1 cut(s) 163
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 1 cut(s) 161
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 43
GsuI CTGGAG 1 cut(s) 78
HhaI GCGC 1 cut(s) 44
Hin1II CATG 1 cut(s) 165
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 2 cut(s) 191, 237
HpyAV CCTTC 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 165, 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 127, 152
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 1 cut(s) 165
HspAI GCGC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 251
Kzo9I GATC 2 cut(s) 115, 157
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 6 cut(s) 25, 42, 69, 222, 230, 242
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 171, 202
MalI GATC 2 cut(s) 117, 159
MboI GATC 2 cut(s) 115, 157
MboII GAAGA 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 64, 132
MluCI AATT 1 cut(s) 219
MlyI GAGTC 1 cut(s) 22
MseI TTAA 4 cut(s) 99, 110, 218, 222
MslI CAYNNNNRTG 1 cut(s) 209
MspCI CTTAAG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 57
Mva1269I GAATGC 2 cut(s) 97, 148
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 92, 127, 152
NdeII GATC 2 cut(s) 115, 157
NlaIII CATG 1 cut(s) 165
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 2 cut(s) 171, 202
OliI CACNNNNGTG 1 cut(s) 209
PacI TTAATTAA 1 cut(s) 222
PctI GAATGC 2 cut(s) 97, 148
PfoI TCCNGGA 1 cut(s) 55
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 249
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 4 cut(s) 99, 110, 218, 222
Sau3AI GATC 2 cut(s) 115, 157
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 2 cut(s) 64, 132
SetI ASST 5 cut(s) 14, 69, 88, 129, 234
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 1 cut(s) 219
SsiI CCGC 1 cut(s) 119
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 1 cut(s) 37
TaiI ACGT 1 cut(s) 129
TasI AATT 1 cut(s) 219
Tru1I TTAA 4 cut(s) 99, 110, 218, 222
Tru9I TTAA 4 cut(s) 99, 110, 218, 222
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 2 cut(s) 171, 202
Tsp45I GTSAC 2 cut(s) 171, 202
TspRI CASTG 1 cut(s) 211
Vha464I CTTAAG 1 cut(s) 109
XmaJI CCTAGG 1 cut(s) 37
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.