FvH4_3g35690

carbonate dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000548
Physical Location & Seq
Reverse (-)
30623758 .. 30626725
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35690.t1

Sequence Viewer

Length: 756 bp
ATGACTAGCTTGGTATCTGACCTTCTGACCAGTACTGAAGATCTCCTCAAGACTACTGATGTGACCAGTACCGAGGAGGAAAGCCAGGTCGATGTGGATGCTACCGAAACTGAGAACCAGAAATTTAATCCAGTTAAAACAATTATACACGGGTTCAAGCACTTCAAGACCAACTATTACGACAAAAATCCAGACCTGTTCGCTGAGCTTGCCCTGGGACAAAGCCCCAAGTTTTTGGTATTTGCGTGTTCGGATTCCCGAGTGAGTCCCTCGCATGTTCTTCATTGCCAACCTGGAGAAGCCTTTCTGGTTCGCAACATAGCTAACATGGTTCCTGCTTACGACCAGGTGAAGTACACAGGAACTGGAGCAACCATTGAGTATGCTGTTGAAGAACTCGGGGTAGAAAACATTTTGGTGGTTGGACACAGTACTTGCGGGGGAATAAAGAGGCTAATGTCTTATCCTGAGGATGGTTCTGATCCATTGGTCTTTATAGACGATTGGGTTAAAATGGCTGAAACTGTCAAGGCAAAGGTTATTGCCGAAGCTGGCGATGCTGATTTCTCGGAACAATGTGAACGTTGTGCAAGGGAAGCAGTAAATTTCTCATTGGCAAATCTACTTACCTATCCCTTCGTCCAAGAGGCATACACGGAGAAGAAACTAGCACTTCGGGGTGCTTACTATGACTTTATCAATGGAAGTTTTGAGCTCTGGGAGCATGGTTCCGACGATGTCCTTACAGTACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

252

Amino Acids

27.86

Weight (kDa)

4.55

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 79 - 236 4.8e-43 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 438
AclI AACGTT 1 cut(s) 583
AclWI GGATC 1 cut(s) 476
AcsI RAATTY 2 cut(s) 122, 604
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 5 cut(s) 34, 70, 356, 433, 750
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 3 cut(s) 157, 166, 392
AjnI CCWGG 4 cut(s) 84, 213, 292, 345
AluBI AGCT 5 cut(s) 9, 208, 323, 551, 715
AluI AGCT 5 cut(s) 9, 208, 323, 551, 715
Alw21I GWGCWC 1 cut(s) 717
AlwI GGATC 1 cut(s) 476
AlwNI CAGNNNCTG 1 cut(s) 365
Ama87I CYCGRG 2 cut(s) 258, 398
ApoI RAATTY 2 cut(s) 122, 604
Asp700I GAANNNNTTC 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 361
AvaI CYCGRG 2 cut(s) 258, 398
AxyI CCTNAGG 1 cut(s) 468
BanII GRGCYC 1 cut(s) 717
BarI GAAGNNNNNNTAC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 717
BccI CCATC 1 cut(s) 467
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 4 cut(s) 86, 215, 294, 347
BfaI CTAG 2 cut(s) 6, 668
BglII AGATCT 1 cut(s) 40
BlpI GCTNAGC 1 cut(s) 204
BmcAI AGTACT 2 cut(s) 34, 433
Bme1390I CCNGG 4 cut(s) 86, 215, 294, 347
BmeT110I CYCGRG 2 cut(s) 258, 398
BmiI GGNNCC 2 cut(s) 333, 730
BmrFI CCNGG 4 cut(s) 86, 215, 294, 347
BmsI GCATC 2 cut(s) 88, 547
BpmI CTGGAG 2 cut(s) 315, 387
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 72, 213, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse1I ACTGG 4 cut(s) 30, 66, 131, 370
Bse21I CCTNAGG 1 cut(s) 468
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 4 cut(s) 86, 215, 294, 347
BseDI CCNNGG 3 cut(s) 72, 213, 214
BseGI GGATG 2 cut(s) 103, 478
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 3 cut(s) 102, 195, 459
BseNI ACTGG 4 cut(s) 30, 66, 131, 370
BseRI GAGGAG 2 cut(s) 35, 89
BsiHKAI GWGCWC 1 cut(s) 717
BsiHKCI CYCGRG 2 cut(s) 258, 398
BslFI GGGAC 2 cut(s) 231, 252
BslI CCNNNNNNNGG 1 cut(s) 473
BsmFI GGGAC 2 cut(s) 231, 252
BsoBI CYCGRG 2 cut(s) 258, 398
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 2 cut(s) 40, 481
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 1 cut(s) 438
BspCNI CTCAG 3 cut(s) 103, 196, 460
BspLI GGNNCC 2 cut(s) 333, 730
BspPI GGATC 1 cut(s) 476
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 4 cut(s) 30, 66, 131, 370
BssECI CCNNGG 3 cut(s) 72, 213, 214
BssMI GATC 2 cut(s) 40, 481
Bst2UI CCWGG 4 cut(s) 86, 215, 294, 347
Bst4CI ACNGT 3 cut(s) 431, 526, 748
BstC8I GCNNGC 2 cut(s) 210, 553
BstDEI CTNAG 4 cut(s) 111, 204, 468, 753
BstF5I GGATG 2 cut(s) 103, 478
BstKTI GATC 2 cut(s) 43, 484
BstMBI GATC 2 cut(s) 40, 481
BstMWI GCNNNNNNNGC 4 cut(s) 209, 557, 596, 721
BstNI CCWGG 4 cut(s) 86, 215, 294, 347
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 4 cut(s) 84, 213, 292, 345
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 468
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 2 cut(s) 103, 478
Cac8I GCNNGC 2 cut(s) 210, 553
CaiI CAGNNNCTG 1 cut(s) 365
CsiI ACCWGGT 1 cut(s) 345
Csp6I GTAC 5 cut(s) 33, 69, 355, 432, 749
CviAII CATG 3 cut(s) 275, 328, 725
CviQI GTAC 5 cut(s) 33, 69, 355, 432, 749
DdeI CTNAG 4 cut(s) 111, 204, 468, 753
DpnI GATC 2 cut(s) 42, 483
DpnII GATC 2 cut(s) 40, 481
Ecl136II GAGCTC 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 717
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 468
Eco88I CYCGRG 2 cut(s) 258, 398
EcoICRI GAGCTC 1 cut(s) 715
EcoRII CCWGG 4 cut(s) 84, 213, 292, 345
EcoT38I GRGCYC 1 cut(s) 717
FaeI CATG 3 cut(s) 278, 331, 728
FaiI YATR 9 cut(s) 146, 276, 320, 329, 384, 497, 652, 690, 726
FaqI GGGAC 2 cut(s) 231, 252
FatI CATG 3 cut(s) 274, 327, 724
FauI CCCGC 1 cut(s) 431
FokI GGATG 2 cut(s) 110, 485
FriOI GRGCYC 1 cut(s) 717
FspBI CTAG 2 cut(s) 6, 668
GsuI CTGGAG 2 cut(s) 315, 387
Hin1II CATG 3 cut(s) 278, 331, 728
HinfI GANTC 2 cut(s) 254, 265
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 357, 581
Hpy188I TCNGA 6 cut(s) 19, 27, 253, 481, 571, 733
Hpy188III TCNNGA 5 cut(s) 49, 166, 191, 258, 467
Hpy8I GTNNAC 2 cut(s) 357, 581
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 2 cut(s) 32, 646
HpyCH4III ACNGT 3 cut(s) 431, 526, 748
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 557, 596, 721
HpyF3I CTNAG 4 cut(s) 111, 204, 468, 753
HpySE526I ACGT 1 cut(s) 583
Hsp92II CATG 3 cut(s) 278, 331, 728
Kzo9I GATC 2 cut(s) 40, 481
LmnI GCTCC 2 cut(s) 368, 721
LweI GCATC 2 cut(s) 88, 547
MabI ACCWGGT 1 cut(s) 345
MaeI CTAG 2 cut(s) 6, 668
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 1 cut(s) 61
MalI GATC 2 cut(s) 42, 483
MboI GATC 2 cut(s) 40, 481
MboII GAAGA 4 cut(s) 50, 272, 404, 673
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 3 cut(s) 122, 141, 604
MlyI GAGTC 1 cut(s) 274
MmeI TCCRAC 2 cut(s) 403, 756
MnlI CCTC 7 cut(s) 56, 67, 70, 280, 444, 463, 640
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 3 cut(s) 126, 135, 510
MslI CAYNNNNRTG 1 cut(s) 416
MspR9I CCNGG 4 cut(s) 86, 215, 294, 347
MvaI CCWGG 4 cut(s) 86, 215, 294, 347
MwoI GCNNNNNNNGC 4 cut(s) 209, 557, 596, 721
NdeII GATC 2 cut(s) 40, 481
NlaIII CATG 3 cut(s) 278, 331, 728
NlaIV GGNNCC 2 cut(s) 333, 730
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 1 cut(s) 278
PasI CCCWGGG 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 737
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 717
Psp1406I AACGTT 1 cut(s) 583
Psp6I CCWGG 4 cut(s) 84, 213, 292, 345
PspGI CCWGG 4 cut(s) 84, 213, 292, 345
PspN4I GGNNCC 2 cut(s) 333, 730
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 40
PsyI GACNNNGTC 1 cut(s) 737
RsaI GTAC 5 cut(s) 34, 70, 356, 433, 750
RsaNI GTAC 5 cut(s) 33, 69, 355, 432, 749
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 717
SaqAI TTAA 3 cut(s) 126, 135, 510
Sau3AI GATC 2 cut(s) 40, 481
ScaI AGTACT 2 cut(s) 34, 433
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 4 cut(s) 86, 215, 294, 347
SduI GDGCHC 1 cut(s) 717
SexAI ACCWGGT 1 cut(s) 345
SfaNI GCATC 2 cut(s) 88, 547
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 3 cut(s) 122, 141, 604
SsiI CCGC 1 cut(s) 438
SspMI CTAG 2 cut(s) 6, 668
SstI GAGCTC 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 84, 213, 292, 345
TaaI ACNGT 3 cut(s) 431, 526, 748
TaiI ACGT 1 cut(s) 586
TaqI TCGA 1 cut(s) 90
TasI AATT 3 cut(s) 122, 141, 604
TatI WGTACW 3 cut(s) 32, 354, 431
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 3 cut(s) 126, 135, 510
Tru9I TTAA 3 cut(s) 126, 135, 510
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 671
Tth111I GACNNNGTC 1 cut(s) 737
XapI RAATTY 2 cut(s) 122, 604
XceI RCATGY 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 2 cut(s) 6, 668
ZrmI AGTACT 2 cut(s) 34, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.