RchiOBHm_Chr5g0063851

carbonate dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000548
Physical Location & Seq
Reverse (-)
69633547 .. 69635865
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33992

Sequence Viewer

Length: 750 bp
ATGACTAGCGTGTTATCTGATCTGGTCAATACTGAAGATCTGCTCAAGACTGATCTGATCAGCACTGAGAATGAAAACCAGGATGATGTGGTTGCTACTGAAACTGAGAGCCTGGAAGATGACCCAGTTCAAACAATTTTAGACGGGTTCAAGCACTTCAAGACCAACTTTTACGACACAAATCCAGAGTTGTTCAATGAGCTTGCCCTAGCACAAAGCCCCAAGTTTTTGGTCTTTGCATGCTCGGATTCCCGAGTGAGTCCCTCGCATGTTCTTCATTGCACACCTGGACAAGCCTTTCTGGTTCGCAACATTGCTAACATGGTTCCTGCATATGACCAGCTAAGGTACACAGGAACTGGAGCAACCATTGAGTATGCTGTAGAAGAACTCGGGGTAGAAAACATTTTGATAGTGGGACACAGTAGTTGCGGAGGAATAAAAAGGCTAATGTCTTATCCCGAGGATGGTTCCGCTCCACTGGTCTTTATAGATGATTGGATGAAAATGGCCTTACCTGTCAAGACAAAGGTTATTGCGGAAGCTGGCGATACCGATTTCCAGGAACAATGTGATCGTTGCGCAAGGAAAGCAGTAAATTTGTCATTGGAAAACCTACTCACATATCCCTTCGTTCAAGAGGCACACTCGGAGAAAAAACTAGCACTTCGGGGTGCTTACTATGACTTTGTCAACGGACGTTTCGAGCTCTGGGAGCATGGTTCCGACAATATCATCATAGCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

249

Amino Acids

27.75

Weight (kDa)

4.51

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 77 - 234 1.4e-42 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 583
AccBSI CCGCTC 1 cut(s) 476
AciI CCGC 3 cut(s) 432, 474, 539
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 467
AgsI TTSAA 5 cut(s) 131, 151, 160, 196, 638
AjnI CCWGG 4 cut(s) 78, 111, 286, 561
AluBI AGCT 4 cut(s) 202, 343, 545, 709
AluI AGCT 4 cut(s) 202, 343, 545, 709
Alw21I GWGCWC 1 cut(s) 711
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 3 cut(s) 252, 392, 461
AoxI GGCC 1 cut(s) 510
ApoI RAATTY 1 cut(s) 598
AspLEI GCGC 1 cut(s) 584
AvaI CYCGRG 3 cut(s) 252, 392, 461
BanII GRGCYC 1 cut(s) 711
Bbv12I GWGCWC 1 cut(s) 711
BccI CCATC 1 cut(s) 461
BciT130I CCWGG 4 cut(s) 80, 113, 288, 563
BclI TGATCA 1 cut(s) 57
BfaI CTAG 3 cut(s) 6, 209, 662
BfmI CTRYAG 1 cut(s) 381
BglII AGATCT 1 cut(s) 37
Bme1390I CCNGG 4 cut(s) 80, 113, 288, 563
BmeT110I CYCGRG 3 cut(s) 252, 392, 461
BmiI GGNNCC 3 cut(s) 327, 472, 724
BmrFI CCNGG 4 cut(s) 80, 113, 288, 563
BmrI ACTGGG 1 cut(s) 119
BmuI ACTGGG 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 632, 664
BpmI CTGGAG 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 462
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse1I ACTGG 3 cut(s) 125, 364, 486
Bse3DI GCAATG 2 cut(s) 277, 312
BseBI CCWGG 4 cut(s) 80, 113, 288, 563
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 3 cut(s) 88, 472, 507
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMI GCAATG 2 cut(s) 277, 312
BseMII CTCAG 2 cut(s) 57, 96
BseNI ACTGG 3 cut(s) 125, 364, 486
BshFI GGCC 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 711
BsiHKCI CYCGRG 3 cut(s) 252, 392, 461
BslFI GGGAC 2 cut(s) 246, 432
BslI CCNNNNNNNGG 1 cut(s) 467
BsmFI GGGAC 2 cut(s) 246, 432
BsnI GGCC 1 cut(s) 512
BsoBI CYCGRG 3 cut(s) 252, 392, 461
Bsp1286I GDGCHC 1 cut(s) 711
Bsp143I GATC 5 cut(s) 19, 37, 52, 57, 574
BspACI CCGC 3 cut(s) 432, 474, 539
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 2 cut(s) 58, 97
BspLI GGNNCC 3 cut(s) 327, 472, 724
BsrBI CCGCTC 1 cut(s) 476
BsrDI GCAATG 2 cut(s) 277, 312
BsrI ACTGG 3 cut(s) 125, 364, 486
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 5 cut(s) 19, 37, 52, 57, 574
Bst2UI CCWGG 4 cut(s) 80, 113, 288, 563
Bst4CI ACNGT 1 cut(s) 425
BstC8I GCNNGC 3 cut(s) 204, 241, 547
BstDEI CTNAG 4 cut(s) 66, 105, 344, 747
BstF5I GGATG 3 cut(s) 88, 472, 507
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 5 cut(s) 22, 40, 55, 60, 577
BstMBI GATC 5 cut(s) 19, 37, 52, 57, 574
BstMWI GCNNNNNNNGC 2 cut(s) 590, 715
BstNI CCWGG 4 cut(s) 80, 113, 288, 563
BstNSI RCATGY 2 cut(s) 243, 272
BstSCI CCNGG 4 cut(s) 78, 111, 286, 561
BstSFI CTRYAG 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 37
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 1 cut(s) 512
BtsCI GGATG 3 cut(s) 88, 472, 507
BtsIMutI CAGTG 2 cut(s) 63, 479
Cac8I GCNNGC 3 cut(s) 204, 241, 547
CaiI CAGNNNCTG 1 cut(s) 359
CfoI GCGC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 349
CviAII CATG 4 cut(s) 240, 269, 322, 719
CviJI RGCY 9 cut(s) 111, 202, 219, 296, 343, 448, 512, 545, 709
CviKI_1 RGCY 9 cut(s) 111, 202, 219, 296, 343, 448, 512, 545, 709
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 4 cut(s) 66, 105, 344, 747
DpnI GATC 5 cut(s) 21, 39, 54, 59, 576
DpnII GATC 5 cut(s) 19, 37, 52, 57, 574
Ecl136II GAGCTC 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 711
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 3 cut(s) 252, 392, 461
EcoICRI GAGCTC 1 cut(s) 709
EcoRII CCWGG 4 cut(s) 78, 111, 286, 561
EcoT38I GRGCYC 1 cut(s) 711
FaeI CATG 4 cut(s) 243, 272, 325, 722
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 2 cut(s) 246, 432
FatI CATG 4 cut(s) 239, 268, 321, 718
FauNDI CATATG 1 cut(s) 334
FbaI TGATCA 1 cut(s) 57
FokI GGATG 3 cut(s) 95, 479, 514
FriOI GRGCYC 1 cut(s) 711
FspBI CTAG 3 cut(s) 6, 209, 662
FspI TGCGCA 1 cut(s) 583
GlaI GCGC 1 cut(s) 583
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 512
HhaI GCGC 1 cut(s) 584
Hin1II CATG 4 cut(s) 243, 272, 325, 722
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HinfI GANTC 2 cut(s) 248, 259
Hpy166II GTNNAC 2 cut(s) 351, 694
Hpy188I TCNGA 5 cut(s) 19, 57, 247, 652, 727
Hpy188III TCNNGA 7 cut(s) 46, 160, 185, 252, 461, 523, 638
Hpy8I GTNNAC 2 cut(s) 351, 694
HpyAV CCTTC 1 cut(s) 640
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 700
HpyCH4V TGCA 3 cut(s) 239, 282, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 715
HpyF3I CTNAG 4 cut(s) 66, 105, 344, 747
HpySE526I ACGT 1 cut(s) 700
Hsp92II CATG 4 cut(s) 243, 272, 325, 722
HspAI GCGC 1 cut(s) 582
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 5 cut(s) 19, 37, 52, 57, 574
LmnI GCTCC 3 cut(s) 362, 481, 715
MaeI CTAG 3 cut(s) 6, 209, 662
MaeII ACGT 1 cut(s) 700
MalI GATC 5 cut(s) 21, 39, 54, 59, 576
MbiI CCGCTC 1 cut(s) 476
MboI GATC 5 cut(s) 19, 37, 52, 57, 574
MboII GAAGA 4 cut(s) 47, 128, 266, 398
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 711
MluCI AATT 2 cut(s) 135, 598
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 750
MnlI CCTC 4 cut(s) 274, 428, 457, 634
MspR9I CCNGG 4 cut(s) 80, 113, 288, 563
MvaI CCWGG 4 cut(s) 80, 113, 288, 563
MwoI GCNNNNNNNGC 2 cut(s) 590, 715
NdeI CATATG 1 cut(s) 334
NdeII GATC 5 cut(s) 19, 37, 52, 57, 574
NlaIII CATG 4 cut(s) 243, 272, 325, 722
NlaIV GGNNCC 3 cut(s) 327, 472, 724
NsbI TGCGCA 1 cut(s) 583
NspI RCATGY 2 cut(s) 243, 272
PaeI GCATGC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 702
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 561
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 4 cut(s) 78, 111, 286, 561
PspGI CCWGG 4 cut(s) 78, 111, 286, 561
PspN4I GGNNCC 3 cut(s) 327, 472, 724
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 689
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SacI GAGCTC 1 cut(s) 711
Sau3AI GATC 5 cut(s) 19, 37, 52, 57, 574
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 4 cut(s) 80, 113, 288, 563
SduI GDGCHC 1 cut(s) 711
SfcI CTRYAG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SphI GCATGC 1 cut(s) 243
Sse9I AATT 2 cut(s) 135, 598
SsiI CCGC 3 cut(s) 432, 474, 539
SspMI CTAG 3 cut(s) 6, 209, 662
SstI GAGCTC 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 78, 111, 286, 561
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 1 cut(s) 703
TaqI TCGA 1 cut(s) 705
TasI AATT 2 cut(s) 135, 598
TfiI GAWTC 1 cut(s) 248
TscAI CASTG 2 cut(s) 70, 486
TspDTI ATGAA 3 cut(s) 87, 266, 518
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 2 cut(s) 70, 486
Tth111I GACNNNGTC 1 cut(s) 689
XapI RAATTY 1 cut(s) 598
XceI RCATGY 2 cut(s) 243, 272
XspI CTAG 3 cut(s) 6, 209, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.