Rw5G039370

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000548
Physical Location & Seq
Reverse (-)
69445442 .. 69447227
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039370.1

Sequence Viewer

Length: 831 bp
ATGACTAGCCAGTTATCTGATTTGACCAACATTCAAGATCTCATCTTGAAAAAGCTTGTGAGGTTAGTCCATTATCAGAAGGAAGACCTGGAAGATGTAGCTGCTCCCAAACTTCAGAACCTCAAGGTTGAGTGCAATGACCTGAAAGGAGCTGAACCAAATACACCGACCAGCCCAATAGCCAGCCCTCTCGACCCAGTTAAAAGAATTATAGATGGCTTCAAGTACTTCAAGACCAGCTACTTCGACACAAAACCAGCTTTGTTTGATGAGCTTGCCCAAGGACAAAGCCCCGAGTTTATGGTATTTGCATGCTCGGATTCTCGAGTGTGTCCTTCACATGTTCTTCACTTCCAACCTGGGGAGGCCTTCATCGTTCGCAACATTGCTAACATGGTTCCTGCTTTTGATCAGCTAAAACATGCAGGAGTTGGAGCAACCATAGAATATGCTGTCAAAGAACTCGGGGTGGCAAATATTTTGGTAATGGGACACAGTAATTGTGGTGGAATAAAGAGGCTAATGTCTTATCCGGAGGATGGCTCTGAACCCTTCGACTTCATAGATGAATGGGTGAAAATGATTTTACCAGCCAAGGCTGAGGTTATTGCAGAAGCTGGGAGTGCAGATTTCCACGAACAATGTGAAAGATGTGCAAGGGAATCAGTAAACTTGTCACTGGCAAACCTACTTACCTACCCCTTTGTTCAAAAGGCACACTTGGATGAAAAACTAGCACTTCGGGGTGGATACCATGACTTTGTCAATGGAATTTTCGAGCTATGGGAGCTCAAATCCCAAATTTTAAACCCCATCATCGTACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

276

Amino Acids

30.77

Weight (kDa)

5.2

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 101 - 257 2.3e-41 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 532
AcsI RAATTY 2 cut(s) 771, 801
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 2 cut(s) 227, 822
AfiI CCNNNNNNNGG 2 cut(s) 361, 539
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 5 cut(s) 35, 49, 223, 232, 710
AjnI CCWGG 2 cut(s) 87, 358
Alw21I GWGCWC 1 cut(s) 792
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 3 cut(s) 293, 324, 464
Aor13HI TCCGGA 1 cut(s) 532
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 2 cut(s) 771, 801
AsuHPI GGTGA 1 cut(s) 586
AvaI CYCGRG 3 cut(s) 293, 324, 464
BanII GRGCYC 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 792
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 1 cut(s) 88
BccI CCATC 3 cut(s) 209, 533, 821
BciT130I CCWGG 2 cut(s) 89, 360
BciVI GTATCC 1 cut(s) 743
BclI TGATCA 1 cut(s) 409
BfaI CTAG 2 cut(s) 6, 734
BfuI GTATCC 1 cut(s) 743
BglII AGATCT 1 cut(s) 37
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 2 cut(s) 89, 360
BmeT110I CYCGRG 3 cut(s) 293, 324, 464
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 2 cut(s) 89, 360
BmrI ACTGGG 1 cut(s) 191
BmuI ACTGGG 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 527, 559
Bpu10I CCTNAGC 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 280, 359, 594
BsaWI WCCGGW 1 cut(s) 532
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 539
Bse1I ACTGG 3 cut(s) 10, 197, 684
Bse3DI GCAATG 2 cut(s) 142, 384
BseAI TCCGGA 1 cut(s) 532
BseBI CCWGG 2 cut(s) 89, 360
BseDI CCNNGG 3 cut(s) 280, 359, 594
BseGI GGATG 2 cut(s) 544, 730
BseLI CCNNNNNNNGG 2 cut(s) 361, 539
BseMI GCAATG 2 cut(s) 142, 384
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 3 cut(s) 10, 197, 684
BseXI GCAGC 1 cut(s) 88
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 1 cut(s) 645
BshFI GGCC 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 792
BsiHKCI CYCGRG 3 cut(s) 293, 324, 464
BsiSI CCGG 1 cut(s) 533
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 2 cut(s) 361, 539
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 1 cut(s) 368
BsoBI CYCGRG 3 cut(s) 293, 324, 464
Bsp1286I GDGCHC 1 cut(s) 792
Bsp13I TCCGGA 1 cut(s) 532
Bsp143I GATC 2 cut(s) 37, 409
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 1 cut(s) 592
BspEI TCCGGA 1 cut(s) 532
BspLI GGNNCC 1 cut(s) 399
BsrDI GCAATG 2 cut(s) 142, 384
BsrI ACTGG 3 cut(s) 10, 197, 684
BssECI CCNNGG 3 cut(s) 280, 359, 594
BssMI GATC 2 cut(s) 37, 409
BssT1I CCWWGG 2 cut(s) 280, 594
Bst2UI CCWGG 2 cut(s) 89, 360
Bst4CI ACNGT 1 cut(s) 497
BstC8I GCNNGC 3 cut(s) 184, 276, 313
BstDEI CTNAG 1 cut(s) 600
BstF5I GGATG 2 cut(s) 544, 730
BstKTI GATC 2 cut(s) 40, 412
BstMBI GATC 2 cut(s) 37, 409
BstMWI GCNNNNNNNGC 2 cut(s) 623, 787
BstNI CCWGG 2 cut(s) 89, 360
BstNSI RCATGY 3 cut(s) 315, 344, 425
BstSCI CCNGG 2 cut(s) 87, 358
BstV1I GCAGC 1 cut(s) 88
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 1 cut(s) 743
BsuRI GGCC 1 cut(s) 368
BtsCI GGATG 2 cut(s) 544, 730
BtsIMutI CAGTG 1 cut(s) 677
Cac8I GCNNGC 3 cut(s) 184, 276, 313
CaiI CAGNNNCTG 1 cut(s) 617
Csp6I GTAC 2 cut(s) 226, 821
CviAII CATG 5 cut(s) 312, 341, 394, 422, 755
CviQI GTAC 2 cut(s) 226, 821
DdeI CTNAG 1 cut(s) 600
DpnI GATC 2 cut(s) 39, 411
DpnII GATC 2 cut(s) 37, 409
DraI TTTAAA 1 cut(s) 807
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 2 cut(s) 280, 594
Eco147I AGGCCT 1 cut(s) 368
Eco24I GRGCYC 1 cut(s) 792
Eco53kI GAGCTC 1 cut(s) 790
Eco57I CTGAAG 1 cut(s) 98
Eco88I CYCGRG 3 cut(s) 293, 324, 464
EcoICRI GAGCTC 1 cut(s) 790
EcoRII CCWGG 2 cut(s) 87, 358
EcoT14I CCWWGG 2 cut(s) 280, 594
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 2 cut(s) 280, 594
FaeI CATG 5 cut(s) 315, 344, 397, 425, 758
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 1 cut(s) 504
FatI CATG 5 cut(s) 311, 340, 393, 421, 754
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 2 cut(s) 551, 737
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 1 cut(s) 102
FspBI CTAG 2 cut(s) 6, 734
GluI GCNGC 1 cut(s) 102
GsaI CCCAGC 1 cut(s) 621
HaeIII GGCC 1 cut(s) 368
HapII CCGG 1 cut(s) 533
Hin1II CATG 5 cut(s) 315, 344, 397, 425, 758
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 320, 662
HpaII CCGG 1 cut(s) 533
HphI GGTGA 1 cut(s) 586
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 5 cut(s) 19, 78, 117, 319, 547
Hpy188III TCNNGA 7 cut(s) 35, 46, 191, 232, 324, 533, 828
Hpy8I GTNNAC 1 cut(s) 670
HpyAV CCTTC 4 cut(s) 73, 345, 379, 562
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4V TGCA 6 cut(s) 135, 311, 425, 611, 626, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 623, 787
HpyF3I CTNAG 1 cut(s) 600
Hsp92II CATG 5 cut(s) 315, 344, 397, 425, 758
Kpn2I TCCGGA 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 2 cut(s) 37, 409
LmnI GCTCC 4 cut(s) 109, 149, 434, 787
Lsp1109I GCAGC 1 cut(s) 88
MaeI CTAG 2 cut(s) 6, 734
MaeIII GTNAC 1 cut(s) 675
MalI GATC 2 cut(s) 39, 411
MboI GATC 2 cut(s) 37, 409
MboII GAAGA 3 cut(s) 95, 104, 338
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 4 cut(s) 207, 499, 771, 801
MmeI TCCRAC 2 cut(s) 379, 412
MnlI CCTC 7 cut(s) 54, 131, 198, 358, 510, 529, 595
MroI TCCGGA 1 cut(s) 532
MseI TTAA 2 cut(s) 201, 806
MslI CAYNNNNRTG 1 cut(s) 723
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 2 cut(s) 89, 360
MvaI CCWGG 2 cut(s) 89, 360
MwoI GCNNNNNNNGC 2 cut(s) 623, 787
NdeII GATC 2 cut(s) 37, 409
NlaIII CATG 5 cut(s) 315, 344, 397, 425, 758
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 675
NspI RCATGY 3 cut(s) 315, 344, 425
PaeI GCATGC 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 324
PceI AGGCCT 1 cut(s) 368
PciI ACATGT 1 cut(s) 340
PfeI GAWTC 2 cut(s) 320, 662
PflFI GACNNNGTC 1 cut(s) 761
PkrI GCNGC 1 cut(s) 103
PscI ACATGT 1 cut(s) 340
Psp124BI GAGCTC 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 87, 358
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 2 cut(s) 87, 358
PspN4I GGNNCC 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 761
RsaI GTAC 2 cut(s) 227, 822
RsaNI GTAC 2 cut(s) 226, 821
RseI CAYNNNNRTG 1 cut(s) 723
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 2 cut(s) 201, 806
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 2 cut(s) 37, 409
ScaI AGTACT 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 89, 360
SduI GDGCHC 1 cut(s) 792
Sfr274I CTCGAG 1 cut(s) 324
SlaI CTCGAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 2 cut(s) 122, 324
SmoI CTYRAG 2 cut(s) 122, 324
SphI GCATGC 1 cut(s) 315
Sse9I AATT 4 cut(s) 207, 499, 771, 801
SseBI AGGCCT 1 cut(s) 368
SspI AATATT 1 cut(s) 478
SspMI CTAG 2 cut(s) 6, 734
SstI GAGCTC 1 cut(s) 792
StuI AGGCCT 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 87, 358
StyI CCWWGG 2 cut(s) 280, 594
TaaI ACNGT 1 cut(s) 497
TaqI TCGA 5 cut(s) 192, 246, 325, 555, 777
TasI AATT 4 cut(s) 207, 499, 771, 801
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 2 cut(s) 320, 662
Tru1I TTAA 2 cut(s) 201, 806
Tru9I TTAA 2 cut(s) 201, 806
TscAI CASTG 1 cut(s) 684
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 4 cut(s) 361, 550, 582, 741
TspRI CASTG 1 cut(s) 684
Tth111I GACNNNGTC 1 cut(s) 761
XapI RAATTY 2 cut(s) 771, 801
XceI RCATGY 3 cut(s) 315, 344, 425
XhoI CTCGAG 1 cut(s) 324
XspI CTAG 2 cut(s) 6, 734
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.