Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000548

Physical Location & Seq

Genomic Coordinates Forward (+)

2462330 .. 2465106

Gene Structure 2777 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1034000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGTCTTCAGCTTCGTTAGTTAAGAGCTTTAACCTCACCTCCTCCGCCCTCTCTTCCGCTACCAGCAGGAAACCTAGTAATATTTGTGCCAGGATCAGCACTTCTTCTTCTCCTTGTAGATCTCCTCCCCCTAACCTAATCCAAAACAAGCCTGTTACCTCCGTTCCTGCTATCACTCGGAAGGAAGAAATGTCAAAGGAGTACGAAGAGGCCATTGCATCTCTCCAGAAACTTCTCAGTGAGAAGGAGGAGCTGAGGGCTGAAGCGGCTGCGAAAGTGGAGCAGGTAACAGCTCAGCTGGCGACGGCCGAGGCCAACGGCGCCACCAAGCCTTATGATGCCGCCGAGAGGCTCAAGAGCGGCTTCATTTACTTCAAGAAAGAGAAATACGACAAGAATCCGGCTCTTTACGGTGAACTTGCCAAAGGACAATGGCCCAAGTTTATGCTGTTTGCATGCTCGGATTCTCGAGTCTGTCCTTCACATGTTCTGGATATGCAGCCAGGAGAGGCTTTTGTTGTCCGCACAGTTGCTAACTTGGTCCCACCCTATGATAAGACTAAATACGCTGGAACTGGGGCGGCCGTTGAGTATGCTGTCTTGCATCTCAAGGTTTCATACATAGTGGTGATTGGGCACAGCGCCTGTGGAGGAATCAAGGCGCTCTTGTCGATTCCAGAAGATGGGTCTACCCTTGGCACTGACTTCATAGAAGACTGGATTGGTATCGCATCGCCGGCAGGGAAGAAGGTGAAGGCAGATCATGGTGCCGATGCTCCTTTCGCTGATCTATGCGGCCACTGTGAAAAGGAGGCGGTGAATGTATCGATCGGAAACCTGCTGAGCTATCCGTTTGTGAGAGAGGGTTTGCTGAAGAAAACCCTAGCAATCAAGGGAGGATACTATGACTTTGTCAACGGACGTTTCGAGCTCTGGGATGTTGATTTCAGCCTCAACCCCATCTTCTCCGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004089 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009814 GO:0009817 GO:0009941 GO:0009987 GO:0010033 GO:0010037 GO:0010119 GO:0010319 GO:0015979 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042221 GO:0042493 GO:0042651 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048046 GO:0048519 GO:0048580 GO:0048581 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051704 GO:0051707 GO:0055035 GO:0065007 GO:0071944 GO:0072347 GO:0098542 GO:1901700 GO:2000026 GO:2000038 GO:2000122

KEGG Pathways

Metabolic & Signaling

ko00910 map00910

Pfam Domains

Protein Families

Pro_CA

Protein Analysis

325

Amino Acids

35.01

Weight (kDa)

7.55

Isoelectric Point (pI)

39.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pro_CA	PF00484	149 - 308	9.9e-41	Carbonic anhydrase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000548)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca	FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica	MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica	Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis	pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis	RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata	RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora	Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii	Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa	Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha	Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana	Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	274, 846
AccB1I	GGYRCC	2 cut(s)	320, 767
AccB7I	CCANNNNNTGG	1 cut(s)	683
AccBSI	CCGCTC	1 cut(s)	360
AccI	GTMKAC	1 cut(s)	689
AciI	CCGC	9 cut(s)	45, 57, 266, 342, 360, 523, 581, 795, 815
AclWI	GGATC	1 cut(s)	101
AcoI	YGGCCR	3 cut(s)	306, 582, 796
AcuI	CTGAAG	2 cut(s)	282, 893
AcyI	GRCGYC	1 cut(s)	321
AfaI	GTAC	1 cut(s)	203
AfiI	CCNNNNNNNGG	2 cut(s)	348, 683
AflIII	ACRYGT	1 cut(s)	484
AgsI	TTSAA	1 cut(s)	376
AjnI	CCWGG	2 cut(s)	89, 502
AjuI	GAANNNNNNNTTGG	2 cut(s)	705, 737
AluBI	AGCT	7 cut(s)	11, 27, 253, 293, 298, 846, 931
AluI	AGCT	7 cut(s)	11, 27, 253, 293, 298, 846, 931
Alw21I	GWGCWC	1 cut(s)	933
AlwI	GGATC	1 cut(s)	101
AlwNI	CAGNNNCTG	1 cut(s)	645
Ama87I	CYCGRG	1 cut(s)	468
AoxI	GGCC	6 cut(s)	210, 306, 312, 434, 582, 796
ApeKI	GCWGC	2 cut(s)	269, 499
AspLEI	GCGC	3 cut(s)	323, 644, 664
AspS9I	GGNCC	2 cut(s)	435, 541
AsuHPI	GGTGA	5 cut(s)	28, 425, 640, 763, 829
AvaI	CYCGRG	1 cut(s)	468
AvaII	GGWCC	1 cut(s)	541
BaeGI	GKGCMC	1 cut(s)	639
BanI	GGYRCC	2 cut(s)	320, 767
BanII	GRGCYC	1 cut(s)	933
BbsI	GAAGAC	1 cut(s)	720
Bbv12I	GWGCWC	1 cut(s)	933
BbvCI	CCTCAGC	1 cut(s)	254
BbvI	GCAGC	2 cut(s)	256, 511
BccI	CCATC	2 cut(s)	677, 968
BceAI	ACGGC	3 cut(s)	321, 334, 569
BciT130I	CCWGG	2 cut(s)	91, 504
BciVI	GTATCC	1 cut(s)	893
BfaI	CTAG	2 cut(s)	75, 884
BfoI	RGCGCY	3 cut(s)	324, 645, 665
BfuAI	ACCTGC	2 cut(s)	274, 846
BfuI	GTATCC	1 cut(s)	893
BglII	AGATCT	1 cut(s)	119
BisI	GCNGC	7 cut(s)	267, 270, 342, 361, 500, 582, 796
BlpI	GCTNAGC	2 cut(s)	294, 842
BlsI	GCNGC	7 cut(s)	268, 271, 343, 362, 501, 583, 797
Bme1390I	CCNGG	2 cut(s)	91, 504
Bme18I	GGWCC	1 cut(s)	541
BmeT110I	CYCGRG	1 cut(s)	468
BmgT120I	GGNCC	2 cut(s)	435, 541
BmiI	GGNNCC	3 cut(s)	322, 543, 769
BmrFI	CCNGG	2 cut(s)	91, 504
BmrI	ACTGGG	1 cut(s)	585
BmsI	GCATC	5 cut(s)	227, 328, 613, 740, 763
BmuI	ACTGGG	1 cut(s)	585
BpiI	GAAGAC	1 cut(s)	720
BpmI	CTGGAG	1 cut(s)	209
Bpu10I	CCTNAGC	1 cut(s)	254
Bpu1102I	GCTNAGC	2 cut(s)	294, 842
BpuEI	CTTGAG	2 cut(s)	338, 593
Bsa29I	ATCGAT	1 cut(s)	827
BsaBI	GATNNNNATC	1 cut(s)	725
BsaHI	GRCGYC	1 cut(s)	321
BsaJI	CCNNGG	2 cut(s)	309, 694
BsaXI	ACNNNNNCTCC	2 cut(s)	23, 53
Bsc4I	CCNNNNNNNGG	2 cut(s)	348, 683
Bse118I	RCCGGY	1 cut(s)	736
Bse1I	ACTGG	2 cut(s)	580, 722
Bse3DI	GCAATG	1 cut(s)	213
Bse8I	GATNNNNATC	1 cut(s)	725
BseBI	CCWGG	2 cut(s)	91, 504
BseCI	ATCGAT	1 cut(s)	827
BseDI	CCNNGG	2 cut(s)	309, 694
BseGI	GGATG	1 cut(s)	943
BseJI	GATNNNNATC	1 cut(s)	725
BseLI	CCNNNNNNNGG	2 cut(s)	348, 683
BseMI	GCAATG	1 cut(s)	213
BseMII	CTCAG	4 cut(s)	245, 250, 308, 833
BseNI	ACTGG	2 cut(s)	580, 722
BseRI	GAGGAG	3 cut(s)	31, 114, 263
BseSI	GKGCMC	1 cut(s)	639
BseX3I	CGGCCG	2 cut(s)	306, 582
BseXI	GCAGC	2 cut(s)	256, 511
Bsh1285I	CGRYCG	3 cut(s)	309, 585, 831
BshFI	GGCC	6 cut(s)	212, 308, 314, 436, 584, 798
BshNI	GGYRCC	2 cut(s)	320, 767
BshVI	ATCGAT	1 cut(s)	827
BsiEI	CGRYCG	3 cut(s)	309, 585, 831
BsiHKAI	GWGCWC	1 cut(s)	933
BsiHKCI	CYCGRG	1 cut(s)	468
BsiSI	CCGG	2 cut(s)	401, 737
BslFI	GGGAC	1 cut(s)	527
BslI	CCNNNNNNNGG	2 cut(s)	348, 683
BsmFI	GGGAC	1 cut(s)	527
BsnI	GGCC	6 cut(s)	212, 308, 314, 436, 584, 798
BsoBI	CYCGRG	1 cut(s)	468
Bsp1286I	GDGCHC	2 cut(s)	639, 933
Bsp143I	GATC	5 cut(s)	93, 119, 760, 787, 828
Bsp1720I	GCTNAGC	2 cut(s)	294, 842
BspACI	CCGC	9 cut(s)	45, 57, 266, 342, 360, 523, 581, 795, 815
BspANI	GGCC	6 cut(s)	212, 308, 314, 436, 584, 798
BspCNI	CTCAG	4 cut(s)	246, 249, 307, 834
BspDI	ATCGAT	1 cut(s)	827
BspLI	GGNNCC	3 cut(s)	322, 543, 769
BspMI	ACCTGC	2 cut(s)	274, 846
BspPI	GGATC	1 cut(s)	101
BspT107I	GGYRCC	2 cut(s)	320, 767
BsrBI	CCGCTC	1 cut(s)	360
BsrDI	GCAATG	1 cut(s)	213
BsrFI	RCCGGY	1 cut(s)	736
BsrI	ACTGG	2 cut(s)	580, 722
BssAI	RCCGGY	1 cut(s)	736
BssECI	CCNNGG	2 cut(s)	309, 694
BssMI	GATC	5 cut(s)	93, 119, 760, 787, 828
BssNI	GRCGYC	1 cut(s)	321
BssT1I	CCWWGG	1 cut(s)	694
Bst2UI	CCWGG	2 cut(s)	91, 504
Bst4CI	ACNGT	3 cut(s)	413, 529, 803
Bst6I	CTCTTC	2 cut(s)	58, 201
BstACI	GRCGYC	1 cut(s)	321
BstC8I	GCNNGC	3 cut(s)	300, 457, 738
BstDEI	CTNAG	4 cut(s)	236, 254, 294, 842
BstF5I	GGATG	1 cut(s)	943
BstH2I	RGCGCY	3 cut(s)	324, 645, 665
BstHHI	GCGC	3 cut(s)	323, 644, 664
BstKTI	GATC	5 cut(s)	96, 122, 763, 790, 831
BstMBI	GATC	5 cut(s)	93, 119, 760, 787, 828
BstMCI	CGRYCG	3 cut(s)	309, 585, 831
BstMWI	GCNNNNNNNGC	5 cut(s)	266, 299, 320, 737, 782
BstNI	CCWGG	2 cut(s)	91, 504
BstNSI	RCATGY	2 cut(s)	459, 488
BstSCI	CCNGG	2 cut(s)	89, 502
BstSLI	GKGCMC	1 cut(s)	639
BstV1I	GCAGC	2 cut(s)	256, 511
BstV2I	GAAGAC	1 cut(s)	720
BstX2I	RGATCY	1 cut(s)	119
BstYI	RGATCY	1 cut(s)	119
BstZI	CGGCCG	2 cut(s)	306, 582
Bsu15I	ATCGAT	1 cut(s)	827
BsuI	GTATCC	1 cut(s)	893
BsuRI	GGCC	6 cut(s)	212, 308, 314, 436, 584, 798
BsuTUI	ATCGAT	1 cut(s)	827
BtgZI	GCGATG	1 cut(s)	717
BtsCI	GGATG	1 cut(s)	943
BtsIMutI	CAGTG	3 cut(s)	244, 699, 799
BveI	ACCTGC	2 cut(s)	274, 846
Cac8I	GCNNGC	3 cut(s)	300, 457, 738
CaiI	CAGNNNCTG	1 cut(s)	645
CfoI	GCGC	3 cut(s)	323, 644, 664
Cfr10I	RCCGGY	1 cut(s)	736
Cfr13I	GGNCC	2 cut(s)	435, 541
ClaI	ATCGAT	1 cut(s)	827
Csp6I	GTAC	1 cut(s)	202
CviAII	CATG	3 cut(s)	456, 485, 764
CviQI	GTAC	1 cut(s)	202
DdeI	CTNAG	4 cut(s)	236, 254, 294, 842
DinI	GGCGCC	1 cut(s)	322
DpnI	GATC	5 cut(s)	95, 121, 762, 789, 830
DpnII	GATC	5 cut(s)	93, 119, 760, 787, 828
EaeI	YGGCCR	3 cut(s)	306, 582, 796
EagI	CGGCCG	2 cut(s)	306, 582
Eam1104I	CTCTTC	2 cut(s)	58, 201
EarI	CTCTTC	2 cut(s)	58, 201
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	1 cut(s)	931
EclXI	CGGCCG	2 cut(s)	306, 582
Eco130I	CCWWGG	1 cut(s)	694
Eco24I	GRGCYC	1 cut(s)	933
Eco47I	GGWCC	1 cut(s)	541
Eco52I	CGGCCG	2 cut(s)	306, 582
Eco53kI	GAGCTC	1 cut(s)	931
Eco57I	CTGAAG	2 cut(s)	282, 893
Eco88I	CYCGRG	1 cut(s)	468
EcoICRI	GAGCTC	1 cut(s)	931
EcoRII	CCWGG	2 cut(s)	89, 502
EcoT14I	CCWWGG	1 cut(s)	694
EcoT38I	GRGCYC	1 cut(s)	933
EgeI	GGCGCC	1 cut(s)	322
EheI	GGCGCC	1 cut(s)	322
ErhI	CCWWGG	1 cut(s)	694
FaeI	CATG	3 cut(s)	459, 488, 767
FalI	AAGNNNNNCTT	4 cut(s)	347, 379, 650, 682
FaqI	GGGAC	1 cut(s)	527
FatI	CATG	3 cut(s)	455, 484, 763
FblI	GTMKAC	1 cut(s)	689
Fnu4HI	GCNGC	7 cut(s)	267, 270, 342, 361, 500, 582, 796
FokI	GGATG	1 cut(s)	950
FriOI	GRGCYC	1 cut(s)	933
Fsp4HI	GCNGC	7 cut(s)	267, 270, 342, 361, 500, 582, 796
FspBI	CTAG	2 cut(s)	75, 884
GlaI	GCGC	3 cut(s)	322, 643, 663
GluI	GCNGC	7 cut(s)	267, 270, 342, 361, 500, 582, 796
GsuI	CTGGAG	1 cut(s)	209
HaeII	RGCGCY	3 cut(s)	324, 645, 665
HaeIII	GGCC	6 cut(s)	212, 308, 314, 436, 584, 798
HapII	CCGG	2 cut(s)	401, 737
HhaI	GCGC	3 cut(s)	323, 644, 664
Hin1I	GRCGYC	1 cut(s)	321
Hin1II	CATG	3 cut(s)	459, 488, 767
Hin6I	GCGC	3 cut(s)	321, 642, 662
HinP1I	GCGC	3 cut(s)	321, 642, 662
HincII	GTYRAC	1 cut(s)	916
HindII	GTYRAC	1 cut(s)	916
HinfI	GANTC	5 cut(s)	397, 464, 471, 654, 673
HpaII	CCGG	2 cut(s)	401, 737
HphI	GGTGA	5 cut(s)	28, 425, 640, 763, 829
Hpy166II	GTNNAC	3 cut(s)	416, 690, 916
Hpy188I	TCNGA	3 cut(s)	180, 463, 833
Hpy188III	TCNNGA	6 cut(s)	226, 355, 376, 468, 491, 677
Hpy8I	GTNNAC	3 cut(s)	416, 690, 916
Hpy99I	CGWCG	1 cut(s)	307
HpyAV	CCTTC	5 cut(s)	175, 238, 489, 742, 748
HpyCH4III	ACNGT	3 cut(s)	413, 529, 803
HpyCH4IV	ACGT	1 cut(s)	922
HpyCH4V	TGCA	4 cut(s)	218, 455, 499, 604
HpyF10VI	GCNNNNNNNGC	5 cut(s)	266, 299, 320, 737, 782
HpyF3I	CTNAG	4 cut(s)	236, 254, 294, 842
HpySE526I	ACGT	1 cut(s)	922
Hsp92I	GRCGYC	1 cut(s)	321
Hsp92II	CATG	3 cut(s)	459, 488, 767
HspAI	GCGC	3 cut(s)	321, 642, 662
KasI	GGCGCC	1 cut(s)	320
KroI	GCCGGC	1 cut(s)	736
KroNI	GCCGGC	1 cut(s)	738
Kzo9I	GATC	5 cut(s)	93, 119, 760, 787, 828
LmnI	GCTCC	3 cut(s)	250, 280, 781
Lsp1109I	GCAGC	2 cut(s)	256, 511
LweI	GCATC	5 cut(s)	227, 328, 613, 740, 763
MaeI	CTAG	2 cut(s)	75, 884
MaeII	ACGT	1 cut(s)	922
MaeIII	GTNAC	2 cut(s)	154, 286
MalI	GATC	5 cut(s)	95, 121, 762, 789, 830
MbiI	CCGCTC	1 cut(s)	360
MboI	GATC	5 cut(s)	93, 119, 760, 787, 828
MflI	RGATCY	1 cut(s)	119
MhlI	GDGCHC	2 cut(s)	639, 933
Mly113I	GGCGCC	1 cut(s)	321
MlyI	GAGTC	1 cut(s)	480
MroNI	GCCGGC	1 cut(s)	736
MseI	TTAA	2 cut(s)	21, 30
MslI	CAYNNNNRTG	1 cut(s)	626
MspA1I	CMGCKG	1 cut(s)	298
MspI	CCGG	2 cut(s)	401, 737
MspR9I	CCNGG	2 cut(s)	91, 504
MvaI	CCWGG	2 cut(s)	91, 504
MwoI	GCNNNNNNNGC	5 cut(s)	266, 299, 320, 737, 782
NaeI	GCCGGC	1 cut(s)	738
NarI	GGCGCC	1 cut(s)	321
NdeII	GATC	5 cut(s)	93, 119, 760, 787, 828
NgoMIV	GCCGGC	1 cut(s)	736
NlaIII	CATG	3 cut(s)	459, 488, 767
NlaIV	GGNNCC	3 cut(s)	322, 543, 769
NmeAIII	GCCGAG	2 cut(s)	334, 370
NspI	RCATGY	2 cut(s)	459, 488
PaeI	GCATGC	1 cut(s)	459
PaeR7I	CTCGAG	1 cut(s)	468
PciI	ACATGT	1 cut(s)	484
PcsI	WCGNNNNNNNCGW	1 cut(s)	924
PdiI	GCCGGC	1 cut(s)	738
PfeI	GAWTC	4 cut(s)	397, 464, 654, 673
PflFI	GACNNNGTC	1 cut(s)	911
PflMI	CCANNNNNTGG	1 cut(s)	683
PkrI	GCNGC	7 cut(s)	268, 271, 343, 362, 501, 583, 797
Ple19I	CGATCG	1 cut(s)	831
PleI	GAGTC	1 cut(s)	479
PluTI	GGCGCC	1 cut(s)	324
PpsI	GAGTC	1 cut(s)	479
PscI	ACATGT	1 cut(s)	484
Psp124BI	GAGCTC	1 cut(s)	933
Psp6I	CCWGG	2 cut(s)	89, 502
PspGI	CCWGG	2 cut(s)	89, 502
PspN4I	GGNNCC	3 cut(s)	322, 543, 769
PspPI	GGNCC	2 cut(s)	435, 541
PstNI	CAGNNNCTG	1 cut(s)	645
PsuI	RGATCY	1 cut(s)	119
PsyI	GACNNNGTC	1 cut(s)	911
PvuI	CGATCG	1 cut(s)	831
PvuII	CAGCTG	1 cut(s)	298
RsaI	GTAC	1 cut(s)	203
RsaNI	GTAC	1 cut(s)	202
RseI	CAYNNNNRTG	1 cut(s)	626
SacI	GAGCTC	1 cut(s)	933
SaqAI	TTAA	2 cut(s)	21, 30
SatI	GCNGC	7 cut(s)	267, 270, 342, 361, 500, 582, 796
Sau3AI	GATC	5 cut(s)	93, 119, 760, 787, 828
Sau96I	GGNCC	2 cut(s)	435, 541
SchI	GAGTC	1 cut(s)	480
ScrFI	CCNGG	2 cut(s)	91, 504
SduI	GDGCHC	2 cut(s)	639, 933
SfaNI	GCATC	5 cut(s)	227, 328, 613, 740, 763
SfoI	GGCGCC	1 cut(s)	322
Sfr274I	CTCGAG	1 cut(s)	468
SinI	GGWCC	1 cut(s)	541
SlaI	CTCGAG	1 cut(s)	468
SmiMI	CAYNNNNRTG	1 cut(s)	626
SmlI	CTYRAG	3 cut(s)	353, 468, 608
SmoI	CTYRAG	3 cut(s)	353, 468, 608
SphI	GCATGC	1 cut(s)	459
SsiI	CCGC	9 cut(s)	45, 57, 266, 342, 360, 523, 581, 795, 815
SspDI	GGCGCC	1 cut(s)	320
SspI	AATATT	1 cut(s)	82
SspMI	CTAG	2 cut(s)	75, 884
SstI	GAGCTC	1 cut(s)	933
StyD4I	CCNGG	2 cut(s)	89, 502
StyI	CCWWGG	1 cut(s)	694
TaaI	ACNGT	3 cut(s)	413, 529, 803
TaiI	ACGT	1 cut(s)	925
TaqI	TCGA	4 cut(s)	469, 671, 827, 927
TauI	GCSGC	5 cut(s)	269, 344, 363, 584, 798
TfiI	GAWTC	4 cut(s)	397, 464, 654, 673
Tru1I	TTAA	2 cut(s)	21, 30
Tru9I	TTAA	2 cut(s)	21, 30
TscAI	CASTG	3 cut(s)	244, 706, 806
TseI	GCWGC	2 cut(s)	269, 499
TspDTI	ATGAA	3 cut(s)	355, 606, 697
TspGWI	ACGGA	4 cut(s)	151, 840, 933, 958
TspRI	CASTG	3 cut(s)	244, 706, 806
Tth111I	GACNNNGTC	1 cut(s)	911
Van91I	CCANNNNNTGG	1 cut(s)	683
VpaK11BI	GGWCC	1 cut(s)	541
XceI	RCATGY	2 cut(s)	459, 488
XhoI	CTCGAG	1 cut(s)	468
XmiI	GTMKAC	1 cut(s)	689
XspI	CTAG	2 cut(s)	75, 884

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.