MD10G1022400.v1.1

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000548
Physical Location & Seq
Reverse (-)
2820864 .. 2822754
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1022400.v1.1.491

Sequence Viewer

Length: 807 bp
ATGATCTTTGGTCTAACCATCCACCTTATGAAAATATCATGGGATGATGAAATTAATGATAATCTTGGTTGGCATGGTTGCAGTGAGAAGGAAGAGCTGGACAATGTGGTTGCTGCCAAAATTGAGAAGTTGACGGCTGAACTGCAAAGACCGGATGATCATTTTGACCCAGTTCAAAGGATTGTAGATGGCTTCATCAACTTCAGGATCAACAAATTCGAAAAATACCCAGATTACTACAACGAGCTTGCCAAAGGACAAAACCCCAAGTTTCTGGTATTTGCATGCTCGGACTCCCGAGTGAGTCCCTCACATATCCTTAGTTTCCAACCTGGGGAGGCCTTCATGGTTCGCAACATTGCGAACATGGTTCCTGCATTCAATCAGCTGAAATATGCAGGAGTTGGAGCAGTCATAGAATATGCTATTACAGTACTCGGGGTAACAAATATTTTGGTAATCGGACACAGTCATTGTGGTGGGATAAAGAGGCTTATGACTCACCCTGAGGATAACTCTGTTCCCTTCGACTTCATAGACGAATGGGTCAAAATCGGTTTACCCGCCAAGGCAAAGGTTATAGCAAAGGAAGGGGACGCCGATATTAATGAACAATGTGAGGATTGTGCAAGGGAAGCAGTAAATTTGTCACTGGTAAACCTACAAAGTTACCCTTACGTTCAAAAGGCACTCTCGGACAAAAACCTAGCACTCAGGGGTGGTTACTATGACTTTATTCATGGGATTTTGGAGCTATGGGAGGTTGGGTCGCACATTTCACCTTCCATCATCGTACCAGCACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

269

Amino Acids

30.0

Weight (kDa)

5.17

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 92 - 248 4.3e-40 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 545
AciI CCGC 1 cut(s) 564
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 2 cut(s) 215, 643
AcuI CTGAAG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 2 cut(s) 435, 795
AfiI CCNNNNNNNGG 1 cut(s) 334
AgsI TTSAA 3 cut(s) 176, 382, 683
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 4 cut(s) 97, 247, 388, 754
AluI AGCT 4 cut(s) 97, 247, 388, 754
AlwI GGATC 1 cut(s) 215
Ama87I CYCGRG 2 cut(s) 297, 437
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 2 cut(s) 215, 643
AseI ATTAAT 2 cut(s) 54, 606
AsuHPI GGTGA 2 cut(s) 494, 771
AsuII TTCGAA 1 cut(s) 219
AvaI CYCGRG 2 cut(s) 297, 437
AxyI CCTNAGG 1 cut(s) 507
BbvI GCAGC 1 cut(s) 100
BccI CCATC 3 cut(s) 26, 182, 794
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 333
BclI TGATCA 1 cut(s) 157
BfaI CTAG 1 cut(s) 707
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 1 cut(s) 333
BmeT110I CYCGRG 2 cut(s) 297, 437
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 164
BplI GAGNNNNNCTC 2 cut(s) 500, 532
Bpu14I TTCGAA 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 597
BsaJI CCNNGG 2 cut(s) 332, 567
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 170, 657
Bse21I CCTNAGG 1 cut(s) 507
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 332, 567
BseGI GGATG 3 cut(s) 18, 49, 160
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 498, 727
BseNI ACTGG 2 cut(s) 170, 657
BseXI GCAGC 1 cut(s) 100
BshFI GGCC 1 cut(s) 341
BsiHKCI CYCGRG 2 cut(s) 297, 437
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 291, 608
BslI CCNNNNNNNGG 1 cut(s) 334
BsmFI GGGAC 2 cut(s) 291, 608
BsmI GAATGC 1 cut(s) 377
BsnI GGCC 1 cut(s) 341
BsoBI CYCGRG 2 cut(s) 297, 437
Bsp119I TTCGAA 1 cut(s) 219
Bsp143I GATC 3 cut(s) 3, 157, 207
BspACI CCGC 1 cut(s) 564
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 2 cut(s) 499, 726
BspLI GGNNCC 1 cut(s) 372
BspPI GGATC 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 87
BspT104I TTCGAA 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 2 cut(s) 170, 657
BssECI CCNNGG 2 cut(s) 332, 567
BssMI GATC 3 cut(s) 3, 157, 207
BssNI GRCGYC 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 433, 470
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 597
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 249, 286
BstDEI CTNAG 3 cut(s) 320, 507, 713
BstF5I GGATG 3 cut(s) 18, 49, 160
BstKTI GATC 3 cut(s) 6, 160, 210
BstMBI GATC 3 cut(s) 3, 157, 207
BstMWI GCNNNNNNNGC 1 cut(s) 635
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 100
BstXI CCANNNNNNTGG 1 cut(s) 274
Bsu36I CCTNAGG 1 cut(s) 507
BsuRI GGCC 1 cut(s) 341
BtsCI GGATG 3 cut(s) 18, 49, 160
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 2 cut(s) 88, 650
Cac8I GCNNGC 2 cut(s) 249, 286
CseI GACGC 1 cut(s) 605
Csp6I GTAC 2 cut(s) 434, 794
CviAII CATG 6 cut(s) 39, 74, 285, 346, 367, 740
CviJI RGCY 8 cut(s) 97, 137, 192, 247, 341, 388, 493, 754
CviKI_1 RGCY 8 cut(s) 97, 137, 192, 247, 341, 388, 493, 754
CviQI GTAC 2 cut(s) 434, 794
DdeI CTNAG 3 cut(s) 320, 507, 713
DpnI GATC 3 cut(s) 5, 159, 209
DpnII GATC 3 cut(s) 3, 157, 207
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 567
Eco147I AGGCCT 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 187
Eco81I CCTNAGG 1 cut(s) 507
Eco88I CYCGRG 2 cut(s) 297, 437
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 567
FaeI CATG 6 cut(s) 42, 77, 288, 349, 370, 743
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 2 cut(s) 291, 608
FatI CATG 6 cut(s) 38, 73, 284, 345, 366, 739
FauI CCCGC 1 cut(s) 571
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 3 cut(s) 5, 56, 167
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 1 cut(s) 707
GluI GCNGC 1 cut(s) 114
HaeIII GGCC 1 cut(s) 341
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 605
Hin1I GRCGYC 1 cut(s) 597
Hin1II CATG 6 cut(s) 42, 77, 288, 349, 370, 743
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HinfI GANTC 3 cut(s) 293, 304, 499
HpaII CCGG 1 cut(s) 152
HphI GGTGA 2 cut(s) 494, 771
Hpy166II GTNNAC 3 cut(s) 132, 560, 658
Hpy188I TCNGA 3 cut(s) 292, 464, 697
Hpy188III TCNNGA 2 cut(s) 205, 297
Hpy8I GTNNAC 3 cut(s) 132, 560, 658
HpyAV CCTTC 5 cut(s) 82, 352, 535, 584, 792
HpyCH4III ACNGT 2 cut(s) 433, 470
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 6 cut(s) 81, 145, 284, 377, 398, 629
HpyF10VI GCNNNNNNNGC 1 cut(s) 635
HpyF3I CTNAG 3 cut(s) 320, 507, 713
HpySE526I ACGT 1 cut(s) 678
Hsp92I GRCGYC 1 cut(s) 597
Hsp92II CATG 6 cut(s) 42, 77, 288, 349, 370, 743
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 3, 157, 207
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 2 cut(s) 407, 751
Lsp1109I GCAGC 1 cut(s) 100
MaeI CTAG 1 cut(s) 707
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 4 cut(s) 442, 648, 668, 722
MalI GATC 3 cut(s) 5, 159, 209
MboI GATC 3 cut(s) 3, 157, 207
MboII GAAGA 1 cut(s) 104
MluCI AATT 4 cut(s) 51, 120, 215, 643
MlyI GAGTC 3 cut(s) 287, 313, 493
MmeI TCCRAC 2 cut(s) 352, 385
MnlI CCTC 6 cut(s) 319, 331, 483, 502, 613, 754
MseI TTAA 3 cut(s) 54, 606, 805
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 377
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 635
NdeII GATC 3 cut(s) 3, 157, 207
NlaIII CATG 6 cut(s) 42, 77, 288, 349, 370, 743
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 648
NspI RCATGY 1 cut(s) 288
NspV TTCGAA 1 cut(s) 219
PaeI GCATGC 1 cut(s) 288
PceI AGGCCT 1 cut(s) 341
PciSI GCTCTTC 1 cut(s) 87
PctI GAATGC 1 cut(s) 377
PflFI GACNNNGTC 1 cut(s) 468
PkrI GCNGC 1 cut(s) 115
PleI GAGTC 3 cut(s) 287, 312, 493
PpsI GAGTC 3 cut(s) 287, 312, 493
PshBI ATTAAT 2 cut(s) 54, 606
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 372
PsyI GACNNNGTC 1 cut(s) 468
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 2 cut(s) 435, 795
RsaNI GTAC 2 cut(s) 434, 794
RseI CAYNNNNRTG 1 cut(s) 477
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 3 cut(s) 54, 606, 805
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 3 cut(s) 3, 157, 207
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 3 cut(s) 287, 313, 493
ScrFI CCNGG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 477
SphI GCATGC 1 cut(s) 288
Sse9I AATT 4 cut(s) 51, 120, 215, 643
SseBI AGGCCT 1 cut(s) 341
SsiI CCGC 1 cut(s) 564
SspI AATATT 1 cut(s) 451
SspMI CTAG 1 cut(s) 707
StuI AGGCCT 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 2 cut(s) 433, 470
TaiI ACGT 1 cut(s) 681
TaqI TCGA 2 cut(s) 219, 528
TasI AATT 4 cut(s) 51, 120, 215, 643
TatI WGTACW 1 cut(s) 433
Tru1I TTAA 3 cut(s) 54, 606, 805
Tru9I TTAA 3 cut(s) 54, 606, 805
TscAI CASTG 2 cut(s) 88, 657
TseFI GTSAC 1 cut(s) 648
TseI GCWGC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 648
TspDTI ATGAA 7 cut(s) 44, 63, 184, 334, 523, 624, 728
TspRI CASTG 2 cut(s) 88, 657
Tth111I GACNNNGTC 1 cut(s) 468
VspI ATTAAT 2 cut(s) 54, 606
XapI RAATTY 2 cut(s) 215, 643
XceI RCATGY 1 cut(s) 288
XspI CTAG 1 cut(s) 707
ZrmI AGTACT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.