Rroxscaffold_1G00016930

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000548
Physical Location & Seq
Forward (+)
21045335 .. 21052190
6856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00016930.1

Sequence Viewer

Length: 1299 bp
ATGACTAGCGTGTTATCTGATCTGATCAATACTGAAGATCTCATCAAGACTGATCTGATCAGCACTGAGAATGAAAACCAGGATGATGTGGTTGCTACTGAAACTGAGAGCCTGGAAGATGACCCAGTTCAAACAATTTTAGACGGGTTCAAGCACTTCAAGACCAACTTATACGACACAAATCCAGAGTTGTTCAATGAGCTTGCCCTAGCACAAAGCCCCAAGTTTTTGGTATTTGCATGCTCGGATTCCCGAGTGAGTCCCTCGCATGTTCTTCATTGCATACCTGGACAAGCCTTTCTGGTTCGCAACATTGCTAACATGGTTCCTGCATATGACCAGCTAAGGTACACAGGAACTGGAGCAACCATTGAGTATGCTGTAGAAGAACTCGGGGTAGAAAACATTTTGATAGTGGGACACAGTAGTTGCGGAGGAATAAAAAGGCTAATGTCTTATCCCGAGGATGGTTCCGCTCCACTGGTCTTTATAGATGATTGGATGAAAATGGCTTTACCTGTCAAGACAAAGGTTATTGCGGAAGCTGGCGATACCGATTTCCAGGAACAATGTGATCGTTGCGCAAGGTTATCTGATTTGACCAATATTCAAGATCTCATCTTGAAAAAGCTTGTGAGTTATCAGAAGGAAGACCTGGAAGATGTAGCTGCTCCCAAACTTCAGAACCTCACGGTTGAGTGCAATGACCTGAAAGGAGCTGAACCAAATGCACCGACCAGCCCAATCGCCAGCCCTCTCGACCCAGTTAAAAGAATTATAGATGGCTTCAAGCACTTCAAGACCAGCTACTTCGATTTATGGTATTTGCATGCTCGGATTCTCGAGTGTACGTGTCCTTCACATGTTCTTCACTTCCAACCAGGGGAGGCCTTCATCGTTCGCAACATTGCTAACATGGTTCCTGCTTTTGATCAGGTGGCAAATATTTTGGTAATGGGACACAATAATTGTGGTGGAATAAAGAGGCTAATGTCTTATCCGGAGGATGGCTCTGAGCCCTTCGACTTCATAGATGAATGGGTGAAAATGAGTTTACTAGCCAAGGCTGAGGTTATTGCAGAAGCTGAGAGTGCAGATTTCCATGAACAATGTGAAAGATGTGCAAGGGAATCAGTAAACTTGTCACTGGCAAACCTACTTACCTACCCCTTTGTTCAAAAGGCACACTTGGATAAAAAACTAGCACTTCGGGGTGGATACTATGACTTTGTCAATGGAATTTTCGAGCTATGGGAGCTCAAATCCCACATTTCAAACCCCATCATCGTACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

432

Amino Acids

48.28

Weight (kDa)

4.73

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 77 - 213 1e-30 Carbonic anhydrase
Pro_CA PF00484 286 - 413 3.8e-21 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 583
AccBSI CCGCTC 1 cut(s) 476
AccIII TCCGGA 1 cut(s) 1000
AciI CCGC 3 cut(s) 432, 474, 539
AcsI RAATTY 1 cut(s) 1239
AcuI CTGAAG 2 cut(s) 54, 665
AfaI GTAC 3 cut(s) 350, 850, 1290
AfiI CCNNNNNNNGG 3 cut(s) 467, 883, 1007
AflIII ACRYGT 2 cut(s) 851, 862
AjnI CCWGG 6 cut(s) 78, 111, 286, 561, 654, 880
Alw21I GWGCWC 1 cut(s) 1260
AlwNI CAGNNNCTG 2 cut(s) 359, 1085
Ama87I CYCGRG 4 cut(s) 252, 392, 461, 842
Aor13HI TCCGGA 1 cut(s) 1000
AoxI GGCC 1 cut(s) 888
ApeKI GCWGC 1 cut(s) 668
ApoI RAATTY 1 cut(s) 1239
AspLEI GCGC 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 1054
AvaI CYCGRG 4 cut(s) 252, 392, 461, 842
BanII GRGCYC 2 cut(s) 1020, 1260
BarI GAAGNNNNNNTAC 2 cut(s) 841, 873
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 1260
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 1 cut(s) 655
BccI CCATC 4 cut(s) 461, 776, 1001, 1289
BciT130I CCWGG 6 cut(s) 80, 113, 288, 563, 656, 882
BciVI GTATCC 1 cut(s) 1211
BclI TGATCA 3 cut(s) 24, 57, 931
BfaI CTAG 4 cut(s) 6, 209, 1058, 1202
BfmI CTRYAG 1 cut(s) 381
BfuI GTATCC 1 cut(s) 1211
BglII AGATCT 2 cut(s) 37, 613
BisI GCNGC 1 cut(s) 669
BlsI GCNGC 1 cut(s) 670
Bme1390I CCNGG 6 cut(s) 80, 113, 288, 563, 656, 882
BmeT110I CYCGRG 4 cut(s) 252, 392, 461, 842
BmiI GGNNCC 3 cut(s) 327, 472, 921
BmrFI CCNGG 6 cut(s) 80, 113, 288, 563, 656, 882
BmrI ACTGGG 2 cut(s) 119, 758
BmuI ACTGGG 2 cut(s) 119, 758
BpiI GAAGAC 1 cut(s) 657
BplI GAGNNNNNCTC 2 cut(s) 995, 1027
BpmI CTGGAG 1 cut(s) 381
Bpu10I CCTNAGC 2 cut(s) 344, 1068
BsaAI YACGTR 1 cut(s) 852
BsaJI CCNNGG 3 cut(s) 462, 881, 1062
BsaWI WCCGGW 1 cut(s) 1000
Bsc4I CCNNNNNNNGG 3 cut(s) 467, 883, 1007
Bse1I ACTGG 5 cut(s) 125, 364, 486, 764, 1152
Bse3DI GCAATG 4 cut(s) 277, 312, 709, 906
BseAI TCCGGA 1 cut(s) 1000
BseBI CCWGG 6 cut(s) 80, 113, 288, 563, 656, 882
BseDI CCNNGG 3 cut(s) 462, 881, 1062
BseGI GGATG 4 cut(s) 88, 472, 507, 1012
BseLI CCNNNNNNNGG 3 cut(s) 467, 883, 1007
BseMI GCAATG 4 cut(s) 277, 312, 709, 906
BseMII CTCAG 5 cut(s) 57, 96, 1005, 1059, 1077
BseNI ACTGG 5 cut(s) 125, 364, 486, 764, 1152
BseXI GCAGC 1 cut(s) 655
BsgI GTGCAG 1 cut(s) 1113
BshFI GGCC 1 cut(s) 890
BsiHKAI GWGCWC 1 cut(s) 1260
BsiHKCI CYCGRG 4 cut(s) 252, 392, 461, 842
BsiSI CCGG 1 cut(s) 1001
BslFI GGGAC 3 cut(s) 246, 432, 972
BslI CCNNNNNNNGG 3 cut(s) 467, 883, 1007
BsmFI GGGAC 3 cut(s) 246, 432, 972
BsnI GGCC 1 cut(s) 890
BsoBI CYCGRG 4 cut(s) 252, 392, 461, 842
Bsp1286I GDGCHC 2 cut(s) 1020, 1260
Bsp13I TCCGGA 1 cut(s) 1000
Bsp143I GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
BspACI CCGC 3 cut(s) 432, 474, 539
BspANI GGCC 1 cut(s) 890
BspCNI CTCAG 5 cut(s) 58, 97, 1006, 1060, 1078
BspEI TCCGGA 1 cut(s) 1000
BspLI GGNNCC 3 cut(s) 327, 472, 921
BsrBI CCGCTC 1 cut(s) 476
BsrDI GCAATG 4 cut(s) 277, 312, 709, 906
BsrI ACTGG 5 cut(s) 125, 364, 486, 764, 1152
BssECI CCNNGG 3 cut(s) 462, 881, 1062
BssMI GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
BssT1I CCWWGG 1 cut(s) 1062
Bst2UI CCWGG 6 cut(s) 80, 113, 288, 563, 656, 882
Bst4CI ACNGT 2 cut(s) 425, 694
BstBAI YACGTR 1 cut(s) 852
BstC8I GCNNGC 5 cut(s) 204, 241, 547, 751, 831
BstDEI CTNAG 6 cut(s) 66, 105, 344, 1014, 1068, 1086
BstF5I GGATG 4 cut(s) 88, 472, 507, 1012
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 8 cut(s) 22, 27, 40, 55, 60, 577, 616, 934
BstMBI GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
BstMWI GCNNNNNNNGC 2 cut(s) 1091, 1255
BstNI CCWGG 6 cut(s) 80, 113, 288, 563, 656, 882
BstNSI RCATGY 4 cut(s) 243, 272, 833, 866
BstSCI CCNGG 6 cut(s) 78, 111, 286, 561, 654, 880
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 655
BstV2I GAAGAC 1 cut(s) 657
BstX2I RGATCY 2 cut(s) 37, 613
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 2 cut(s) 37, 613
BsuI GTATCC 1 cut(s) 1211
BsuRI GGCC 1 cut(s) 890
BtsCI GGATG 4 cut(s) 88, 472, 507, 1012
BtsIMutI CAGTG 3 cut(s) 63, 479, 1145
Cac8I GCNNGC 5 cut(s) 204, 241, 547, 751, 831
CaiI CAGNNNCTG 2 cut(s) 359, 1085
CfoI GCGC 1 cut(s) 584
Csp6I GTAC 3 cut(s) 349, 849, 1289
CspCI CAANNNNNGTGG 2 cut(s) 952, 987
CviAII CATG 7 cut(s) 240, 269, 322, 830, 863, 916, 1103
CviQI GTAC 3 cut(s) 349, 849, 1289
DdeI CTNAG 6 cut(s) 66, 105, 344, 1014, 1068, 1086
DpnI GATC 8 cut(s) 21, 26, 39, 54, 59, 576, 615, 933
DpnII GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
Ecl136II GAGCTC 1 cut(s) 1258
Eco130I CCWWGG 1 cut(s) 1062
Eco147I AGGCCT 1 cut(s) 890
Eco24I GRGCYC 2 cut(s) 1020, 1260
Eco53kI GAGCTC 1 cut(s) 1258
Eco57I CTGAAG 2 cut(s) 54, 665
Eco88I CYCGRG 4 cut(s) 252, 392, 461, 842
EcoICRI GAGCTC 1 cut(s) 1258
EcoRII CCWGG 6 cut(s) 78, 111, 286, 561, 654, 880
EcoT14I CCWWGG 1 cut(s) 1062
EcoT38I GRGCYC 2 cut(s) 1020, 1260
ErhI CCWWGG 1 cut(s) 1062
FaeI CATG 7 cut(s) 243, 272, 325, 833, 866, 919, 1106
FalI AAGNNNNNCTT 4 cut(s) 152, 184, 1172, 1204
FaqI GGGAC 3 cut(s) 246, 432, 972
FatI CATG 7 cut(s) 239, 268, 321, 829, 862, 915, 1102
FauNDI CATATG 1 cut(s) 334
FbaI TGATCA 3 cut(s) 24, 57, 931
Fnu4HI GCNGC 1 cut(s) 669
FokI GGATG 4 cut(s) 95, 479, 514, 1019
FriOI GRGCYC 2 cut(s) 1020, 1260
Fsp4HI GCNGC 1 cut(s) 669
FspBI CTAG 4 cut(s) 6, 209, 1058, 1202
FspI TGCGCA 1 cut(s) 583
GlaI GCGC 1 cut(s) 583
GluI GCNGC 1 cut(s) 669
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 890
HapII CCGG 1 cut(s) 1001
HhaI GCGC 1 cut(s) 584
Hin1II CATG 7 cut(s) 243, 272, 325, 833, 866, 919, 1106
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 4 cut(s) 248, 259, 838, 1130
HpaII CCGG 1 cut(s) 1001
HphI GGTGA 1 cut(s) 1054
Hpy166II GTNNAC 4 cut(s) 351, 849, 1055, 1138
Hpy188I TCNGA 9 cut(s) 19, 24, 57, 247, 595, 645, 684, 837, 1015
Hpy8I GTNNAC 4 cut(s) 351, 849, 1055, 1138
HpyAV CCTTC 4 cut(s) 640, 867, 901, 1030
HpyCH4III ACNGT 2 cut(s) 425, 694
HpyCH4IV ACGT 1 cut(s) 851
HpyCH4V TGCA 9 cut(s) 239, 282, 332, 702, 731, 829, 1079, 1094, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 1091, 1255
HpyF3I CTNAG 6 cut(s) 66, 105, 344, 1014, 1068, 1086
HpySE526I ACGT 1 cut(s) 851
Hsp92II CATG 7 cut(s) 243, 272, 325, 833, 866, 919, 1106
HspAI GCGC 1 cut(s) 582
Kpn2I TCCGGA 1 cut(s) 1000
Ksp22I TGATCA 3 cut(s) 24, 57, 931
Kzo9I GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
LmnI GCTCC 5 cut(s) 362, 481, 676, 716, 1255
Lsp1109I GCAGC 1 cut(s) 655
MaeI CTAG 4 cut(s) 6, 209, 1058, 1202
MaeII ACGT 1 cut(s) 851
MaeIII GTNAC 1 cut(s) 1143
MalI GATC 8 cut(s) 21, 26, 39, 54, 59, 576, 615, 933
MbiI CCGCTC 1 cut(s) 476
MboI GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
MboII GAAGA 7 cut(s) 47, 128, 266, 398, 662, 671, 860
MflI RGATCY 2 cut(s) 37, 613
MhlI GDGCHC 2 cut(s) 1020, 1260
MluCI AATT 4 cut(s) 135, 774, 967, 1239
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 901
MnlI CCTC 9 cut(s) 274, 428, 457, 698, 765, 880, 978, 997, 1063
MroI TCCGGA 1 cut(s) 1000
MseI TTAA 1 cut(s) 768
MspI CCGG 1 cut(s) 1001
MspR9I CCNGG 6 cut(s) 80, 113, 288, 563, 656, 882
MvaI CCWGG 6 cut(s) 80, 113, 288, 563, 656, 882
MwoI GCNNNNNNNGC 2 cut(s) 1091, 1255
NdeI CATATG 1 cut(s) 334
NdeII GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
NlaIII CATG 7 cut(s) 243, 272, 325, 833, 866, 919, 1106
NlaIV GGNNCC 3 cut(s) 327, 472, 921
NmuCI GTSAC 1 cut(s) 1143
NsbI TGCGCA 1 cut(s) 583
NspI RCATGY 4 cut(s) 243, 272, 833, 866
PaeI GCATGC 2 cut(s) 243, 833
PaeR7I CTCGAG 1 cut(s) 842
PceI AGGCCT 1 cut(s) 890
PciI ACATGT 1 cut(s) 862
PfeI GAWTC 3 cut(s) 248, 838, 1130
PflFI GACNNNGTC 1 cut(s) 1229
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 1 cut(s) 670
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
Ppu21I YACGTR 1 cut(s) 852
PscI ACATGT 1 cut(s) 862
Psp124BI GAGCTC 1 cut(s) 1260
Psp6I CCWGG 6 cut(s) 78, 111, 286, 561, 654, 880
PspGI CCWGG 6 cut(s) 78, 111, 286, 561, 654, 880
PspN4I GGNNCC 3 cut(s) 327, 472, 921
PstNI CAGNNNCTG 2 cut(s) 359, 1085
PsuI RGATCY 2 cut(s) 37, 613
PsyI GACNNNGTC 1 cut(s) 1229
RsaI GTAC 3 cut(s) 350, 850, 1290
RsaNI GTAC 3 cut(s) 349, 849, 1289
SacI GAGCTC 1 cut(s) 1260
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 1 cut(s) 669
Sau3AI GATC 8 cut(s) 19, 24, 37, 52, 57, 574, 613, 931
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 6 cut(s) 80, 113, 288, 563, 656, 882
SduI GDGCHC 2 cut(s) 1020, 1260
SfcI CTRYAG 1 cut(s) 381
Sfr274I CTCGAG 1 cut(s) 842
SlaI CTCGAG 1 cut(s) 842
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
SphI GCATGC 2 cut(s) 243, 833
Sse9I AATT 4 cut(s) 135, 774, 967, 1239
SseBI AGGCCT 1 cut(s) 890
SsiI CCGC 3 cut(s) 432, 474, 539
SspI AATATT 2 cut(s) 607, 946
SspMI CTAG 4 cut(s) 6, 209, 1058, 1202
SstI GAGCTC 1 cut(s) 1260
StuI AGGCCT 1 cut(s) 890
StyD4I CCNGG 6 cut(s) 78, 111, 286, 561, 654, 880
StyI CCWWGG 1 cut(s) 1062
TaaI ACNGT 2 cut(s) 425, 694
TaiI ACGT 1 cut(s) 854
TaqI TCGA 5 cut(s) 759, 813, 843, 1023, 1245
TasI AATT 4 cut(s) 135, 774, 967, 1239
TfiI GAWTC 3 cut(s) 248, 838, 1130
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 3 cut(s) 70, 486, 1152
TseFI GTSAC 1 cut(s) 1143
TseI GCWGC 1 cut(s) 668
Tsp45I GTSAC 1 cut(s) 1143
TspDTI ATGAA 7 cut(s) 87, 266, 518, 883, 1018, 1050, 1119
TspRI CASTG 3 cut(s) 70, 486, 1152
Tth111I GACNNNGTC 1 cut(s) 1229
XapI RAATTY 1 cut(s) 1239
XceI RCATGY 4 cut(s) 243, 272, 833, 866
XhoI CTCGAG 1 cut(s) 842
XspI CTAG 4 cut(s) 6, 209, 1058, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.