Rh5DG447900

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000548
Physical Location & Seq
Reverse (-)
71451551 .. 71453336
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG447900.1

Sequence Viewer

Length: 822 bp
ATGACTAGCCAGTTATCTGATTTGACCAACATTCAAGATCTCATCTTGAAAAAGCTTGTGAGTTATCAGAAGGAAGACCTGGAAGATGTAGCTGCTCCCAAACTTCAGAACCTCAAGGTTGAGTGCAATGACCTGAAAGGAGCTGAACCAAATACACCGACCAGCCCAATAGCCAGCCCTCTCGACCCAGTTAAAAGAATTATAGATGGCTTCAAGTACTTCAAGACCAGCTACTTCGACACAAAACCAGCTTTGTTTGATGAGCTTGCCCAAGGACAAAGCCCCGAGTTTATGGTATTTGCATGCTCGGATTCTCGAGTGTGTCCTTCACATGTTCTTCACTTCCAACCAGGGGAGGCCTTCATCGTTCGCAACATTGCTAACATGGTTCCTGCTTTTGATCAGCTAAAACATGCAGGAGTTGGAGCAACCATAGAATATGCTGTCAAAGAACTCGGGGTGGCAAATATTTTGGTAATGGGACACAGTAATTGTGGTGGAATAAAGAGGCTAATGTCTTATCCGGAGGATGGCTCTAAACCCTTCGACTTCATAGATGAATGGGTGAAAATGATTTTACCAGCCAAGGCTGAGGTTATTGCAGAAGCTGGGAGTGCAGATTTCCACGAACAATGTGAAAGATGTGCAAGGGAATCAGTAAACTTGTCACTGGCAAACCTACTTACCTACCCCTTTGTTCAAAAAGCACACTTGGATGAAAAACTAGCACTTCGGGGTGGATACCATGACTTTGTCAATGGAATTTTCGAGCTATGGGAGCTCAAATCCCAAATTTTAAACCCCATCATCGTACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

273

Amino Acids

30.35

Weight (kDa)

5.2

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 98 - 254 2.1e-41 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 523
AcsI RAATTY 2 cut(s) 762, 792
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 2 cut(s) 218, 813
AfiI CCNNNNNNNGG 2 cut(s) 352, 530
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 5 cut(s) 35, 49, 214, 223, 701
AjnI CCWGG 2 cut(s) 78, 349
Alw21I GWGCWC 1 cut(s) 783
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 3 cut(s) 284, 315, 455
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 2 cut(s) 762, 792
AsuHPI GGTGA 1 cut(s) 577
AvaI CYCGRG 3 cut(s) 284, 315, 455
BanII GRGCYC 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 783
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 79
BccI CCATC 3 cut(s) 200, 524, 812
BciT130I CCWGG 2 cut(s) 80, 351
BciVI GTATCC 1 cut(s) 734
BclI TGATCA 1 cut(s) 400
BfaI CTAG 2 cut(s) 6, 725
BfuI GTATCC 1 cut(s) 734
BglII AGATCT 1 cut(s) 37
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 80, 351
BmeT110I CYCGRG 3 cut(s) 284, 315, 455
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 80, 351
BmrI ACTGGG 1 cut(s) 182
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 518, 550
Bpu10I CCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 271, 350, 585
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 530
Bse1I ACTGG 3 cut(s) 10, 188, 675
Bse3DI GCAATG 2 cut(s) 133, 375
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 2 cut(s) 80, 351
BseDI CCNNGG 3 cut(s) 271, 350, 585
BseGI GGATG 2 cut(s) 535, 721
BseLI CCNNNNNNNGG 2 cut(s) 352, 530
BseMI GCAATG 2 cut(s) 133, 375
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 3 cut(s) 10, 188, 675
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 783
BsiHKCI CYCGRG 3 cut(s) 284, 315, 455
BsiSI CCGG 1 cut(s) 524
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 2 cut(s) 352, 530
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 3 cut(s) 284, 315, 455
Bsp1286I GDGCHC 1 cut(s) 783
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 2 cut(s) 37, 400
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 1 cut(s) 583
BspEI TCCGGA 1 cut(s) 523
BspLI GGNNCC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 133, 375
BsrI ACTGG 3 cut(s) 10, 188, 675
BssECI CCNNGG 3 cut(s) 271, 350, 585
BssMI GATC 2 cut(s) 37, 400
BssT1I CCWWGG 2 cut(s) 271, 585
Bst2UI CCWGG 2 cut(s) 80, 351
Bst4CI ACNGT 1 cut(s) 488
BstC8I GCNNGC 3 cut(s) 175, 267, 304
BstDEI CTNAG 1 cut(s) 591
BstF5I GGATG 2 cut(s) 535, 721
BstKTI GATC 2 cut(s) 40, 403
BstMBI GATC 2 cut(s) 37, 400
BstMWI GCNNNNNNNGC 2 cut(s) 614, 778
BstNI CCWGG 2 cut(s) 80, 351
BstNSI RCATGY 3 cut(s) 306, 335, 416
BstSCI CCNGG 2 cut(s) 78, 349
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 1 cut(s) 359
BtsCI GGATG 2 cut(s) 535, 721
BtsIMutI CAGTG 1 cut(s) 668
Cac8I GCNNGC 3 cut(s) 175, 267, 304
CaiI CAGNNNCTG 1 cut(s) 608
Csp6I GTAC 2 cut(s) 217, 812
CviAII CATG 5 cut(s) 303, 332, 385, 413, 746
CviQI GTAC 2 cut(s) 217, 812
DdeI CTNAG 1 cut(s) 591
DpnI GATC 2 cut(s) 39, 402
DpnII GATC 2 cut(s) 37, 400
DraI TTTAAA 1 cut(s) 798
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 2 cut(s) 271, 585
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 783
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 3 cut(s) 284, 315, 455
EcoICRI GAGCTC 1 cut(s) 781
EcoRII CCWGG 2 cut(s) 78, 349
EcoT14I CCWWGG 2 cut(s) 271, 585
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 2 cut(s) 271, 585
FaeI CATG 5 cut(s) 306, 335, 388, 416, 749
FaqI GGGAC 1 cut(s) 495
FatI CATG 5 cut(s) 302, 331, 384, 412, 745
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 2 cut(s) 542, 728
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 2 cut(s) 6, 725
GluI GCNGC 1 cut(s) 93
GsaI CCCAGC 1 cut(s) 612
HaeIII GGCC 1 cut(s) 359
HapII CCGG 1 cut(s) 524
Hin1II CATG 5 cut(s) 306, 335, 388, 416, 749
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 311, 653
HpaII CCGG 1 cut(s) 524
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 4 cut(s) 19, 69, 108, 310
Hpy188III TCNNGA 7 cut(s) 35, 46, 182, 223, 315, 524, 819
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 4 cut(s) 64, 336, 370, 553
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4V TGCA 6 cut(s) 126, 302, 416, 602, 617, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 614, 778
HpyF3I CTNAG 1 cut(s) 591
Hsp92II CATG 5 cut(s) 306, 335, 388, 416, 749
Kpn2I TCCGGA 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 2 cut(s) 37, 400
LmnI GCTCC 4 cut(s) 100, 140, 425, 778
Lsp1109I GCAGC 1 cut(s) 79
MaeI CTAG 2 cut(s) 6, 725
MaeIII GTNAC 1 cut(s) 666
MalI GATC 2 cut(s) 39, 402
MboI GATC 2 cut(s) 37, 400
MboII GAAGA 3 cut(s) 86, 95, 329
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 783
MluCI AATT 4 cut(s) 198, 490, 762, 792
MmeI TCCRAC 2 cut(s) 370, 403
MnlI CCTC 6 cut(s) 122, 189, 349, 501, 520, 586
MroI TCCGGA 1 cut(s) 523
MseI TTAA 2 cut(s) 192, 797
MslI CAYNNNNRTG 1 cut(s) 714
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 2 cut(s) 80, 351
MvaI CCWGG 2 cut(s) 80, 351
MwoI GCNNNNNNNGC 2 cut(s) 614, 778
NdeII GATC 2 cut(s) 37, 400
NlaIII CATG 5 cut(s) 306, 335, 388, 416, 749
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 666
NspI RCATGY 3 cut(s) 306, 335, 416
PaeI GCATGC 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 315
PceI AGGCCT 1 cut(s) 359
PciI ACATGT 1 cut(s) 331
PfeI GAWTC 2 cut(s) 311, 653
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 1 cut(s) 94
PscI ACATGT 1 cut(s) 331
Psp124BI GAGCTC 1 cut(s) 783
Psp6I CCWGG 2 cut(s) 78, 349
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 78, 349
PspN4I GGNNCC 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 752
RsaI GTAC 2 cut(s) 218, 813
RsaNI GTAC 2 cut(s) 217, 812
RseI CAYNNNNRTG 1 cut(s) 714
SacI GAGCTC 1 cut(s) 783
SaqAI TTAA 2 cut(s) 192, 797
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 2 cut(s) 37, 400
ScaI AGTACT 1 cut(s) 218
ScrFI CCNGG 2 cut(s) 80, 351
SduI GDGCHC 1 cut(s) 783
Sfr274I CTCGAG 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 2 cut(s) 113, 315
SmoI CTYRAG 2 cut(s) 113, 315
SphI GCATGC 1 cut(s) 306
Sse9I AATT 4 cut(s) 198, 490, 762, 792
SseBI AGGCCT 1 cut(s) 359
SspI AATATT 1 cut(s) 469
SspMI CTAG 2 cut(s) 6, 725
SstI GAGCTC 1 cut(s) 783
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 78, 349
StyI CCWWGG 2 cut(s) 271, 585
TaaI ACNGT 1 cut(s) 488
TaqI TCGA 5 cut(s) 183, 237, 316, 546, 768
TasI AATT 4 cut(s) 198, 490, 762, 792
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 2 cut(s) 311, 653
Tru1I TTAA 2 cut(s) 192, 797
Tru9I TTAA 2 cut(s) 192, 797
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 1 cut(s) 666
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 666
TspDTI ATGAA 4 cut(s) 352, 541, 573, 732
TspRI CASTG 1 cut(s) 675
Tth111I GACNNNGTC 1 cut(s) 752
XapI RAATTY 2 cut(s) 762, 792
XceI RCATGY 3 cut(s) 306, 335, 416
XhoI CTCGAG 1 cut(s) 315
XspI CTAG 2 cut(s) 6, 725
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.