RLG00000035683

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000548
Physical Location & Seq
Reverse (-)
70218008 .. 70219781
1774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035683

Sequence Viewer

Length: 822 bp
ATGACTAGCCAGTTATCTGATTTGACCAATATTCAAGATCTCATCTTGAAAAAGCTTGTGAGTTATCAGAAGGAAGACCCGGAAGATCTAGCTGCTCCCAAACTTCGGAACCTCACGGTTGAGTGCAATGACCTGAAAGGAGCTGAACCAAATGCACCGACCAGCCCAATCGCCAGTCCTCTCGACCCAGTTAAAAGAATTATAGATGGCTTCAAGCACTTCAAGACCAGCTACTTCGACACAAATCCAGCTTTGTTTGATGAGCTTGCCCAAGGACAAAACCCCGAGTTTATGGTATTTGCATGCTCGGATTCTCGAGTGTGTCCTTCACATGTTCTTCACTTCCAACCTGGGGAGGCCTTCATCGTTCGCAACATTGCTAACATGGTTCCTGCTTTTGATCAGCTAAAACATGCAGGAGTTGGAGCAACCATAGAATATGCTGTCAAAGAACTCGGGGTGGCAAATATTTTGGTAATGGGACACAGTAATTGTGGTGGGATAAAGAGGCTAATGTCTTATCCGGAGGATGGCTCTGAGCCCTTCGACTTCATAGATGAATGGGTGAAAATGAGTTTACCAGCCAAGGCTGAAGTTATTGCTGAAGCTGGGAGTGCAGATTTCCATGAACAATGTGAAAGATGTGCAAGGGAATCAGTAAACTTGTCACTGGCAAACCTACTTACCTACCCCTTTGTTCAAAAGGCACACTTGGATAAAAAACTAGCACTTCGGGGTGGGTACTATGACTTTGTCAATGGAATTTTCGAGCTATGGGAGCTCAAATCCCACATTTCAAACCCTATCATCGTACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

274

Amino Acids

30.29

Weight (kDa)

5.19

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 98 - 254 1e-41 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 523
AcsI RAATTY 1 cut(s) 762
AcuI CTGAAG 2 cut(s) 612, 624
AfaI GTAC 2 cut(s) 743, 813
AfiI CCNNNNNNNGG 3 cut(s) 105, 352, 530
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 6 cut(s) 35, 49, 214, 223, 701, 798
AjnI CCWGG 1 cut(s) 349
Alw21I GWGCWC 1 cut(s) 783
Ama87I CYCGRG 3 cut(s) 284, 315, 455
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 762
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 577
AvaI CYCGRG 3 cut(s) 284, 315, 455
BanII GRGCYC 2 cut(s) 543, 783
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 200, 524
BciT130I CCWGG 1 cut(s) 351
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 1 cut(s) 80
BfaI CTAG 3 cut(s) 6, 89, 725
BglII AGATCT 2 cut(s) 37, 85
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 80, 351
BmeT110I CYCGRG 3 cut(s) 284, 315, 455
BmiI GGNNCC 2 cut(s) 110, 390
BmrFI CCNGG 2 cut(s) 80, 351
BmrI ACTGGG 1 cut(s) 182
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 271, 350, 585
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 352, 530
Bse1I ACTGG 4 cut(s) 10, 174, 188, 675
Bse3DI GCAATG 2 cut(s) 133, 375
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 271, 350, 585
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 3 cut(s) 105, 352, 530
BseMI GCAATG 2 cut(s) 133, 375
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 4 cut(s) 10, 174, 188, 675
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 783
BsiHKCI CYCGRG 3 cut(s) 284, 315, 455
BsiSI CCGG 2 cut(s) 80, 524
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 3 cut(s) 105, 352, 530
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 3 cut(s) 284, 315, 455
Bsp1286I GDGCHC 2 cut(s) 543, 783
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 3 cut(s) 37, 85, 400
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 1 cut(s) 529
BspEI TCCGGA 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 110, 390
BsrDI GCAATG 2 cut(s) 133, 375
BsrI ACTGG 4 cut(s) 10, 174, 188, 675
BssECI CCNNGG 3 cut(s) 271, 350, 585
BssMI GATC 3 cut(s) 37, 85, 400
BssT1I CCWWGG 2 cut(s) 271, 585
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 118, 488
BstC8I GCNNGC 2 cut(s) 267, 304
BstDEI CTNAG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 3 cut(s) 40, 88, 403
BstMBI GATC 3 cut(s) 37, 85, 400
BstMWI GCNNNNNNNGC 2 cut(s) 614, 778
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 3 cut(s) 306, 335, 416
BstSCI CCNGG 2 cut(s) 78, 349
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 2 cut(s) 37, 85
BstYI RGATCY 2 cut(s) 37, 85
BsuRI GGCC 1 cut(s) 359
BtsCI GGATG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 668
Cac8I GCNNGC 2 cut(s) 267, 304
Csp6I GTAC 2 cut(s) 742, 812
CviAII CATG 5 cut(s) 303, 332, 385, 413, 626
CviQI GTAC 2 cut(s) 742, 812
DdeI CTNAG 1 cut(s) 537
DpnI GATC 3 cut(s) 39, 87, 402
DpnII GATC 3 cut(s) 37, 85, 400
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 2 cut(s) 271, 585
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 2 cut(s) 543, 783
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 2 cut(s) 612, 624
Eco88I CYCGRG 3 cut(s) 284, 315, 455
EcoICRI GAGCTC 1 cut(s) 781
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 2 cut(s) 271, 585
EcoT38I GRGCYC 2 cut(s) 543, 783
ErhI CCWWGG 2 cut(s) 271, 585
FaeI CATG 5 cut(s) 306, 335, 388, 416, 629
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 1 cut(s) 495
FatI CATG 5 cut(s) 302, 331, 384, 412, 625
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 2 cut(s) 543, 783
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 3 cut(s) 6, 89, 725
GluI GCNGC 1 cut(s) 93
GsaI CCCAGC 1 cut(s) 612
HaeIII GGCC 1 cut(s) 359
HapII CCGG 2 cut(s) 80, 524
Hin1II CATG 5 cut(s) 306, 335, 388, 416, 629
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 311, 653
HpaII CCGG 2 cut(s) 80, 524
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 2 cut(s) 578, 661
Hpy188I TCNGA 5 cut(s) 19, 69, 108, 310, 538
Hpy188III TCNNGA 7 cut(s) 35, 46, 182, 223, 315, 524, 819
Hpy8I GTNNAC 2 cut(s) 578, 661
HpyAV CCTTC 4 cut(s) 64, 336, 370, 553
HpyCH4III ACNGT 2 cut(s) 118, 488
HpyCH4V TGCA 6 cut(s) 126, 155, 302, 416, 617, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 614, 778
HpyF3I CTNAG 1 cut(s) 537
Hsp92II CATG 5 cut(s) 306, 335, 388, 416, 629
Kpn2I TCCGGA 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 3 cut(s) 37, 85, 400
LmnI GCTCC 4 cut(s) 100, 140, 425, 778
Lsp1109I GCAGC 1 cut(s) 79
MaeI CTAG 3 cut(s) 6, 89, 725
MaeIII GTNAC 1 cut(s) 666
MalI GATC 3 cut(s) 39, 87, 402
MboI GATC 3 cut(s) 37, 85, 400
MboII GAAGA 3 cut(s) 86, 95, 329
MflI RGATCY 2 cut(s) 37, 85
MhlI GDGCHC 2 cut(s) 543, 783
MluCI AATT 3 cut(s) 198, 490, 762
MmeI TCCRAC 2 cut(s) 370, 403
MnlI CCTC 5 cut(s) 122, 189, 349, 501, 520
MroI TCCGGA 1 cut(s) 523
MseI TTAA 1 cut(s) 192
MspI CCGG 2 cut(s) 80, 524
MspR9I CCNGG 2 cut(s) 80, 351
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 614, 778
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 37, 85, 400
NlaIII CATG 5 cut(s) 306, 335, 388, 416, 629
NlaIV GGNNCC 2 cut(s) 110, 390
NmuCI GTSAC 1 cut(s) 666
NspI RCATGY 3 cut(s) 306, 335, 416
PaeI GCATGC 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 315
PceI AGGCCT 1 cut(s) 359
PciI ACATGT 1 cut(s) 331
PfeI GAWTC 2 cut(s) 311, 653
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 1 cut(s) 94
PscI ACATGT 1 cut(s) 331
Psp124BI GAGCTC 1 cut(s) 783
Psp6I CCWGG 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 110, 390
PsuI RGATCY 2 cut(s) 37, 85
PsyI GACNNNGTC 1 cut(s) 752
RsaI GTAC 2 cut(s) 743, 813
RsaNI GTAC 2 cut(s) 742, 812
SacI GAGCTC 1 cut(s) 783
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 3 cut(s) 37, 85, 400
ScrFI CCNGG 2 cut(s) 80, 351
SduI GDGCHC 2 cut(s) 543, 783
Sfr274I CTCGAG 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
SphI GCATGC 1 cut(s) 306
Sse9I AATT 3 cut(s) 198, 490, 762
SseBI AGGCCT 1 cut(s) 359
SspI AATATT 2 cut(s) 31, 469
SspMI CTAG 3 cut(s) 6, 89, 725
SstI GAGCTC 1 cut(s) 783
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 78, 349
StyI CCWWGG 2 cut(s) 271, 585
TaaI ACNGT 2 cut(s) 118, 488
TaqI TCGA 5 cut(s) 183, 237, 316, 546, 768
TasI AATT 3 cut(s) 198, 490, 762
TfiI GAWTC 2 cut(s) 311, 653
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 1 cut(s) 666
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 666
TspDTI ATGAA 4 cut(s) 352, 541, 573, 642
TspRI CASTG 1 cut(s) 675
Tth111I GACNNNGTC 1 cut(s) 752
XapI RAATTY 1 cut(s) 762
XceI RCATGY 3 cut(s) 306, 335, 416
XhoI CTCGAG 1 cut(s) 315
XspI CTAG 3 cut(s) 6, 89, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.