Rw5G039380

carbonate dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000548
Physical Location & Seq
Reverse (-)
69452744 .. 69455669
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039380.1

Sequence Viewer

Length: 699 bp
ATGAAAAAAGAATTAAAGAATGAAAACCAGGATGATGTGGTTGCTACTGAAACTGAGAGCCTGGAAGATGACCCAGTTCAAACAATTTTAGACGGGTTCAAGCACTTCAAGACCAACTTTTACGACACAAACCCAGAGTTGTTCAACGAGCTTGCCCTAGCACAAAGCCCCAAGTTTTTGGTATTTGCATGCTCGGATTCCCGAGTGAGTCCCTCGCATGTTCTTCATTGCACACCTGGACAAGCCTTTCTGGTTCGCAACATTGCTAACTTGGTTCCTGCATATGACCAGCTAAGGTACACAGGAACTGGAGCAACCATTGAGTATGCTGTAGAAGAACTCGGGGTAGAAAACATTTTGATAGTGGGACACAGTAGTTGCGGAGGAATAAAAAGGCTAATGTCTTATCCCGAGGATGGTTCCGCTCCACTGGTCTTTATAGATGATTGGATGAAAATGGCTTTACCTGTCAAGACAAAGGTTATTGCGGAAGCTGGCGATACCGATTTCCAGGAACAATGTGATCGTTGCGCAAGGAAAGCAGTAAATTTGTCATTGACAAACCTACTCACATATCCCTTCGTTCAAGAGGCACACTCGGAGAAAAAACTAGCACTTCGGGGTGCTTACTATGACTTTGTCAACGGACGTTTCGAGCTCTGGGAGCATGGTTCCGACAACATCATCATAGCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

232

Amino Acids

25.94

Weight (kDa)

4.85

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 60 - 217 3.1e-42 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 532
AccBSI CCGCTC 1 cut(s) 425
AciI CCGC 3 cut(s) 381, 423, 488
AcsI RAATTY 1 cut(s) 547
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 416
AgsI TTSAA 5 cut(s) 80, 100, 109, 145, 587
AjnI CCWGG 4 cut(s) 27, 60, 235, 510
AluBI AGCT 4 cut(s) 151, 292, 494, 658
AluI AGCT 4 cut(s) 151, 292, 494, 658
Alw21I GWGCWC 1 cut(s) 660
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 3 cut(s) 201, 341, 410
ApoI RAATTY 1 cut(s) 547
AspLEI GCGC 1 cut(s) 533
AvaI CYCGRG 3 cut(s) 201, 341, 410
BanII GRGCYC 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 660
BccI CCATC 1 cut(s) 410
BciT130I CCWGG 4 cut(s) 29, 62, 237, 512
BfaI CTAG 2 cut(s) 158, 611
BfmI CTRYAG 1 cut(s) 330
Bme1390I CCNGG 4 cut(s) 29, 62, 237, 512
BmeT110I CYCGRG 3 cut(s) 201, 341, 410
BmiI GGNNCC 3 cut(s) 276, 421, 673
BmrFI CCNGG 4 cut(s) 29, 62, 237, 512
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 411
Bsc4I CCNNNNNNNGG 1 cut(s) 416
Bse1I ACTGG 3 cut(s) 74, 313, 435
Bse3DI GCAATG 2 cut(s) 226, 261
BseBI CCWGG 4 cut(s) 29, 62, 237, 512
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 3 cut(s) 37, 421, 456
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMI GCAATG 2 cut(s) 226, 261
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 3 cut(s) 74, 313, 435
BsiHKAI GWGCWC 1 cut(s) 660
BsiHKCI CYCGRG 3 cut(s) 201, 341, 410
BslFI GGGAC 2 cut(s) 195, 381
BslI CCNNNNNNNGG 1 cut(s) 416
BsmFI GGGAC 2 cut(s) 195, 381
BsoBI CYCGRG 3 cut(s) 201, 341, 410
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 1 cut(s) 523
BspACI CCGC 3 cut(s) 381, 423, 488
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 276, 421, 673
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 2 cut(s) 226, 261
BsrI ACTGG 3 cut(s) 74, 313, 435
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 1 cut(s) 523
Bst2UI CCWGG 4 cut(s) 29, 62, 237, 512
Bst4CI ACNGT 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 153, 190, 496
BstDEI CTNAG 3 cut(s) 54, 293, 696
BstF5I GGATG 3 cut(s) 37, 421, 456
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 1 cut(s) 526
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 2 cut(s) 539, 664
BstNI CCWGG 4 cut(s) 29, 62, 237, 512
BstNSI RCATGY 2 cut(s) 192, 221
BstSCI CCNGG 4 cut(s) 27, 60, 235, 510
BstSFI CTRYAG 1 cut(s) 330
BstXI CCANNNNNNTGG 1 cut(s) 178
BtsCI GGATG 3 cut(s) 37, 421, 456
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 3 cut(s) 153, 190, 496
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 298
CviAII CATG 3 cut(s) 189, 218, 668
CviJI RGCY 9 cut(s) 60, 151, 168, 245, 292, 397, 461, 494, 658
CviKI_1 RGCY 9 cut(s) 60, 151, 168, 245, 292, 397, 461, 494, 658
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 3 cut(s) 54, 293, 696
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
Ecl136II GAGCTC 1 cut(s) 658
Eco24I GRGCYC 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 658
Eco88I CYCGRG 3 cut(s) 201, 341, 410
EcoICRI GAGCTC 1 cut(s) 658
EcoRII CCWGG 4 cut(s) 27, 60, 235, 510
EcoT38I GRGCYC 1 cut(s) 660
FaeI CATG 3 cut(s) 192, 221, 671
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 2 cut(s) 195, 381
FatI CATG 3 cut(s) 188, 217, 667
FauNDI CATATG 1 cut(s) 283
FokI GGATG 3 cut(s) 44, 428, 463
FriOI GRGCYC 1 cut(s) 660
FspBI CTAG 2 cut(s) 158, 611
FspI TGCGCA 1 cut(s) 532
GlaI GCGC 1 cut(s) 532
GsuI CTGGAG 1 cut(s) 330
HhaI GCGC 1 cut(s) 533
Hin1II CATG 3 cut(s) 192, 221, 671
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HinfI GANTC 2 cut(s) 197, 208
Hpy166II GTNNAC 2 cut(s) 300, 643
Hpy188I TCNGA 3 cut(s) 196, 601, 676
Hpy188III TCNNGA 5 cut(s) 109, 201, 410, 472, 587
Hpy8I GTNNAC 2 cut(s) 300, 643
HpyAV CCTTC 1 cut(s) 589
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 3 cut(s) 188, 231, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 664
HpyF3I CTNAG 3 cut(s) 54, 293, 696
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 3 cut(s) 192, 221, 671
HspAI GCGC 1 cut(s) 531
Kzo9I GATC 1 cut(s) 523
LmnI GCTCC 3 cut(s) 311, 430, 664
MaeI CTAG 2 cut(s) 158, 611
MaeII ACGT 1 cut(s) 649
MalI GATC 1 cut(s) 525
MbiI CCGCTC 1 cut(s) 425
MboI GATC 1 cut(s) 523
MboII GAAGA 3 cut(s) 77, 215, 347
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 3 cut(s) 11, 84, 547
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 4 cut(s) 223, 377, 406, 583
MseI TTAA 1 cut(s) 14
MspR9I CCNGG 4 cut(s) 29, 62, 237, 512
MvaI CCWGG 4 cut(s) 29, 62, 237, 512
MwoI GCNNNNNNNGC 2 cut(s) 539, 664
NdeI CATATG 1 cut(s) 283
NdeII GATC 1 cut(s) 523
NlaIII CATG 3 cut(s) 192, 221, 671
NlaIV GGNNCC 3 cut(s) 276, 421, 673
NsbI TGCGCA 1 cut(s) 532
NspI RCATGY 2 cut(s) 192, 221
PaeI GCATGC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PfeI GAWTC 1 cut(s) 197
PflFI GACNNNGTC 1 cut(s) 638
PfoI TCCNGGA 1 cut(s) 510
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 660
Psp6I CCWGG 4 cut(s) 27, 60, 235, 510
PspGI CCWGG 4 cut(s) 27, 60, 235, 510
PspN4I GGNNCC 3 cut(s) 276, 421, 673
PstNI CAGNNNCTG 1 cut(s) 308
PsyI GACNNNGTC 1 cut(s) 638
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SacI GAGCTC 1 cut(s) 660
SaqAI TTAA 1 cut(s) 14
Sau3AI GATC 1 cut(s) 523
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 4 cut(s) 29, 62, 237, 512
SduI GDGCHC 1 cut(s) 660
SfcI CTRYAG 1 cut(s) 330
SphI GCATGC 1 cut(s) 192
Sse9I AATT 3 cut(s) 11, 84, 547
SsiI CCGC 3 cut(s) 381, 423, 488
SspMI CTAG 2 cut(s) 158, 611
SstI GAGCTC 1 cut(s) 660
StyD4I CCNGG 4 cut(s) 27, 60, 235, 510
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 652
TaqI TCGA 1 cut(s) 654
TasI AATT 3 cut(s) 11, 84, 547
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 1 cut(s) 435
TspDTI ATGAA 4 cut(s) 17, 36, 215, 467
TspGWI ACGGA 1 cut(s) 660
TspRI CASTG 1 cut(s) 435
Tth111I GACNNNGTC 1 cut(s) 638
XapI RAATTY 1 cut(s) 547
XceI RCATGY 2 cut(s) 192, 221
XspI CTAG 2 cut(s) 158, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.