Rh5AG418900

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000548
Physical Location & Seq
Reverse (-)
72272011 .. 72273415
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG418900.1

Sequence Viewer

Length: 702 bp
ATGGTTGAGTGCAATGACCTGAAAGGAGCTGAACCAAATGCACCGACCAGTCCAATAGCCAGCCCTCTCGACCCAGTTAAAAGAATTATAGATGGCTTCAAGCACTTCAAGACCAGCTACTTCGACACAAATCCAGCTTTGTTTGATGAGCTTGCCCAAGGACAAAGCCCCGAGTTTATGGTATTTGCATGCTCGGATTCTCGAGTGTGTCCTTCACATGTTCTTCACTTCCAACCTGGGGAGGCCTTCATCGTTCGAAACATTGCTAACATGGTTCCTGCTTTTGATCAGCTAAAACATGCAGGAGTTGGAGCAACCATAGAATATGCTGTCAAAGAACTCGGGGTGGCAAATATTTTGGTAATGGGACACAGTAATTGTGGTGGAATAAAGAGGCTAATGTCTTATCCGGAGGATGGCTCTGAGCCCTTCGACTTTATAGATGAATGGGTGAAAATGAGTTTACCAGCCAAGGCTGAGGTTATTGCAGAAGCTGGGAGTGCAGATTTCCATGAACAATGTGAAAGATGTGCAAGGGAATCAGTAAACTTGTCACTGGCAAACCGACTTACCTACCCCTTTGTTCAAAAGGCACACTTGGATGAAAAACTAGCACTTCGGGGTGGATACTATGACTTTGTCAATGGAATTTTCGAGCTATGGGAGCTCAAATCCCACATTTCAAACCTCGTACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

233

Amino Acids

25.8

Weight (kDa)

5.14

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 60 - 216 2.6e-40 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 409
AcsI RAATTY 1 cut(s) 648
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 2 cut(s) 238, 416
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 4 cut(s) 100, 109, 587, 684
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 8 cut(s) 29, 117, 137, 151, 292, 494, 658, 667
AluI AGCT 8 cut(s) 29, 117, 137, 151, 292, 494, 658, 667
Alw21I GWGCWC 1 cut(s) 669
AlwNI CAGNNNCTG 1 cut(s) 494
Ama87I CYCGRG 3 cut(s) 170, 201, 341
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 648
AsuHPI GGTGA 1 cut(s) 463
AsuII TTCGAA 1 cut(s) 256
AvaI CYCGRG 3 cut(s) 170, 201, 341
BanII GRGCYC 2 cut(s) 429, 669
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 477
BccI CCATC 2 cut(s) 86, 410
BciT130I CCWGG 1 cut(s) 237
BciVI GTATCC 1 cut(s) 620
BclI TGATCA 1 cut(s) 286
BfaI CTAG 1 cut(s) 611
BfuI GTATCC 1 cut(s) 620
Bme1390I CCNGG 1 cut(s) 237
BmeT110I CYCGRG 3 cut(s) 170, 201, 341
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 237
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 404, 436
Bpu10I CCTNAGC 1 cut(s) 477
Bpu14I TTCGAA 1 cut(s) 256
BsaJI CCNNGG 3 cut(s) 157, 236, 471
BsaWI WCCGGW 1 cut(s) 409
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 416
Bse1I ACTGG 3 cut(s) 48, 74, 561
Bse3DI GCAATG 2 cut(s) 19, 261
BseAI TCCGGA 1 cut(s) 409
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 157, 236, 471
BseGI GGATG 2 cut(s) 421, 607
BseLI CCNNNNNNNGG 2 cut(s) 238, 416
BseMI GCAATG 2 cut(s) 19, 261
BseMII CTCAG 2 cut(s) 414, 468
BseNI ACTGG 3 cut(s) 48, 74, 561
BseYI CCCAGC 1 cut(s) 494
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 1 cut(s) 245
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 3 cut(s) 170, 201, 341
BsiSI CCGG 1 cut(s) 410
BslFI GGGAC 1 cut(s) 381
BslI CCNNNNNNNGG 2 cut(s) 238, 416
BsmFI GGGAC 1 cut(s) 381
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 3 cut(s) 170, 201, 341
Bsp119I TTCGAA 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 429, 669
Bsp13I TCCGGA 1 cut(s) 409
Bsp143I GATC 1 cut(s) 286
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 2 cut(s) 415, 469
BspEI TCCGGA 1 cut(s) 409
BspLI GGNNCC 1 cut(s) 276
BspT104I TTCGAA 1 cut(s) 256
BsrDI GCAATG 2 cut(s) 19, 261
BsrI ACTGG 3 cut(s) 48, 74, 561
BssECI CCNNGG 3 cut(s) 157, 236, 471
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 2 cut(s) 157, 471
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 3 cut(s) 61, 153, 190
BstDEI CTNAG 2 cut(s) 423, 477
BstF5I GGATG 2 cut(s) 421, 607
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 2 cut(s) 500, 664
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 3 cut(s) 192, 221, 302
BstSCI CCNGG 1 cut(s) 235
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 245
BtsCI GGATG 2 cut(s) 421, 607
BtsIMutI CAGTG 1 cut(s) 554
Cac8I GCNNGC 3 cut(s) 61, 153, 190
CaiI CAGNNNCTG 1 cut(s) 494
Csp6I GTAC 1 cut(s) 692
CviAII CATG 5 cut(s) 189, 218, 271, 299, 512
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 2 cut(s) 423, 477
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 2 cut(s) 157, 471
Eco147I AGGCCT 1 cut(s) 245
Eco24I GRGCYC 2 cut(s) 429, 669
Eco53kI GAGCTC 1 cut(s) 667
Eco88I CYCGRG 3 cut(s) 170, 201, 341
EcoICRI GAGCTC 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 157, 471
EcoT38I GRGCYC 2 cut(s) 429, 669
ErhI CCWWGG 2 cut(s) 157, 471
FaeI CATG 5 cut(s) 192, 221, 274, 302, 515
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FaqI GGGAC 1 cut(s) 381
FatI CATG 5 cut(s) 188, 217, 270, 298, 511
FbaI TGATCA 1 cut(s) 286
FokI GGATG 2 cut(s) 428, 614
FriOI GRGCYC 2 cut(s) 429, 669
FspBI CTAG 1 cut(s) 611
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 1 cut(s) 245
HapII CCGG 1 cut(s) 410
Hin1II CATG 5 cut(s) 192, 221, 274, 302, 515
HinfI GANTC 2 cut(s) 197, 539
HpaII CCGG 1 cut(s) 410
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 2 cut(s) 464, 547
Hpy188I TCNGA 2 cut(s) 196, 424
Hpy188III TCNNGA 5 cut(s) 68, 109, 201, 410, 699
Hpy8I GTNNAC 2 cut(s) 464, 547
HpyAV CCTTC 3 cut(s) 222, 256, 439
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4V TGCA 7 cut(s) 12, 41, 188, 302, 488, 503, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 664
HpyF3I CTNAG 2 cut(s) 423, 477
Hsp92II CATG 5 cut(s) 192, 221, 274, 302, 515
Kpn2I TCCGGA 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 3 cut(s) 26, 311, 664
MaeI CTAG 1 cut(s) 611
MaeIII GTNAC 1 cut(s) 552
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 1 cut(s) 215
MhlI GDGCHC 2 cut(s) 429, 669
MluCI AATT 3 cut(s) 84, 376, 648
MmeI TCCRAC 2 cut(s) 256, 289
MnlI CCTC 6 cut(s) 75, 235, 387, 406, 472, 698
MroI TCCGGA 1 cut(s) 409
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 600
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 500, 664
NdeII GATC 1 cut(s) 286
NlaIII CATG 5 cut(s) 192, 221, 274, 302, 515
NlaIV GGNNCC 1 cut(s) 276
NmuCI GTSAC 1 cut(s) 552
NspI RCATGY 3 cut(s) 192, 221, 302
NspV TTCGAA 1 cut(s) 256
PaeI GCATGC 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 201
PceI AGGCCT 1 cut(s) 245
PciI ACATGT 1 cut(s) 217
PfeI GAWTC 2 cut(s) 197, 539
PflFI GACNNNGTC 1 cut(s) 638
PscI ACATGT 1 cut(s) 217
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 494
PsyI GACNNNGTC 1 cut(s) 638
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 1 cut(s) 78
Sau3AI GATC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 2 cut(s) 429, 669
Sfr274I CTCGAG 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 256
SlaI CTCGAG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
SphI GCATGC 1 cut(s) 192
Sse9I AATT 3 cut(s) 84, 376, 648
SseBI AGGCCT 1 cut(s) 245
SspI AATATT 1 cut(s) 355
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 1 cut(s) 669
StuI AGGCCT 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 235
StyI CCWWGG 2 cut(s) 157, 471
TaaI ACNGT 1 cut(s) 374
TaqI TCGA 6 cut(s) 69, 123, 202, 256, 432, 654
TasI AATT 3 cut(s) 84, 376, 648
TfiI GAWTC 2 cut(s) 197, 539
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 1 cut(s) 552
Tsp45I GTSAC 1 cut(s) 552
TspDTI ATGAA 4 cut(s) 238, 459, 528, 618
TspRI CASTG 1 cut(s) 561
Tth111I GACNNNGTC 1 cut(s) 638
XapI RAATTY 1 cut(s) 648
XceI RCATGY 3 cut(s) 192, 221, 302
XhoI CTCGAG 1 cut(s) 201
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.