Rroxscaffold_7G00192380

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000548
Physical Location & Seq
Reverse (-)
33555637 .. 33558733
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192380.1

Sequence Viewer

Length: 939 bp
ATGGCTAGCCAGTTGGCAATTCATCGATTGAACAAGTTCCTCAGTGAGAAGGAGGACTTGGATGAAGTGGCTGCTGCCAAAATTGACAAACTAATTGCTGAGCTGCAAACGCCCGGTGATCATTTTGATCCGTGCGGACGTCCATCCTCATGCCTCGATCAGGCGTCAACAGCGGTGCTGCTCTTGCCAGACGGAGATCACGGTATCTCGGACGGCATCACCATCAAGATGAAGGCCAAGCCGACGAGTCCACAGGCAAAAAGAGGCTTAAGATCAAGGCCTGGTCTCCGTCTGGCAAGAGCAGCGCCACCGTCGATACCTGATCGAGGCCTGAGGATGGACGTCCGCACGTTTGCATGTTTGCAGACGTCGTCTTCTGATCCTCCCAAATATCCATGTCTGTTCGACAAGCTTGCCTTAGGACAAAGCCCCAAATTTCTGGTGTTCGCATGCTCAGACTCTCGAGTGAGCCCCTCTATTATCCTTAACTTTAAACCTGGGGAGGCCTTCATGGTTCGCAACATTGCTAACTTGGTTCCTACATTTAGCCAGCAAAGATACTCGGGAACTGGAGCAATTATAGAATATGCCGTCACACAACTCAAGGTAGAAAACATTTTGGTTATTGGGCATAGTAAATGTGGTGGGATACAGAGGCTTATGGAACATCCAGAAGATGGCAGTGTTCCCTTCGACTTCATAGATGACTGGGTTAAAATTGCCCAGGATGCAAAGGATAAAGTTAAAGCAGAGGGAGGAGGACATGAGGCTTGTGCTAGGGAAGCTGTAAATGTCTCTCTGGAAAACCTGCTAACTTATCCTTACGTTCATAAGGAGGTATCGGACAGAAAAATAGCACTTAGAGGTGGTTACTATGACTTCGTCAATGGAGTTTTCGAGCTCTGGGAGAAACATGAATCTCACATTTCACCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

312

Amino Acids

34.27

Weight (kDa)

6.76

Isoelectric Point (pI)

54.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 147 - 297 1.4e-39 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 142, 345, 371
Acc36I ACCTGC 1 cut(s) 816
AccB7I CCANNNNNTGG 1 cut(s) 677
AciI CCGC 3 cut(s) 135, 173, 346
AclWI GGATC 2 cut(s) 122, 374
AcsI RAATTY 1 cut(s) 434
AcyI GRCGYC 4 cut(s) 139, 164, 342, 368
AfiI CCNNNNNNNGG 4 cut(s) 160, 326, 337, 677
AflII CTTAAG 1 cut(s) 268
AgsI TTSAA 1 cut(s) 31
AjnI CCWGG 3 cut(s) 280, 496, 723
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 4 cut(s) 103, 412, 785, 901
AluI AGCT 4 cut(s) 103, 412, 785, 901
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 2 cut(s) 290, 799
AlwI GGATC 2 cut(s) 122, 374
Ama87I CYCGRG 2 cut(s) 462, 562
AoxI GGCC 4 cut(s) 234, 278, 328, 504
ApeKI GCWGC 5 cut(s) 71, 74, 103, 178, 302
ApoI RAATTY 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 35
AspLEI GCGC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 128, 211, 921
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 2 cut(s) 462, 562
AxyI CCTNAGG 2 cut(s) 332, 418
BanII GRGCYC 2 cut(s) 473, 903
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 5 cut(s) 58, 61, 90, 165, 314
BccI CCATC 4 cut(s) 151, 230, 331, 671
BceAI ACGGC 2 cut(s) 229, 575
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 3 cut(s) 282, 498, 725
BciVI GTATCC 1 cut(s) 642
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 290, 799
BfaI CTAG 2 cut(s) 6, 777
BfoI RGCGCY 1 cut(s) 308
BfrI CTTAAG 1 cut(s) 268
BfuAI ACCTGC 1 cut(s) 816
BfuI GTATCC 1 cut(s) 642
BisI GCNGC 5 cut(s) 72, 75, 104, 179, 303
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 5 cut(s) 73, 76, 105, 180, 304
Bme1390I CCNGG 4 cut(s) 114, 282, 498, 725
BmeT110I CYCGRG 2 cut(s) 462, 562
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 4 cut(s) 114, 282, 498, 725
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 2 cut(s) 225, 718
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 591
Bpu1102I GCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 587
BpuMI CCSGG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 25
BsaHI GRCGYC 4 cut(s) 139, 164, 342, 368
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 497, 723
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 326, 337, 677
Bse1I ACTGG 3 cut(s) 10, 574, 713
Bse21I CCTNAGG 2 cut(s) 332, 418
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 3 cut(s) 282, 498, 725
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 2 cut(s) 497, 723
BseGI GGATG 5 cut(s) 67, 143, 342, 667, 733
BseLI CCNNNNNNNGG 4 cut(s) 160, 326, 337, 677
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 4 cut(s) 55, 90, 323, 468
BseNI ACTGG 3 cut(s) 10, 574, 713
BseRI GAGGAG 1 cut(s) 771
BseXI GCAGC 5 cut(s) 58, 61, 90, 165, 314
BshFI GGCC 4 cut(s) 236, 280, 330, 506
BshVI ATCGAT 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 2 cut(s) 462, 562
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 4 cut(s) 160, 326, 337, 677
BsmAI GTCTC 2 cut(s) 290, 799
BsnI GGCC 4 cut(s) 236, 280, 330, 506
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 2 cut(s) 462, 562
Bsp1286I GDGCHC 2 cut(s) 473, 903
Bsp143I GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 3 cut(s) 135, 173, 346
BspANI GGCC 4 cut(s) 236, 280, 330, 506
BspCNI CTCAG 4 cut(s) 54, 91, 324, 467
BspDI ATCGAT 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 537
BspMI ACCTGC 1 cut(s) 816
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 122, 374
BspTI CTTAAG 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 522
BsrI ACTGG 3 cut(s) 10, 574, 713
BssECI CCNNGG 2 cut(s) 497, 723
BssMI GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
BssNI GRCGYC 4 cut(s) 139, 164, 342, 368
Bst2UI CCWGG 3 cut(s) 282, 498, 725
Bst4CI ACNGT 2 cut(s) 203, 312
BstACI GRCGYC 4 cut(s) 139, 164, 342, 368
BstAFI CTTAAG 1 cut(s) 268
BstC8I GCNNGC 4 cut(s) 7, 414, 451, 551
BstDEI CTNAG 6 cut(s) 41, 99, 332, 418, 454, 860
BstENI CCTNNNNNAGG 2 cut(s) 158, 324
BstF5I GGATG 5 cut(s) 67, 143, 342, 667, 733
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 7 cut(s) 121, 130, 160, 199, 275, 325, 382
BstMAI GTCTC 2 cut(s) 290, 799
BstMBI GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
BstMWI GCNNNNNNNGC 6 cut(s) 109, 170, 184, 302, 728, 782
BstNI CCWGG 3 cut(s) 282, 498, 725
BstNSI RCATGY 2 cut(s) 360, 453
BstSCI CCNGG 4 cut(s) 112, 280, 496, 723
BstV1I GCAGC 5 cut(s) 58, 61, 90, 165, 314
BstV2I GAAGAC 1 cut(s) 366
BstXI CCANNNNNNTGG 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 25
Bsu36I CCTNAGG 2 cut(s) 332, 418
BsuI GTATCC 1 cut(s) 642
BsuRI GGCC 4 cut(s) 236, 280, 330, 506
BsuTUI ATCGAT 1 cut(s) 25
BtsCI GGATG 5 cut(s) 67, 143, 342, 667, 733
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 2 cut(s) 49, 688
BveI ACCTGC 1 cut(s) 816
Cac8I GCNNGC 4 cut(s) 7, 414, 451, 551
CfoI GCGC 1 cut(s) 307
ClaI ATCGAT 1 cut(s) 25
CseI GACGC 1 cut(s) 153
CviAII CATG 8 cut(s) 150, 357, 396, 450, 511, 764, 914, 936
DdeI CTNAG 6 cut(s) 41, 99, 332, 418, 454, 860
DpnI GATC 7 cut(s) 120, 129, 159, 198, 274, 324, 381
DpnII GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
DraI TTTAAA 1 cut(s) 493
Ecl136II GAGCTC 1 cut(s) 901
Eco147I AGGCCT 3 cut(s) 280, 330, 506
Eco24I GRGCYC 2 cut(s) 473, 903
Eco31I GGTCTC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 901
Eco81I CCTNAGG 2 cut(s) 332, 418
Eco88I CYCGRG 2 cut(s) 462, 562
EcoICRI GAGCTC 1 cut(s) 901
EcoNI CCTNNNNNAGG 2 cut(s) 158, 324
EcoRII CCWGG 3 cut(s) 280, 496, 723
EcoT38I GRGCYC 2 cut(s) 473, 903
FaeI CATG 8 cut(s) 153, 360, 399, 453, 514, 767, 917, 939
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 401, 433
FatI CATG 8 cut(s) 149, 356, 395, 449, 510, 763, 913, 935
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 5 cut(s) 72, 75, 104, 179, 303
FokI GGATG 5 cut(s) 74, 130, 349, 654, 740
FriOI GRGCYC 2 cut(s) 473, 903
Fsp4HI GCNGC 5 cut(s) 72, 75, 104, 179, 303
FspBI CTAG 2 cut(s) 6, 777
GlaI GCGC 1 cut(s) 306
GluI GCNGC 5 cut(s) 72, 75, 104, 179, 303
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 4 cut(s) 236, 280, 330, 506
HapII CCGG 1 cut(s) 114
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 4 cut(s) 139, 164, 342, 368
Hin1II CATG 8 cut(s) 153, 360, 399, 453, 514, 767, 917, 939
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 3 cut(s) 247, 458, 917
HpaII CCGG 1 cut(s) 114
HphI GGTGA 3 cut(s) 128, 211, 921
Hpy166II GTNNAC 2 cut(s) 168, 251
Hpy188I TCNGA 4 cut(s) 211, 379, 457, 844
Hpy188III TCNNGA 5 cut(s) 226, 462, 564, 671, 800
Hpy8I GTNNAC 2 cut(s) 168, 251
Hpy99I CGWCG 3 cut(s) 247, 316, 373
HpyAV CCTTC 4 cut(s) 43, 226, 517, 700
HpyCH4III ACNGT 2 cut(s) 203, 312
HpyCH4IV ACGT 5 cut(s) 139, 342, 350, 368, 825
HpyCH4V TGCA 4 cut(s) 106, 356, 364, 731
HpyF10VI GCNNNNNNNGC 6 cut(s) 109, 170, 184, 302, 728, 782
HpyF3I CTNAG 6 cut(s) 41, 99, 332, 418, 454, 860
HpySE526I ACGT 5 cut(s) 139, 342, 350, 368, 825
Hsp92I GRCGYC 4 cut(s) 139, 164, 342, 368
Hsp92II CATG 8 cut(s) 153, 360, 399, 453, 514, 767, 917, 939
HspAI GCGC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
LmnI GCTCC 1 cut(s) 572
Lsp1109I GCAGC 5 cut(s) 58, 61, 90, 165, 314
LweI GCATC 2 cut(s) 225, 718
MaeI CTAG 2 cut(s) 6, 777
MaeII ACGT 5 cut(s) 139, 342, 350, 368, 825
MaeIII GTNAC 2 cut(s) 592, 869
MalI GATC 7 cut(s) 120, 129, 159, 198, 274, 324, 381
MboI GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
MboII GAAGA 2 cut(s) 366, 686
MhlI GDGCHC 2 cut(s) 473, 903
MluCI AATT 6 cut(s) 18, 81, 93, 434, 576, 717
MlyI GAGTC 2 cut(s) 256, 452
MroXI GAANNNNTTC 1 cut(s) 35
MseI TTAA 5 cut(s) 269, 486, 492, 714, 744
MslI CAYNNNNRTG 3 cut(s) 148, 227, 934
MspA1I CMGCKG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 268
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 4 cut(s) 114, 282, 498, 725
MvaI CCWGG 3 cut(s) 282, 498, 725
MwoI GCNNNNNNNGC 6 cut(s) 109, 170, 184, 302, 728, 782
NciI CCSGG 1 cut(s) 114
NdeII GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 8 cut(s) 153, 360, 399, 453, 514, 767, 917, 939
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 2 cut(s) 360, 453
PaeI GCATGC 1 cut(s) 453
PaeR7I CTCGAG 1 cut(s) 462
PceI AGGCCT 3 cut(s) 280, 330, 506
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 917
PflFI GACNNNGTC 2 cut(s) 370, 881
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 5 cut(s) 73, 76, 105, 180, 304
PleI GAGTC 2 cut(s) 255, 452
PpsI GAGTC 2 cut(s) 255, 452
Psp124BI GAGCTC 1 cut(s) 903
Psp6I CCWGG 3 cut(s) 280, 496, 723
PspGI CCWGG 3 cut(s) 280, 496, 723
PspN4I GGNNCC 1 cut(s) 537
PsyI GACNNNGTC 2 cut(s) 370, 881
RseI CAYNNNNRTG 3 cut(s) 148, 227, 934
SacI GAGCTC 1 cut(s) 903
SaqAI TTAA 5 cut(s) 269, 486, 492, 714, 744
SatI GCNGC 5 cut(s) 72, 75, 104, 179, 303
Sau3AI GATC 7 cut(s) 118, 127, 157, 196, 272, 322, 379
SchI GAGTC 2 cut(s) 256, 452
ScrFI CCNGG 4 cut(s) 114, 282, 498, 725
SduI GDGCHC 2 cut(s) 473, 903
SfaNI GCATC 2 cut(s) 225, 718
Sfr274I CTCGAG 1 cut(s) 462
SlaI CTCGAG 1 cut(s) 462
SmiMI CAYNNNNRTG 3 cut(s) 148, 227, 934
SmlI CTYRAG 3 cut(s) 268, 462, 602
SmoI CTYRAG 3 cut(s) 268, 462, 602
SphI GCATGC 1 cut(s) 453
Sse9I AATT 6 cut(s) 18, 81, 93, 434, 576, 717
SseBI AGGCCT 3 cut(s) 280, 330, 506
SsiI CCGC 3 cut(s) 135, 173, 346
SspMI CTAG 2 cut(s) 6, 777
SstI GAGCTC 1 cut(s) 903
StuI AGGCCT 3 cut(s) 280, 330, 506
StyD4I CCNGG 4 cut(s) 112, 280, 496, 723
TaaI ACNGT 2 cut(s) 203, 312
TaiI ACGT 5 cut(s) 142, 345, 353, 371, 828
TaqI TCGA 8 cut(s) 25, 156, 314, 325, 405, 463, 693, 897
TasI AATT 6 cut(s) 18, 81, 93, 434, 576, 717
TfiI GAWTC 1 cut(s) 917
Tru1I TTAA 5 cut(s) 269, 486, 492, 714, 744
Tru9I TTAA 5 cut(s) 269, 486, 492, 714, 744
TscAI CASTG 2 cut(s) 49, 688
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 5 cut(s) 71, 74, 103, 178, 302
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 7 cut(s) 11, 78, 245, 499, 688, 818, 930
TspGWI ACGGA 3 cut(s) 120, 207, 278
TspRI CASTG 2 cut(s) 49, 688
Tth111I GACNNNGTC 2 cut(s) 370, 881
Van91I CCANNNNNTGG 1 cut(s) 677
Vha464I CTTAAG 1 cut(s) 268
XagI CCTNNNNNAGG 2 cut(s) 158, 324
XapI RAATTY 1 cut(s) 434
XceI RCATGY 2 cut(s) 360, 453
XhoI CTCGAG 1 cut(s) 462
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 2 cut(s) 6, 777
ZraI GACGTC 3 cut(s) 140, 343, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.