Rh6DG208000

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000548
Physical Location & Seq
Forward (+)
36951349 .. 36956126
4778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG208000.1

Sequence Viewer

Length: 765 bp
ATGGCTAGCCAGTTGGCAATTCATCGTTTGAACAAGTTCCTCAGTGAGAAGGAGGACTTGGATGAAGTGGCTGCTGCCAAAATTGACAAACTAATTGCTGAGCTGCAAACGCCCGGTGATCATTTTGATCCGGTTCAAACGATTGTAAATGGGTTCATGGACTTCAAGATCCACAAATTCGACAAATATCCATGTCTGTTCGACAAGCTTGCCTTAGGACAAAGCCCCAAATTTCTGGTGTTCGCATGCTCAGACTCTCGAGTGAGCCCCTCTATTATCCTTAACTTTAAACCTGGGGAGGCCTTCATGGTTCGCAACATTGCTAACTTGGTTCCTACATTTAGCCAGCTAAGATACTCGGGAACTGGAGCAATTATAGAATATGCCGTCACACAACTCAAGGTAGAAAACATTTTGGTTATTGGGCATAGTAAATGTGGTGGGATACAGAGGCTTATGGAACATCCAGAAGATGGCAGTGTTCCCTTCGACTTCATAGATGACTGGGTTAAAATTGCCCAGGATGCCAAGGATAAAGTTAAAGCAGAGGGAGGAGGACATGAGGCTTGTGCTAGGGAAGCTGTAAATGTCTCTCTGGAAAACCTGCTAACTTATCCTTACGTTCATAAGGAGGTATCGGAAAGAAAAATAGCACTTAGAGGTGGTTACTATGACTTCGTCAATGGAGTTTTCGAGCTCTGGGAGAAACACGAATCTCACATTTCACCCCCCATGATCATTCCGCCTCCACAATTAAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

254

Amino Acids

28.49

Weight (kDa)

5.86

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 79 - 229 5.3e-40 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 612
AccB7I CCANNNNNTGG 1 cut(s) 473
AciI CCGC 1 cut(s) 743
AclWI GGATC 2 cut(s) 122, 163
AcsI RAATTY 2 cut(s) 176, 230
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 3 cut(s) 31, 137, 166
AjnI CCWGG 2 cut(s) 292, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 5 cut(s) 103, 208, 349, 581, 697
AluI AGCT 5 cut(s) 103, 208, 349, 581, 697
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 2 cut(s) 122, 163
Ama87I CYCGRG 2 cut(s) 258, 358
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 71, 74, 103
ApoI RAATTY 2 cut(s) 176, 230
Asp700I GAANNNNTTC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 128, 717
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 2 cut(s) 258, 358
AxyI CCTNAGG 1 cut(s) 214
BanII GRGCYC 2 cut(s) 269, 699
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 3 cut(s) 58, 61, 90
BccI CCATC 1 cut(s) 467
BceAI ACGGC 1 cut(s) 371
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 2 cut(s) 294, 521
BciVI GTATCC 1 cut(s) 438
BclI TGATCA 2 cut(s) 118, 735
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 1 cut(s) 595
BfaI CTAG 2 cut(s) 6, 573
BfuAI ACCTGC 1 cut(s) 612
BfuI GTATCC 1 cut(s) 438
BisI GCNGC 3 cut(s) 72, 75, 104
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 3 cut(s) 73, 76, 105
Bme1390I CCNGG 3 cut(s) 114, 294, 521
BmeT110I CYCGRG 2 cut(s) 258, 358
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 3 cut(s) 114, 294, 521
BmrI ACTGGG 1 cut(s) 514
BmsI GCATC 1 cut(s) 514
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 514
BpmI CTGGAG 1 cut(s) 387
Bpu1102I GCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 293, 519, 528
BsaWI WCCGGW 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse1I ACTGG 3 cut(s) 10, 370, 509
Bse21I CCTNAGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 318
BseBI CCWGG 2 cut(s) 294, 521
BseDI CCNNGG 3 cut(s) 293, 519, 528
BseGI GGATG 3 cut(s) 67, 463, 529
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 3 cut(s) 55, 90, 264
BseNI ACTGG 3 cut(s) 10, 370, 509
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 3 cut(s) 58, 61, 90
BshFI GGCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 699
BsiHKCI CYCGRG 2 cut(s) 258, 358
BsiSI CCGG 2 cut(s) 114, 131
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 595
BsnI GGCC 1 cut(s) 302
BsoBI CYCGRG 2 cut(s) 258, 358
Bsp1286I GDGCHC 2 cut(s) 269, 699
Bsp143I GATC 4 cut(s) 118, 127, 168, 735
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 1 cut(s) 743
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 3 cut(s) 54, 91, 263
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 612
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 122, 163
BsrDI GCAATG 1 cut(s) 318
BsrI ACTGG 3 cut(s) 10, 370, 509
BssECI CCNNGG 3 cut(s) 293, 519, 528
BssMI GATC 4 cut(s) 118, 127, 168, 735
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 2 cut(s) 294, 521
BstC8I GCNNGC 4 cut(s) 7, 210, 247, 347
BstDEI CTNAG 6 cut(s) 41, 99, 214, 250, 350, 656
BstF5I GGATG 3 cut(s) 67, 463, 529
BstKTI GATC 4 cut(s) 121, 130, 171, 738
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 4 cut(s) 118, 127, 168, 735
BstMWI GCNNNNNNNGC 3 cut(s) 109, 524, 578
BstNI CCWGG 2 cut(s) 294, 521
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 3 cut(s) 112, 292, 519
BstV1I GCAGC 3 cut(s) 58, 61, 90
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 214
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 3 cut(s) 67, 463, 529
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 2 cut(s) 49, 484
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 4 cut(s) 7, 210, 247, 347
CviAII CATG 6 cut(s) 157, 192, 246, 307, 560, 733
DdeI CTNAG 6 cut(s) 41, 99, 214, 250, 350, 656
DpnI GATC 4 cut(s) 120, 129, 170, 737
DpnII GATC 4 cut(s) 118, 127, 168, 735
DraI TTTAAA 1 cut(s) 289
EciI GGCGGA 1 cut(s) 732
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 528
Eco147I AGGCCT 1 cut(s) 302
Eco24I GRGCYC 2 cut(s) 269, 699
Eco53kI GAGCTC 1 cut(s) 697
Eco81I CCTNAGG 1 cut(s) 214
Eco88I CYCGRG 2 cut(s) 258, 358
EcoICRI GAGCTC 1 cut(s) 697
EcoRII CCWGG 2 cut(s) 292, 519
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 2 cut(s) 269, 699
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 6 cut(s) 160, 195, 249, 310, 563, 736
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 197, 229
FatI CATG 6 cut(s) 156, 191, 245, 306, 559, 732
FbaI TGATCA 2 cut(s) 118, 735
Fnu4HI GCNGC 3 cut(s) 72, 75, 104
FokI GGATG 3 cut(s) 74, 450, 536
FriOI GRGCYC 2 cut(s) 269, 699
Fsp4HI GCNGC 3 cut(s) 72, 75, 104
FspBI CTAG 2 cut(s) 6, 573
GluI GCNGC 3 cut(s) 72, 75, 104
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 1 cut(s) 302
HapII CCGG 2 cut(s) 114, 131
Hin1II CATG 6 cut(s) 160, 195, 249, 310, 563, 736
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 2 cut(s) 254, 713
HpaII CCGG 2 cut(s) 114, 131
HphI GGTGA 2 cut(s) 128, 717
Hpy188I TCNGA 2 cut(s) 253, 640
Hpy188III TCNNGA 5 cut(s) 166, 258, 360, 467, 596
HpyAV CCTTC 3 cut(s) 43, 313, 496
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 524, 578
HpyF3I CTNAG 6 cut(s) 41, 99, 214, 250, 350, 656
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 6 cut(s) 160, 195, 249, 310, 563, 736
Ksp22I TGATCA 2 cut(s) 118, 735
Kzo9I GATC 4 cut(s) 118, 127, 168, 735
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 3 cut(s) 58, 61, 90
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 6, 573
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 2 cut(s) 388, 665
MalI GATC 4 cut(s) 120, 129, 170, 737
MboI GATC 4 cut(s) 118, 127, 168, 735
MboII GAAGA 1 cut(s) 482
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 269, 699
MluCI AATT 8 cut(s) 18, 81, 93, 176, 230, 372, 513, 752
MlyI GAGTC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 35
MseI TTAA 5 cut(s) 282, 288, 510, 540, 755
MspI CCGG 2 cut(s) 114, 131
MspR9I CCNGG 3 cut(s) 114, 294, 521
MvaI CCWGG 2 cut(s) 294, 521
MwoI GCNNNNNNNGC 3 cut(s) 109, 524, 578
NciI CCSGG 1 cut(s) 114
NdeII GATC 4 cut(s) 118, 127, 168, 735
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 6 cut(s) 160, 195, 249, 310, 563, 736
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 258
PceI AGGCCT 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 713
PflFI GACNNNGTC 1 cut(s) 677
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 3 cut(s) 73, 76, 105
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 292, 519
PspGI CCWGG 2 cut(s) 292, 519
PspN4I GGNNCC 1 cut(s) 333
PsuI RGATCY 1 cut(s) 168
PsyI GACNNNGTC 1 cut(s) 677
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 5 cut(s) 282, 288, 510, 540, 755
SatI GCNGC 3 cut(s) 72, 75, 104
Sau3AI GATC 4 cut(s) 118, 127, 168, 735
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 3 cut(s) 114, 294, 521
SduI GDGCHC 2 cut(s) 269, 699
SfaNI GCATC 1 cut(s) 514
Sfr274I CTCGAG 1 cut(s) 258
SlaI CTCGAG 1 cut(s) 258
SmlI CTYRAG 2 cut(s) 258, 398
SmoI CTYRAG 2 cut(s) 258, 398
SphI GCATGC 1 cut(s) 249
Sse9I AATT 8 cut(s) 18, 81, 93, 176, 230, 372, 513, 752
SseBI AGGCCT 1 cut(s) 302
SsiI CCGC 1 cut(s) 743
SspMI CTAG 2 cut(s) 6, 573
SstI GAGCTC 1 cut(s) 699
StuI AGGCCT 1 cut(s) 302
StyD4I CCNGG 3 cut(s) 112, 292, 519
StyI CCWWGG 1 cut(s) 528
TaiI ACGT 1 cut(s) 624
TaqI TCGA 5 cut(s) 180, 201, 259, 489, 693
TasI AATT 8 cut(s) 18, 81, 93, 176, 230, 372, 513, 752
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 5 cut(s) 282, 288, 510, 540, 755
Tru9I TTAA 5 cut(s) 282, 288, 510, 540, 755
TscAI CASTG 2 cut(s) 49, 484
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 3 cut(s) 71, 74, 103
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 6 cut(s) 11, 78, 145, 295, 484, 614
TspRI CASTG 2 cut(s) 49, 484
Tth111I GACNNNGTC 1 cut(s) 677
Van91I CCANNNNNTGG 1 cut(s) 473
XapI RAATTY 2 cut(s) 176, 230
XceI RCATGY 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 2 cut(s) 6, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.