Rh6AG211200

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000548
Physical Location & Seq
Forward (+)
37656881 .. 37660791
3911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG211200.1

Sequence Viewer

Length: 735 bp
ATGATCGCGTGCAGTGAGAAGGAGGACTTGGATGAAGTGGCTGCTGCCAAAATTGACAAACTAATTGCTGAGCTGCAAACGCCCGGTGATCATTTTGATCCGGTTCAAACGATTGTAAATGGGTTCATGGACTTCAAGATCCACAAATTCGACAAATATCCATGTCTGTTCGACAAGCTTGCCTTAGGACAAAGCCCCAAATTTCTGGTGTTCGCATGCTCAGACTCTCGAGTGAGCCCCTCTATTATCCTTAACTTTAAACCTGGGGAGGCCTTCATGGTTCGCAACATTGCTAACTTGGTTCCTACATTTAGCCAGCTAAGATACTCGGGAACTGGAGCAATTATAGAATATGCCGTCACACAACTCAAGGTAGAAAACATTTTGGTTATTGGGCATAGTAAATGTGGTGGGATACAGAGGCTTATGGAACATCCTGAAGATGGCAGTGTTCCCTTCGACTTCATAGATGACTGGGTTAAAATTGCCCAGGATGCCAAGGATAAAGTTAAAGCAGAGGGAGGAGGACATGAGGCTTGTGCTAGGGAAGCTGTAAATGTCTCTCTGGAAAACCTGCTAACTTATCCTTACGTTCATAAGGAGGTGTCGGAAAGAAAAATAGCACTTAGAGGTGGTTACTATGACTTCGTCAATGGAGTTTTCGAGCTCTGGGAGAAACACGAATCTCACATTTCACCCCCCATGATCATTCCGCCTCCACAATTAAGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

244

Amino Acids

27.31

Weight (kDa)

5.5

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 69 - 219 4.7e-40 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01500 AT3G01500 AT3G01500 AT3G01500 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740 AT5G14740
fragaria_vesca FvH4_2g14190 FvH4_3g35690 FvH4_3g35690 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780 FvH4_6g50780
malus_domestica MD09G1032400.v1.1 MD10G1022000.v1.1 MD10G1022200.v1.1 MD10G1022400.v1.1 MD17G1034000.v1.1
prunus_persica Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.3G285600_v2.0.a1 Prupe.6G080400_v2.0.a1 Prupe.8G025400_v2.0.a1
pyrus_communis pycom10g01540 pycom111g02580 pycom17g03140
rosa_chinensis RchiOBHm_Chr2g0172091 RchiOBHm_Chr2g0172171 RchiOBHm_Chr5g0063841 RchiOBHm_Chr5g0063851 RchiOBHm_Chr6g0276331
rosa_laevigata RLG00000013403 RLG00000022084 RLG00000022089 RLG00000035683 RLG00000035684
rosa_multiflora Rmu_co8246953.1_g000001 Rmu_sc0002765.1_g000009 Rmu_sc0002765.1_g000011 Rmu_sc0003665.1_g000009 Rmu_ssc0000252.1_g000041 Rmu_ssc0000252.1_g000047
rosa_roxburghii Rroxscaffold_1G00016930 Rroxscaffold_2G00080110 Rroxscaffold_7G00192380
rosa_rugosa Rorug02G0556500 Rorug02G0556600 Rorug02G0556700 Rorug02G0556800 Rorug05G0360800 Rorug06G0100000 Rorug06G0100000
rosa_samantha Rh2AG632300 Rh2BG642200 Rh2BG645900 Rh2CG612500 Rh2DG653300 Rh2DG660500 Rh5AG418900 Rh5AG419000 Rh5BG435100 Rh5BG435200 Rh5CG458100 Rh5CG458200 Rh5DG447900 Rh5DG448000 Rh6AG211200 Rh6CG217900 Rh6DG208000
rosa_wichuraiana Rw2G052340 Rw5G039370 Rw5G039380 Rw6G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 582
AccII CGCG 1 cut(s) 8
AciI CCGC 1 cut(s) 713
AclWI GGATC 2 cut(s) 92, 133
AcsI RAATTY 2 cut(s) 146, 200
AcuI CTGAAG 1 cut(s) 459
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 2 cut(s) 107, 136
AjnI CCWGG 2 cut(s) 262, 489
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 5 cut(s) 73, 178, 319, 551, 667
AluI AGCT 5 cut(s) 73, 178, 319, 551, 667
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 2 cut(s) 92, 133
Ama87I CYCGRG 2 cut(s) 228, 328
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 3 cut(s) 41, 44, 73
ApoI RAATTY 2 cut(s) 146, 200
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 98, 687
AvaI CYCGRG 2 cut(s) 228, 328
AxyI CCTNAGG 1 cut(s) 184
BanII GRGCYC 2 cut(s) 239, 669
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 28, 31, 60
BccI CCATC 1 cut(s) 437
BceAI ACGGC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 2 cut(s) 264, 491
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 2 cut(s) 88, 705
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 582
BfuI GTATCC 1 cut(s) 408
BisI GCNGC 3 cut(s) 42, 45, 74
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 3 cut(s) 43, 46, 75
Bme1390I CCNGG 3 cut(s) 84, 264, 491
BmeT110I CYCGRG 2 cut(s) 228, 328
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 3 cut(s) 84, 264, 491
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 1 cut(s) 484
BmuI ACTGGG 1 cut(s) 484
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 263, 489, 498
BsaWI WCCGGW 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 340, 479
Bse21I CCTNAGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 2 cut(s) 264, 491
BseDI CCNNGG 3 cut(s) 263, 489, 498
BseGI GGATG 4 cut(s) 37, 433, 499, 735
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 2 cut(s) 60, 234
BseNI ACTGG 2 cut(s) 340, 479
BseRI GAGGAG 1 cut(s) 537
BseXI GCAGC 3 cut(s) 28, 31, 60
BsgI GTGCAG 1 cut(s) 31
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 2 cut(s) 228, 328
BsiSI CCGG 2 cut(s) 84, 101
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 565
BsnI GGCC 1 cut(s) 272
BsoBI CYCGRG 2 cut(s) 228, 328
Bsp1286I GDGCHC 2 cut(s) 239, 669
Bsp143I GATC 5 cut(s) 3, 88, 97, 138, 705
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 1 cut(s) 713
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 2 cut(s) 61, 233
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 303
BspMI ACCTGC 1 cut(s) 582
BspPI GGATC 2 cut(s) 92, 133
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 2 cut(s) 340, 479
BssECI CCNNGG 3 cut(s) 263, 489, 498
BssMI GATC 5 cut(s) 3, 88, 97, 138, 705
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 2 cut(s) 264, 491
BstC8I GCNNGC 4 cut(s) 10, 180, 217, 317
BstDEI CTNAG 5 cut(s) 69, 184, 220, 320, 626
BstF5I GGATG 4 cut(s) 37, 433, 499, 735
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 5 cut(s) 6, 91, 100, 141, 708
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 5 cut(s) 3, 88, 97, 138, 705
BstMWI GCNNNNNNNGC 3 cut(s) 79, 494, 548
BstNI CCWGG 2 cut(s) 264, 491
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 3 cut(s) 82, 262, 489
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 28, 31, 60
BstX2I RGATCY 1 cut(s) 138
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 138
Bsu36I CCTNAGG 1 cut(s) 184
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 272
BtsCI GGATG 4 cut(s) 37, 433, 499, 735
BtsI GCAGTG 2 cut(s) 19, 454
BtsIMutI CAGTG 2 cut(s) 19, 454
BveI ACCTGC 1 cut(s) 582
Cac8I GCNNGC 4 cut(s) 10, 180, 217, 317
CviAII CATG 6 cut(s) 127, 162, 216, 277, 530, 703
DdeI CTNAG 5 cut(s) 69, 184, 220, 320, 626
DpnI GATC 5 cut(s) 5, 90, 99, 140, 707
DpnII GATC 5 cut(s) 3, 88, 97, 138, 705
DraI TTTAAA 1 cut(s) 259
EciI GGCGGA 1 cut(s) 702
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 498
Eco147I AGGCCT 1 cut(s) 272
Eco24I GRGCYC 2 cut(s) 239, 669
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 459
Eco81I CCTNAGG 1 cut(s) 184
Eco88I CYCGRG 2 cut(s) 228, 328
EcoICRI GAGCTC 1 cut(s) 667
EcoRII CCWGG 2 cut(s) 262, 489
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 2 cut(s) 239, 669
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 6 cut(s) 130, 165, 219, 280, 533, 706
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 167, 199
FatI CATG 6 cut(s) 126, 161, 215, 276, 529, 702
FbaI TGATCA 2 cut(s) 88, 705
Fnu4HI GCNGC 3 cut(s) 42, 45, 74
FokI GGATG 3 cut(s) 44, 420, 506
FriOI GRGCYC 2 cut(s) 239, 669
Fsp4HI GCNGC 3 cut(s) 42, 45, 74
FspBI CTAG 1 cut(s) 543
GluI GCNGC 3 cut(s) 42, 45, 74
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 272
HapII CCGG 2 cut(s) 84, 101
Hin1II CATG 6 cut(s) 130, 165, 219, 280, 533, 706
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 2 cut(s) 224, 683
HpaII CCGG 2 cut(s) 84, 101
HphI GGTGA 2 cut(s) 98, 687
Hpy188I TCNGA 2 cut(s) 223, 610
Hpy188III TCNNGA 5 cut(s) 136, 228, 330, 437, 566
HpyAV CCTTC 3 cut(s) 13, 283, 466
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 2 cut(s) 12, 76
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 494, 548
HpyF3I CTNAG 5 cut(s) 69, 184, 220, 320, 626
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 6 cut(s) 130, 165, 219, 280, 533, 706
Ksp22I TGATCA 2 cut(s) 88, 705
Kzo9I GATC 5 cut(s) 3, 88, 97, 138, 705
LmnI GCTCC 1 cut(s) 338
Lsp1109I GCAGC 3 cut(s) 28, 31, 60
LweI GCATC 1 cut(s) 484
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 358, 635
MalI GATC 5 cut(s) 5, 90, 99, 140, 707
MboI GATC 5 cut(s) 3, 88, 97, 138, 705
MboII GAAGA 1 cut(s) 452
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 239, 669
MluCI AATT 7 cut(s) 51, 63, 146, 200, 342, 483, 722
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 588
MseI TTAA 5 cut(s) 252, 258, 480, 510, 725
MspI CCGG 2 cut(s) 84, 101
MspR9I CCNGG 3 cut(s) 84, 264, 491
MvaI CCWGG 2 cut(s) 264, 491
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 3 cut(s) 79, 494, 548
NciI CCSGG 1 cut(s) 84
NdeII GATC 5 cut(s) 3, 88, 97, 138, 705
NlaIII CATG 6 cut(s) 130, 165, 219, 280, 533, 706
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 358
NspI RCATGY 1 cut(s) 219
PaeI GCATGC 1 cut(s) 219
PaeR7I CTCGAG 1 cut(s) 228
PceI AGGCCT 1 cut(s) 272
PfeI GAWTC 1 cut(s) 683
PflFI GACNNNGTC 1 cut(s) 647
PkrI GCNGC 3 cut(s) 43, 46, 75
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 262, 489
PspGI CCWGG 2 cut(s) 262, 489
PspN4I GGNNCC 1 cut(s) 303
PsuI RGATCY 1 cut(s) 138
PsyI GACNNNGTC 1 cut(s) 647
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 5 cut(s) 252, 258, 480, 510, 725
SatI GCNGC 3 cut(s) 42, 45, 74
Sau3AI GATC 5 cut(s) 3, 88, 97, 138, 705
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 3 cut(s) 84, 264, 491
SduI GDGCHC 2 cut(s) 239, 669
SfaNI GCATC 1 cut(s) 484
Sfr274I CTCGAG 1 cut(s) 228
SlaI CTCGAG 1 cut(s) 228
SmlI CTYRAG 2 cut(s) 228, 368
SmoI CTYRAG 2 cut(s) 228, 368
SphI GCATGC 1 cut(s) 219
Sse9I AATT 7 cut(s) 51, 63, 146, 200, 342, 483, 722
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 1 cut(s) 713
SspMI CTAG 1 cut(s) 543
SstI GAGCTC 1 cut(s) 669
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 82, 262, 489
StyI CCWWGG 1 cut(s) 498
TaiI ACGT 1 cut(s) 594
TaqI TCGA 5 cut(s) 150, 171, 229, 459, 663
TasI AATT 7 cut(s) 51, 63, 146, 200, 342, 483, 722
TfiI GAWTC 1 cut(s) 683
Tru1I TTAA 5 cut(s) 252, 258, 480, 510, 725
Tru9I TTAA 5 cut(s) 252, 258, 480, 510, 725
TscAI CASTG 2 cut(s) 19, 454
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 3 cut(s) 41, 44, 73
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 5 cut(s) 48, 115, 265, 454, 584
TspRI CASTG 2 cut(s) 19, 454
Tth111I GACNNNGTC 1 cut(s) 647
XapI RAATTY 2 cut(s) 146, 200
XceI RCATGY 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 228
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.