FvH4_4g13212

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000894
Physical Location & Seq
Reverse (-)
16813832 .. 16816545
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13212.t1

Sequence Viewer

Length: 1458 bp
ATGGAGAGAATACTTACCGGAAAGTGTTCTCGAGAAGTCTTGCTTGGAATACTAGTGTCATTTTGTTTATGCTTTGCATTGCTTTTCCTCCACCATTCAACTCCATTGCGTTTCAGCAATTGGGTTAACATTTTAGGTAACAGACATGTTCACAACATATTTGATTCTTGCTCCGGCCGTTACATTTATGTCTATGATGATCTTCCTGCCAAATTCAACTATGACCTGCTCAGCAACTGCAAGTCACTTAGTTCAGGAACTACTAGTGATTCTGATGACAATAAAAATATGTGTCCTTACCTTGTAAATATGGGTCTTGGTCCTGAAATTGAGAGCTCCAGAGGTGTTTTAGCGAACAAGAGTTGGTTTTCCACAAACCCCTACTTGTTAGAAGTCATATTCCACAACAGGATGAAGCAGTATGAGTGCTTGACAAACATCTCCACACTAGCTTCAGCAATTTATGTACCATACTACACTGGCCTGGACGCCAGTCTCCATCTTTGGGATACTAATCTAACAGTCAGAGATGCATCTGCTAAAGATCTACTCAAGTGGATTTCAGGGAAATCTGAATGGAAGAGAATGTGGGGCAGAGACCATTTCATGGTGACTGGCAGGACTTCATGGGATTTCAGGAGGACTAATGATAGCTCGGAATGGCCATGGGGCAGTTTTCTCAGGTTCTTGCCAGAGTCGAAGAACATGAGTATGCTTTCGGTTGAAGGAAGCTTGTGGAAAAATGACTTTGCTATTCCATACCCCACAAACTTTCATCCTGCAAAGGATAGTGAAATTATTCAGTGGCAGAACAGAATGAGATATCTGCAAAGGCCTTACTTATTTGCTTTTGTTGGTGCGCCAAGGCCTGGCCTGGAAAAATCTATCAGGGGGAACCTCATTGATCAGTGCCGAGCTTCAAGCACTTGCAAGTTTGTACATTGTGGTTCTAAAGGGGTAAATTGCAACAACCCGGTTACTGTTATGAAGGTGTTTCAGAGCTCAGTATTTTGCTTGCAGCCTCCAGGGGATTCACACACTAGGAGATCAGCTTTTGACTCTATGCTGGCTGGTTGTATTCCTGTTTTCTTCAATCCAGGTTCAGCTTATAACCAATATTTATGGCATTTACCAGATAATCATAGCACATATTCGGTGTTTATCCCGGCAAAGGATGTAAATGATTTGAAAAATGTCTCCATAGAGAAACGTTTGCTTGGTATTTCAAAGGAGAAGCAATTGGAAATGAGAACGAAGGTCATTAAATTAATACCAAAGCTGGTATATGCAGATCCCAGGTCAAGCTTAGTGACTGAAGATGCATTTGATTTTGCTGTTATGGGAATTCTTGAGAGAATAGAAAATGTTAGGCAGTTGATTAGAGAGGGAAAAGATCCTAGTATTGGTTTCGCAGATGAAGATAGTGTAAAATACACATTTCCTGAGACACTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

486

Amino Acids

55.49

Weight (kDa)

8.45

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 56 - 399 1.7e-81 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1110
Acc36I ACCTGC 1 cut(s) 234
AccB7I CCANNNNNTGG 2 cut(s) 607, 869
AclI AACGTT 1 cut(s) 1210
AclWI GGATC 2 cut(s) 1286, 1388
AcoI YGGCCR 2 cut(s) 175, 662
AcsI RAATTY 2 cut(s) 212, 1344
AcuI CTGAAG 2 cut(s) 438, 1335
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 2 cut(s) 468, 939
AfiI CCNNNNNNNGG 5 cut(s) 505, 607, 869, 1171, 1403
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 7 cut(s) 99, 217, 725, 921, 1093, 1189, 1227
AhlI ACTAGT 2 cut(s) 52, 263
AjnI CCWGG 6 cut(s) 483, 868, 873, 1024, 1096, 1295
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
Alw21I GWGCWC 2 cut(s) 338, 1004
Alw26I GTCTC 4 cut(s) 500, 591, 1201, 1439
AlwI GGATC 2 cut(s) 1286, 1388
AlwNI CAGNNNCTG 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 6 cut(s) 175, 481, 662, 833, 866, 871
ApeKI GCWGC 1 cut(s) 1018
ApoI RAATTY 2 cut(s) 212, 1344
AseI ATTAAT 1 cut(s) 1268
Asp700I GAANNNNTTC 2 cut(s) 25, 798
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 2 cut(s) 974, 1166
AsuHPI GGTGA 1 cut(s) 622
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 664
BanII GRGCYC 2 cut(s) 338, 1004
Bbv12I GWGCWC 2 cut(s) 338, 1004
BbvI GCAGC 1 cut(s) 1030
BccI CCATC 1 cut(s) 507
BceAI ACGGC 1 cut(s) 162
BciT130I CCWGG 6 cut(s) 485, 870, 875, 1026, 1098, 1297
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 1 cut(s) 904
BcnI CCSGG 2 cut(s) 974, 1166
BcoDI GTCTC 4 cut(s) 500, 591, 1201, 1439
BcuI ACTAGT 2 cut(s) 52, 263
BfaI CTAG 6 cut(s) 53, 264, 449, 1041, 1398, 1451
BfuAI ACCTGC 1 cut(s) 234
BfuI GTATCC 1 cut(s) 502
BglII AGATCT 1 cut(s) 544
BisI GCNGC 1 cut(s) 1019
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 1020
Bme1390I CCNGG 8 cut(s) 485, 870, 875, 974, 1026, 1098, 1166, 1297
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 896
BmrFI CCNGG 8 cut(s) 485, 870, 875, 974, 1026, 1098, 1166, 1297
BmsI GCATC 3 cut(s) 520, 542, 1309
BoxI GACNNNNGTC 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 322, 1008
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 2 cut(s) 536, 1370
BpuMI CCSGG 2 cut(s) 974, 1166
BsaBI GATNNNNATC 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 591
BsaJI CCNNGG 4 cut(s) 665, 863, 1025, 1295
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 5 cut(s) 505, 607, 869, 1171, 1403
Bse1I ACTGG 3 cut(s) 484, 492, 619
Bse3DI GCAATG 2 cut(s) 77, 104
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 6 cut(s) 485, 870, 875, 1026, 1098, 1297
BseDI CCNNGG 4 cut(s) 665, 863, 1025, 1295
BseGI GGATG 3 cut(s) 417, 775, 1180
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 5 cut(s) 505, 607, 869, 1171, 1403
BseMI GCAATG 2 cut(s) 77, 104
BseMII CTCAG 4 cut(s) 244, 694, 1017, 1434
BseNI ACTGG 3 cut(s) 484, 492, 619
BseX3I CGGCCG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 1030
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 6 cut(s) 177, 483, 664, 835, 868, 873
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 2 cut(s) 338, 1004
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 4 cut(s) 18, 174, 974, 1166
BslI CCNNNNNNNGG 5 cut(s) 505, 607, 869, 1171, 1403
BsmAI GTCTC 4 cut(s) 500, 591, 1201, 1439
BsnI GGCC 6 cut(s) 177, 483, 664, 835, 868, 873
Bso31I GGTCTC 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 338, 1004
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
Bsp1720I GCTNAGC 1 cut(s) 230
Bsp19I CCATGG 1 cut(s) 665
BspANI GGCC 6 cut(s) 177, 483, 664, 835, 868, 873
BspCNI CTCAG 4 cut(s) 243, 693, 1016, 1435
BspLI GGNNCC 1 cut(s) 896
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 2 cut(s) 1286, 1388
BspTNI GGTCTC 1 cut(s) 591
BsrDI GCAATG 2 cut(s) 77, 104
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 3 cut(s) 484, 492, 619
BssECI CCNNGG 4 cut(s) 665, 863, 1025, 1295
BssMI GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
BssNI GRCGYC 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 665, 863
Bst2UI CCWGG 6 cut(s) 485, 870, 875, 1026, 1098, 1297
Bst4CI ACNGT 2 cut(s) 523, 982
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 1 cut(s) 489
BstAUI TGTACA 1 cut(s) 937
BstC8I GCNNGC 2 cut(s) 1016, 1068
BstDEI CTNAG 6 cut(s) 230, 248, 680, 1003, 1306, 1443
BstDSI CCRYGG 1 cut(s) 665
BstF5I GGATG 3 cut(s) 417, 775, 1180
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 6 cut(s) 202, 547, 907, 1049, 1294, 1396
BstMAI GTCTC 4 cut(s) 500, 591, 1201, 1439
BstMBI GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
BstMCI CGRYCG 1 cut(s) 178
BstNI CCWGG 6 cut(s) 485, 870, 875, 1026, 1098, 1297
BstNSI RCATGY 1 cut(s) 149
BstPAI GACNNNNGTC 1 cut(s) 492
BstSCI CCNGG 8 cut(s) 483, 868, 873, 972, 1024, 1096, 1164, 1295
BstV1I GCAGC 1 cut(s) 1030
BstX2I RGATCY 3 cut(s) 544, 1291, 1393
BstYI RGATCY 3 cut(s) 544, 1291, 1393
BstZI CGGCCG 1 cut(s) 175
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 6 cut(s) 177, 483, 664, 835, 868, 873
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 3 cut(s) 417, 775, 1180
BtsIMutI CAGTG 3 cut(s) 477, 809, 914
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 1016, 1068
CaiI CAGNNNCTG 1 cut(s) 237
CfoI GCGC 1 cut(s) 862
Cfr13I GGNCC 1 cut(s) 320
CseI GACGC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 467, 938
CviAII CATG 5 cut(s) 146, 607, 627, 666, 706
CviQI GTAC 2 cut(s) 467, 938
DdeI CTNAG 6 cut(s) 230, 248, 680, 1003, 1306, 1443
DpnI GATC 6 cut(s) 201, 546, 906, 1048, 1293, 1395
DpnII GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
EaeI YGGCCR 2 cut(s) 175, 662
EagI CGGCCG 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Ecl136II GAGCTC 2 cut(s) 336, 1002
EclXI CGGCCG 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 665, 863
Eco147I AGGCCT 2 cut(s) 835, 868
Eco24I GRGCYC 2 cut(s) 338, 1004
Eco31I GGTCTC 1 cut(s) 591
Eco32I GATATC 1 cut(s) 824
Eco47I GGWCC 1 cut(s) 320
Eco52I CGGCCG 1 cut(s) 175
Eco53kI GAGCTC 2 cut(s) 336, 1002
Eco57I CTGAAG 2 cut(s) 438, 1335
Eco88I CYCGRG 1 cut(s) 30
EcoICRI GAGCTC 2 cut(s) 336, 1002
EcoRI GAATTC 1 cut(s) 1344
EcoRII CCWGG 6 cut(s) 483, 868, 873, 1024, 1096, 1295
EcoRV GATATC 1 cut(s) 824
EcoT14I CCWWGG 2 cut(s) 665, 863
EcoT22I ATGCAT 2 cut(s) 535, 1324
EcoT38I GRGCYC 2 cut(s) 338, 1004
ErhI CCWWGG 2 cut(s) 665, 863
FaeI CATG 5 cut(s) 149, 610, 630, 669, 709
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FatI CATG 5 cut(s) 145, 606, 626, 665, 705
FbaI TGATCA 1 cut(s) 904
Fnu4HI GCNGC 1 cut(s) 1019
FokI GGATG 3 cut(s) 424, 762, 1187
FriOI GRGCYC 2 cut(s) 338, 1004
Fsp4HI GCNGC 1 cut(s) 1019
FspBI CTAG 6 cut(s) 53, 264, 449, 1041, 1398, 1451
GlaI GCGC 1 cut(s) 861
GluI GCNGC 1 cut(s) 1019
GsuI CTGGAG 2 cut(s) 322, 1008
HaeIII GGCC 6 cut(s) 177, 483, 664, 835, 868, 873
HapII CCGG 4 cut(s) 18, 174, 974, 1166
HgaI GACGC 1 cut(s) 497
HhaI GCGC 1 cut(s) 862
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 5 cut(s) 149, 610, 630, 669, 709
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 730, 1303
HinfI GANTC 5 cut(s) 164, 269, 695, 1031, 1058
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 4 cut(s) 18, 174, 974, 1166
HphI GGTGA 1 cut(s) 622
Hpy166II GTNNAC 2 cut(s) 127, 151
Hpy188I TCNGA 5 cut(s) 274, 527, 574, 658, 999
Hpy188III TCNNGA 8 cut(s) 30, 32, 255, 323, 339, 637, 1349, 1442
Hpy8I GTNNAC 2 cut(s) 127, 151
HpyAV CCTTC 3 cut(s) 719, 982, 1249
HpyCH4III ACNGT 2 cut(s) 523, 982
HpyCH4IV ACGT 1 cut(s) 1210
HpyF3I CTNAG 6 cut(s) 230, 248, 680, 1003, 1306, 1443
HpySE526I ACGT 1 cut(s) 1210
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 5 cut(s) 149, 610, 630, 669, 709
HspAI GCGC 1 cut(s) 860
Ksp22I TGATCA 1 cut(s) 904
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
LmnI GCTCC 2 cut(s) 176, 341
Lsp1109I GCAGC 1 cut(s) 1030
LweI GCATC 3 cut(s) 520, 542, 1309
MaeI CTAG 6 cut(s) 53, 264, 449, 1041, 1398, 1451
MaeII ACGT 1 cut(s) 1210
MaeIII GTNAC 6 cut(s) 137, 179, 243, 610, 976, 1309
MalI GATC 6 cut(s) 201, 546, 906, 1048, 1293, 1395
MboI GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
MboII GAAGA 6 cut(s) 194, 592, 712, 1081, 1328, 1430
MfeI CAATTG 2 cut(s) 118, 1238
MflI RGATCY 3 cut(s) 544, 1291, 1393
MhlI GDGCHC 2 cut(s) 338, 1004
MlsI TGGCCA 1 cut(s) 664
MluCI AATT 9 cut(s) 118, 212, 327, 459, 795, 961, 1238, 1265, 1344
MluNI TGGCCA 1 cut(s) 664
MlyI GAGTC 2 cut(s) 704, 1052
MnlI CCTC 6 cut(s) 98, 335, 633, 908, 1032, 1378
Mox20I TGGCCA 1 cut(s) 664
Mph1103I ATGCAT 2 cut(s) 535, 1324
MroXI GAANNNNTTC 2 cut(s) 25, 798
MscI TGGCCA 1 cut(s) 664
MseI TTAA 3 cut(s) 126, 1263, 1268
MslI CAYNNNNRTG 1 cut(s) 710
Msp20I TGGCCA 1 cut(s) 664
MspI CCGG 4 cut(s) 18, 174, 974, 1166
MspR9I CCNGG 8 cut(s) 485, 870, 875, 974, 1026, 1098, 1166, 1297
MunI CAATTG 2 cut(s) 118, 1238
MvaI CCWGG 6 cut(s) 485, 870, 875, 1026, 1098, 1297
NciI CCSGG 2 cut(s) 974, 1166
NcoI CCATGG 1 cut(s) 665
NdeII GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
NlaIII CATG 5 cut(s) 149, 610, 630, 669, 709
NlaIV GGNNCC 1 cut(s) 896
NmeAIII GCCGAG 1 cut(s) 938
NmuCI GTSAC 3 cut(s) 243, 610, 1309
NsiI ATGCAT 2 cut(s) 535, 1324
NspI RCATGY 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 30
PceI AGGCCT 2 cut(s) 835, 868
PciI ACATGT 1 cut(s) 145
PdmI GAANNNNTTC 2 cut(s) 25, 798
PfeI GAWTC 3 cut(s) 164, 269, 1031
PflMI CCANNNNNTGG 2 cut(s) 607, 869
PkrI GCNGC 1 cut(s) 1020
PleI GAGTC 2 cut(s) 703, 1052
PpsI GAGTC 2 cut(s) 703, 1052
PscI ACATGT 1 cut(s) 145
PshAI GACNNNNGTC 1 cut(s) 492
PshBI ATTAAT 1 cut(s) 1268
PsiI TTATAA 1 cut(s) 1110
Psp124BI GAGCTC 2 cut(s) 338, 1004
Psp1406I AACGTT 1 cut(s) 1210
Psp6I CCWGG 6 cut(s) 483, 868, 873, 1024, 1096, 1295
PspGI CCWGG 6 cut(s) 483, 868, 873, 1024, 1096, 1295
PspN4I GGNNCC 1 cut(s) 896
PspPI GGNCC 1 cut(s) 320
PstNI CAGNNNCTG 1 cut(s) 237
PsuI RGATCY 3 cut(s) 544, 1291, 1393
RsaI GTAC 2 cut(s) 468, 939
RsaNI GTAC 2 cut(s) 467, 938
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 2 cut(s) 338, 1004
SaqAI TTAA 3 cut(s) 126, 1263, 1268
SatI GCNGC 1 cut(s) 1019
Sau3AI GATC 6 cut(s) 199, 544, 904, 1046, 1291, 1393
Sau96I GGNCC 1 cut(s) 320
SchI GAGTC 2 cut(s) 704, 1052
ScrFI CCNGG 8 cut(s) 485, 870, 875, 974, 1026, 1098, 1166, 1297
SduI GDGCHC 2 cut(s) 338, 1004
SfaNI GCATC 3 cut(s) 520, 542, 1309
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 3 cut(s) 30, 551, 1349
SmoI CTYRAG 3 cut(s) 30, 551, 1349
SpeI ACTAGT 2 cut(s) 52, 263
Sse9I AATT 9 cut(s) 118, 212, 327, 459, 795, 961, 1238, 1265, 1344
SseBI AGGCCT 2 cut(s) 835, 868
SspI AATATT 1 cut(s) 1118
SspMI CTAG 6 cut(s) 53, 264, 449, 1041, 1398, 1451
SstI GAGCTC 2 cut(s) 338, 1004
StuI AGGCCT 2 cut(s) 835, 868
StyD4I CCNGG 8 cut(s) 483, 868, 873, 972, 1024, 1096, 1164, 1295
StyI CCWWGG 2 cut(s) 665, 863
TaaI ACNGT 2 cut(s) 523, 982
TaiI ACGT 1 cut(s) 1213
TaqI TCGA 2 cut(s) 31, 698
TasI AATT 9 cut(s) 118, 212, 327, 459, 795, 961, 1238, 1265, 1344
TatI WGTACW 1 cut(s) 937
TfiI GAWTC 3 cut(s) 164, 269, 1031
Tru1I TTAA 3 cut(s) 126, 1263, 1268
Tru9I TTAA 3 cut(s) 126, 1263, 1268
TscAI CASTG 3 cut(s) 484, 809, 914
TseFI GTSAC 3 cut(s) 243, 610, 1309
TseI GCWGC 1 cut(s) 1018
Tsp45I GTSAC 3 cut(s) 243, 610, 1309
TspDTI ATGAA 6 cut(s) 428, 595, 615, 764, 1001, 1431
TspRI CASTG 3 cut(s) 484, 809, 914
Van91I CCANNNNNTGG 2 cut(s) 607, 869
VpaK11BI GGWCC 1 cut(s) 320
VspI ATTAAT 1 cut(s) 1268
XapI RAATTY 2 cut(s) 212, 1344
XceI RCATGY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 30
XmnI GAANNNNTTC 2 cut(s) 25, 798
XspI CTAG 6 cut(s) 53, 264, 449, 1041, 1398, 1451
Zsp2I ATGCAT 2 cut(s) 535, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.