pycom420g00910

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0000894
Physical Location & Seq
Reverse (-)
1207455 .. 1208933
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00910.1

Sequence Viewer

Length: 1479 bp
ATGGAGAAACTACTCGCCGGAAAGTGCTACAGGGAAGTCTGGTTTGGTGTACTAGTCTCATTTTTCTTGTTCTTTGTATTGCTTTGCTTTGATTATTCAACTTCAATATTGGATGTCTACCATGGGGTCAATTTTTCAGTCATTCACCATGTTCGTCCATTCTACATCAAAGATTCGTGTTTCGGCCGCTACATTTACATCCACGATCATCTTCCCGCACGGTTCAACTTTGATCTCCTCGCAAACTGCCATTCTCTTTCGACAGGGAGCAAGTCCCTTAGTGATCAAGGTGCTAATACTAGTGACAATATGTGTCCTCACCTTGTGAATTCGGGTCTTGGTCCTCAAATCGAAAAACCCGGAAAGGTTTTGGCAAACAAGAGTTGGTTCCTCACCAATCCCTACTTGTTAGAAGTTATATTCCACAACAAGATGAAGACGTACAAATGCTTGACGAACAACTCCAATCTGGCTTCGGCCATTTATGTACCGTCCTATACCGGCCTCGATGCAAGTCTCCATCTGTGGGATTTTGATCTCAAAGTCAGGGACGCTTCTGCGAAAGATCTTGTGAATTGGCTTTCGAGGAGGCCCGAGTGGAAGAAAATGTGGGGCAGAGATCATTTTTTTGTTACCGGGAGGACTTCTTGGGATTTCAGGAGGACCTCGGACAATGGTTCTGATTGGGGTACTAATTTGAGGTTTTTGCCGGAATCGTTGAACATGAGTATGTTGTCGGTTGAAGGAAGTTGTTGGAGAAATGACCTTGCTATACCGTACCCGACAAACTTTCATCCTGCGAGCGACAGTGAGGTTGTTCAATGGCAGAACAGAGTGAGGAAGTACAAAAGGCCTCACTTGTTCTCTTTTGTGGGCGCGCCGCGGCCTGGCATGCAAAATTCCATTCGGGGCAAACTCATTGATCAATGCCAAGCTTCGAGTAATTGCAAATTCGTGCATTGTGGTTCTCCTGGGATTAATTGTAACGACCCGGTTACGGTTATGAGGGTTTTCGAGAAGTCGGTCTATTGCTTGCAGCCTCCGGGGGACTCACATACTAGGAGATCAGCATTTGATTCTTTACTGGCTGGTTGTATTCCGGTCTTCTTCCATCCTGCGACTGCGTACACGCAGTACTTATGGCATTTGCCGGAGAAACATACCAAGTATTCGGTGTTTATTCCGGTGAAGGAAGTGGAAGATTTGAAACAAGGCATGATTGAAAAAACATTGCTTCGAATTTCAAATGAGAAGGCATTGGCAATGAGGGAGGAGGTCATAAGGCTGGTACCAAAGCTGGTGTACGCAGATCCTAGGTCGAGATTGGAGAGCGAAGACGCGTTTGATTCAGCTGTACAGGGAGTTCTTGAGAGGATAGAGAATGTTAGGAAGGTGATTAGAGAAGGGAACGATCCTAGCACTGGTTTTGCAGATGAGGATTGGACTAAATTCATGTTTGCTGAGGCGCCAGAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

493

Amino Acids

56.41

Weight (kDa)

8.31

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 60 - 403 9.2e-76 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1288
AccB1I GGYRCC 2 cut(s) 1288, 1465
AccI GTMKAC 1 cut(s) 117
AccII CGCG 3 cut(s) 878, 883, 1340
AciI CCGC 4 cut(s) 187, 216, 881, 883
AclWI GGATC 2 cut(s) 1304, 1406
AcoI YGGCCR 2 cut(s) 184, 477
AcsI RAATTY 5 cut(s) 328, 898, 950, 1239, 1448
AcyI GRCGYC 1 cut(s) 1466
AdeI CACNNNGTG 1 cut(s) 325
AfiI CCNNNNNNNGG 4 cut(s) 526, 887, 997, 1421
AflIII ACRYGT 1 cut(s) 1338
AgsI TTSAA 9 cut(s) 99, 105, 226, 721, 743, 821, 1207, 1223, 1245
AhlI ACTAGT 2 cut(s) 52, 299
AjnI CCWGG 2 cut(s) 886, 970
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 3 cut(s) 935, 1297, 1352
AluI AGCT 3 cut(s) 935, 1297, 1352
Alw26I GTCTC 2 cut(s) 61, 521
AlwI GGATC 2 cut(s) 1304, 1406
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 6 cut(s) 184, 477, 502, 590, 851, 884
ApeKI GCWGC 1 cut(s) 1036
ApoI RAATTY 5 cut(s) 328, 898, 950, 1239, 1448
AscI GGCGCGCC 1 cut(s) 876
AseI ATTAAT 1 cut(s) 978
Asp718I GGTACC 1 cut(s) 1288
AspA2I CCTAGG 1 cut(s) 1313
AspLEI GCGC 3 cut(s) 878, 880, 1468
AspS9I GGNCC 3 cut(s) 341, 591, 663
AsuC2I CCSGG 4 cut(s) 360, 637, 992, 1044
AsuHPI GGTGA 5 cut(s) 137, 311, 385, 1198, 1405
AsuII TTCGAA 1 cut(s) 1237
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 2 cut(s) 341, 663
AvrII CCTAGG 1 cut(s) 1313
BanI GGYRCC 2 cut(s) 1288, 1465
BbsI GAAGAC 3 cut(s) 443, 1096, 1341
BbvCI CCTCAGC 1 cut(s) 1461
BbvI GCAGC 1 cut(s) 1048
BccI CCATC 2 cut(s) 528, 1119
BciT130I CCWGG 2 cut(s) 888, 972
BclI TGATCA 2 cut(s) 283, 922
BcnI CCSGG 4 cut(s) 360, 637, 992, 1044
BcoDI GTCTC 2 cut(s) 61, 521
BcuI ACTAGT 2 cut(s) 52, 299
BfaI CTAG 5 cut(s) 53, 300, 1059, 1314, 1416
BfmI CTRYAG 1 cut(s) 28
BfoI RGCGCY 1 cut(s) 1469
BglII AGATCT 1 cut(s) 565
BisI GCNGC 4 cut(s) 187, 881, 884, 1037
BlnI CCTAGG 1 cut(s) 1313
BlsI GCNGC 4 cut(s) 188, 882, 885, 1038
BmcAI AGTACT 1 cut(s) 1136
Bme1390I CCNGG 6 cut(s) 360, 637, 888, 972, 992, 1044
Bme18I GGWCC 2 cut(s) 341, 663
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 3 cut(s) 341, 591, 663
BmiI GGNNCC 3 cut(s) 389, 1290, 1467
BmrFI CCNGG 6 cut(s) 360, 637, 888, 972, 992, 1044
BmsI GCATC 1 cut(s) 499
BpiI GAAGAC 3 cut(s) 443, 1096, 1341
Bpu10I CCTNAGC 1 cut(s) 1461
Bpu14I TTCGAA 1 cut(s) 1237
BpuEI CTTGAG 1 cut(s) 1388
BpuMI CCSGG 4 cut(s) 360, 637, 992, 1044
BsaBI GATNNNNATC 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 1466
BsaJI CCNNGG 6 cut(s) 121, 666, 881, 971, 1043, 1313
BsaWI WCCGGW 2 cut(s) 1099, 1183
Bsc4I CCNNNNNNNGG 4 cut(s) 526, 887, 997, 1421
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 2 cut(s) 1089, 1426
Bse3DI GCAATG 2 cut(s) 1229, 1269
Bse8I GATNNNNATC 1 cut(s) 534
BseBI CCWGG 2 cut(s) 888, 972
BseDI CCNNGG 6 cut(s) 121, 666, 881, 971, 1043, 1313
BseGI GGATG 4 cut(s) 118, 198, 793, 1111
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 4 cut(s) 526, 887, 997, 1421
BseMI GCAATG 2 cut(s) 1229, 1269
BseMII CTCAG 1 cut(s) 1452
BseNI ACTGG 2 cut(s) 1089, 1426
BsePI GCGCGC 1 cut(s) 876
BseRI GAGGAG 3 cut(s) 227, 601, 1286
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 1048
Bsh1236I CGCG 3 cut(s) 878, 883, 1340
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 6 cut(s) 186, 479, 504, 592, 853, 886
BshNI GGYRCC 2 cut(s) 1288, 1465
BsiEI CGRYCG 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 593
BslFI GGGAC 3 cut(s) 259, 563, 1061
BslI CCNNNNNNNGG 4 cut(s) 526, 887, 997, 1421
BsmAI GTCTC 2 cut(s) 61, 521
BsmFI GGGAC 3 cut(s) 259, 563, 1061
BsnI GGCC 6 cut(s) 186, 479, 504, 592, 853, 886
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 1237
Bsp1407I TGTACA 1 cut(s) 1354
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 4 cut(s) 187, 216, 881, 883
BspANI GGCC 6 cut(s) 186, 479, 504, 592, 853, 886
BspCNI CTCAG 1 cut(s) 1453
BspFNI CGCG 3 cut(s) 878, 883, 1340
BspLI GGNNCC 3 cut(s) 389, 1290, 1467
BspPI GGATC 2 cut(s) 1304, 1406
BspT104I TTCGAA 1 cut(s) 1237
BspT107I GGYRCC 2 cut(s) 1288, 1465
BsrDI GCAATG 2 cut(s) 1229, 1269
BsrFI RCCGGY 1 cut(s) 500
BsrGI TGTACA 1 cut(s) 1354
BsrI ACTGG 2 cut(s) 1089, 1426
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 6 cut(s) 121, 666, 881, 971, 1043, 1313
BssHII GCGCGC 1 cut(s) 876
BssNI GRCGYC 1 cut(s) 1466
BssT1I CCWWGG 2 cut(s) 121, 1313
Bst2UI CCWGG 2 cut(s) 888, 972
Bst4CI ACNGT 5 cut(s) 222, 492, 777, 809, 1000
BstACI GRCGYC 1 cut(s) 1466
BstAUI TGTACA 1 cut(s) 1354
BstBI TTCGAA 1 cut(s) 1237
BstC8I GCNNGC 4 cut(s) 802, 878, 893, 1034
BstDEI CTNAG 2 cut(s) 278, 1461
BstDSI CCRYGG 2 cut(s) 121, 881
BstF5I GGATG 4 cut(s) 118, 198, 793, 1111
BstFNI CGCG 3 cut(s) 878, 883, 1340
BstH2I RGCGCY 1 cut(s) 1469
BstHHI GCGC 3 cut(s) 878, 880, 1468
BstMAI GTCTC 2 cut(s) 61, 521
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 1 cut(s) 892
BstNI CCWGG 2 cut(s) 888, 972
BstNSI RCATGY 1 cut(s) 895
BstSCI CCNGG 6 cut(s) 358, 635, 886, 970, 990, 1042
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 3 cut(s) 878, 883, 1340
BstV1I GCAGC 1 cut(s) 1048
BstV2I GAAGAC 3 cut(s) 443, 1096, 1341
BstX2I RGATCY 2 cut(s) 565, 1309
BstYI RGATCY 2 cut(s) 565, 1309
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 6 cut(s) 186, 479, 504, 592, 853, 886
BtgI CCRYGG 2 cut(s) 121, 881
BtsCI GGATG 4 cut(s) 118, 198, 793, 1111
BtsIMutI CAGTG 2 cut(s) 814, 1419
Cac8I GCNNGC 4 cut(s) 802, 878, 893, 1034
CfoI GCGC 3 cut(s) 878, 880, 1468
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 341, 591, 663
Cfr42I CCGCGG 1 cut(s) 884
CseI GACGC 2 cut(s) 560, 1346
CviAII CATG 6 cut(s) 122, 149, 724, 892, 1216, 1453
DdeI CTNAG 2 cut(s) 278, 1461
DinI GGCGCC 1 cut(s) 1467
DraIII CACNNNGTG 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 184, 477
EagI CGGCCG 1 cut(s) 184
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 121, 1313
Eco147I AGGCCT 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 341, 663
Eco52I CGGCCG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 663
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 886, 970
EcoT14I CCWWGG 2 cut(s) 121, 1313
EgeI GGCGCC 1 cut(s) 1467
EheI GGCGCC 1 cut(s) 1467
ErhI CCWWGG 2 cut(s) 121, 1313
FaeI CATG 6 cut(s) 125, 152, 727, 895, 1219, 1456
FaqI GGGAC 3 cut(s) 259, 563, 1061
FatI CATG 6 cut(s) 121, 148, 723, 891, 1215, 1452
FauI CCCGC 1 cut(s) 223
FbaI TGATCA 2 cut(s) 283, 922
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 4 cut(s) 187, 881, 884, 1037
FokI GGATG 4 cut(s) 125, 185, 780, 1098
Fsp4HI GCNGC 4 cut(s) 187, 881, 884, 1037
FspBI CTAG 5 cut(s) 53, 300, 1059, 1314, 1416
GlaI GCGC 3 cut(s) 877, 879, 1467
GluI GCNGC 4 cut(s) 187, 881, 884, 1037
HaeII RGCGCY 1 cut(s) 1469
HaeIII GGCC 6 cut(s) 186, 479, 504, 592, 853, 886
HgaI GACGC 2 cut(s) 560, 1346
HhaI GCGC 3 cut(s) 878, 880, 1468
Hin1I GRCGYC 1 cut(s) 1466
Hin1II CATG 6 cut(s) 125, 152, 727, 895, 1219, 1456
Hin6I GCGC 3 cut(s) 876, 878, 1466
HinP1I GCGC 3 cut(s) 876, 878, 1466
HindIII AAGCTT 1 cut(s) 933
HinfI GANTC 5 cut(s) 173, 713, 1049, 1076, 1346
HphI GGTGA 5 cut(s) 137, 311, 385, 1198, 1405
Hpy166II GTNNAC 4 cut(s) 50, 118, 1128, 1303
Hpy188I TCNGA 2 cut(s) 670, 682
Hpy188III TCNNGA 4 cut(s) 658, 1015, 1320, 1367
Hpy8I GTNNAC 4 cut(s) 50, 118, 1128, 1303
HpyAV CCTTC 5 cut(s) 737, 1183, 1246, 1384, 1397
HpyCH4III ACNGT 5 cut(s) 222, 492, 777, 809, 1000
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 6 cut(s) 512, 895, 948, 958, 1036, 1430
HpyF10VI GCNNNNNNNGC 1 cut(s) 892
HpyF3I CTNAG 2 cut(s) 278, 1461
HpySE526I ACGT 1 cut(s) 440
Hsp92I GRCGYC 1 cut(s) 1466
Hsp92II CATG 6 cut(s) 125, 152, 727, 895, 1219, 1456
HspAI GCGC 3 cut(s) 876, 878, 1466
KasI GGCGCC 1 cut(s) 1465
KpnI GGTACC 1 cut(s) 1292
Ksp22I TGATCA 2 cut(s) 283, 922
KspI CCGCGG 1 cut(s) 884
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 1 cut(s) 1048
LweI GCATC 1 cut(s) 499
MaeI CTAG 5 cut(s) 53, 300, 1059, 1314, 1416
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 4 cut(s) 302, 631, 983, 994
MboII GAAGA 7 cut(s) 203, 448, 613, 1096, 1099, 1211, 1346
MflI RGATCY 2 cut(s) 565, 1309
MluI ACGCGT 1 cut(s) 1338
Mly113I GGCGCC 1 cut(s) 1466
MlyI GAGTC 1 cut(s) 1043
MmeI TCCRAC 1 cut(s) 734
MseI TTAA 1 cut(s) 978
MslI CAYNNNNRTG 1 cut(s) 728
MspA1I CMGCKG 2 cut(s) 883, 1352
MspR9I CCNGG 6 cut(s) 360, 637, 888, 972, 992, 1044
MvaI CCWGG 2 cut(s) 888, 972
MvnI CGCG 3 cut(s) 878, 883, 1340
MwoI GCNNNNNNNGC 1 cut(s) 892
NarI GGCGCC 1 cut(s) 1466
NciI CCSGG 4 cut(s) 360, 637, 992, 1044
NcoI CCATGG 1 cut(s) 121
NlaIII CATG 6 cut(s) 125, 152, 727, 895, 1219, 1456
NlaIV GGNNCC 3 cut(s) 389, 1290, 1467
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 895
NspV TTCGAA 1 cut(s) 1237
PaeI GCATGC 1 cut(s) 895
PalAI GGCGCGCC 1 cut(s) 876
PauI GCGCGC 1 cut(s) 876
PceI AGGCCT 1 cut(s) 853
PfeI GAWTC 4 cut(s) 173, 713, 1076, 1346
PkrI GCNGC 4 cut(s) 188, 882, 885, 1038
PleI GAGTC 1 cut(s) 1043
PluTI GGCGCC 1 cut(s) 1469
PpsI GAGTC 1 cut(s) 1043
PpuMI RGGWCCY 1 cut(s) 663
PshBI ATTAAT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 886, 970
PspGI CCWGG 2 cut(s) 886, 970
PspN4I GGNNCC 3 cut(s) 389, 1290, 1467
PspPI GGNCC 3 cut(s) 341, 591, 663
PspPPI RGGWCCY 1 cut(s) 663
PsrI GAACNNNNNNTAC 2 cut(s) 1347, 1379
PsuI RGATCY 2 cut(s) 565, 1309
PteI GCGCGC 1 cut(s) 876
PvuII CAGCTG 1 cut(s) 1352
RseI CAYNNNNRTG 1 cut(s) 728
SacII CCGCGG 1 cut(s) 884
SaqAI TTAA 1 cut(s) 978
SatI GCNGC 4 cut(s) 187, 881, 884, 1037
Sau96I GGNCC 3 cut(s) 341, 591, 663
ScaI AGTACT 1 cut(s) 1136
SchI GAGTC 1 cut(s) 1043
ScrFI CCNGG 6 cut(s) 360, 637, 888, 972, 992, 1044
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 1 cut(s) 28
SfoI GGCGCC 1 cut(s) 1467
Sfr303I CCGCGG 1 cut(s) 884
SfuI TTCGAA 1 cut(s) 1237
SgrBI CCGCGG 1 cut(s) 884
SgsI GGCGCGCC 1 cut(s) 876
SinI GGWCC 2 cut(s) 341, 663
SmiMI CAYNNNNRTG 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
SpeI ACTAGT 2 cut(s) 52, 299
SphI GCATGC 1 cut(s) 895
SseBI AGGCCT 1 cut(s) 853
SsiI CCGC 4 cut(s) 187, 216, 881, 883
SspDI GGCGCC 1 cut(s) 1465
SspI AATATT 1 cut(s) 108
SspMI CTAG 5 cut(s) 53, 300, 1059, 1314, 1416
StuI AGGCCT 1 cut(s) 853
StyD4I CCNGG 6 cut(s) 358, 635, 886, 970, 990, 1042
StyI CCWWGG 2 cut(s) 121, 1313
TaaI ACNGT 5 cut(s) 222, 492, 777, 809, 1000
TaiI ACGT 1 cut(s) 443
TaqI TCGA 8 cut(s) 260, 351, 507, 584, 938, 1014, 1237, 1319
TaqII GACCGA 1 cut(s) 1012
TatI WGTACW 4 cut(s) 49, 843, 1134, 1354
TauI GCSGC 3 cut(s) 189, 883, 886
TfiI GAWTC 4 cut(s) 173, 713, 1076, 1346
Tru1I TTAA 1 cut(s) 978
Tru9I TTAA 1 cut(s) 978
TscAI CASTG 2 cut(s) 814, 1426
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 1036
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 3 cut(s) 449, 782, 1441
TspRI CASTG 2 cut(s) 814, 1426
VpaK11BI GGWCC 2 cut(s) 341, 663
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 5 cut(s) 328, 898, 950, 1239, 1448
XceI RCATGY 1 cut(s) 895
XmaJI CCTAGG 1 cut(s) 1313
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 5 cut(s) 53, 300, 1059, 1314, 1416
ZrmI AGTACT 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.