MD04G1017700.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000894
Physical Location & Seq
Forward (+)
2070170 .. 2071648
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1017700.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGGAGAAACCACTCGCCGGAAAGTACTACAAAGAAAAATGGTTTGGTGTACTAGTCTCATTTTTCTTGTTCTTTGTATTGCTTTGCTTTGATTATTCAACTTCAATGTTGGGTGTCTACAGTGGGGTAAATTTGTTAGTCAATAAACATGTTCGTCCATTCTACCTCAAAGATTCGTGTTTTGGCCGCTACATTTACATCCACGATGATCTTCCAACACGGTTCAACTACGATCTCCTCGCAAACTGCCAGTCCCTTTCGACAGGAAGCAAGGCCCTTAGTGATCAAGGTGCGAATACTGGTGACAATATGTGTCCTCACCTTGTGAATTCGGGTCTTGGTCCTCAAATCGGAAAGCCCGGCAAGGTTTTGGCAAACAAGAGTTGGTTCATCACAAATCCCTACTTGTTAGAAGTCATTTTCCACAACAAGATGAAGACGTACAAATGCTTGACAAACAACTCCAATCTGGCTTCAGCCATTTATGTACCGTCATATACCGGCCTTGATGCGAGTCGCCATTTGTGGGATTTTGATCACAATGTCAGGGACGCGTCCGCGAAAGATCTTGTGAATTGGCTTACGCGGAGGCCTGAGTGGAAGAAAATGTGGGGGAGAGATCATTTTTTCGTTTCCGGGAGGACTTCTTGGGATTTCAGGAGGCCCGACAATGGTTCTGATTGGGGTACTAACTTAAGGTTTTTGCCGGGGTCGATGAACATGAGTATGTTGTCGGTTGAAGGAAGTTGTTGGAGAAATGACCTTGCTATACCGTACCCGACAAACTTCCATCCTTCAAACGATAGTGAGGTTGTTCAATGGCAGAACAGAGTGAAGAAGTACAAAAGGCCTCACTTGTTCTCTTTCATCGGTGCGCCGAGGCCTGGCATGCAAAAATCGATCAGGGGAAAACTTATCAATCAGTGCCAAGCATCAAACAACTGCAAGTTCGTGCATTGTGGTTCTCCTGGGATTAATTGTAACGACCCCGTTACAGTTATGAGGGTTTTCGAGAAGTCGGTCTATTGCTTGCAGCCTCCAGGTGATTCACACACAAGGAGATCAGCATTTGATTCTTTACTTGCTGGTTGTATTCCGGTCTTCTTTCATCCTGCGACTGCATACACGCAGTACTTATGGCATCTGCCGGAGAAACATACTAAGTATTCGGTGTTTATTCCGGTGAAGGAAGTGGAGGATTTGAAAGAAGGCATGATCGAGAAAACTTTGCTTCGAATTTCAGAAGAGAGGGAATTGGCAATGAGAGAGGAGGTCATAAGGCTGATACCAAAGCTGGTGTATGCAGATCCCAGGTCGAGATTAGAGAGCGAAGACGCGTTCGATTTAGCTGTGCAGGGAGTTCTTGAGAGGATAGAGAATGTTAGGAAGGTGATTAGAGAAGGGAAGGATCCTAGCGTTGGTTTTGCAGATGAGGATTGGACTAAATTCATGTTTCCGGAGGCACCAGAAGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

493

Amino Acids

56.56

Weight (kDa)

8.66

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 60 - 403 4.3e-76 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1462
AccI GTMKAC 1 cut(s) 117
AccII CGCG 4 cut(s) 554, 560, 586, 1337
AccIII TCCGGA 1 cut(s) 1456
AciI CCGC 3 cut(s) 187, 558, 586
AclWI GGATC 3 cut(s) 1301, 1403, 1416
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 4 cut(s) 130, 328, 1236, 1445
AcuI CTGAAG 1 cut(s) 459
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 8 cut(s) 26, 51, 443, 489, 688, 776, 842, 1133
AfiI CCNNNNNNNGG 6 cut(s) 17, 350, 526, 671, 884, 1418
AflII CTTAAG 1 cut(s) 694
AflIII ACRYGT 3 cut(s) 148, 552, 1335
AgsI TTSAA 7 cut(s) 99, 105, 226, 740, 798, 818, 1204
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 4 cut(s) 883, 967, 1039, 1310
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 2 cut(s) 1294, 1349
AluI AGCT 2 cut(s) 1294, 1349
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 3 cut(s) 1301, 1403, 1416
Aor13HI TCCGGA 1 cut(s) 1456
AoxI GGCC 7 cut(s) 184, 273, 502, 590, 662, 848, 881
ApeKI GCWGC 1 cut(s) 1033
ApoI RAATTY 4 cut(s) 130, 328, 1236, 1445
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AseI ATTAAT 1 cut(s) 975
AspLEI GCGC 1 cut(s) 877
AspS9I GGNCC 3 cut(s) 274, 341, 663
AsuC2I CCSGG 3 cut(s) 360, 637, 708
AsuHPI GGTGA 5 cut(s) 311, 314, 1055, 1195, 1402
AsuII TTCGAA 1 cut(s) 1234
AvaII GGWCC 1 cut(s) 341
BamHI GGATCC 1 cut(s) 1408
BanI GGYRCC 1 cut(s) 1462
BbsI GAAGAC 3 cut(s) 443, 1093, 1338
BbvI GCAGC 1 cut(s) 1045
BccI CCATC 1 cut(s) 798
BciT130I CCWGG 4 cut(s) 885, 969, 1041, 1312
BclI TGATCA 2 cut(s) 283, 535
BcnI CCSGG 3 cut(s) 360, 637, 708
BcoDI GTCTC 1 cut(s) 61
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 53, 1413
BfmI CTRYAG 1 cut(s) 118
BfrI CTTAAG 1 cut(s) 694
BglII AGATCT 1 cut(s) 565
BisI GCNGC 2 cut(s) 187, 1034
BlsI GCNGC 2 cut(s) 188, 1035
BmcAI AGTACT 2 cut(s) 26, 1133
Bme1390I CCNGG 7 cut(s) 360, 637, 708, 885, 969, 1041, 1312
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 3 cut(s) 274, 341, 663
BmiI GGNNCC 2 cut(s) 1410, 1464
BmrFI CCNGG 7 cut(s) 360, 637, 708, 885, 969, 1041, 1312
BmsI GCATC 3 cut(s) 499, 941, 1150
BpiI GAAGAC 3 cut(s) 443, 1093, 1338
BpmI CTGGAG 1 cut(s) 1023
Bpu14I TTCGAA 1 cut(s) 1234
BpuEI CTTGAG 1 cut(s) 1385
BpuMI CCSGG 3 cut(s) 360, 637, 708
Bsa29I ATCGAT 1 cut(s) 899
BsaBI GATNNNNATC 1 cut(s) 534
BsaJI CCNNGG 4 cut(s) 707, 878, 968, 1310
BsaWI WCCGGW 3 cut(s) 1096, 1180, 1456
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 350, 526, 671, 884, 1418
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 2 cut(s) 250, 304
Bse3DI GCAATG 1 cut(s) 1266
Bse8I GATNNNNATC 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 1456
BseBI CCWGG 4 cut(s) 885, 969, 1041, 1312
BseCI ATCGAT 1 cut(s) 899
BseDI CCNNGG 4 cut(s) 707, 878, 968, 1310
BseGI GGATG 3 cut(s) 198, 790, 1108
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 6 cut(s) 17, 350, 526, 671, 884, 1418
BseMI GCAATG 1 cut(s) 1266
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 2 cut(s) 250, 304
BseRI GAGGAG 2 cut(s) 227, 1283
BseXI GCAGC 1 cut(s) 1045
BsgI GTGCAG 1 cut(s) 1373
Bsh1236I CGCG 4 cut(s) 554, 560, 586, 1337
BshFI GGCC 7 cut(s) 186, 275, 504, 592, 664, 850, 883
BshNI GGYRCC 1 cut(s) 1462
BshVI ATCGAT 1 cut(s) 899
BsiSI CCGG 9 cut(s) 18, 360, 501, 636, 707, 1097, 1148, 1181, 1457
BslFI GGGAC 2 cut(s) 238, 563
BslI CCNNNNNNNGG 6 cut(s) 17, 350, 526, 671, 884, 1418
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 238, 563
BsnI GGCC 7 cut(s) 186, 275, 504, 592, 664, 850, 883
Bsp119I TTCGAA 1 cut(s) 1234
Bsp13I TCCGGA 1 cut(s) 1456
BspACI CCGC 3 cut(s) 187, 558, 586
BspANI GGCC 7 cut(s) 186, 275, 504, 592, 664, 850, 883
BspCNI CTCAG 1 cut(s) 586
BspDI ATCGAT 1 cut(s) 899
BspEI TCCGGA 1 cut(s) 1456
BspFNI CGCG 4 cut(s) 554, 560, 586, 1337
BspLI GGNNCC 2 cut(s) 1410, 1464
BspPI GGATC 3 cut(s) 1301, 1403, 1416
BspT104I TTCGAA 1 cut(s) 1234
BspT107I GGYRCC 1 cut(s) 1462
BspTI CTTAAG 1 cut(s) 694
BsrDI GCAATG 1 cut(s) 1266
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 2 cut(s) 250, 304
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 4 cut(s) 707, 878, 968, 1310
Bst2UI CCWGG 4 cut(s) 885, 969, 1041, 1312
Bst4CI ACNGT 5 cut(s) 122, 222, 492, 774, 997
Bst6I CTCTTC 1 cut(s) 1239
BstAFI CTTAAG 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 1234
BstC8I GCNNGC 2 cut(s) 890, 1031
BstDEI CTNAG 3 cut(s) 278, 594, 1161
BstF5I GGATG 3 cut(s) 198, 790, 1108
BstFNI CGCG 4 cut(s) 554, 560, 586, 1337
BstHHI GCGC 1 cut(s) 877
BstMAI GTCTC 1 cut(s) 61
BstMWI GCNNNNNNNGC 1 cut(s) 889
BstNI CCWGG 4 cut(s) 885, 969, 1041, 1312
BstNSI RCATGY 2 cut(s) 152, 892
BstSCI CCNGG 7 cut(s) 358, 635, 706, 883, 967, 1039, 1310
BstSFI CTRYAG 1 cut(s) 118
BstUI CGCG 4 cut(s) 554, 560, 586, 1337
BstV1I GCAGC 1 cut(s) 1045
BstV2I GAAGAC 3 cut(s) 443, 1093, 1338
BstX2I RGATCY 3 cut(s) 565, 1306, 1408
BstYI RGATCY 3 cut(s) 565, 1306, 1408
Bsu15I ATCGAT 1 cut(s) 899
BsuRI GGCC 7 cut(s) 186, 275, 504, 592, 664, 850, 883
BsuTUI ATCGAT 1 cut(s) 899
BtsCI GGATG 3 cut(s) 198, 790, 1108
BtsIMutI CAGTG 2 cut(s) 127, 929
Cac8I GCNNGC 2 cut(s) 890, 1031
CfoI GCGC 1 cut(s) 877
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 274, 341, 663
ClaI ATCGAT 1 cut(s) 899
CseI GACGC 3 cut(s) 543, 560, 1343
Csp6I GTAC 8 cut(s) 25, 50, 442, 488, 687, 775, 841, 1132
CviAII CATG 5 cut(s) 149, 721, 889, 1213, 1450
CviQI GTAC 8 cut(s) 25, 50, 442, 488, 687, 775, 841, 1132
DdeI CTNAG 3 cut(s) 278, 594, 1161
DraIII CACNNNGTG 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 1239
EarI CTCTTC 1 cut(s) 1239
Eco147I AGGCCT 3 cut(s) 592, 850, 883
Eco47I GGWCC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 4 cut(s) 883, 967, 1039, 1310
FaeI CATG 5 cut(s) 152, 724, 892, 1216, 1453
FaqI GGGAC 2 cut(s) 238, 563
FatI CATG 5 cut(s) 148, 720, 888, 1212, 1449
FbaI TGATCA 2 cut(s) 283, 535
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 187, 1034
FokI GGATG 3 cut(s) 185, 777, 1095
Fsp4HI GCNGC 2 cut(s) 187, 1034
FspBI CTAG 2 cut(s) 53, 1413
GlaI GCGC 1 cut(s) 876
GluI GCNGC 2 cut(s) 187, 1034
GsuI CTGGAG 1 cut(s) 1023
HaeIII GGCC 7 cut(s) 186, 275, 504, 592, 664, 850, 883
HapII CCGG 9 cut(s) 18, 360, 501, 636, 707, 1097, 1148, 1181, 1457
HgaI GACGC 3 cut(s) 543, 560, 1343
HhaI GCGC 1 cut(s) 877
Hin1II CATG 5 cut(s) 152, 724, 892, 1216, 1453
Hin6I GCGC 1 cut(s) 875
HinP1I GCGC 1 cut(s) 875
HinfI GANTC 4 cut(s) 173, 514, 1046, 1073
HpaII CCGG 9 cut(s) 18, 360, 501, 636, 707, 1097, 1148, 1181, 1457
HphI GGTGA 5 cut(s) 311, 314, 1055, 1195, 1402
Hpy166II GTNNAC 2 cut(s) 50, 118
Hpy188I TCNGA 3 cut(s) 353, 679, 1243
Hpy188III TCNNGA 6 cut(s) 658, 1012, 1219, 1317, 1364, 1457
Hpy8I GTNNAC 2 cut(s) 50, 118
HpyAV CCTTC 7 cut(s) 734, 804, 1180, 1202, 1381, 1394, 1399
HpyCH4III ACNGT 5 cut(s) 122, 222, 492, 774, 997
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 8 cut(s) 892, 945, 955, 1033, 1121, 1304, 1354, 1427
HpyF10VI GCNNNNNNNGC 1 cut(s) 889
HpyF3I CTNAG 3 cut(s) 278, 594, 1161
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 5 cut(s) 152, 724, 892, 1216, 1453
HspAI GCGC 1 cut(s) 875
Kpn2I TCCGGA 1 cut(s) 1456
Ksp22I TGATCA 2 cut(s) 283, 535
Lsp1109I GCAGC 1 cut(s) 1045
LweI GCATC 3 cut(s) 499, 941, 1150
MaeI CTAG 2 cut(s) 53, 1413
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 3 cut(s) 302, 980, 991
MboII GAAGA 7 cut(s) 203, 448, 613, 847, 1093, 1256, 1343
MflI RGATCY 3 cut(s) 565, 1306, 1408
MluCI AATT 7 cut(s) 130, 328, 574, 976, 1236, 1253, 1445
MluI ACGCGT 2 cut(s) 552, 1335
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 2 cut(s) 239, 731
MroI TCCGGA 1 cut(s) 1456
MseI TTAA 2 cut(s) 695, 975
MslI CAYNNNNRTG 1 cut(s) 725
MspCI CTTAAG 1 cut(s) 694
MspI CCGG 9 cut(s) 18, 360, 501, 636, 707, 1097, 1148, 1181, 1457
MspR9I CCNGG 7 cut(s) 360, 637, 708, 885, 969, 1041, 1312
MvaI CCWGG 4 cut(s) 885, 969, 1041, 1312
MvnI CGCG 4 cut(s) 554, 560, 586, 1337
MwoI GCNNNNNNNGC 1 cut(s) 889
NciI CCSGG 3 cut(s) 360, 637, 708
NlaIII CATG 5 cut(s) 152, 724, 892, 1216, 1453
NlaIV GGNNCC 2 cut(s) 1410, 1464
NmeAIII GCCGAG 1 cut(s) 903
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 2 cut(s) 152, 892
NspV TTCGAA 1 cut(s) 1234
PaeI GCATGC 1 cut(s) 892
PceI AGGCCT 3 cut(s) 592, 850, 883
PciI ACATGT 1 cut(s) 148
PfeI GAWTC 3 cut(s) 173, 1046, 1073
PfoI TCCNGGA 1 cut(s) 635
PkrI GCNGC 2 cut(s) 188, 1035
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
PscI ACATGT 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 975
Psp6I CCWGG 4 cut(s) 883, 967, 1039, 1310
PspGI CCWGG 4 cut(s) 883, 967, 1039, 1310
PspN4I GGNNCC 2 cut(s) 1410, 1464
PspPI GGNCC 3 cut(s) 274, 341, 663
PsuI RGATCY 3 cut(s) 565, 1306, 1408
RsaI GTAC 8 cut(s) 26, 51, 443, 489, 688, 776, 842, 1133
RsaNI GTAC 8 cut(s) 25, 50, 442, 488, 687, 775, 841, 1132
RseI CAYNNNNRTG 1 cut(s) 725
SaqAI TTAA 2 cut(s) 695, 975
SatI GCNGC 2 cut(s) 187, 1034
Sau96I GGNCC 3 cut(s) 274, 341, 663
ScaI AGTACT 2 cut(s) 26, 1133
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 7 cut(s) 360, 637, 708, 885, 969, 1041, 1312
SfaNI GCATC 3 cut(s) 499, 941, 1150
SfcI CTRYAG 1 cut(s) 118
SfuI TTCGAA 1 cut(s) 1234
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 2 cut(s) 694, 1364
SmoI CTYRAG 2 cut(s) 694, 1364
SpeI ACTAGT 1 cut(s) 52
SphI GCATGC 1 cut(s) 892
Sse9I AATT 7 cut(s) 130, 328, 574, 976, 1236, 1253, 1445
SseBI AGGCCT 3 cut(s) 592, 850, 883
SsiI CCGC 3 cut(s) 187, 558, 586
SspI AATATT 1 cut(s) 1475
SspMI CTAG 2 cut(s) 53, 1413
StuI AGGCCT 3 cut(s) 592, 850, 883
StyD4I CCNGG 7 cut(s) 358, 635, 706, 883, 967, 1039, 1310
TaaI ACNGT 5 cut(s) 122, 222, 492, 774, 997
TaiI ACGT 1 cut(s) 443
TaqI TCGA 8 cut(s) 260, 713, 899, 1011, 1218, 1234, 1316, 1341
TaqII GACCGA 1 cut(s) 1009
TasI AATT 7 cut(s) 130, 328, 574, 976, 1236, 1253, 1445
TatI WGTACW 4 cut(s) 24, 49, 840, 1131
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 3 cut(s) 173, 1046, 1073
Tru1I TTAA 2 cut(s) 695, 975
Tru9I TTAA 2 cut(s) 695, 975
TscAI CASTG 2 cut(s) 127, 929
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 1033
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 6 cut(s) 379, 449, 731, 856, 1097, 1438
TspRI CASTG 2 cut(s) 127, 929
Vha464I CTTAAG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 341
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 4 cut(s) 130, 328, 1236, 1445
XceI RCATGY 2 cut(s) 152, 892
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 2 cut(s) 53, 1413
ZrmI AGTACT 2 cut(s) 26, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.