MD00G1064500.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000894
Physical Location & Seq
Forward (+)
12160481 .. 12161731
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1064500.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGCACGACGATCTTCCTGAAATATTTAACTCCGACTTGGTAAAAAACTGTAAATTTCTCACCAGGGGAACTGACAAGCCAAATTTATGCATCTACTTTGAGAATTATGGTTTGGGTCCCGAAATCGAAAACTCGGAAGGGGTTTTGTCTAACAAGAGTTGGTTTTCCACGAACCAGTTCTTGCTGGAGTTCATATTCCACAACGAGATAAAGCACTATAAGTGTCTGACGAAAGTCTCCAGTCAGGCTTCGGCGGTTTATGTCCCGTTTTATGCCGGTCTTGATGCGAGCCTCCATCTGTGGGATTCTAATTTCACGGTTAGAGACGCTTCGGCGAAACAACTTGTTAGTTGGCTGGCAAGAAAACCCGAGTGGGCGAAAATGTGGGGGAGGGACCATTTCTTGGTTGCCGGGAGAATTTCTTGGGACTTCAGGAGGCAGACAGATGATGTTTCAGATTGGAGTAGAAGCTCTTGGAACAATGACATTGCTATACCTTATTCGACAAATTTTCATCCCGCGGAGGATGTTGATTTGGTTCAATGGCAGAGCAGAATGAGGAAACTAGAAAGGCCTTACTTGTTCACCTTTGCTGGGGCACCGAGGCCTGACCAGCAGAGTTCAATTCGAGGGAAGATTATTGATCTGTGCGTAGCTTCGAGTAGATGCAAGTTGATAGATTGCAGTTTGGGTGGTAACATAGATTGTGACAACCCGTCGAGTGTTACGAGGGTGTTTCAGAGCTCGGTTTATTGCTTGCAGCCTGGAGGGGATTCATACACCAGGAGATCAACTTTTGATTCTATTCTGGCAGGTTGTATTCCGGTTTTCTTTCACCCTGGTGCTGCTTATTCCCAATATTTGTGGAATTTGCCAAAGAATCATAGCAGCTACTCCGTGTATATTCCGGTGAGGGTTGCGGATGATATGCCAAGAAGCATTGAGGAAATCTTACTTGGAATTTCAAAGGAGAAAGAATTGGCCATTAGAGAGGAGGTTATAAGGCTAATACCAAATCTGATATATGTGGATCCGAGGTCTAGATTAGAGACTGAGGACGCATTCGATTTAGCCATCAAGGGAATTCTTGAGAGGATTGAGGATGTAAGGAAGGCGATTAGGGAAGGGAATGATCTGAGTATTGGTTTCGCAGATGAGGATAGCTACAAGTACACGTTTCATAAAAAACAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

399

Amino Acids

45.71

Weight (kDa)

5.8

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 1 - 313 7e-67 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
Acc36I ACCTGC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 598
AccB7I CCANNNNNTGG 1 cut(s) 403
AccII CGCG 1 cut(s) 521
AciI CCGC 4 cut(s) 254, 519, 521, 920
AclWI GGATC 2 cut(s) 1025, 1038
AcoI YGGCCR 1 cut(s) 981
AcsI RAATTY 7 cut(s) 53, 82, 417, 508, 868, 960, 1083
AcuI CTGAAG 1 cut(s) 415
AfaI GTAC 1 cut(s) 1172
AfiI CCNNNNNNNGG 3 cut(s) 301, 403, 594
AflIII ACRYGT 1 cut(s) 1173
AgsI TTSAA 3 cut(s) 542, 624, 966
AhdI GACNNNNNGTC 1 cut(s) 715
AjnI CCWGG 4 cut(s) 62, 763, 782, 838
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 127, 939, 971
AleI CACNNNNGTG 1 cut(s) 840
AluBI AGCT 5 cut(s) 471, 656, 744, 891, 1164
AluI AGCT 5 cut(s) 471, 656, 744, 891, 1164
Alw21I GWGCWC 1 cut(s) 746
Alw26I GTCTC 3 cut(s) 241, 318, 1043
AlwI GGATC 2 cut(s) 1025, 1038
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 3 cut(s) 572, 605, 981
ApeKI GCWGC 3 cut(s) 760, 845, 888
ApoI RAATTY 7 cut(s) 53, 82, 417, 508, 868, 960, 1083
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 116, 394
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 4 cut(s) 52, 577, 827, 922
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 116, 394
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 983
BamHI GGATCC 1 cut(s) 1030
BanI GGYRCC 1 cut(s) 598
BanII GRGCYC 1 cut(s) 746
Bbv12I GWGCWC 1 cut(s) 746
BbvI GCAGC 3 cut(s) 772, 832, 900
BccI CCATC 2 cut(s) 303, 1082
BciT130I CCWGG 4 cut(s) 64, 765, 784, 840
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 3 cut(s) 241, 318, 1043
BfaI CTAG 2 cut(s) 566, 1041
BfuAI ACCTGC 1 cut(s) 803
BisI GCNGC 3 cut(s) 761, 846, 889
BlsI GCNGC 3 cut(s) 762, 847, 890
Bme1390I CCNGG 5 cut(s) 64, 412, 765, 784, 840
Bme18I GGWCC 2 cut(s) 116, 394
BmeRI GACNNNNNGTC 1 cut(s) 715
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 116, 394
BmiI GGNNCC 5 cut(s) 117, 118, 395, 600, 1032
BmrFI CCNGG 5 cut(s) 64, 412, 765, 784, 840
BmsI GCATC 3 cut(s) 99, 274, 656
BoxI GACNNNNGTC 1 cut(s) 233
BpmI CTGGAG 3 cut(s) 206, 223, 786
BpuEI CTTGAG 1 cut(s) 1109
BpuMI CCSGG 1 cut(s) 412
BsaJI CCNNGG 5 cut(s) 63, 519, 602, 838, 1034
BsaWI WCCGGW 2 cut(s) 823, 907
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 403, 594
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 175, 240
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 4 cut(s) 64, 765, 784, 840
BseDI CCNNGG 5 cut(s) 63, 519, 602, 838, 1034
BseGI GGATG 4 cut(s) 514, 532, 928, 1108
BseLI CCNNNNNNNGG 3 cut(s) 301, 403, 594
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 2 cut(s) 1044, 1127
BseNI ACTGG 2 cut(s) 175, 240
BseRI GAGGAG 1 cut(s) 1007
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 3 cut(s) 772, 832, 900
BseYI CCCAGC 1 cut(s) 593
Bsh1236I CGCG 1 cut(s) 521
BshFI GGCC 3 cut(s) 574, 607, 983
BshNI GGYRCC 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 746
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 4 cut(s) 276, 411, 824, 908
BslFI GGGAC 4 cut(s) 102, 248, 407, 440
BslI CCNNNNNNNGG 3 cut(s) 301, 403, 594
BsmAI GTCTC 3 cut(s) 241, 318, 1043
BsmBI CGTCTC 1 cut(s) 318
BsmFI GGGAC 4 cut(s) 102, 248, 407, 440
BsmI GAATGC 1 cut(s) 1061
BsnI GGCC 3 cut(s) 574, 607, 983
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 601, 746
Bsp143I GATC 5 cut(s) 10, 643, 788, 1030, 1132
BspACI CCGC 4 cut(s) 254, 519, 521, 920
BspANI GGCC 3 cut(s) 574, 607, 983
BspCNI CTCAG 2 cut(s) 1045, 1128
BspFNI CGCG 1 cut(s) 521
BspLI GGNNCC 5 cut(s) 117, 118, 395, 600, 1032
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 2 cut(s) 1025, 1038
BspT107I GGYRCC 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 175, 240
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 5 cut(s) 63, 519, 602, 838, 1034
BssMI GATC 5 cut(s) 10, 643, 788, 1030, 1132
Bst2UI CCWGG 4 cut(s) 64, 765, 784, 840
Bst4CI ACNGT 2 cut(s) 50, 319
BstC8I GCNNGC 3 cut(s) 289, 357, 758
BstDEI CTNAG 2 cut(s) 1053, 1136
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 4 cut(s) 514, 532, 928, 1108
BstFNI CGCG 1 cut(s) 521
BstKTI GATC 5 cut(s) 13, 646, 791, 1033, 1135
BstMAI GTCTC 3 cut(s) 241, 318, 1043
BstMBI GATC 5 cut(s) 10, 643, 788, 1030, 1132
BstMWI GCNNNNNNNGC 1 cut(s) 613
BstNI CCWGG 4 cut(s) 64, 765, 784, 840
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 5 cut(s) 62, 410, 763, 782, 838
BstSLI GKGCMC 1 cut(s) 601
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 3 cut(s) 772, 832, 900
BstX2I RGATCY 1 cut(s) 1030
BstYI RGATCY 1 cut(s) 1030
BsuRI GGCC 3 cut(s) 574, 607, 983
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 4 cut(s) 514, 532, 928, 1108
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 3 cut(s) 289, 357, 758
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 116, 394
Cfr42I CCGCGG 1 cut(s) 522
CseI GACGC 2 cut(s) 335, 1067
Csp6I GTAC 1 cut(s) 1171
CspCI CAANNNNNGTGG 2 cut(s) 845, 880
CviQI GTAC 1 cut(s) 1171
DdeI CTNAG 2 cut(s) 1053, 1136
DpnI GATC 5 cut(s) 12, 645, 790, 1032, 1134
DpnII GATC 5 cut(s) 10, 643, 788, 1030, 1132
DriI GACNNNNNGTC 1 cut(s) 715
EaeI YGGCCR 1 cut(s) 981
Eam1105I GACNNNNNGTC 1 cut(s) 715
Ecl136II GAGCTC 1 cut(s) 744
Eco147I AGGCCT 2 cut(s) 574, 607
Eco24I GRGCYC 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 116, 394
Eco53kI GAGCTC 1 cut(s) 744
Eco57I CTGAAG 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 4 cut(s) 62, 763, 782, 838
EcoT22I ATGCAT 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 746
Esp3I CGTCTC 1 cut(s) 318
FaqI GGGAC 4 cut(s) 102, 248, 407, 440
FauI CCCGC 1 cut(s) 526
Fnu4HI GCNGC 3 cut(s) 761, 846, 889
FokI GGATG 4 cut(s) 501, 539, 935, 1115
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 3 cut(s) 761, 846, 889
FspBI CTAG 2 cut(s) 566, 1041
GluI GCNGC 3 cut(s) 761, 846, 889
GsaI CCCAGC 1 cut(s) 597
GsuI CTGGAG 3 cut(s) 206, 223, 786
HaeIII GGCC 3 cut(s) 574, 607, 983
HapII CCGG 4 cut(s) 276, 411, 824, 908
HgaI GACGC 2 cut(s) 335, 1067
HinfI GANTC 4 cut(s) 305, 773, 800, 880
HpaII CCGG 4 cut(s) 276, 411, 824, 908
HphI GGTGA 4 cut(s) 52, 577, 827, 922
Hpy166II GTNNAC 2 cut(s) 585, 1173
Hpy188I TCNGA 8 cut(s) 34, 136, 228, 457, 741, 1020, 1035, 1137
Hpy188III TCNNGA 6 cut(s) 17, 119, 281, 433, 1041, 1088
Hpy8I GTNNAC 2 cut(s) 585, 1173
Hpy99I CGWCG 2 cut(s) 11, 721
HpyAV CCTTC 3 cut(s) 131, 1105, 1118
HpyCH4III ACNGT 2 cut(s) 50, 319
HpyCH4IV ACGT 1 cut(s) 1175
HpyCH4V TGCA 5 cut(s) 4, 90, 669, 684, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 613
HpyF3I CTNAG 2 cut(s) 1053, 1136
HpySE526I ACGT 1 cut(s) 1175
KflI GGGWCCC 1 cut(s) 116
KspI CCGCGG 1 cut(s) 522
Kzo9I GATC 5 cut(s) 10, 643, 788, 1030, 1132
Lsp1109I GCAGC 3 cut(s) 772, 832, 900
LweI GCATC 3 cut(s) 99, 274, 656
MaeI CTAG 2 cut(s) 566, 1041
MaeII ACGT 1 cut(s) 1175
MaeIII GTNAC 3 cut(s) 695, 707, 724
MalI GATC 5 cut(s) 12, 645, 790, 1032, 1134
MboI GATC 5 cut(s) 10, 643, 788, 1030, 1132
MboII GAAGA 2 cut(s) 5, 646
MflI RGATCY 1 cut(s) 1030
MhlI GDGCHC 2 cut(s) 601, 746
MlsI TGGCCA 1 cut(s) 983
MluNI TGGCCA 1 cut(s) 983
MmeI TCCRAC 1 cut(s) 57
Mox20I TGGCCA 1 cut(s) 983
Mph1103I ATGCAT 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 983
MseI TTAA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 840
Msp20I TGGCCA 1 cut(s) 983
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 4 cut(s) 276, 411, 824, 908
MspR9I CCNGG 5 cut(s) 64, 412, 765, 784, 840
Mva1269I GAATGC 1 cut(s) 1061
MvaI CCWGG 4 cut(s) 64, 765, 784, 840
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 1 cut(s) 613
NciI CCSGG 1 cut(s) 412
NdeII GATC 5 cut(s) 10, 643, 788, 1030, 1132
NlaIV GGNNCC 5 cut(s) 117, 118, 395, 600, 1032
NmuCI GTSAC 1 cut(s) 707
NsiI ATGCAT 1 cut(s) 92
OliI CACNNNNGTG 1 cut(s) 840
PceI AGGCCT 2 cut(s) 574, 607
PcsI WCGNNNNNNNCGW 1 cut(s) 725
PctI GAATGC 1 cut(s) 1061
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 4 cut(s) 305, 773, 800, 880
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 3 cut(s) 762, 847, 890
PpuMI RGGWCCY 1 cut(s) 116
PshAI GACNNNNGTC 1 cut(s) 233
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 746
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 4 cut(s) 62, 763, 782, 838
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 4 cut(s) 62, 763, 782, 838
PspN4I GGNNCC 5 cut(s) 117, 118, 395, 600, 1032
PspPI GGNCC 2 cut(s) 116, 394
PspPPI RGGWCCY 1 cut(s) 116
PsuI RGATCY 1 cut(s) 1030
RsaI GTAC 1 cut(s) 1172
RsaNI GTAC 1 cut(s) 1171
RseI CAYNNNNRTG 1 cut(s) 840
SacI GAGCTC 1 cut(s) 746
SacII CCGCGG 1 cut(s) 522
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 3 cut(s) 761, 846, 889
Sau3AI GATC 5 cut(s) 10, 643, 788, 1030, 1132
Sau96I GGNCC 2 cut(s) 116, 394
ScrFI CCNGG 5 cut(s) 64, 412, 765, 784, 840
SduI GDGCHC 2 cut(s) 601, 746
SfaNI GCATC 3 cut(s) 99, 274, 656
Sfr303I CCGCGG 1 cut(s) 522
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 2 cut(s) 116, 394
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
SseBI AGGCCT 2 cut(s) 574, 607
SsiI CCGC 4 cut(s) 254, 519, 521, 920
SspI AATATT 2 cut(s) 24, 860
SspMI CTAG 2 cut(s) 566, 1041
SstI GAGCTC 1 cut(s) 746
StuI AGGCCT 2 cut(s) 574, 607
StyD4I CCNGG 5 cut(s) 62, 410, 763, 782, 838
TaaI ACNGT 2 cut(s) 50, 319
TaiI ACGT 1 cut(s) 1178
TaqI TCGA 6 cut(s) 126, 503, 628, 659, 719, 1065
TatI WGTACW 1 cut(s) 1170
TfiI GAWTC 4 cut(s) 305, 773, 800, 880
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 3 cut(s) 760, 845, 888
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 4 cut(s) 181, 503, 765, 1169
TspGWI ACGGA 1 cut(s) 886
Van91I CCANNNNNTGG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 116, 394
XapI RAATTY 7 cut(s) 53, 82, 417, 508, 868, 960, 1083
XbaI TCTAGA 1 cut(s) 1040
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 2 cut(s) 566, 1041
Zsp2I ATGCAT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.