RLG00000008220

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000894
Physical Location & Seq
Forward (+)
27337066 .. 27338636
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008220

Sequence Viewer

Length: 1485 bp
ATGGGGAAACCACTTGCTGGAAAGTGCTCTCCAGAAGTCTGGTTAGGCATTCTCATCTCTTTTGTTTTGTGCTTTTTTCTTCTGCTTCCCTTTGATTACCCAAGTAATTTTTTGGGGGTAAATAATGGTGTCAGTCTCTTTGAAAACAAAGAAACACACCCAATTGGTATTGTAAAGAATCAGTCGATTTCTGTCATCAACCAATCTACCACAGATTCATGCTCTGGTCGTTACATTTATATCCATGGTGATCTTCCTGCCAAATTCAACTTTGATCTGATCAACAACTGTGAGTCTCTTACTGGAAGTAACATGCCCAATATGTGTCCACACATAAAGAATTTCGGTTTTGGTCTGGAAATTAACAACTCTGAAGGGGTTTTAACCAACAAGAGATGGTTCTCCACTAACCAGTTCTCATTAGAACTCATATTTCACAGCAAGATGAAGCACTATGAGTGCTTGACAAACAACTCCGCTCAGGCTTCAGCTATTTATATACCAATCTATCCTGGCCTTGAGGAGAGTCAACATCTGTGGGATCCTAACCTCACAGCCAGAGACTCTTTCGGTGAAGAGTTTTCCAAGTGGGTTTCAAGTAGATTCGAATGGAAGAATATGTGGGGGAGAGACCATTTCTTTGTTTCAGGGAGGGTTGCTTTCGACTATAGGAGGAAAACAGACAACATTTCTGACTGGGGTAGTAAACTCAGGGTCTCGCCAGAGTCTAACAACATGACTATGTTGACAATTGAAGGAAGCTCACGGACAAATGATATTGCTGTACCATACCCCACTGGCTTTCATCCTGCAAAGGACAGTGAGGTGTTTCAGTGGCAGAGCAGAGTGAGGGGGCAAGAAAGGCCTTACTTGTTCACTTTTGTTGGTGGACCAAGGCCTGATCAGCCAAACTCTATCCGGGGCAAACTTTTTAATCAGTGTCAAGCCTCAAGTACTTGCAAGGTACTAAATTGCCATTCAAGTGGGATCAATTGTGAAGAAACGGTTACTGTTATGAGGGTGTTTCAGAAATCAGTTTATTGCTTGCACCCTACAGGGGATACATTCACTAGAAAATCAGCTTTCGACTCTATTCTGGCTGGTTGCATTCCTGTTTTCTTTCATCCGGCTAGTGCTTATAACCAGTATTTATGGTACTTGCCTAAGAATTACACCAAGTATTCTGTATTTATTCCAGTGAGGAAAGCACAAGATTTGAAAGACGGTAGCATTGAGAAAATCTTGCTTGGAATTTCAAAGGACATGGAATTCGCCATGAGAGAGCAAGTGATAAGCCTAATTCCAAAGTTGGTGTATGGAGATCCCAGGTCAAGATTAGAGAGCAAAGATGCATTTGATATTGCTGTCAACAAAATGCTCGAGAGGATAGAAAATGTAAGGAAGGTGATCAGAGAGGGGAGGGATCCTAGTACCGGATTTGCAGAGCCGGATGAATTCTTTTTGGCTCTTGAGGTTGGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

495

Amino Acids

56.17

Weight (kDa)

7.54

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 73 - 407 1.4e-74 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
AccB7I CCANNNNNTGG 1 cut(s) 17
AccBSI CCGCTC 1 cut(s) 479
AciI CCGC 1 cut(s) 477
AclWI GGATC 6 cut(s) 536, 549, 995, 1316, 1418, 1431
AcsI RAATTY 5 cut(s) 263, 340, 1251, 1268, 1454
AcuI CTGAAG 2 cut(s) 393, 471
AfaI GTAC 5 cut(s) 786, 955, 966, 1157, 1432
AfiI CCNNNNNNNGG 3 cut(s) 17, 1057, 1433
AgsI TTSAA 7 cut(s) 143, 268, 597, 755, 981, 1219, 1257
AjnI CCWGG 2 cut(s) 511, 1325
AluBI AGCT 3 cut(s) 491, 762, 1082
AluI AGCT 3 cut(s) 491, 762, 1082
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 5 cut(s) 140, 300, 555, 624, 721
AlwI GGATC 6 cut(s) 536, 549, 995, 1316, 1418, 1431
Ama87I CYCGRG 1 cut(s) 1379
AoxI GGCC 3 cut(s) 514, 863, 896
ApoI RAATTY 5 cut(s) 263, 340, 1251, 1268, 1454
ArsI GACNNNNNNTTYG 4 cut(s) 623, 655, 1253, 1285
AspS9I GGNCC 2 cut(s) 890, 1478
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 3 cut(s) 260, 584, 1417
AsuII TTCGAA 1 cut(s) 606
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 2 cut(s) 890, 1478
BamHI GGATCC 2 cut(s) 541, 1423
Bbv12I GWGCWC 1 cut(s) 29
BccI CCATC 1 cut(s) 390
BciT130I CCWGG 2 cut(s) 513, 1327
BciVI GTATCC 1 cut(s) 1054
BclI TGATCA 3 cut(s) 279, 901, 1407
BcnI CCSGG 1 cut(s) 920
BcoDI GTCTC 5 cut(s) 140, 300, 555, 624, 721
BfaI CTAG 3 cut(s) 1071, 1131, 1428
BfmI CTRYAG 2 cut(s) 667, 1053
BfuI GTATCC 1 cut(s) 1054
BmcAI AGTACT 1 cut(s) 955
Bme1390I CCNGG 3 cut(s) 513, 920, 1327
Bme18I GGWCC 2 cut(s) 890, 1478
BmeT110I CYCGRG 1 cut(s) 1379
BmgT120I GGNCC 2 cut(s) 890, 1478
BmiI GGNNCC 2 cut(s) 543, 1425
BmrFI CCNGG 3 cut(s) 513, 920, 1327
BmrI ACTGGG 1 cut(s) 706
BmsI GCATC 1 cut(s) 1339
BmuI ACTGGG 1 cut(s) 706
BpmI CTGGAG 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 606
BpuEI CTTGAG 2 cut(s) 539, 934
BpuMI CCSGG 1 cut(s) 920
BsaI GGTCTC 2 cut(s) 624, 721
BsaJI CCNNGG 4 cut(s) 244, 893, 919, 1325
BsaWI WCCGGW 1 cut(s) 1433
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 1057, 1433
Bse1I ACTGG 6 cut(s) 307, 412, 701, 802, 1144, 1196
BseBI CCWGG 2 cut(s) 513, 1327
BseDI CCNNGG 4 cut(s) 244, 893, 919, 1325
BseGI GGATG 3 cut(s) 805, 1123, 1456
BseLI CCNNNNNNNGG 3 cut(s) 17, 1057, 1433
BseMII CTCAG 2 cut(s) 494, 724
BseNI ACTGG 6 cut(s) 307, 412, 701, 802, 1144, 1196
BseRI GAGGAG 1 cut(s) 536
BshFI GGCC 3 cut(s) 516, 865, 898
BsiHKAI GWGCWC 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 1379
BsiSI CCGG 4 cut(s) 919, 1127, 1434, 1448
BslI CCNNNNNNNGG 3 cut(s) 17, 1057, 1433
BsmAI GTCTC 5 cut(s) 140, 300, 555, 624, 721
BsmI GAATGC 2 cut(s) 48, 1107
BsnI GGCC 3 cut(s) 516, 865, 898
Bso31I GGTCTC 2 cut(s) 624, 721
BsoBI CYCGRG 1 cut(s) 1379
Bsp119I TTCGAA 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 29
Bsp143I GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 477
BspANI GGCC 3 cut(s) 516, 865, 898
BspCNI CTCAG 2 cut(s) 493, 723
BspLI GGNNCC 2 cut(s) 543, 1425
BspPI GGATC 6 cut(s) 536, 549, 995, 1316, 1418, 1431
BspT104I TTCGAA 1 cut(s) 606
BspTNI GGTCTC 2 cut(s) 624, 721
BsrBI CCGCTC 1 cut(s) 479
BsrI ACTGG 6 cut(s) 307, 412, 701, 802, 1144, 1196
BssECI CCNNGG 4 cut(s) 244, 893, 919, 1325
BssMI GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
BssT1I CCWWGG 2 cut(s) 244, 893
Bst2UI CCWGG 2 cut(s) 513, 1327
Bst4CI ACNGT 5 cut(s) 290, 821, 1006, 1012, 1226
Bst6I CTCTTC 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 606
BstC8I GCNNGC 1 cut(s) 1046
BstDEI CTNAG 3 cut(s) 480, 710, 1164
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 3 cut(s) 805, 1123, 1456
BstKTI GATC 9 cut(s) 253, 277, 282, 544, 904, 990, 1324, 1410, 1426
BstMAI GTCTC 5 cut(s) 140, 300, 555, 624, 721
BstMBI GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
BstMWI GCNNNNNNNGC 2 cut(s) 862, 904
BstNI CCWGG 2 cut(s) 513, 1327
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 3 cut(s) 511, 918, 1325
BstSFI CTRYAG 2 cut(s) 667, 1053
BstX2I RGATCY 3 cut(s) 541, 1321, 1423
BstXI CCANNNNNNTGG 2 cut(s) 39, 983
BstYI RGATCY 3 cut(s) 541, 1321, 1423
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 3 cut(s) 516, 865, 898
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 805, 1123, 1456
BtsIMutI CAGTG 5 cut(s) 795, 826, 839, 944, 1203
Cac8I GCNNGC 1 cut(s) 1046
Cfr13I GGNCC 2 cut(s) 890, 1478
Csp6I GTAC 5 cut(s) 785, 954, 965, 1156, 1431
CspCI CAANNNNNGTGG 2 cut(s) 518, 553
CviAII CATG 7 cut(s) 219, 245, 313, 736, 1264, 1276, 1482
CviQI GTAC 5 cut(s) 785, 954, 965, 1156, 1431
DdeI CTNAG 3 cut(s) 480, 710, 1164
DpnI GATC 9 cut(s) 252, 276, 281, 543, 903, 989, 1323, 1409, 1425
DpnII GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 2 cut(s) 244, 893
Eco147I AGGCCT 2 cut(s) 865, 898
Eco31I GGTCTC 2 cut(s) 624, 721
Eco47I GGWCC 2 cut(s) 890, 1478
Eco57I CTGAAG 2 cut(s) 393, 471
Eco88I CYCGRG 1 cut(s) 1379
EcoRI GAATTC 2 cut(s) 1268, 1454
EcoRII CCWGG 2 cut(s) 511, 1325
EcoT14I CCWWGG 2 cut(s) 244, 893
EcoT22I ATGCAT 1 cut(s) 1354
ErhI CCWWGG 2 cut(s) 244, 893
FaeI CATG 7 cut(s) 222, 248, 316, 739, 1267, 1279, 1485
FatI CATG 7 cut(s) 218, 244, 312, 735, 1263, 1275, 1481
FbaI TGATCA 3 cut(s) 279, 901, 1407
FokI GGATG 3 cut(s) 792, 1110, 1463
FspBI CTAG 3 cut(s) 1071, 1131, 1428
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 3 cut(s) 516, 865, 898
HapII CCGG 4 cut(s) 919, 1127, 1434, 1448
Hin1II CATG 7 cut(s) 222, 248, 316, 739, 1267, 1279, 1485
HincII GTYRAC 3 cut(s) 530, 747, 1369
HindII GTYRAC 3 cut(s) 530, 747, 1369
HinfI GANTC 8 cut(s) 178, 215, 293, 526, 563, 603, 725, 1088
HpaII CCGG 4 cut(s) 919, 1127, 1434, 1448
HphI GGTGA 3 cut(s) 260, 584, 1417
Hpy166II GTNNAC 7 cut(s) 329, 530, 707, 747, 876, 890, 1369
Hpy188I TCNGA 5 cut(s) 279, 373, 694, 1029, 1412
Hpy188III TCNNGA 5 cut(s) 32, 356, 1332, 1381, 1469
Hpy8I GTNNAC 7 cut(s) 329, 530, 707, 747, 876, 890, 1369
HpyAV CCTTC 3 cut(s) 368, 749, 1396
HpyCH4III ACNGT 5 cut(s) 290, 821, 1006, 1012, 1226
HpyCH4V TGCA 6 cut(s) 812, 960, 1048, 1107, 1352, 1442
HpyF10VI GCNNNNNNNGC 2 cut(s) 862, 904
HpyF3I CTNAG 3 cut(s) 480, 710, 1164
Hsp92II CATG 7 cut(s) 222, 248, 316, 739, 1267, 1279, 1485
Ksp22I TGATCA 3 cut(s) 279, 901, 1407
Kzo9I GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
LweI GCATC 1 cut(s) 1339
MaeI CTAG 3 cut(s) 1071, 1131, 1428
MaeIII GTNAC 3 cut(s) 230, 308, 1006
MalI GATC 9 cut(s) 252, 276, 281, 543, 903, 989, 1323, 1409, 1425
MbiI CCGCTC 1 cut(s) 479
MboI GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
MboII GAAGA 5 cut(s) 71, 245, 587, 625, 1010
MfeI CAATTG 3 cut(s) 162, 750, 991
MflI RGATCY 3 cut(s) 541, 1321, 1423
MhlI GDGCHC 1 cut(s) 29
MlyI GAGTC 5 cut(s) 302, 535, 557, 734, 1082
Mph1103I ATGCAT 1 cut(s) 1354
MseI TTAA 3 cut(s) 363, 383, 933
MslI CAYNNNNRTG 2 cut(s) 740, 981
MspI CCGG 4 cut(s) 919, 1127, 1434, 1448
MspR9I CCNGG 3 cut(s) 513, 920, 1327
MunI CAATTG 3 cut(s) 162, 750, 991
Mva1269I GAATGC 2 cut(s) 48, 1107
MvaI CCWGG 2 cut(s) 513, 1327
MwoI GCNNNNNNNGC 2 cut(s) 862, 904
NciI CCSGG 1 cut(s) 920
NcoI CCATGG 1 cut(s) 244
NdeII GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
NlaIII CATG 7 cut(s) 222, 248, 316, 739, 1267, 1279, 1485
NlaIV GGNNCC 2 cut(s) 543, 1425
NsiI ATGCAT 1 cut(s) 1354
NspI RCATGY 1 cut(s) 316
NspV TTCGAA 1 cut(s) 606
PaeR7I CTCGAG 1 cut(s) 1379
PceI AGGCCT 2 cut(s) 865, 898
PctI GAATGC 2 cut(s) 48, 1107
PfeI GAWTC 3 cut(s) 178, 215, 603
PflMI CCANNNNNTGG 1 cut(s) 17
PleI GAGTC 5 cut(s) 301, 534, 557, 733, 1082
PpsI GAGTC 5 cut(s) 301, 534, 557, 733, 1082
PsiI TTATAA 1 cut(s) 1140
Psp6I CCWGG 2 cut(s) 511, 1325
PspGI CCWGG 2 cut(s) 511, 1325
PspN4I GGNNCC 2 cut(s) 543, 1425
PspPI GGNCC 2 cut(s) 890, 1478
PsuI RGATCY 3 cut(s) 541, 1321, 1423
RsaI GTAC 5 cut(s) 786, 955, 966, 1157, 1432
RsaNI GTAC 5 cut(s) 785, 954, 965, 1156, 1431
RseI CAYNNNNRTG 2 cut(s) 740, 981
SaqAI TTAA 3 cut(s) 363, 383, 933
Sau3AI GATC 9 cut(s) 250, 274, 279, 541, 901, 987, 1321, 1407, 1423
Sau96I GGNCC 2 cut(s) 890, 1478
ScaI AGTACT 1 cut(s) 955
SchI GAGTC 5 cut(s) 302, 535, 557, 734, 1082
ScrFI CCNGG 3 cut(s) 513, 920, 1327
SduI GDGCHC 1 cut(s) 29
SetI ASST 9 cut(s) 493, 552, 764, 828, 966, 1084, 1331, 1407, 1476
SfaNI GCATC 1 cut(s) 1339
SfcI CTRYAG 2 cut(s) 667, 1053
Sfr274I CTCGAG 1 cut(s) 1379
SfuI TTCGAA 1 cut(s) 606
SinI GGWCC 2 cut(s) 890, 1478
SlaI CTCGAG 1 cut(s) 1379
SmiMI CAYNNNNRTG 2 cut(s) 740, 981
SmlI CTYRAG 4 cut(s) 518, 949, 1379, 1469
SmoI CTYRAG 4 cut(s) 518, 949, 1379, 1469
SseBI AGGCCT 2 cut(s) 865, 898
SsiI CCGC 1 cut(s) 477
SspMI CTAG 3 cut(s) 1071, 1131, 1428
StuI AGGCCT 2 cut(s) 865, 898
StyD4I CCNGG 3 cut(s) 511, 918, 1325
StyI CCWWGG 2 cut(s) 244, 893
TaaI ACNGT 5 cut(s) 290, 821, 1006, 1012, 1226
TaqI TCGA 5 cut(s) 185, 606, 663, 1086, 1380
TatI WGTACW 1 cut(s) 953
TfiI GAWTC 3 cut(s) 178, 215, 603
Tru1I TTAA 3 cut(s) 363, 383, 933
Tru9I TTAA 3 cut(s) 363, 383, 933
TscAI CASTG 5 cut(s) 802, 826, 839, 944, 1203
TspDTI ATGAA 5 cut(s) 207, 461, 794, 1112, 1467
TspGWI ACGGA 1 cut(s) 781
TspRI CASTG 5 cut(s) 802, 826, 839, 944, 1203
Van91I CCANNNNNTGG 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 890, 1478
XapI RAATTY 5 cut(s) 263, 340, 1251, 1268, 1454
XceI RCATGY 1 cut(s) 316
XhoI CTCGAG 1 cut(s) 1379
XspI CTAG 3 cut(s) 1071, 1131, 1428
ZrmI AGTACT 1 cut(s) 955
Zsp2I ATGCAT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.