MD04G1017600.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000894
Physical Location & Seq
Forward (+)
2060062 .. 2061525
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1017600.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGGAGAAAGTACACACTGGAAAATGCCCCCAAGAAGTCTGGTTTGGCATCCTTATCTCCTTTGCTTTGTGTTTTGTCTTGTTTTCCTTTGATCATTCCACTTCTTTGAGTGTTGATGTTTTGGTGAATGAAAAAGAAAACCCAATTCGAGTTTTGAACTTTAGCCACACCAAAGATTCGTGTTCGGATCGATACATTTACATTCACGACAGTCTTCCCGCGAGATTCAACGACGATTTCATCAACAACTGTGAGTCTCTTAGTGCCGGAACTAGCATGACTAATGTCCCAAACATGTGTCCTCACCTTGTTAATTTGGGACTCGGTCCTAAAATTAACAACTCCGACGGTGTTTTATCGAACGCGGGCTGGTTCGTCACAAATCCCTACTTGTTGGAAGTCATATTCCACAACAGGATGAAGAAGTATGAGTGTCTGACAAGAAACTCAACTCTTGCTTCAGCCATTTATGTGCCGTTCTATCCTAGCATGGATGTTGGTGTCCATCTGTGGGATTCCAACATCGAAATCAGAGACTCTTCGGCTAAGGAGTTTGTCAAGTGGCTTTCGGGACAACCCGAGTGGACGAAGATGTGGGGGAGAGACCATTTCTTTGCTTCCGGGAGGATTTCTTGGGATTTCAGGAGGGAAAGAGACAATAGTTCGAATTGGGGTAGCAAACTCAGGTTCTTGCCCGAGTCTTTGAACATGACTATGTTGTCAATCGAAGGAAGCCGATGGAAAAATGACGTTGCAATTCCGTACCCAACAAACTTTCACCCTTCCAACGACAGCGAGGTGGTTCAATGGCAGGACAAGGTGAGAAAGGAAGAAAGGCCTTATTTATTTACTTTCGCCGGTGCGCCGAGGCCTAACCAGGAAGGTTCTATCCGGGGAAAACTTATCGAACACTGCCAAGCTTCGACTAATTGCAATTTCTTACATTGCACTGAAAAAATTTGTGGCAACCCGATTACTGTTATGAGGGCGTTTCAGAGCTCAGTTTATTGTTTGCAGCCTGCAGGGGATTCATTCACTCGGAGATCAACTTTTGATGCGTTTCTGGCTGGTTGTATCCCCGTTTTCTTTCATCCTGCTACCGCATACACTCAATATTTATGGTACTTGCCCAAGAATCATACCAAGTATTCGGTGTATATTCCGGTGAGGGATGTAACAGATTTGAAAGAAGGCAGCATTGAGAAAATCTTGCTCGGAATTTCAAAAGACAGGGAATTGGCAATGAGGGAGGAAGTTATAAGACTAATTCCGAAGCTGGTCTACGCAGATCCCAGCTCAAGATTAGAGACCAAAGACGCGTTTGATATAGCTGTGCAGGGAATTCTTGAGAGGATTGAGAATGTTAGGAAGGAGATCAGAGAGGGGAGAGATCCTAGTATTGGTTTTGCAGATGAGGATAATGGTAAGGTCACATTTCCAAAGACTATCGAAAGCCTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

488

Amino Acids

55.73

Weight (kDa)

6.37

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 61 - 396 2.7e-81 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1259
AasI GACNNNNNNGTC 1 cut(s) 718
AccI GTMKAC 1 cut(s) 1281
AccII CGCG 3 cut(s) 221, 365, 1319
AciI CCGC 3 cut(s) 219, 365, 1101
AclWI GGATC 3 cut(s) 195, 1283, 1385
AcsI RAATTY 3 cut(s) 957, 1218, 1341
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 3 cut(s) 12, 764, 1124
AfiI CCNNNNNNNGG 2 cut(s) 511, 1400
AflIII ACRYGT 2 cut(s) 294, 1317
AgsI TTSAA 6 cut(s) 157, 229, 706, 806, 1186, 1224
AjnI CCWGG 1 cut(s) 876
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 5 cut(s) 920, 999, 1276, 1296, 1331
AluI AGCT 5 cut(s) 920, 999, 1276, 1296, 1331
Alw21I GWGCWC 1 cut(s) 1001
Alw26I GTCTC 5 cut(s) 261, 528, 597, 648, 1301
AlwI GGATC 3 cut(s) 195, 1283, 1385
Ama87I CYCGRG 2 cut(s) 578, 695
AoxI GGCC 2 cut(s) 836, 869
ApeKI GCWGC 2 cut(s) 1015, 1194
ApoI RAATTY 3 cut(s) 957, 1218, 1341
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 2 cut(s) 622, 893
AsuHPI GGTGA 5 cut(s) 136, 296, 770, 832, 1177
AsuII TTCGAA 1 cut(s) 665
AvaI CYCGRG 2 cut(s) 578, 695
AvaII GGWCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 1001
BarI GAAGNNNNNNTAC 2 cut(s) 1265, 1297
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 1001
BbvI GCAGC 2 cut(s) 1027, 1206
BccI CCATC 2 cut(s) 513, 732
BceAI ACGGC 1 cut(s) 460
BciT130I CCWGG 1 cut(s) 878
BciVI GTATCC 1 cut(s) 1085
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 2 cut(s) 622, 893
BcoDI GTCTC 5 cut(s) 261, 528, 597, 648, 1301
BfaI CTAG 3 cut(s) 273, 486, 1395
BfmI CTRYAG 1 cut(s) 1020
BfuI GTATCC 1 cut(s) 1085
BisI GCNGC 2 cut(s) 1016, 1195
BlsI GCNGC 2 cut(s) 1017, 1196
Bme1390I CCNGG 3 cut(s) 622, 878, 893
Bme18I GGWCC 1 cut(s) 326
BmeT110I CYCGRG 2 cut(s) 578, 695
BmgT120I GGNCC 1 cut(s) 326
BmrFI CCNGG 3 cut(s) 622, 878, 893
BmsI GCATC 2 cut(s) 57, 1045
BoxI GACNNNNGTC 1 cut(s) 284
BpiI GAAGAC 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 665
BpuEI CTTGAG 2 cut(s) 1282, 1367
BpuMI CCSGG 2 cut(s) 622, 893
Bsa29I ATCGAT 1 cut(s) 190
BsaI GGTCTC 2 cut(s) 597, 1301
BsaJI CCNNGG 2 cut(s) 866, 892
BsaWI WCCGGW 1 cut(s) 1162
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 1400
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 2 cut(s) 943, 1248
BseBI CCWGG 1 cut(s) 878
BseCI ATCGAT 1 cut(s) 190
BseDI CCNNGG 2 cut(s) 866, 892
BseGI GGATG 5 cut(s) 48, 423, 499, 1090, 1177
BseLI CCNNNNNNNGG 2 cut(s) 511, 1400
BseMI GCAATG 2 cut(s) 943, 1248
BseMII CTCAG 2 cut(s) 697, 1014
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 1027, 1206
BseYI CCCAGC 1 cut(s) 1292
BsgI GTGCAG 1 cut(s) 1355
Bsh1236I CGCG 3 cut(s) 221, 365, 1319
BshFI GGCC 2 cut(s) 838, 871
BshVI ATCGAT 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 1001
BsiHKCI CYCGRG 2 cut(s) 578, 695
BsiSI CCGG 5 cut(s) 267, 621, 858, 892, 1163
BslFI GGGAC 3 cut(s) 272, 333, 585
BslI CCNNNNNNNGG 2 cut(s) 511, 1400
BsmAI GTCTC 5 cut(s) 261, 528, 597, 648, 1301
BsmFI GGGAC 3 cut(s) 272, 333, 585
BsnI GGCC 2 cut(s) 838, 871
Bso31I GGTCTC 2 cut(s) 597, 1301
BsoBI CYCGRG 2 cut(s) 578, 695
Bsp119I TTCGAA 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 1001
Bsp143I GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
BspACI CCGC 3 cut(s) 219, 365, 1101
BspANI GGCC 2 cut(s) 838, 871
BspCNI CTCAG 2 cut(s) 696, 1013
BspDI ATCGAT 1 cut(s) 190
BspFNI CGCG 3 cut(s) 221, 365, 1319
BspMAI CTGCAG 1 cut(s) 1024
BspPI GGATC 3 cut(s) 195, 1283, 1385
BspT104I TTCGAA 1 cut(s) 665
BspTNI GGTCTC 2 cut(s) 597, 1301
BsrDI GCAATG 2 cut(s) 943, 1248
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 2 cut(s) 866, 892
BssMI GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
Bst2UI CCWGG 1 cut(s) 878
Bst4CI ACNGT 4 cut(s) 212, 251, 350, 979
Bst6I CTCTTC 1 cut(s) 544
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 367, 1020
BstDEI CTNAG 4 cut(s) 260, 546, 683, 1000
BstF5I GGATG 5 cut(s) 48, 423, 499, 1090, 1177
BstFNI CGCG 3 cut(s) 221, 365, 1319
BstHHI GCGC 1 cut(s) 865
BstKTI GATC 6 cut(s) 94, 190, 1046, 1291, 1377, 1393
BstMAI GTCTC 5 cut(s) 261, 528, 597, 648, 1301
BstMBI GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 1064
BstNI CCWGG 1 cut(s) 878
BstNSI RCATGY 1 cut(s) 298
BstPAI GACNNNNGTC 1 cut(s) 284
BstSCI CCNGG 3 cut(s) 620, 876, 891
BstSFI CTRYAG 1 cut(s) 1020
BstUI CGCG 3 cut(s) 221, 365, 1319
BstV1I GCAGC 2 cut(s) 1027, 1206
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 2 cut(s) 1288, 1390
BstYI RGATCY 2 cut(s) 1288, 1390
Bsu15I ATCGAT 1 cut(s) 190
BsuI GTATCC 1 cut(s) 1085
BsuRI GGCC 2 cut(s) 838, 871
BsuTUI ATCGAT 1 cut(s) 190
BtsCI GGATG 5 cut(s) 48, 423, 499, 1090, 1177
BtsI GCAGTG 1 cut(s) 910
BtsIMutI CAGTG 3 cut(s) 15, 910, 948
Cac8I GCNNGC 2 cut(s) 367, 1020
CfoI GCGC 1 cut(s) 865
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 1 cut(s) 326
ClaI ATCGAT 1 cut(s) 190
CseI GACGC 1 cut(s) 1325
Csp6I GTAC 3 cut(s) 11, 763, 1123
CspCI CAANNNNNGTGG 2 cut(s) 563, 598
CviAII CATG 4 cut(s) 277, 295, 490, 709
CviQI GTAC 3 cut(s) 11, 763, 1123
DdeI CTNAG 4 cut(s) 260, 546, 683, 1000
DpnI GATC 6 cut(s) 93, 189, 1045, 1290, 1376, 1392
DpnII GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
DrdI GACNNNNNNGTC 1 cut(s) 718
DseDI GACNNNNNNGTC 1 cut(s) 718
Eam1104I CTCTTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 544
Ecl136II GAGCTC 1 cut(s) 999
Eco147I AGGCCT 2 cut(s) 838, 871
Eco24I GRGCYC 1 cut(s) 1001
Eco31I GGTCTC 2 cut(s) 597, 1301
Eco47I GGWCC 1 cut(s) 326
Eco53kI GAGCTC 1 cut(s) 999
Eco57I CTGAAG 1 cut(s) 444
Eco88I CYCGRG 2 cut(s) 578, 695
EcoICRI GAGCTC 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 1341
EcoRII CCWGG 1 cut(s) 876
EcoT38I GRGCYC 1 cut(s) 1001
FaeI CATG 4 cut(s) 280, 298, 493, 712
FaqI GGGAC 3 cut(s) 272, 333, 585
FatI CATG 4 cut(s) 276, 294, 489, 708
FauI CCCGC 2 cut(s) 226, 358
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 1281
Fnu4HI GCNGC 2 cut(s) 1016, 1195
FokI GGATG 5 cut(s) 35, 430, 506, 1077, 1184
FriOI GRGCYC 1 cut(s) 1001
Fsp4HI GCNGC 2 cut(s) 1016, 1195
FspBI CTAG 3 cut(s) 273, 486, 1395
GlaI GCGC 1 cut(s) 864
GluI GCNGC 2 cut(s) 1016, 1195
GsaI CCCAGC 1 cut(s) 1296
HaeIII GGCC 2 cut(s) 838, 871
HapII CCGG 5 cut(s) 267, 621, 858, 892, 1163
HgaI GACGC 1 cut(s) 1325
HhaI GCGC 1 cut(s) 865
Hin1II CATG 4 cut(s) 280, 298, 493, 712
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HindIII AAGCTT 1 cut(s) 918
HinfI GANTC 9 cut(s) 176, 225, 254, 321, 515, 536, 698, 1028, 1135
HpaII CCGG 5 cut(s) 267, 621, 858, 892, 1163
HphI GGTGA 5 cut(s) 136, 296, 770, 832, 1177
Hpy166II GTNNAC 3 cut(s) 13, 585, 1282
Hpy188I TCNGA 9 cut(s) 187, 346, 438, 533, 996, 1041, 1217, 1272, 1379
Hpy188III TCNNGA 5 cut(s) 206, 570, 643, 1299, 1346
Hpy8I GTNNAC 3 cut(s) 13, 585, 1282
Hpy99I CGWCG 2 cut(s) 236, 350
HpyAV CCTTC 5 cut(s) 722, 792, 875, 1184, 1363
HpyCH4III ACNGT 4 cut(s) 212, 251, 350, 979
HpyCH4IV ACGT 1 cut(s) 750
HpyCH4V TGCA 7 cut(s) 755, 933, 948, 1015, 1022, 1336, 1409
HpyF10VI GCNNNNNNNGC 1 cut(s) 1064
HpyF3I CTNAG 4 cut(s) 260, 546, 683, 1000
HpySE526I ACGT 1 cut(s) 750
Hsp92II CATG 4 cut(s) 280, 298, 493, 712
HspAI GCGC 1 cut(s) 863
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
Lsp1109I GCAGC 2 cut(s) 1027, 1206
LweI GCATC 2 cut(s) 57, 1045
MaeI CTAG 3 cut(s) 273, 486, 1395
MaeII ACGT 1 cut(s) 750
MaeIII GTNAC 3 cut(s) 376, 1174, 1429
MalI GATC 6 cut(s) 93, 189, 1045, 1290, 1376, 1392
MboI GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
MboII GAAGA 5 cut(s) 206, 433, 531, 601, 842
MflI RGATCY 2 cut(s) 1288, 1390
MhlI GDGCHC 1 cut(s) 1001
MluI ACGCGT 1 cut(s) 1317
MlyI GAGTC 4 cut(s) 263, 315, 530, 707
MmeI TCCRAC 4 cut(s) 369, 375, 543, 810
MseI TTAA 2 cut(s) 312, 336
MslI CAYNNNNRTG 2 cut(s) 470, 713
MspI CCGG 5 cut(s) 267, 621, 858, 892, 1163
MspR9I CCNGG 3 cut(s) 622, 878, 893
MvaI CCWGG 1 cut(s) 878
MvnI CGCG 3 cut(s) 221, 365, 1319
MwoI GCNNNNNNNGC 1 cut(s) 1064
NciI CCSGG 2 cut(s) 622, 893
NdeII GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
NlaIII CATG 4 cut(s) 280, 298, 493, 712
NmeAIII GCCGAG 1 cut(s) 891
NmuCI GTSAC 2 cut(s) 376, 1429
NspI RCATGY 1 cut(s) 298
NspV TTCGAA 1 cut(s) 665
PceI AGGCCT 2 cut(s) 838, 871
PciI ACATGT 1 cut(s) 294
PfeI GAWTC 5 cut(s) 176, 225, 515, 1028, 1135
PflFI GACNNNGTC 1 cut(s) 324
PfoI TCCNGGA 1 cut(s) 620
PkrI GCNGC 2 cut(s) 1017, 1196
PleI GAGTC 4 cut(s) 262, 315, 530, 706
PpsI GAGTC 4 cut(s) 262, 315, 530, 706
PscI ACATGT 1 cut(s) 294
PshAI GACNNNNGTC 1 cut(s) 284
PsiI TTATAA 1 cut(s) 1259
Psp124BI GAGCTC 1 cut(s) 1001
Psp6I CCWGG 1 cut(s) 876
PspFI CCCAGC 1 cut(s) 1292
PspGI CCWGG 1 cut(s) 876
PspPI GGNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 1024
PsuI RGATCY 2 cut(s) 1288, 1390
PsyI GACNNNGTC 1 cut(s) 324
RsaI GTAC 3 cut(s) 12, 764, 1124
RsaNI GTAC 3 cut(s) 11, 763, 1123
RseI CAYNNNNRTG 2 cut(s) 470, 713
SacI GAGCTC 1 cut(s) 1001
SaqAI TTAA 2 cut(s) 312, 336
SatI GCNGC 2 cut(s) 1016, 1195
Sau3AI GATC 6 cut(s) 91, 187, 1043, 1288, 1374, 1390
Sau96I GGNCC 1 cut(s) 326
SbfI CCTGCAGG 1 cut(s) 1024
SchI GAGTC 4 cut(s) 263, 315, 530, 707
ScrFI CCNGG 3 cut(s) 622, 878, 893
SdaI CCTGCAGG 1 cut(s) 1024
SduI GDGCHC 1 cut(s) 1001
SfaNI GCATC 2 cut(s) 57, 1045
SfcI CTRYAG 1 cut(s) 1020
SfuI TTCGAA 1 cut(s) 665
SgrAI CRCCGGYG 1 cut(s) 857
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 470, 713
SmlI CTYRAG 2 cut(s) 1297, 1346
SmoI CTYRAG 2 cut(s) 1297, 1346
Sse8387I CCTGCAGG 1 cut(s) 1024
SseBI AGGCCT 2 cut(s) 838, 871
SsiI CCGC 3 cut(s) 219, 365, 1101
SspI AATATT 1 cut(s) 1115
SspMI CTAG 3 cut(s) 273, 486, 1395
SstI GAGCTC 1 cut(s) 1001
StuI AGGCCT 2 cut(s) 838, 871
StyD4I CCNGG 3 cut(s) 620, 876, 891
TaaI ACNGT 4 cut(s) 212, 251, 350, 979
TaiI ACGT 1 cut(s) 753
TaqI TCGA 9 cut(s) 148, 190, 359, 525, 665, 726, 906, 923, 1449
TaqII GACCGA 1 cut(s) 314
TatI WGTACW 1 cut(s) 10
TfiI GAWTC 5 cut(s) 176, 225, 515, 1028, 1135
Tru1I TTAA 2 cut(s) 312, 336
Tru9I TTAA 2 cut(s) 312, 336
TscAI CASTG 3 cut(s) 22, 917, 955
TseFI GTSAC 2 cut(s) 376, 1429
TseI GCWGC 2 cut(s) 1015, 1194
Tsp45I GTSAC 2 cut(s) 376, 1429
TspDTI ATGAA 5 cut(s) 144, 229, 434, 1020, 1079
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 3 cut(s) 22, 917, 955
Tth111I GACNNNGTC 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 3 cut(s) 957, 1218, 1341
XceI RCATGY 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 1281
XspI CTAG 3 cut(s) 273, 486, 1395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.