pycom420g00900

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0000894
Physical Location & Seq
Forward (+)
1197205 .. 1198455
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00900.1

Sequence Viewer

Length: 1251 bp
ATGCACGACGATCTTCCTGAAATATTTAACTCCGACTTGGTAAAAAACTGTAAATTTCTCACCAGGGGAACTGACAAGCCAAATTTATGTACCTACTTTGAGAATTATGGTTTCGGTCCCGAAATCGAAAACTCGGAAGGGGTTTTGTCTAACAAGAGTTGGTTTTCCACGAACCAGTTCTTGCTGGAGTTCATATTCCACAACGAGATGAAGCACTACAAGTGTCTGACGAAAGTCTCCAGTCAGGCTTCGGCGGTTTATGTCCCGTTTTATGCCGGTCTTGATGCGACCCTCCATCTGTGGGCTTCTAATATCACGGTTAGAGACGCTTCGGCGAAACAACTTGTTAGTTGGCTGGCAAGAAAACCCGAGTGGGCGAAAATGTGGGGGAGGGACCATTTCTTGGTTGCCAGGAGAATTTCTTGGGACTTCAGGAGGCATACAGACGATGTTTCGGATTGGGGTAGTAAGCTCAGGTGGTTGCCTGAGTCTATGAACATGACTATGTTGTCGGTCGAAGGAAGCTCTTGGAACAATGACATTGCTATACCTTATCCGACAAATTTTCATCCCGCGGAGGATGTTGATTTGGTTCAATGGCAGAGCAGAATGAGGAAACTAGAAAGGCCTTACTTGTTCACTTTTGCTGGGGCACCGAGGCCTGACCAGCAGAGTTCAATTCAAGGGAAGATTATTGATCAGTGCGTAGCTTCGAGTAGATGCAAGTTGATAGATTGCAGTTTGGGTGGTAACATAGATTGTGACAACCCGTTGAGTGTTATGAGGGTGTTTCAGAGCTCAGTTTATTGCTTGCAGCCTGGAGGGGATTCATACACCAGGAGATCAACTTTTGATTCTATTCTGGCAGGTTGTATTCCGGTTTTCTTTCACCCTGGTGCTGCTTATTCCCAATATTTGTGGCATTTGCCAAAGAATCATAGCAGCTACTCCGTGTATATTCCGGTGAGGGTTGCAGATGATATGCCAAGAAGCATTGAGGAAATCTTGCTTGGAATTTCAAAGGAGAAAGAATTGGCCATTAGAGAGGAGGTTATAAGGCTAATACCGAATCTGGTATATGCGGATCCCAGGTCTAGATTAGAGACTGAGGACGCATTCGATTTAGCCATCAAGGGAATTCTTGACAGGATTAAGGATGTAAGGAAGGTGATTAGGGAAGGGAATGATCTGAGTATTGGTTTCACAGATGAGGATAGCTACAAGTACACGTTTCATAAAAAACAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

417

Amino Acids

47.87

Weight (kDa)

6.15

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 1 - 331 7.2e-75 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1055
AasI GACNNNNNNGTC 1 cut(s) 508
Acc36I ACCTGC 1 cut(s) 857
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 1 cut(s) 403
AccII CGCG 1 cut(s) 575
AciI CCGC 4 cut(s) 254, 573, 575, 1082
AclWI GGATC 2 cut(s) 1079, 1092
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 6 cut(s) 53, 82, 417, 562, 1014, 1137
AcuI CTGAAG 1 cut(s) 415
AfaI GTAC 2 cut(s) 91, 1226
AfiI CCNNNNNNNGG 2 cut(s) 301, 403
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 4 cut(s) 596, 678, 683, 1020
AjnI CCWGG 6 cut(s) 62, 410, 817, 836, 892, 1088
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
AleI CACNNNNGTG 1 cut(s) 894
AluBI AGCT 6 cut(s) 472, 525, 710, 798, 945, 1218
AluI AGCT 6 cut(s) 472, 525, 710, 798, 945, 1218
Alw21I GWGCWC 1 cut(s) 800
Alw26I GTCTC 3 cut(s) 241, 318, 1097
AlwI GGATC 2 cut(s) 1079, 1092
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 3 cut(s) 626, 659, 1035
ApeKI GCWGC 3 cut(s) 814, 899, 942
ApoI RAATTY 6 cut(s) 53, 82, 417, 562, 1014, 1137
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 116, 394
AsuHPI GGTGA 4 cut(s) 52, 881, 976, 1180
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 116, 394
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 1 cut(s) 1037
BamHI GGATCC 1 cut(s) 1084
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 1 cut(s) 800
Bbv12I GWGCWC 1 cut(s) 800
BbvI GCAGC 3 cut(s) 826, 886, 954
BccI CCATC 2 cut(s) 303, 1136
BciT130I CCWGG 6 cut(s) 64, 412, 819, 838, 894, 1090
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 3 cut(s) 241, 318, 1097
BfaI CTAG 2 cut(s) 620, 1095
BfuAI ACCTGC 1 cut(s) 857
BisI GCNGC 3 cut(s) 815, 900, 943
BlsI GCNGC 3 cut(s) 816, 901, 944
Bme1390I CCNGG 6 cut(s) 64, 412, 819, 838, 894, 1090
Bme18I GGWCC 2 cut(s) 116, 394
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 116, 394
BmiI GGNNCC 4 cut(s) 118, 395, 654, 1086
BmrFI CCNGG 6 cut(s) 64, 412, 819, 838, 894, 1090
BmsI GCATC 2 cut(s) 274, 710
BoxI GACNNNNGTC 1 cut(s) 233
BpmI CTGGAG 3 cut(s) 206, 223, 840
Bpu10I CCTNAGC 1 cut(s) 473
BsaJI CCNNGG 5 cut(s) 63, 573, 656, 892, 1088
BsaWI WCCGGW 2 cut(s) 877, 961
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 403
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 175, 240
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 6 cut(s) 64, 412, 819, 838, 894, 1090
BseDI CCNNGG 5 cut(s) 63, 573, 656, 892, 1088
BseGI GGATG 3 cut(s) 568, 586, 1162
BseLI CCNNNNNNNGG 2 cut(s) 301, 403
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 5 cut(s) 477, 487, 813, 1098, 1181
BseNI ACTGG 2 cut(s) 175, 240
BseRI GAGGAG 1 cut(s) 1061
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 3 cut(s) 826, 886, 954
BseYI CCCAGC 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 575
Bsh1285I CGRYCG 1 cut(s) 516
BshFI GGCC 3 cut(s) 628, 661, 1037
BshNI GGYRCC 1 cut(s) 652
BsiEI CGRYCG 1 cut(s) 516
BsiHKAI GWGCWC 1 cut(s) 800
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 276, 878, 962
BslFI GGGAC 4 cut(s) 102, 248, 407, 440
BslI CCNNNNNNNGG 2 cut(s) 301, 403
BsmAI GTCTC 3 cut(s) 241, 318, 1097
BsmBI CGTCTC 1 cut(s) 318
BsmFI GGGAC 4 cut(s) 102, 248, 407, 440
BsmI GAATGC 1 cut(s) 1115
BsnI GGCC 3 cut(s) 628, 661, 1037
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 655, 800
Bsp143I GATC 5 cut(s) 10, 697, 842, 1084, 1186
BspACI CCGC 4 cut(s) 254, 573, 575, 1082
BspANI GGCC 3 cut(s) 628, 661, 1037
BspCNI CTCAG 5 cut(s) 478, 486, 812, 1099, 1182
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 4 cut(s) 118, 395, 654, 1086
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 2 cut(s) 1079, 1092
BspT107I GGYRCC 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 175, 240
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 5 cut(s) 63, 573, 656, 892, 1088
BssMI GATC 5 cut(s) 10, 697, 842, 1084, 1186
Bst2UI CCWGG 6 cut(s) 64, 412, 819, 838, 894, 1090
Bst4CI ACNGT 2 cut(s) 50, 319
BstC8I GCNNGC 2 cut(s) 357, 812
BstDEI CTNAG 5 cut(s) 473, 486, 799, 1107, 1190
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 3 cut(s) 568, 586, 1162
BstFNI CGCG 1 cut(s) 575
BstKTI GATC 5 cut(s) 13, 700, 845, 1087, 1189
BstMAI GTCTC 3 cut(s) 241, 318, 1097
BstMBI GATC 5 cut(s) 10, 697, 842, 1084, 1186
BstMCI CGRYCG 1 cut(s) 516
BstMWI GCNNNNNNNGC 1 cut(s) 667
BstNI CCWGG 6 cut(s) 64, 412, 819, 838, 894, 1090
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 6 cut(s) 62, 410, 817, 836, 892, 1088
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 3 cut(s) 826, 886, 954
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BsuRI GGCC 3 cut(s) 628, 661, 1037
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 3 cut(s) 568, 586, 1162
BtsIMutI CAGTG 1 cut(s) 707
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 2 cut(s) 357, 812
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 116, 394
Cfr42I CCGCGG 1 cut(s) 576
CseI GACGC 2 cut(s) 335, 1121
Csp6I GTAC 2 cut(s) 90, 1225
CspCI CAANNNNNGTGG 2 cut(s) 899, 934
CviAII CATG 1 cut(s) 499
CviQI GTAC 2 cut(s) 90, 1225
DdeI CTNAG 5 cut(s) 473, 486, 799, 1107, 1190
DpnI GATC 5 cut(s) 12, 699, 844, 1086, 1188
DpnII GATC 5 cut(s) 10, 697, 842, 1084, 1186
DrdI GACNNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 1035
Ecl136II GAGCTC 1 cut(s) 798
Eco147I AGGCCT 2 cut(s) 628, 661
Eco24I GRGCYC 1 cut(s) 800
Eco47I GGWCC 2 cut(s) 116, 394
Eco53kI GAGCTC 1 cut(s) 798
Eco57I CTGAAG 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 798
EcoRI GAATTC 1 cut(s) 1137
EcoRII CCWGG 6 cut(s) 62, 410, 817, 836, 892, 1088
EcoT38I GRGCYC 1 cut(s) 800
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 1 cut(s) 502
FaqI GGGAC 4 cut(s) 102, 248, 407, 440
FatI CATG 1 cut(s) 498
FauI CCCGC 1 cut(s) 580
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 3 cut(s) 815, 900, 943
FokI GGATG 3 cut(s) 555, 593, 1169
FriOI GRGCYC 1 cut(s) 800
Fsp4HI GCNGC 3 cut(s) 815, 900, 943
FspBI CTAG 2 cut(s) 620, 1095
GluI GCNGC 3 cut(s) 815, 900, 943
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 3 cut(s) 206, 223, 840
HaeIII GGCC 3 cut(s) 628, 661, 1037
HapII CCGG 3 cut(s) 276, 878, 962
HgaI GACGC 2 cut(s) 335, 1121
Hin1II CATG 1 cut(s) 502
HinfI GANTC 5 cut(s) 488, 827, 854, 934, 1069
HpaII CCGG 3 cut(s) 276, 878, 962
HphI GGTGA 4 cut(s) 52, 881, 976, 1180
Hpy166II GTNNAC 2 cut(s) 639, 1227
Hpy188I TCNGA 7 cut(s) 34, 136, 228, 457, 558, 795, 1191
Hpy188III TCNNGA 6 cut(s) 17, 119, 281, 433, 1095, 1142
Hpy8I GTNNAC 2 cut(s) 639, 1227
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 131, 512, 1159, 1172
HpyCH4III ACNGT 2 cut(s) 50, 319
HpyCH4IV ACGT 1 cut(s) 1229
HpyCH4V TGCA 5 cut(s) 4, 723, 738, 814, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 667
HpyF3I CTNAG 5 cut(s) 473, 486, 799, 1107, 1190
HpySE526I ACGT 1 cut(s) 1229
Hsp92II CATG 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 697
KspI CCGCGG 1 cut(s) 576
Kzo9I GATC 5 cut(s) 10, 697, 842, 1084, 1186
Lsp1109I GCAGC 3 cut(s) 826, 886, 954
LweI GCATC 2 cut(s) 274, 710
MaeI CTAG 2 cut(s) 620, 1095
MaeII ACGT 1 cut(s) 1229
MaeIII GTNAC 2 cut(s) 749, 761
MalI GATC 5 cut(s) 12, 699, 844, 1086, 1188
MboI GATC 5 cut(s) 10, 697, 842, 1084, 1186
MboII GAAGA 2 cut(s) 5, 700
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 2 cut(s) 655, 800
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 9 cut(s) 53, 82, 103, 417, 562, 678, 1014, 1031, 1137
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 2 cut(s) 57, 581
Mox20I TGGCCA 1 cut(s) 1037
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 1037
MseI TTAA 2 cut(s) 27, 1152
MslI CAYNNNNRTG 2 cut(s) 503, 894
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 3 cut(s) 276, 878, 962
MspR9I CCNGG 6 cut(s) 64, 412, 819, 838, 894, 1090
Mva1269I GAATGC 1 cut(s) 1115
MvaI CCWGG 6 cut(s) 64, 412, 819, 838, 894, 1090
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 1 cut(s) 667
NdeII GATC 5 cut(s) 10, 697, 842, 1084, 1186
NlaIII CATG 1 cut(s) 502
NlaIV GGNNCC 4 cut(s) 118, 395, 654, 1086
NmuCI GTSAC 1 cut(s) 761
OliI CACNNNNGTG 1 cut(s) 894
PceI AGGCCT 2 cut(s) 628, 661
PctI GAATGC 1 cut(s) 1115
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 4 cut(s) 827, 854, 934, 1069
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 3 cut(s) 816, 901, 944
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 233
PsiI TTATAA 1 cut(s) 1055
Psp124BI GAGCTC 1 cut(s) 800
Psp6I CCWGG 6 cut(s) 62, 410, 817, 836, 892, 1088
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 6 cut(s) 62, 410, 817, 836, 892, 1088
PspN4I GGNNCC 4 cut(s) 118, 395, 654, 1086
PspPI GGNCC 2 cut(s) 116, 394
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 2 cut(s) 91, 1226
RsaNI GTAC 2 cut(s) 90, 1225
RseI CAYNNNNRTG 2 cut(s) 503, 894
SacI GAGCTC 1 cut(s) 800
SacII CCGCGG 1 cut(s) 576
SaqAI TTAA 2 cut(s) 27, 1152
SatI GCNGC 3 cut(s) 815, 900, 943
Sau3AI GATC 5 cut(s) 10, 697, 842, 1084, 1186
Sau96I GGNCC 2 cut(s) 116, 394
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 6 cut(s) 64, 412, 819, 838, 894, 1090
SduI GDGCHC 2 cut(s) 655, 800
SfaNI GCATC 2 cut(s) 274, 710
Sfr303I CCGCGG 1 cut(s) 576
SgrBI CCGCGG 1 cut(s) 576
SinI GGWCC 2 cut(s) 116, 394
SmiMI CAYNNNNRTG 2 cut(s) 503, 894
Sse9I AATT 9 cut(s) 53, 82, 103, 417, 562, 678, 1014, 1031, 1137
SseBI AGGCCT 2 cut(s) 628, 661
SsiI CCGC 4 cut(s) 254, 573, 575, 1082
SspI AATATT 2 cut(s) 24, 914
SspMI CTAG 2 cut(s) 620, 1095
SstI GAGCTC 1 cut(s) 800
StuI AGGCCT 2 cut(s) 628, 661
StyD4I CCNGG 6 cut(s) 62, 410, 817, 836, 892, 1088
TaaI ACNGT 2 cut(s) 50, 319
TaiI ACGT 1 cut(s) 1232
TaqI TCGA 4 cut(s) 126, 516, 713, 1119
TaqII GACCGA 2 cut(s) 104, 502
TasI AATT 9 cut(s) 53, 82, 103, 417, 562, 678, 1014, 1031, 1137
TatI WGTACW 1 cut(s) 1224
TfiI GAWTC 4 cut(s) 827, 854, 934, 1069
Tru1I TTAA 2 cut(s) 27, 1152
Tru9I TTAA 2 cut(s) 27, 1152
TscAI CASTG 1 cut(s) 707
TseFI GTSAC 1 cut(s) 761
TseI GCWGC 3 cut(s) 814, 899, 942
Tsp45I GTSAC 1 cut(s) 761
TspDTI ATGAA 6 cut(s) 181, 224, 509, 557, 819, 1223
TspGWI ACGGA 1 cut(s) 940
TspRI CASTG 1 cut(s) 707
Van91I CCANNNNNTGG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 116, 394
XapI RAATTY 6 cut(s) 53, 82, 417, 562, 1014, 1137
XbaI TCTAGA 1 cut(s) 1094
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 2 cut(s) 620, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.