MD04G1017500.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000894
Physical Location & Seq
Forward (+)
2052918 .. 2054375
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1017500.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGCCAGAAAGTTATCCCAAGAAGTCTGGGTTGGCATACTAGTCACTTTTTATATTTTATGCATTTCTTTGTTTTGTTTAAGGTATTCAACTTCATTGGGAGCCAACAATGTGATCAATCTTTTGGTGAATAAACAAGTTCACCCGACTTGTACGATGAAGATCAATCACACCAACAGCGATTCATGCTTGAATCGTTACATTTACATCCATGACGGTCTTCCTGCAAGGTTCAACTATGACTTCCTCAACAATTGTGAGTCTCTTAGCCCAAGAGCTAGTAGTAGCAACAAACCGAGTATGTGTCCACAGCTTGTAAATTTGGGGCTTGGTCCGGATGTTAGCAAATCGGAAGGAGTTTTGTCCGACAAAAGTTGGTATCAAACAAATCCCCACTTGTTAGAAGTCATATTTCACAACAGGATGAAGAAGCACGAGTGTTTGACAAGCAACTCCAATCTTGCTTCAGCCATTTATGTGCCATTCTATCCTAGCATGGGCGTTGGTGTCCATCTGTGGGATTCTAACGTCGAAATCAGAGACGCTTCAGCCAAGGATTTTGTCAAATGGATTTCGAGACAACCCGAATGGAGGAAAATGTGGGGGAGAGACCATTTCTTTGCTTATGGGAGGACTGCTTGGGATTTCAGAAGGCAAGAGGATGATAGTTCTGATTGGGGAAGTAAACTCAGGTTCTTGCCCGAGTCCCTGAACATGACTATATTAATAGAAGGAAGCGAATGGAAAAACGACATCGCAATTCCGTACCCGACAAACTTTCATCCTACAGAGGACAGTGAGGTGGTTGCATGGCAAAACAAGGTGAGGAAACAGGAAAGGCCTTATTTGTTTACTTTCGCAGGTGCACCGAGGCCTAACTTGGAAGGTTCTATCCAGGGAAAACTTATCGAACATTGCCAAGCTTCAAATAACTGCAATTTTTTACACTGCACTGAAAAGATTTGTGGCAACCCGATTACAGTTATGAGGGCGTTTCAGAGCTCAGTTTACTGCTTGCAGCCAGTCGGGGACTCATTCACTAGGAGATCAGCTTTCGACGCGTTTCTGGCTGGTTGCATTCCAGTTTTCTTTCATCCAGCTACCGCGTACGCTCAATATTTATGGTACTTACCAAAGGATCATACCAAGTATTCGGTGTTTATTCCCGCGAAGGATGGAAATGATTTGAAAGAAGGCAGAATTGAGAATATCTTGCTTGGAATTTCGAAGGAAAAGGGGGCGGCCATGCGAGAGGAGGTCATAAGGCTAATACCAAAGCTGGTATATGCAGATCCCAGGGGAAGATTAAAGACTCCCGATGCATTTGATATAGCTGTGCAAGGAATGCTTGACAGAATAGAGAATGTTAGAAAGGTGATGAATGAGGGAAGGGATCCAAGTGCTGACTTTGCAGGTCTTGAGAATGATAAGTTCAAATTTCCTGACAGTACAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

486

Amino Acids

55.33

Weight (kDa)

7.89

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 64 - 397 1.6e-75 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
Acc36I ACCTGC 2 cut(s) 851, 1403
AccII CGCG 3 cut(s) 1061, 1106, 1169
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 3 cut(s) 1104, 1167, 1241
AclWI GGATC 4 cut(s) 1146, 1286, 1388, 1401
AcoI YGGCCR 2 cut(s) 3, 1242
AcsI RAATTY 3 cut(s) 319, 1221, 1436
AcuI CTGAAG 2 cut(s) 450, 531
AfaI GTAC 5 cut(s) 154, 767, 1109, 1127, 1450
AfiI CCNNNNNNNGG 3 cut(s) 497, 517, 591
AflIII ACRYGT 1 cut(s) 1059
AgsI TTSAA 6 cut(s) 90, 193, 235, 927, 1189, 1435
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 2 cut(s) 894, 1295
AjuI GAANNNNNNNTTGG 2 cut(s) 15, 47
AluBI AGCT 8 cut(s) 278, 313, 923, 1002, 1052, 1100, 1279, 1334
AluI AGCT 8 cut(s) 278, 313, 923, 1002, 1052, 1100, 1279, 1334
Alw21I GWGCWC 2 cut(s) 868, 1004
Alw26I GTCTC 4 cut(s) 267, 534, 571, 603
Alw44I GTGCAC 1 cut(s) 864
AlwI GGATC 4 cut(s) 1146, 1286, 1388, 1401
Ama87I CYCGRG 1 cut(s) 701
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 4 cut(s) 3, 839, 872, 1242
ApaLI GTGCAC 1 cut(s) 864
ApeKI GCWGC 1 cut(s) 1018
ApoI RAATTY 3 cut(s) 319, 1221, 1436
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AseI ATTAAT 1 cut(s) 725
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 4 cut(s) 134, 139, 835, 1387
AsuII TTCGAA 1 cut(s) 1226
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 868
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1393
BanII GRGCYC 1 cut(s) 1004
BauI CACGAG 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 868, 1004
BbvI GCAGC 1 cut(s) 1030
BccI CCATC 2 cut(s) 519, 1169
BciT130I CCWGG 2 cut(s) 896, 1297
BclI TGATCA 1 cut(s) 114
BcoDI GTCTC 4 cut(s) 267, 534, 571, 603
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 279, 492, 1041
BfmI CTRYAG 1 cut(s) 786
BfuAI ACCTGC 2 cut(s) 851, 1403
BisI GCNGC 2 cut(s) 1019, 1242
BlsI GCNGC 2 cut(s) 1020, 1243
Bme1390I CCNGG 2 cut(s) 896, 1297
Bme18I GGWCC 1 cut(s) 332
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 103, 1395
BmrFI CCNGG 2 cut(s) 896, 1297
BmsI GCATC 1 cut(s) 1309
BpiI GAAGAC 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 1226
BpuEI CTTGAG 1 cut(s) 1439
BsaBI GATNNNNATC 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 5 cut(s) 552, 869, 895, 1295, 1296
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 517, 591
Bse1I ACTGG 2 cut(s) 1022, 1082
Bse3DI GCAATG 1 cut(s) 913
Bse8I GATNNNNATC 1 cut(s) 161
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 2 cut(s) 896, 1297
BseDI CCNNGG 5 cut(s) 552, 869, 895, 1295, 1296
BseGI GGATG 7 cut(s) 207, 343, 429, 667, 781, 1093, 1180
BseJI GATNNNNATC 1 cut(s) 161
BseLI CCNNNNNNNGG 3 cut(s) 497, 517, 591
BseMI GCAATG 1 cut(s) 913
BseMII CTCAG 2 cut(s) 703, 1017
BseNI ACTGG 2 cut(s) 1022, 1082
BseRI GAGGAG 1 cut(s) 1268
BseSI GKGCMC 1 cut(s) 868
BseXI GCAGC 1 cut(s) 1030
BsgI GTGCAG 1 cut(s) 934
Bsh1236I CGCG 3 cut(s) 1061, 1106, 1169
BshFI GGCC 4 cut(s) 5, 841, 874, 1244
BsiHKAI GWGCWC 2 cut(s) 868, 1004
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 1 cut(s) 335
BsiWI CGTACG 1 cut(s) 1107
BslFI GGGAC 2 cut(s) 691, 1043
BslI CCNNNNNNNGG 3 cut(s) 497, 517, 591
BsmAI GTCTC 4 cut(s) 267, 534, 571, 603
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 2 cut(s) 691, 1043
BsmI GAATGC 2 cut(s) 1077, 1350
BsnI GGCC 4 cut(s) 5, 841, 874, 1244
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 701
Bsp119I TTCGAA 1 cut(s) 1226
Bsp1286I GDGCHC 2 cut(s) 868, 1004
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
BspACI CCGC 3 cut(s) 1104, 1167, 1241
BspANI GGCC 4 cut(s) 5, 841, 874, 1244
BspCNI CTCAG 2 cut(s) 702, 1016
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 3 cut(s) 1061, 1106, 1169
BspLI GGNNCC 2 cut(s) 103, 1395
BspMI ACCTGC 2 cut(s) 851, 1403
BspPI GGATC 4 cut(s) 1146, 1286, 1388, 1401
BspT104I TTCGAA 1 cut(s) 1226
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 913
BsrI ACTGG 2 cut(s) 1022, 1082
BssECI CCNNGG 5 cut(s) 552, 869, 895, 1295, 1296
BssMI GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
BssSI CACGAG 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 896, 1297
Bst4CI ACNGT 4 cut(s) 218, 797, 982, 1448
BstAPI GCANNNNNTGC 1 cut(s) 1345
BstBI TTCGAA 1 cut(s) 1226
BstC8I GCNNGC 1 cut(s) 1016
BstDEI CTNAG 3 cut(s) 266, 689, 1003
BstF5I GGATG 7 cut(s) 207, 343, 429, 667, 781, 1093, 1180
BstFNI CGCG 3 cut(s) 1061, 1106, 1169
BstKTI GATC 6 cut(s) 117, 165, 1049, 1141, 1294, 1396
BstMAI GTCTC 4 cut(s) 267, 534, 571, 603
BstMBI GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
BstMWI GCNNNNNNNGC 5 cut(s) 186, 1058, 1067, 1345, 1409
BstNI CCWGG 2 cut(s) 896, 1297
BstSCI CCNGG 2 cut(s) 894, 1295
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 1 cut(s) 868
BstUI CGCG 3 cut(s) 1061, 1106, 1169
BstV1I GCAGC 1 cut(s) 1030
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 2 cut(s) 1291, 1393
BstYI RGATCY 2 cut(s) 1291, 1393
BsuRI GGCC 4 cut(s) 5, 841, 874, 1244
BtgZI GCGATG 1 cut(s) 739
BtsCI GGATG 7 cut(s) 207, 343, 429, 667, 781, 1093, 1180
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 3 cut(s) 802, 946, 951
BveI ACCTGC 2 cut(s) 851, 1403
Cac8I GCNNGC 1 cut(s) 1016
Cfr13I GGNCC 1 cut(s) 332
CseI GACGC 2 cut(s) 551, 1067
Csp6I GTAC 5 cut(s) 153, 766, 1108, 1126, 1449
CviAII CATG 6 cut(s) 186, 212, 496, 715, 810, 1246
CviQI GTAC 5 cut(s) 153, 766, 1108, 1126, 1449
DdeI CTNAG 3 cut(s) 266, 689, 1003
DpnI GATC 6 cut(s) 116, 164, 1048, 1140, 1293, 1395
DpnII GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
EaeI YGGCCR 2 cut(s) 3, 1242
Ecl136II GAGCTC 1 cut(s) 1002
Eco130I CCWWGG 1 cut(s) 552
Eco147I AGGCCT 2 cut(s) 841, 874
Eco24I GRGCYC 1 cut(s) 1004
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 1 cut(s) 332
Eco53kI GAGCTC 1 cut(s) 1002
Eco57I CTGAAG 2 cut(s) 450, 531
Eco88I CYCGRG 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 1002
EcoRII CCWGG 2 cut(s) 894, 1295
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 2 cut(s) 65, 1324
EcoT38I GRGCYC 1 cut(s) 1004
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 6 cut(s) 189, 215, 499, 718, 813, 1249
FalI AAGNNNNNCTT 2 cut(s) 1332, 1364
FaqI GGGAC 2 cut(s) 691, 1043
FatI CATG 6 cut(s) 185, 211, 495, 714, 809, 1245
FauI CCCGC 1 cut(s) 1174
FbaI TGATCA 1 cut(s) 114
Fnu4HI GCNGC 2 cut(s) 1019, 1242
FokI GGATG 7 cut(s) 194, 350, 436, 674, 768, 1080, 1187
FriOI GRGCYC 1 cut(s) 1004
Fsp4HI GCNGC 2 cut(s) 1019, 1242
FspBI CTAG 4 cut(s) 41, 279, 492, 1041
GluI GCNGC 2 cut(s) 1019, 1242
HaeIII GGCC 4 cut(s) 5, 841, 874, 1244
HapII CCGG 1 cut(s) 335
HgaI GACGC 2 cut(s) 551, 1067
Hin1II CATG 6 cut(s) 189, 215, 499, 718, 813, 1249
HindIII AAGCTT 1 cut(s) 921
HinfI GANTC 7 cut(s) 182, 193, 260, 521, 704, 1031, 1312
HpaII CCGG 1 cut(s) 335
HphI GGTGA 4 cut(s) 134, 139, 835, 1387
Hpy166II GTNNAC 6 cut(s) 142, 308, 686, 852, 866, 1009
Hpy188I TCNGA 6 cut(s) 352, 367, 539, 650, 673, 999
Hpy188III TCNNGA 5 cut(s) 335, 576, 1316, 1418, 1442
Hpy8I GTNNAC 6 cut(s) 142, 308, 686, 852, 866, 1009
Hpy99I CGWCG 2 cut(s) 533, 1061
HpyAV CCTTC 8 cut(s) 347, 645, 725, 878, 1165, 1187, 1222, 1383
HpyCH4III ACNGT 4 cut(s) 218, 797, 982, 1448
HpyCH4IV ACGT 1 cut(s) 528
HpyF10VI GCNNNNNNNGC 5 cut(s) 186, 1058, 1067, 1345, 1409
HpyF3I CTNAG 3 cut(s) 266, 689, 1003
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 6 cut(s) 189, 215, 499, 718, 813, 1249
Kpn2I TCCGGA 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
LmnI GCTCC 1 cut(s) 101
Lsp1109I GCAGC 1 cut(s) 1030
LweI GCATC 1 cut(s) 1309
MaeI CTAG 4 cut(s) 41, 279, 492, 1041
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 2 cut(s) 43, 197
MalI GATC 6 cut(s) 116, 164, 1048, 1140, 1293, 1395
MboI GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
MboII GAAGA 4 cut(s) 172, 212, 439, 1314
MfeI CAATTG 1 cut(s) 253
MflI RGATCY 2 cut(s) 1291, 1393
MhlI GDGCHC 2 cut(s) 868, 1004
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 253, 319, 759, 937, 1200, 1221, 1436
MluI ACGCGT 1 cut(s) 1059
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 269, 713, 1025, 1306
MmeI TCCRAC 1 cut(s) 390
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 65, 1324
MroI TCCGGA 1 cut(s) 334
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 80, 725, 1307
MslI CAYNNNNRTG 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 896, 1297
MunI CAATTG 1 cut(s) 253
Mva1269I GAATGC 2 cut(s) 1077, 1350
MvaI CCWGG 2 cut(s) 896, 1297
MvnI CGCG 3 cut(s) 1061, 1106, 1169
MwoI GCNNNNNNNGC 5 cut(s) 186, 1058, 1067, 1345, 1409
NdeII GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
NlaIII CATG 6 cut(s) 189, 215, 499, 718, 813, 1249
NlaIV GGNNCC 2 cut(s) 103, 1395
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 2 cut(s) 65, 1324
NspV TTCGAA 1 cut(s) 1226
PaqCI CACCTGC 1 cut(s) 851
PasI CCCWGGG 1 cut(s) 1296
PceI AGGCCT 2 cut(s) 841, 874
PctI GAATGC 2 cut(s) 1077, 1350
PfeI GAWTC 3 cut(s) 182, 193, 521
Pfl23II CGTACG 1 cut(s) 1107
PkrI GCNGC 2 cut(s) 1020, 1243
PleI GAGTC 4 cut(s) 268, 712, 1025, 1306
PpsI GAGTC 4 cut(s) 268, 712, 1025, 1306
PshBI ATTAAT 1 cut(s) 725
Psp124BI GAGCTC 1 cut(s) 1004
Psp6I CCWGG 2 cut(s) 894, 1295
PspGI CCWGG 2 cut(s) 894, 1295
PspLI CGTACG 1 cut(s) 1107
PspN4I GGNNCC 2 cut(s) 103, 1395
PspPI GGNCC 1 cut(s) 332
PsuI RGATCY 2 cut(s) 1291, 1393
RsaI GTAC 5 cut(s) 154, 767, 1109, 1127, 1450
RsaNI GTAC 5 cut(s) 153, 766, 1108, 1126, 1449
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 1004
SaqAI TTAA 3 cut(s) 80, 725, 1307
SatI GCNGC 2 cut(s) 1019, 1242
Sau3AI GATC 6 cut(s) 114, 162, 1046, 1138, 1291, 1393
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 4 cut(s) 269, 713, 1025, 1306
ScrFI CCNGG 2 cut(s) 896, 1297
SduI GDGCHC 2 cut(s) 868, 1004
SfaNI GCATC 1 cut(s) 1309
SfcI CTRYAG 1 cut(s) 786
SfuI TTCGAA 1 cut(s) 1226
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 1418
SmoI CTYRAG 1 cut(s) 1418
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 7 cut(s) 253, 319, 759, 937, 1200, 1221, 1436
SseBI AGGCCT 2 cut(s) 841, 874
SsiI CCGC 3 cut(s) 1104, 1167, 1241
SspI AATATT 1 cut(s) 1118
SspMI CTAG 4 cut(s) 41, 279, 492, 1041
SstI GAGCTC 1 cut(s) 1004
StuI AGGCCT 2 cut(s) 841, 874
StyD4I CCNGG 2 cut(s) 894, 1295
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 218, 797, 982, 1448
TaiI ACGT 1 cut(s) 531
TaqI TCGA 5 cut(s) 531, 575, 909, 1056, 1226
TasI AATT 7 cut(s) 253, 319, 759, 937, 1200, 1221, 1436
TatI WGTACW 1 cut(s) 1448
TauI GCSGC 1 cut(s) 1244
TfiI GAWTC 3 cut(s) 182, 193, 521
Tru1I TTAA 3 cut(s) 80, 725, 1307
Tru9I TTAA 3 cut(s) 80, 725, 1307
TscAI CASTG 3 cut(s) 802, 953, 958
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 1018
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 7 cut(s) 84, 173, 174, 440, 770, 1082, 1394
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 3 cut(s) 802, 953, 958
VneI GTGCAC 1 cut(s) 864
VpaK11BI GGWCC 1 cut(s) 332
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 3 cut(s) 319, 1221, 1436
XspI CTAG 4 cut(s) 41, 279, 492, 1041
Zsp2I ATGCAT 2 cut(s) 65, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.