FvH4_4g13214

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000894
Physical Location & Seq
Reverse (-)
16821379 .. 16826102
4724 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13214.t2

Sequence Viewer

Length: 1485 bp
ATGGGAAAATCATTAGCTGGAAAGGGTTCTCCAGAAGTCTGGTTTGGCATTCTCATCTCTTTTGTTTTATGCTTTGTACTGCTTCCCTTTGATTATCCATGTACTTTTTTTGGGGTAGACTATGGTGTTAATCGCTTTGAAAAGAAACAAACTCACCCAACTGGTATAAAGAACCAGTCGATTTCTGTTATCAATCAATCTACCATAGATTCATGCTCTGGTCGTTACATTTATATACATGATGATCTTCCTGCTAAATTCAACTATGATCTGACCAATAATTGTGAGTCTCTTACTCAAGGGAGTAACATGCGCAATATGTGTCCTTACATAAAGAATTTCGGTTTTGGTCCTGAAATTAACAGCTCTGAAGGGGTTTTAACCAACAAGAGATGGTTCTCCACTAACCAGTTCTCATTAGAGCTCATATTTCACAGCAAAATGAAGCAGTATGAGTGCTTGACAAACAACTCCGCTCAGGCTTCAGCTATTTATATACCAATCTATCCCGGCCTTGAGGACAGTAAACATCTGTGGGATCCTAACCTCACAGCCAGAGACGCTTTCGGTAAAGATTTTGCTAAGTGGGTTTCAAGTAGATCTGAATGGAAGAAAATGTGGGGAAGAGACCATTTCTTTGTTTCAGGAAGGGTTGCTTTTGACTATAGGAGGAAAACTGAAAACATTACTGACTGGGGTAGTAAACTCAGGCTCCTGCCCGAGTCCTGGAACATGACTATGCTAGCAATTGAAGGAAGCTCACTTACAAATGATATTGCTATACCATACCCTACTGGCTTTCATCCTGCACATAACATTGAGGTGTTTCTATGGCAGAACAAAGTGAGGAGGCTAGAAAGGCCTTACTTGTTTACTTTTGTTGGTGGACCAAGGCCTGACCAGCCAAACTCTATCCGGGGCAAACTTTTCGATCAGTGTCAGGCTTCAAACTATTGCATAGGATTAAATTGCAAATCAAGGGGGATCAATTGTGAAGAAACGGTTACTACTATGAGGGTGTTTCAGAAATCAGTATACTGCTTGCAGCCTACTGGGGATACATACACCAGAAAAGCAGCTTTCGACTCGATTCTGGCTGGTTGCATTCCTGTTTTCTTTCATCCGGCTAGCGCTTATAACCAGTACTTATGGTACTTGCCAAAGAATTACACCAAGTATTCTGTTTTTATTCCAGTTAGGAGAGTAAGAGATTTGGAAGACGGTGACATTGAGAAAATCTTGCTTGGAATTTCAAAGGATAGGAAAGTCGCGATGAGAGAGCAAGTGATAAGGCTAATACCAAAGTTGGTGTATGGAGATCCAAGGTCAAGATTAGAGACCAAGGATGCATTTGATATTGCTGTCAACAAAATACTTGAGAGGATAGAGAACGTAAGGAAGCTGATTGGGGAAGGGCGGGATCCTAGTATTGGATTTGCAGAGCAGGATGGTAACAAGTTCATGTTTCCAAAGGAGCTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

495

Amino Acids

56.77

Weight (kDa)

8.88

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 71 - 405 7.3e-73 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1137
Acc16I TGCGCA 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 476
AccI GTMKAC 2 cut(s) 117, 1035
AccII CGCG 1 cut(s) 1271
AciI CCGC 2 cut(s) 474, 1417
AclWI GGATC 6 cut(s) 533, 546, 992, 1313, 1415, 1428
AcsI RAATTY 3 cut(s) 257, 337, 1248
AcuI CTGAAG 2 cut(s) 390, 468
AfaI GTAC 4 cut(s) 78, 103, 1145, 1154
AfeI AGCGCT 1 cut(s) 1132
AfiI CCNNNNNNNGG 2 cut(s) 726, 1430
AgsI TTSAA 6 cut(s) 140, 262, 594, 752, 948, 1254
AjnI CCWGG 1 cut(s) 725
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 8 cut(s) 17, 366, 424, 488, 759, 1079, 1402, 1477
AluI AGCT 8 cut(s) 17, 366, 424, 488, 759, 1079, 1402, 1477
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 4 cut(s) 294, 552, 621, 1331
AlwI GGATC 6 cut(s) 533, 546, 992, 1313, 1415, 1428
Ama87I CYCGRG 1 cut(s) 719
Aor51HI AGCGCT 1 cut(s) 1132
AoxI GGCC 3 cut(s) 511, 860, 893
ApeKI GCWGC 2 cut(s) 1045, 1076
ApoI RAATTY 3 cut(s) 257, 337, 1248
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 2 cut(s) 315, 1133
AspS9I GGNCC 2 cut(s) 350, 887
AsuC2I CCSGG 2 cut(s) 510, 917
AsuHPI GGTGA 2 cut(s) 146, 1235
AsuNHI GCTAGC 2 cut(s) 742, 1127
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 2 cut(s) 350, 887
BamHI GGATCC 2 cut(s) 538, 1420
BanII GRGCYC 1 cut(s) 426
BarI GAAGNNNNNNTAC 2 cut(s) 748, 780
BbsI GAAGAC 1 cut(s) 1224
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 1057, 1088
BccI CCATC 2 cut(s) 387, 1442
BcgI CGANNNNNNTGC 2 cut(s) 910, 944
BciT130I CCWGG 1 cut(s) 727
BciVI GTATCC 1 cut(s) 1051
BcnI CCSGG 2 cut(s) 510, 917
BcoDI GTCTC 4 cut(s) 294, 552, 621, 1331
BfaI CTAG 5 cut(s) 743, 854, 1128, 1425, 1478
BfmI CTRYAG 1 cut(s) 664
BfoI RGCGCY 1 cut(s) 1134
BfuI GTATCC 1 cut(s) 1051
BglII AGATCT 1 cut(s) 599
BisI GCNGC 2 cut(s) 1046, 1077
BlsI GCNGC 2 cut(s) 1047, 1078
BmcAI AGTACT 1 cut(s) 1145
Bme1390I CCNGG 3 cut(s) 510, 727, 917
Bme18I GGWCC 2 cut(s) 350, 887
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 2 cut(s) 350, 887
BmiI GGNNCC 3 cut(s) 540, 713, 1422
BmrFI CCNGG 3 cut(s) 510, 727, 917
BmrI ACTGGG 2 cut(s) 703, 1062
BmsI GCATC 1 cut(s) 1336
BmtI GCTAGC 2 cut(s) 746, 1131
BmuI ACTGGG 2 cut(s) 703, 1062
BpiI GAAGAC 1 cut(s) 1224
BpmI CTGGAG 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 477
BpuEI CTTGAG 3 cut(s) 282, 536, 1397
BpuMI CCSGG 2 cut(s) 510, 917
BsaI GGTCTC 2 cut(s) 621, 1331
BsaJI CCNNGG 4 cut(s) 890, 916, 1322, 1341
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 2 cut(s) 726, 1430
Bse1I ACTGG 8 cut(s) 166, 175, 409, 698, 799, 1057, 1141, 1193
BseBI CCWGG 1 cut(s) 727
BseDI CCNNGG 4 cut(s) 890, 916, 1322, 1341
BseGI GGATG 4 cut(s) 802, 1120, 1351, 1453
BseLI CCNNNNNNNGG 2 cut(s) 726, 1430
BseMII CTCAG 2 cut(s) 491, 721
BseNI ACTGG 8 cut(s) 166, 175, 409, 698, 799, 1057, 1141, 1193
BseRI GAGGAG 1 cut(s) 862
BseXI GCAGC 2 cut(s) 1057, 1088
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 1 cut(s) 1271
BshFI GGCC 3 cut(s) 513, 862, 895
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 3 cut(s) 510, 916, 1124
BslI CCNNNNNNNGG 2 cut(s) 726, 1430
BsmAI GTCTC 4 cut(s) 294, 552, 621, 1331
BsmBI CGTCTC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 48, 1104
BsnI GGCC 3 cut(s) 513, 862, 895
Bso31I GGTCTC 2 cut(s) 621, 1331
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
Bsp68I TCGCGA 1 cut(s) 1271
BspACI CCGC 2 cut(s) 474, 1417
BspANI GGCC 3 cut(s) 513, 862, 895
BspCNI CTCAG 2 cut(s) 490, 720
BspFNI CGCG 1 cut(s) 1271
BspLI GGNNCC 3 cut(s) 540, 713, 1422
BspOI GCTAGC 2 cut(s) 746, 1131
BspPI GGATC 6 cut(s) 533, 546, 992, 1313, 1415, 1428
BspTNI GGTCTC 2 cut(s) 621, 1331
BsrBI CCGCTC 1 cut(s) 476
BsrI ACTGG 8 cut(s) 166, 175, 409, 698, 799, 1057, 1141, 1193
BssECI CCNNGG 4 cut(s) 890, 916, 1322, 1341
BssMI GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
BssNAI GTATAC 1 cut(s) 1036
BssT1I CCWWGG 3 cut(s) 890, 1322, 1341
Bst1107I GTATAC 1 cut(s) 1036
Bst2UI CCWGG 1 cut(s) 727
Bst4CI ACNGT 3 cut(s) 524, 1003, 1223
Bst6I CTCTTC 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 744, 1043, 1129
BstDEI CTNAG 3 cut(s) 477, 582, 707
BstF5I GGATG 4 cut(s) 802, 1120, 1351, 1453
BstFNI CGCG 1 cut(s) 1271
BstH2I RGCGCY 1 cut(s) 1134
BstHHI GCGC 2 cut(s) 315, 1133
BstKTI GATC 8 cut(s) 247, 271, 541, 602, 934, 987, 1321, 1423
BstMAI GTCTC 4 cut(s) 294, 552, 621, 1331
BstMBI GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
BstMWI GCNNNNNNNGC 3 cut(s) 560, 859, 901
BstNI CCWGG 1 cut(s) 727
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 3 cut(s) 508, 725, 915
BstSFI CTRYAG 1 cut(s) 664
BstUI CGCG 1 cut(s) 1271
BstV1I GCAGC 2 cut(s) 1057, 1088
BstV2I GAAGAC 1 cut(s) 1224
BstX2I RGATCY 4 cut(s) 538, 599, 1318, 1420
BstXI CCANNNNNNTGG 1 cut(s) 39
BstYI RGATCY 4 cut(s) 538, 599, 1318, 1420
BstZ17I GTATAC 1 cut(s) 1036
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 3 cut(s) 513, 862, 895
BtgZI GCGATG 1 cut(s) 1286
BtsCI GGATG 4 cut(s) 802, 1120, 1351, 1453
BtsIMutI CAGTG 1 cut(s) 941
BtuMI TCGCGA 1 cut(s) 1271
Cac8I GCNNGC 3 cut(s) 744, 1043, 1129
CfoI GCGC 2 cut(s) 315, 1133
Cfr13I GGNCC 2 cut(s) 350, 887
CseI GACGC 1 cut(s) 569
Csp6I GTAC 4 cut(s) 77, 102, 1144, 1153
CviAII CATG 6 cut(s) 99, 213, 239, 310, 733, 1462
CviQI GTAC 4 cut(s) 77, 102, 1144, 1153
DdeI CTNAG 3 cut(s) 477, 582, 707
DpnI GATC 8 cut(s) 246, 270, 540, 601, 933, 986, 1320, 1422
DpnII GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
Eam1104I CTCTTC 1 cut(s) 619
EarI CTCTTC 1 cut(s) 619
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 3 cut(s) 890, 1322, 1341
Eco147I AGGCCT 2 cut(s) 862, 895
Eco24I GRGCYC 1 cut(s) 426
Eco31I GGTCTC 2 cut(s) 621, 1331
Eco47I GGWCC 2 cut(s) 350, 887
Eco47III AGCGCT 1 cut(s) 1132
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 2 cut(s) 390, 468
Eco88I CYCGRG 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 725
EcoT14I CCWWGG 3 cut(s) 890, 1322, 1341
EcoT22I ATGCAT 1 cut(s) 1351
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 3 cut(s) 890, 1322, 1341
Esp3I CGTCTC 1 cut(s) 552
FaeI CATG 6 cut(s) 102, 216, 242, 313, 736, 1465
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FatI CATG 6 cut(s) 98, 212, 238, 309, 732, 1461
FauI CCCGC 1 cut(s) 1410
FblI GTMKAC 2 cut(s) 117, 1035
Fnu4HI GCNGC 2 cut(s) 1046, 1077
FokI GGATG 4 cut(s) 789, 1107, 1358, 1460
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 2 cut(s) 1046, 1077
FspBI CTAG 5 cut(s) 743, 854, 1128, 1425, 1478
FspI TGCGCA 1 cut(s) 314
GlaI GCGC 2 cut(s) 314, 1132
GluI GCNGC 2 cut(s) 1046, 1077
GsuI CTGGAG 1 cut(s) 15
HaeII RGCGCY 1 cut(s) 1134
HaeIII GGCC 3 cut(s) 513, 862, 895
HapII CCGG 3 cut(s) 510, 916, 1124
HgaI GACGC 1 cut(s) 569
HhaI GCGC 2 cut(s) 315, 1133
Hin1II CATG 6 cut(s) 102, 216, 242, 313, 736, 1465
Hin6I GCGC 2 cut(s) 313, 1131
HinP1I GCGC 2 cut(s) 313, 1131
HincII GTYRAC 1 cut(s) 1366
HindII GTYRAC 1 cut(s) 1366
HinfI GANTC 5 cut(s) 209, 287, 722, 1085, 1090
HpaII CCGG 3 cut(s) 510, 916, 1124
HphI GGTGA 2 cut(s) 146, 1235
Hpy166II GTNNAC 7 cut(s) 118, 527, 704, 873, 887, 1036, 1366
Hpy188I TCNGA 4 cut(s) 273, 370, 604, 1026
Hpy188III TCNNGA 5 cut(s) 32, 353, 645, 1270, 1329
Hpy8I GTNNAC 7 cut(s) 118, 527, 704, 873, 887, 1036, 1366
HpyAV CCTTC 4 cut(s) 365, 642, 746, 1406
HpyCH4III ACNGT 3 cut(s) 524, 1003, 1223
HpyCH4IV ACGT 1 cut(s) 1392
HpyCH4V TGCA 7 cut(s) 809, 957, 972, 1045, 1104, 1349, 1439
HpyF10VI GCNNNNNNNGC 3 cut(s) 560, 859, 901
HpyF3I CTNAG 3 cut(s) 477, 582, 707
HpySE526I ACGT 1 cut(s) 1392
Hsp92II CATG 6 cut(s) 102, 216, 242, 313, 736, 1465
HspAI GCGC 2 cut(s) 313, 1131
Kzo9I GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
LmnI GCTCC 2 cut(s) 717, 1474
Lsp1109I GCAGC 2 cut(s) 1057, 1088
LweI GCATC 1 cut(s) 1336
MaeI CTAG 5 cut(s) 743, 854, 1128, 1425, 1478
MaeII ACGT 1 cut(s) 1392
MaeIII GTNAC 5 cut(s) 224, 305, 1003, 1223, 1451
MalI GATC 8 cut(s) 246, 270, 540, 601, 933, 986, 1320, 1422
MbiI CCGCTC 1 cut(s) 476
MboI GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
MboII GAAGA 5 cut(s) 239, 622, 636, 1007, 1229
MfeI CAATTG 2 cut(s) 747, 988
MflI RGATCY 4 cut(s) 538, 599, 1318, 1420
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 9 cut(s) 257, 280, 337, 357, 747, 967, 988, 1165, 1248
MlyI GAGTC 3 cut(s) 296, 731, 1079
MnlI CCTC 8 cut(s) 511, 557, 663, 814, 840, 843, 1008, 1374
Mph1103I ATGCAT 1 cut(s) 1351
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 4 cut(s) 129, 360, 380, 965
MslI CAYNNNNRTG 2 cut(s) 737, 821
MspI CCGG 3 cut(s) 510, 916, 1124
MspR9I CCNGG 3 cut(s) 510, 727, 917
MunI CAATTG 2 cut(s) 747, 988
Mva1269I GAATGC 2 cut(s) 48, 1104
MvaI CCWGG 1 cut(s) 727
MvnI CGCG 1 cut(s) 1271
MwoI GCNNNNNNNGC 3 cut(s) 560, 859, 901
NciI CCSGG 2 cut(s) 510, 917
NdeII GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
NheI GCTAGC 2 cut(s) 742, 1127
NlaIII CATG 6 cut(s) 102, 216, 242, 313, 736, 1465
NlaIV GGNNCC 3 cut(s) 540, 713, 1422
NmuCI GTSAC 1 cut(s) 1223
NruI TCGCGA 1 cut(s) 1271
NsbI TGCGCA 1 cut(s) 314
NsiI ATGCAT 1 cut(s) 1351
NspI RCATGY 1 cut(s) 313
PceI AGGCCT 2 cut(s) 862, 895
PctI GAATGC 2 cut(s) 48, 1104
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 2 cut(s) 209, 1090
PfoI TCCNGGA 1 cut(s) 725
PkrI GCNGC 2 cut(s) 1047, 1078
PleI GAGTC 3 cut(s) 295, 730, 1079
PpsI GAGTC 3 cut(s) 295, 730, 1079
PsiI TTATAA 1 cut(s) 1137
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 725
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 3 cut(s) 540, 713, 1422
PspPI GGNCC 2 cut(s) 350, 887
PsuI RGATCY 4 cut(s) 538, 599, 1318, 1420
RruI TCGCGA 1 cut(s) 1271
RsaI GTAC 4 cut(s) 78, 103, 1145, 1154
RsaNI GTAC 4 cut(s) 77, 102, 1144, 1153
RseI CAYNNNNRTG 2 cut(s) 737, 821
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 4 cut(s) 129, 360, 380, 965
SatI GCNGC 2 cut(s) 1046, 1077
Sau3AI GATC 8 cut(s) 244, 268, 538, 599, 931, 984, 1318, 1420
Sau96I GGNCC 2 cut(s) 350, 887
ScaI AGTACT 1 cut(s) 1145
SchI GAGTC 3 cut(s) 296, 731, 1079
ScrFI CCNGG 3 cut(s) 510, 727, 917
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 1 cut(s) 1336
SfcI CTRYAG 1 cut(s) 664
SinI GGWCC 2 cut(s) 350, 887
SmiMI CAYNNNNRTG 2 cut(s) 737, 821
SmlI CTYRAG 3 cut(s) 297, 515, 1376
SmoI CTYRAG 3 cut(s) 297, 515, 1376
Sse9I AATT 9 cut(s) 257, 280, 337, 357, 747, 967, 988, 1165, 1248
SseBI AGGCCT 2 cut(s) 862, 895
SsiI CCGC 2 cut(s) 474, 1417
SspMI CTAG 5 cut(s) 743, 854, 1128, 1425, 1478
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 2 cut(s) 862, 895
StyD4I CCNGG 3 cut(s) 508, 725, 915
StyI CCWWGG 3 cut(s) 890, 1322, 1341
TaaI ACNGT 3 cut(s) 524, 1003, 1223
TaiI ACGT 1 cut(s) 1395
TaqI TCGA 4 cut(s) 179, 930, 1083, 1088
TasI AATT 9 cut(s) 257, 280, 337, 357, 747, 967, 988, 1165, 1248
TatI WGTACW 3 cut(s) 76, 101, 1143
TfiI GAWTC 2 cut(s) 209, 1090
Tru1I TTAA 4 cut(s) 129, 360, 380, 965
Tru9I TTAA 4 cut(s) 129, 360, 380, 965
TscAI CASTG 1 cut(s) 941
TseFI GTSAC 1 cut(s) 1223
TseI GCWGC 2 cut(s) 1045, 1076
Tsp45I GTSAC 1 cut(s) 1223
TspDTI ATGAA 5 cut(s) 201, 458, 791, 1109, 1450
TspRI CASTG 1 cut(s) 941
VpaK11BI GGWCC 2 cut(s) 350, 887
XapI RAATTY 3 cut(s) 257, 337, 1248
XceI RCATGY 1 cut(s) 313
XmiI GTMKAC 2 cut(s) 117, 1035
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 5 cut(s) 743, 854, 1128, 1425, 1478
ZrmI AGTACT 1 cut(s) 1145
Zsp2I ATGCAT 1 cut(s) 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.