MD04G1017900.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000894
Physical Location & Seq
Reverse (-)
2076252 .. 2077803
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1017900.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGCACGACGATCTTCCTGAAAAATTCAACTCCGACTTGGTAAGAAACTGTAGGTTTCTCACCAGAGGAACCGACAAGCCAAATTTATGTATGTACTTTGAGAATTATGGTTTCGGTCCCGAAATCCAAAACTCGGAAGGGGTTTTGTCGAACGGGAGCTGGTTTTCCACGAACCAGTTCTTGCTGGAGTTCATATTCCACAACAAGATGAAGCGCTATAAGTGTTTGACAAACATCTCCAGTCAGGCTTCGGCCGTTTATGTCCCGTTTTACGCCGGCCTTGATGCGAGCCTCCATCTCTGGGACTCGAATCTTACGGTTAGAGATGCCTCGGCGAAAGAACTTGTGAGTTGGCTTGCGAGAAAACCCGAGTGGGGGAAACTGTGGGGGAGGGATCATTTCTTGGTTGCCGGGAGAATTTCTTGGGACTTCAGGAGGCAGACGGATGAGGTTTCGGATTGGGGTAGTAAGCTCAGGTGGTTGCCTGAGTCTATGAACATGACTATGTTGTCGGTCGAAGGAAGCTCTTGGAACAATGACTTTGCTATACCTTACCCGACAAATTTTCATCCCGCGGAGGAGGTCGATTTGGTTCAATGGCAGAACAGAATGAGGAAGCTAGACAGGCCTTACTTGTTCACTTTTGCTGGGGCACCGAGGCCTGACCAGCAGAGCTCGATTCGGGGGAAGATTATCGATCAGTGCGTAGCTTCGAGTAGATGCAAGTTAATCGATTGCAGTTTGGGCGGTAACATGGATTGTGACAACCCGTCGAGTGTTATGAGGGTGTTTCAGAGCTCGGTTTATTGCTTGCAGCCCGGAGGGGATTCATACACCAGGAGATCAACTTTCGATTCCATTCTGGCTGGTTGTATTCCGGTTTTCTTTCACCCGGGTGCCGCTTATTCCCAGTATTTGTGGCATTTGCCGAAGAATCATAGCAGCTACTCGGTGTACATTCCGGTGAGGGTTGCGGACGATATGCCAGGCAGCATTGAGGAAATCTTGCTTGGAATTTCAAAGGATAAGGAATTAGAGACCGAAGATGCATTCGATTTGGCTATCAAGGGAATTCTTGAGAGGATTGAGGATGTTAGGAAGGTGATTAGGGAAGGGAATGATCCGAGTATTGGTTTCGCGGATGAGGATAGCTACAAGTACACATTTCATCAGAAACAAGAACAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

397

Amino Acids

45.42

Weight (kDa)

5.56

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 1 - 332 1.1e-76 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 508
AccB1I GGYRCC 2 cut(s) 652, 896
AccII CGCG 2 cut(s) 575, 1139
AciI CCGC 6 cut(s) 573, 575, 747, 900, 974, 1139
AclWI GGATC 2 cut(s) 402, 1115
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 6 cut(s) 23, 82, 417, 562, 1014, 1071
AcuI CTGAAG 1 cut(s) 415
AfaI GTAC 3 cut(s) 95, 956, 1160
AfeI AGCGCT 1 cut(s) 215
AfiI CCNNNNNNNGG 5 cut(s) 133, 301, 374, 375, 1130
AgsI TTSAA 3 cut(s) 28, 596, 1020
AhdI GACNNNNNGTC 1 cut(s) 769
AjnI CCWGG 2 cut(s) 836, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
AleI CACNNNNGTG 1 cut(s) 894
AluBI AGCT 9 cut(s) 159, 472, 525, 619, 675, 710, 798, 945, 1152
AluI AGCT 9 cut(s) 159, 472, 525, 619, 675, 710, 798, 945, 1152
Alw21I GWGCWC 2 cut(s) 677, 800
Alw26I GTCTC 1 cut(s) 1031
AlwI GGATC 2 cut(s) 402, 1115
Ama87I CYCGRG 2 cut(s) 368, 892
Aor51HI AGCGCT 1 cut(s) 215
AoxI GGCC 4 cut(s) 252, 277, 626, 659
ApeKI GCWGC 3 cut(s) 814, 942, 990
ApoI RAATTY 6 cut(s) 23, 82, 417, 562, 1014, 1071
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 4 cut(s) 412, 819, 893, 894
AsuHPI GGTGA 4 cut(s) 52, 881, 976, 1114
AvaI CYCGRG 2 cut(s) 368, 892
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 655
BanI GGYRCC 2 cut(s) 652, 896
BanII GRGCYC 2 cut(s) 677, 800
Bbv12I GWGCWC 2 cut(s) 677, 800
BbvI GCAGC 3 cut(s) 826, 954, 1002
BccI CCATC 1 cut(s) 303
BceAI ACGGC 1 cut(s) 239
BcgI CGANNNNNNTGC 2 cut(s) 712, 746
BciT130I CCWGG 2 cut(s) 838, 987
BcnI CCSGG 4 cut(s) 412, 819, 893, 894
BcoDI GTCTC 1 cut(s) 1031
BfaI CTAG 1 cut(s) 620
BfmI CTRYAG 1 cut(s) 49
BfoI RGCGCY 1 cut(s) 217
BisI GCNGC 4 cut(s) 815, 900, 943, 991
BlsI GCNGC 4 cut(s) 816, 901, 944, 992
Bme1390I CCNGG 6 cut(s) 412, 819, 838, 893, 894, 987
Bme18I GGWCC 1 cut(s) 116
BmeRI GACNNNNNGTC 1 cut(s) 769
BmeT110I CYCGRG 2 cut(s) 368, 892
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 4 cut(s) 70, 118, 654, 898
BmrFI CCNGG 6 cut(s) 412, 819, 838, 893, 894, 987
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 4 cut(s) 274, 316, 710, 1036
BmuI ACTGGG 1 cut(s) 904
BpmI CTGGAG 2 cut(s) 206, 223
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 1097
BpuMI CCSGG 4 cut(s) 412, 819, 893, 894
Bsa29I ATCGAT 2 cut(s) 696, 732
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 4 cut(s) 330, 573, 656, 892
BsaWI WCCGGW 2 cut(s) 877, 961
Bsc4I CCNNNNNNNGG 5 cut(s) 133, 301, 374, 375, 1130
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 3 cut(s) 175, 240, 910
BseBI CCWGG 2 cut(s) 838, 987
BseCI ATCGAT 2 cut(s) 696, 732
BseDI CCNNGG 4 cut(s) 330, 573, 656, 892
BseGI GGATG 4 cut(s) 451, 568, 1096, 1147
BseLI CCNNNNNNNGG 5 cut(s) 133, 301, 374, 375, 1130
BseMII CTCAG 2 cut(s) 477, 487
BseNI ACTGG 3 cut(s) 175, 240, 910
BseRI GAGGAG 1 cut(s) 593
BseSI GKGCMC 1 cut(s) 655
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 3 cut(s) 826, 954, 1002
BseYI CCCAGC 1 cut(s) 647
Bsh1236I CGCG 2 cut(s) 575, 1139
Bsh1285I CGRYCG 2 cut(s) 255, 516
BshFI GGCC 4 cut(s) 254, 279, 628, 661
BshNI GGYRCC 2 cut(s) 652, 896
BshVI ATCGAT 2 cut(s) 696, 732
BsiEI CGRYCG 2 cut(s) 255, 516
BsiHKAI GWGCWC 2 cut(s) 677, 800
BsiHKCI CYCGRG 2 cut(s) 368, 892
BsiSI CCGG 6 cut(s) 276, 411, 819, 878, 893, 962
BslFI GGGAC 4 cut(s) 102, 248, 317, 440
BslI CCNNNNNNNGG 5 cut(s) 133, 301, 374, 375, 1130
BsmAI GTCTC 1 cut(s) 1031
BsmFI GGGAC 4 cut(s) 102, 248, 317, 440
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 4 cut(s) 254, 279, 628, 661
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 2 cut(s) 368, 892
Bsp1286I GDGCHC 3 cut(s) 655, 677, 800
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 5 cut(s) 10, 394, 697, 842, 1120
BspACI CCGC 6 cut(s) 573, 575, 747, 900, 974, 1139
BspANI GGCC 4 cut(s) 254, 279, 628, 661
BspCNI CTCAG 2 cut(s) 478, 486
BspDI ATCGAT 2 cut(s) 696, 732
BspFNI CGCG 2 cut(s) 575, 1139
BspLI GGNNCC 4 cut(s) 70, 118, 654, 898
BspPI GGATC 2 cut(s) 402, 1115
BspT107I GGYRCC 2 cut(s) 652, 896
BspTNI GGTCTC 1 cut(s) 1031
BsrFI RCCGGY 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 3 cut(s) 175, 240, 910
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 330, 573, 656, 892
BssMI GATC 5 cut(s) 10, 394, 697, 842, 1120
Bst2UI CCWGG 2 cut(s) 838, 987
Bst4CI ACNGT 3 cut(s) 50, 319, 384
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 4 cut(s) 277, 289, 357, 812
BstDEI CTNAG 2 cut(s) 473, 486
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 4 cut(s) 451, 568, 1096, 1147
BstFNI CGCG 2 cut(s) 575, 1139
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 5 cut(s) 13, 397, 700, 845, 1123
BstMAI GTCTC 1 cut(s) 1031
BstMBI GATC 5 cut(s) 10, 394, 697, 842, 1120
BstMCI CGRYCG 2 cut(s) 255, 516
BstMWI GCNNNNNNNGC 3 cut(s) 625, 667, 744
BstNI CCWGG 2 cut(s) 838, 987
BstSCI CCNGG 6 cut(s) 410, 817, 836, 891, 892, 985
BstSFI CTRYAG 1 cut(s) 49
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 2 cut(s) 575, 1139
BstV1I GCAGC 3 cut(s) 826, 954, 1002
BstZI CGGCCG 1 cut(s) 252
Bsu15I ATCGAT 2 cut(s) 696, 732
BsuRI GGCC 4 cut(s) 254, 279, 628, 661
BsuTUI ATCGAT 2 cut(s) 696, 732
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 4 cut(s) 451, 568, 1096, 1147
BtsIMutI CAGTG 1 cut(s) 707
Cac8I GCNNGC 4 cut(s) 277, 289, 357, 812
CfoI GCGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 116
Cfr42I CCGCGG 1 cut(s) 576
Cfr9I CCCGGG 1 cut(s) 892
ClaI ATCGAT 2 cut(s) 696, 732
Csp6I GTAC 3 cut(s) 94, 955, 1159
CviAII CATG 2 cut(s) 499, 754
CviQI GTAC 3 cut(s) 94, 955, 1159
DdeI CTNAG 2 cut(s) 473, 486
DpnI GATC 5 cut(s) 12, 396, 699, 844, 1122
DpnII GATC 5 cut(s) 10, 394, 697, 842, 1120
DrdI GACNNNNNNGTC 1 cut(s) 508
DriI GACNNNNNGTC 1 cut(s) 769
DseDI GACNNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 252
EagI CGGCCG 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 769
Ecl136II GAGCTC 2 cut(s) 675, 798
EclXI CGGCCG 1 cut(s) 252
Eco147I AGGCCT 2 cut(s) 628, 661
Eco24I GRGCYC 2 cut(s) 677, 800
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 1 cut(s) 116
Eco47III AGCGCT 1 cut(s) 215
Eco52I CGGCCG 1 cut(s) 252
Eco53kI GAGCTC 2 cut(s) 675, 798
Eco57I CTGAAG 1 cut(s) 415
Eco88I CYCGRG 2 cut(s) 368, 892
EcoICRI GAGCTC 2 cut(s) 675, 798
EcoRI GAATTC 1 cut(s) 1071
EcoRII CCWGG 2 cut(s) 836, 985
EcoT22I ATGCAT 1 cut(s) 1051
EcoT38I GRGCYC 2 cut(s) 677, 800
FaeI CATG 2 cut(s) 502, 757
FaqI GGGAC 4 cut(s) 102, 248, 317, 440
FatI CATG 2 cut(s) 498, 753
FauI CCCGC 1 cut(s) 580
Fnu4HI GCNGC 4 cut(s) 815, 900, 943, 991
FokI GGATG 4 cut(s) 458, 555, 1103, 1154
FriOI GRGCYC 2 cut(s) 677, 800
Fsp4HI GCNGC 4 cut(s) 815, 900, 943, 991
FspBI CTAG 1 cut(s) 620
GlaI GCGC 1 cut(s) 215
GluI GCNGC 4 cut(s) 815, 900, 943, 991
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 2 cut(s) 206, 223
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 4 cut(s) 254, 279, 628, 661
HapII CCGG 6 cut(s) 276, 411, 819, 878, 893, 962
HhaI GCGC 1 cut(s) 216
Hin1II CATG 2 cut(s) 502, 757
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HinfI GANTC 7 cut(s) 305, 310, 488, 679, 827, 854, 934
HpaII CCGG 6 cut(s) 276, 411, 819, 878, 893, 962
HphI GGTGA 4 cut(s) 52, 881, 976, 1114
Hpy166II GTNNAC 3 cut(s) 639, 955, 1161
Hpy188I TCNGA 6 cut(s) 34, 136, 457, 795, 1125, 1173
Hpy188III TCNNGA 4 cut(s) 17, 119, 433, 1076
Hpy8I GTNNAC 3 cut(s) 639, 955, 1161
Hpy99I CGWCG 2 cut(s) 11, 775
HpyAV CCTTC 4 cut(s) 131, 512, 1093, 1106
HpyCH4III ACNGT 3 cut(s) 50, 319, 384
HpyCH4V TGCA 5 cut(s) 4, 723, 738, 814, 1049
HpyF10VI GCNNNNNNNGC 3 cut(s) 625, 667, 744
HpyF3I CTNAG 2 cut(s) 473, 486
Hsp92II CATG 2 cut(s) 502, 757
HspAI GCGC 1 cut(s) 214
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
KspI CCGCGG 1 cut(s) 576
Kzo9I GATC 5 cut(s) 10, 394, 697, 842, 1120
LmnI GCTCC 1 cut(s) 156
Lsp1109I GCAGC 3 cut(s) 826, 954, 1002
LweI GCATC 4 cut(s) 274, 316, 710, 1036
MaeI CTAG 1 cut(s) 620
MaeIII GTNAC 2 cut(s) 749, 761
MalI GATC 5 cut(s) 12, 396, 699, 844, 1122
MboI GATC 5 cut(s) 10, 394, 697, 842, 1120
MboII GAAGA 4 cut(s) 5, 700, 943, 1055
MhlI GDGCHC 3 cut(s) 655, 677, 800
MluCI AATT 8 cut(s) 23, 82, 103, 417, 562, 1014, 1031, 1071
MlyI GAGTC 2 cut(s) 299, 497
MmeI TCCRAC 1 cut(s) 57
Mph1103I ATGCAT 1 cut(s) 1051
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 1 cut(s) 728
MslI CAYNNNNRTG 3 cut(s) 503, 894, 962
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 6 cut(s) 276, 411, 819, 878, 893, 962
MspR9I CCNGG 6 cut(s) 412, 819, 838, 893, 894, 987
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 2 cut(s) 838, 987
MvnI CGCG 2 cut(s) 575, 1139
MwoI GCNNNNNNNGC 3 cut(s) 625, 667, 744
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 4 cut(s) 412, 819, 893, 894
NdeII GATC 5 cut(s) 10, 394, 697, 842, 1120
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 2 cut(s) 502, 757
NlaIV GGNNCC 4 cut(s) 70, 118, 654, 898
NmeAIII GCCGAG 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 761
NsiI ATGCAT 1 cut(s) 1051
OliI CACNNNNGTG 1 cut(s) 894
PceI AGGCCT 2 cut(s) 628, 661
PcsI WCGNNNNNNNCGW 1 cut(s) 702
PctI GAATGC 1 cut(s) 1049
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 5 cut(s) 310, 679, 827, 854, 934
PkrI GCNGC 4 cut(s) 816, 901, 944, 992
PleI GAGTC 2 cut(s) 299, 496
PpsI GAGTC 2 cut(s) 299, 496
Psp124BI GAGCTC 2 cut(s) 677, 800
Psp6I CCWGG 2 cut(s) 836, 985
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 2 cut(s) 836, 985
PspN4I GGNNCC 4 cut(s) 70, 118, 654, 898
PspPI GGNCC 1 cut(s) 116
RsaI GTAC 3 cut(s) 95, 956, 1160
RsaNI GTAC 3 cut(s) 94, 955, 1159
RseI CAYNNNNRTG 3 cut(s) 503, 894, 962
SacI GAGCTC 2 cut(s) 677, 800
SacII CCGCGG 1 cut(s) 576
SaqAI TTAA 1 cut(s) 728
SatI GCNGC 4 cut(s) 815, 900, 943, 991
Sau3AI GATC 5 cut(s) 10, 394, 697, 842, 1120
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 2 cut(s) 299, 497
ScrFI CCNGG 6 cut(s) 412, 819, 838, 893, 894, 987
SduI GDGCHC 3 cut(s) 655, 677, 800
SfaNI GCATC 4 cut(s) 274, 316, 710, 1036
SfcI CTRYAG 1 cut(s) 49
Sfr303I CCGCGG 1 cut(s) 576
SgrBI CCGCGG 1 cut(s) 576
SinI GGWCC 1 cut(s) 116
SmaI CCCGGG 1 cut(s) 894
SmiMI CAYNNNNRTG 3 cut(s) 503, 894, 962
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
Sse9I AATT 8 cut(s) 23, 82, 103, 417, 562, 1014, 1031, 1071
SseBI AGGCCT 2 cut(s) 628, 661
SsiI CCGC 6 cut(s) 573, 575, 747, 900, 974, 1139
SspMI CTAG 1 cut(s) 620
SstI GAGCTC 2 cut(s) 677, 800
StuI AGGCCT 2 cut(s) 628, 661
StyD4I CCNGG 6 cut(s) 410, 817, 836, 891, 892, 985
TaaI ACNGT 3 cut(s) 50, 319, 384
TaqII GACCGA 3 cut(s) 104, 502, 1055
TasI AATT 8 cut(s) 23, 82, 103, 417, 562, 1014, 1031, 1071
TatI WGTACW 3 cut(s) 93, 954, 1158
TauI GCSGC 1 cut(s) 902
TfiI GAWTC 5 cut(s) 310, 679, 827, 854, 934
Tru1I TTAA 1 cut(s) 728
Tru9I TTAA 1 cut(s) 728
TscAI CASTG 1 cut(s) 707
TseFI GTSAC 1 cut(s) 761
TseI GCWGC 3 cut(s) 814, 942, 990
Tsp45I GTSAC 1 cut(s) 761
TspDTI ATGAA 6 cut(s) 181, 224, 509, 557, 819, 1157
TspGWI ACGGA 1 cut(s) 458
TspMI CCCGGG 1 cut(s) 892
TspRI CASTG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 6 cut(s) 23, 82, 417, 562, 1014, 1071
XmaI CCCGGG 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 620
Zsp2I ATGCAT 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.