Rroxscaffold_5G00357320

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000894
Physical Location & Seq
Reverse (-)
36935369 .. 36941965
6597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00357320.1

Sequence Viewer

Length: 1560 bp
ATGACTGCTCGCACTTCTGTGAATCAGTTGTTGCTATTCTTACTGTACACTCGGAGATCTGCTCAAAAGGATCGAATGGGGAAACCACTTGCTGGAAAGTGCTCTCCAGAAGTCTGGTTAGGCATTCTCATCTCTTTTATTTTGTGCTTTTTTCTGCTTCCCTTTGATTACCCAAGTAATTTTTTGGGGGTAAATAATGGTGTTAGTCTCTTTGAAAACAAAGAAACACACCCAATTGGTATTGTAAAGAATCAGTCGATTTCTGTTATCAACCAATCTACCACAGATTCATGCTCTGGTCGTTACATTTATATCCATGATGATCTTCCTGCCAAATTCAACTATGATCTGATCAACAACTGTGAGTCTCTCACTGGAAGTAACATGCCCAATATGTGTCCACACATAAAGAATTTCGGTTTTGGTCTGGAAATTAACAACTCTGAAGGGGTTTTAACCAACAGGCGATGGTTCTCCACTAACCAGTTCTCGTTAGAACTCATATTTCACAGCAAGATGAAGCACTATGAGTGCTTGACAAACAACTCCGCTCAGGCTTCAGCTATTTATATACCAATCTATCCTGGCCTTGAGGAGAGTCAACATCTGTGGGATCCTAACCTCACAGCCAGAGACTCTTTCGGTGAAGAGTTTTCCAAGTGGGTTTCGAGTAGATTCGAATGGAGGAAAATGTGGGGGAGAGACCATTTCTTTGTTTCAGGGAGGGTTGCTTTCGACTATAGGAGGAAAACAGACAACATTTCTGACTGGGGTAGTACACTCAGGGTCTCGCCAGAGTCTAACAACATGACTATGTTGACAATTGAAGGAAGCTCACGGACAAATGATATTGCTATACCATACCCCACTGGCTTTCATCCTGCAAAGGACAGTGAGGTGTTTCAGTGGCAACACAGAGTGAGGGGGCAAGAAAGGCCTTACTTGTTCACTTTTGTTGGTGGACCAAGGCCTGACCAGCCAAACTCTATCCGGGGCAAACTTTTTGATCAGTGTCAAGCCTCAAGTACTTGCAAGGTACTAAATTGCCATTCAAGTGGGATCAATTGTGAAGAAACGGTTACTGTTATGAGGGTGTTTCAGAAATCAGTTTATTGCTTGCACCCTACAGGGGATACATTCACCAGAAAATCAGCTTTCGACTCTATTCTGGCTGGTTGCATTCCTGTTTTCTTTCATCCGGCTAGCGCTTATAACCAGTATTTATGGTACTTGCCTAAGAATTACACCAAGTATTCTGTATTTATTCCAGTGAGGAAAGCACAAGATTTGAAAGACGGTAGCATTGAGAAAATCTTACTTGGAATTTCAAAGGACATGGAATTCGCCATGAGAGAGCAAGTGATAAGCCTAATTCCAAAGTTGGTGTACGGAGATCCCAGGTCAAGATTAGAGACCAAAGATGCATTTGATATTGCTGTCAACAAAATACTTGAGAGGATAGAAAATGTAAGGAAGTTGATCAGAGAGGGGAGCGATCCTAGTATCGGATTTGCAGAGCCAGATGGTAATAAGTTCCTATTTCCTGCGGAGCTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

519

Amino Acids

59.31

Weight (kDa)

8.16

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 97 - 431 2.7e-76 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1212
AccB7I CCANNNNNTGG 1 cut(s) 92
AccBSI CCGCTC 1 cut(s) 551
AciI CCGC 2 cut(s) 549, 1547
AclWI GGATC 6 cut(s) 78, 608, 621, 1067, 1388, 1490
AcsI RAATTY 4 cut(s) 335, 412, 1323, 1340
AcuI CTGAAG 2 cut(s) 465, 543
AdeI CACNNNGTG 1 cut(s) 919
AfaI GTAC 6 cut(s) 47, 778, 1027, 1038, 1229, 1388
AfeI AGCGCT 1 cut(s) 1207
AfiI CCNNNNNNNGG 3 cut(s) 92, 1129, 1505
AgsI TTSAA 6 cut(s) 215, 340, 827, 1053, 1291, 1329
AjnI CCWGG 2 cut(s) 583, 1397
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 4 cut(s) 563, 834, 1154, 1552
AluI AGCT 4 cut(s) 563, 834, 1154, 1552
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 6 cut(s) 212, 372, 627, 696, 793, 1406
AlwI GGATC 6 cut(s) 78, 608, 621, 1067, 1388, 1490
Aor51HI AGCGCT 1 cut(s) 1207
AoxI GGCC 3 cut(s) 586, 935, 968
ApoI RAATTY 4 cut(s) 335, 412, 1323, 1340
ArsI GACNNNNNNTTYG 4 cut(s) 695, 727, 1325, 1357
AspLEI GCGC 1 cut(s) 1208
AspS9I GGNCC 1 cut(s) 962
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 2 cut(s) 656, 1132
AsuII TTCGAA 1 cut(s) 678
AsuNHI GCTAGC 1 cut(s) 1202
AvaII GGWCC 1 cut(s) 962
BamHI GGATCC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 2 cut(s) 462, 1517
BciT130I CCWGG 2 cut(s) 585, 1399
BciVI GTATCC 1 cut(s) 1126
BclI TGATCA 3 cut(s) 351, 1006, 1479
BcnI CCSGG 1 cut(s) 992
BcoDI GTCTC 6 cut(s) 212, 372, 627, 696, 793, 1406
BfaI CTAG 3 cut(s) 1203, 1500, 1553
BfmI CTRYAG 2 cut(s) 739, 1125
BfoI RGCGCY 1 cut(s) 1209
BfuI GTATCC 1 cut(s) 1126
BglII AGATCT 1 cut(s) 56
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 3 cut(s) 585, 992, 1399
Bme18I GGWCC 1 cut(s) 962
BmgT120I GGNCC 1 cut(s) 962
BmiI GGNNCC 1 cut(s) 615
BmrFI CCNGG 3 cut(s) 585, 992, 1399
BmrI ACTGGG 1 cut(s) 778
BmsI GCATC 1 cut(s) 1411
BmtI GCTAGC 1 cut(s) 1206
BmuI ACTGGG 1 cut(s) 778
BplI GAGNNNNNCTC 2 cut(s) 46, 78
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 552
Bpu14I TTCGAA 1 cut(s) 678
BpuEI CTTGAG 3 cut(s) 611, 1006, 1472
BpuMI CCSGG 1 cut(s) 992
BsaI GGTCTC 3 cut(s) 696, 793, 1406
BsaJI CCNNGG 3 cut(s) 965, 991, 1397
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 1129, 1505
Bse1I ACTGG 6 cut(s) 379, 484, 773, 874, 1216, 1268
BseBI CCWGG 2 cut(s) 585, 1399
BseDI CCNNGG 3 cut(s) 965, 991, 1397
BseGI GGATG 2 cut(s) 877, 1195
BseLI CCNNNNNNNGG 3 cut(s) 92, 1129, 1505
BseMII CTCAG 2 cut(s) 566, 796
BseNI ACTGG 6 cut(s) 379, 484, 773, 874, 1216, 1268
BseRI GAGGAG 1 cut(s) 608
BshFI GGCC 3 cut(s) 588, 937, 970
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 2 cut(s) 991, 1199
BslI CCNNNNNNNGG 3 cut(s) 92, 1129, 1505
BsmAI GTCTC 6 cut(s) 212, 372, 627, 696, 793, 1406
BsmI GAATGC 2 cut(s) 123, 1179
BsnI GGCC 3 cut(s) 588, 937, 970
Bso31I GGTCTC 3 cut(s) 696, 793, 1406
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 104
Bsp1407I TGTACA 1 cut(s) 45
BspACI CCGC 2 cut(s) 549, 1547
BspANI GGCC 3 cut(s) 588, 937, 970
BspCNI CTCAG 2 cut(s) 565, 795
BspLI GGNNCC 1 cut(s) 615
BspOI GCTAGC 1 cut(s) 1206
BspPI GGATC 6 cut(s) 78, 608, 621, 1067, 1388, 1490
BspT104I TTCGAA 1 cut(s) 678
BspTNI GGTCTC 3 cut(s) 696, 793, 1406
BsrBI CCGCTC 1 cut(s) 551
BsrGI TGTACA 1 cut(s) 45
BsrI ACTGG 6 cut(s) 379, 484, 773, 874, 1216, 1268
BssECI CCNNGG 3 cut(s) 965, 991, 1397
BssT1I CCWWGG 1 cut(s) 965
Bst2UI CCWGG 2 cut(s) 585, 1399
Bst4CI ACNGT 6 cut(s) 45, 362, 893, 1078, 1084, 1298
Bst6I CTCTTC 1 cut(s) 642
BstAUI TGTACA 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 678
BstC8I GCNNGC 3 cut(s) 10, 1118, 1204
BstDEI CTNAG 3 cut(s) 552, 782, 1236
BstF5I GGATG 2 cut(s) 877, 1195
BstH2I RGCGCY 1 cut(s) 1209
BstHHI GCGC 1 cut(s) 1208
BstMAI GTCTC 6 cut(s) 212, 372, 627, 696, 793, 1406
BstMWI GCNNNNNNNGC 2 cut(s) 934, 976
BstNI CCWGG 2 cut(s) 585, 1399
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 3 cut(s) 583, 990, 1397
BstSFI CTRYAG 2 cut(s) 739, 1125
BstX2I RGATCY 3 cut(s) 56, 613, 1393
BstXI CCANNNNNNTGG 2 cut(s) 114, 1055
BstYI RGATCY 3 cut(s) 56, 613, 1393
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 3 cut(s) 588, 937, 970
BtgZI GCGATG 1 cut(s) 481
BtsCI GGATG 2 cut(s) 877, 1195
BtsIMutI CAGTG 6 cut(s) 372, 867, 898, 911, 1016, 1275
Cac8I GCNNGC 3 cut(s) 10, 1118, 1204
CfoI GCGC 1 cut(s) 1208
Cfr13I GGNCC 1 cut(s) 962
Csp6I GTAC 6 cut(s) 46, 777, 1026, 1037, 1228, 1387
CspCI CAANNNNNGTGG 2 cut(s) 590, 625
CviAII CATG 6 cut(s) 291, 317, 385, 808, 1336, 1348
CviQI GTAC 6 cut(s) 46, 777, 1026, 1037, 1228, 1387
DdeI CTNAG 3 cut(s) 552, 782, 1236
DraIII CACNNNGTG 1 cut(s) 919
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 965
Eco147I AGGCCT 2 cut(s) 937, 970
Eco31I GGTCTC 3 cut(s) 696, 793, 1406
Eco47I GGWCC 1 cut(s) 962
Eco47III AGCGCT 1 cut(s) 1207
Eco57I CTGAAG 2 cut(s) 465, 543
EcoRI GAATTC 1 cut(s) 1340
EcoRII CCWGG 2 cut(s) 583, 1397
EcoT14I CCWWGG 1 cut(s) 965
EcoT22I ATGCAT 1 cut(s) 1426
ErhI CCWWGG 1 cut(s) 965
FaeI CATG 6 cut(s) 294, 320, 388, 811, 1339, 1351
FatI CATG 6 cut(s) 290, 316, 384, 807, 1335, 1347
FbaI TGATCA 3 cut(s) 351, 1006, 1479
FokI GGATG 2 cut(s) 864, 1182
FspBI CTAG 3 cut(s) 1203, 1500, 1553
GlaI GCGC 1 cut(s) 1207
GsuI CTGGAG 1 cut(s) 90
HaeII RGCGCY 1 cut(s) 1209
HaeIII GGCC 3 cut(s) 588, 937, 970
HapII CCGG 2 cut(s) 991, 1199
HhaI GCGC 1 cut(s) 1208
Hin1II CATG 6 cut(s) 294, 320, 388, 811, 1339, 1351
Hin6I GCGC 1 cut(s) 1206
HinP1I GCGC 1 cut(s) 1206
HincII GTYRAC 3 cut(s) 602, 819, 1441
HindII GTYRAC 3 cut(s) 602, 819, 1441
HinfI GANTC 9 cut(s) 22, 250, 287, 365, 598, 635, 675, 797, 1160
HpaII CCGG 2 cut(s) 991, 1199
HphI GGTGA 2 cut(s) 656, 1132
Hpy166II GTNNAC 9 cut(s) 48, 401, 602, 779, 819, 948, 962, 1387, 1441
Hpy188I TCNGA 7 cut(s) 54, 351, 445, 766, 1101, 1484, 1508
Hpy188III TCNNGA 3 cut(s) 107, 428, 1404
Hpy8I GTNNAC 9 cut(s) 48, 401, 602, 779, 819, 948, 962, 1387, 1441
HpyAV CCTTC 2 cut(s) 440, 821
HpyCH4III ACNGT 6 cut(s) 45, 362, 893, 1078, 1084, 1298
HpyCH4V TGCA 6 cut(s) 884, 1032, 1120, 1179, 1424, 1514
HpyF10VI GCNNNNNNNGC 2 cut(s) 934, 976
HpyF3I CTNAG 3 cut(s) 552, 782, 1236
Hsp92II CATG 6 cut(s) 294, 320, 388, 811, 1339, 1351
HspAI GCGC 1 cut(s) 1206
Ksp22I TGATCA 3 cut(s) 351, 1006, 1479
LmnI GCTCC 2 cut(s) 1491, 1549
LweI GCATC 1 cut(s) 1411
MaeI CTAG 3 cut(s) 1203, 1500, 1553
MaeIII GTNAC 3 cut(s) 302, 380, 1078
MbiI CCGCTC 1 cut(s) 551
MboII GAAGA 3 cut(s) 317, 659, 1082
MfeI CAATTG 3 cut(s) 234, 822, 1063
MflI RGATCY 3 cut(s) 56, 613, 1393
MhlI GDGCHC 1 cut(s) 104
MlyI GAGTC 5 cut(s) 374, 607, 629, 806, 1154
Mph1103I ATGCAT 1 cut(s) 1426
MseI TTAA 2 cut(s) 435, 455
MslI CAYNNNNRTG 3 cut(s) 17, 812, 1053
MspI CCGG 2 cut(s) 991, 1199
MspR9I CCNGG 3 cut(s) 585, 992, 1399
MunI CAATTG 3 cut(s) 234, 822, 1063
Mva1269I GAATGC 2 cut(s) 123, 1179
MvaI CCWGG 2 cut(s) 585, 1399
MwoI GCNNNNNNNGC 2 cut(s) 934, 976
NciI CCSGG 1 cut(s) 992
NheI GCTAGC 1 cut(s) 1202
NlaIII CATG 6 cut(s) 294, 320, 388, 811, 1339, 1351
NlaIV GGNNCC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 1426
NspI RCATGY 1 cut(s) 388
NspV TTCGAA 1 cut(s) 678
OliI CACNNNNGTG 1 cut(s) 17
PceI AGGCCT 2 cut(s) 937, 970
PctI GAATGC 2 cut(s) 123, 1179
PfeI GAWTC 4 cut(s) 22, 250, 287, 675
PflMI CCANNNNNTGG 1 cut(s) 92
PleI GAGTC 5 cut(s) 373, 606, 629, 805, 1154
PpsI GAGTC 5 cut(s) 373, 606, 629, 805, 1154
PsiI TTATAA 1 cut(s) 1212
Psp6I CCWGG 2 cut(s) 583, 1397
PspGI CCWGG 2 cut(s) 583, 1397
PspN4I GGNNCC 1 cut(s) 615
PspPI GGNCC 1 cut(s) 962
PsuI RGATCY 3 cut(s) 56, 613, 1393
RsaI GTAC 6 cut(s) 47, 778, 1027, 1038, 1229, 1388
RsaNI GTAC 6 cut(s) 46, 777, 1026, 1037, 1228, 1387
RseI CAYNNNNRTG 3 cut(s) 17, 812, 1053
SaqAI TTAA 2 cut(s) 435, 455
Sau96I GGNCC 1 cut(s) 962
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 5 cut(s) 374, 607, 629, 806, 1154
ScrFI CCNGG 3 cut(s) 585, 992, 1399
SduI GDGCHC 1 cut(s) 104
SetI ASST 8 cut(s) 565, 624, 836, 900, 1038, 1156, 1403, 1554
SfaNI GCATC 1 cut(s) 1411
SfcI CTRYAG 2 cut(s) 739, 1125
SfuI TTCGAA 1 cut(s) 678
SinI GGWCC 1 cut(s) 962
SmiMI CAYNNNNRTG 3 cut(s) 17, 812, 1053
SmlI CTYRAG 3 cut(s) 590, 1021, 1451
SmoI CTYRAG 3 cut(s) 590, 1021, 1451
SseBI AGGCCT 2 cut(s) 937, 970
SsiI CCGC 2 cut(s) 549, 1547
SspMI CTAG 3 cut(s) 1203, 1500, 1553
StuI AGGCCT 2 cut(s) 937, 970
StyD4I CCNGG 3 cut(s) 583, 990, 1397
StyI CCWWGG 1 cut(s) 965
TaaI ACNGT 6 cut(s) 45, 362, 893, 1078, 1084, 1298
TaqI TCGA 6 cut(s) 73, 257, 668, 678, 735, 1158
TatI WGTACW 3 cut(s) 45, 776, 1025
TfiI GAWTC 4 cut(s) 22, 250, 287, 675
Tru1I TTAA 2 cut(s) 435, 455
Tru9I TTAA 2 cut(s) 435, 455
TscAI CASTG 6 cut(s) 379, 874, 898, 911, 1016, 1275
TspDTI ATGAA 4 cut(s) 279, 533, 866, 1184
TspGWI ACGGA 2 cut(s) 853, 1404
TspRI CASTG 6 cut(s) 379, 874, 898, 911, 1016, 1275
Van91I CCANNNNNTGG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 962
XapI RAATTY 4 cut(s) 335, 412, 1323, 1340
XceI RCATGY 1 cut(s) 388
XspI CTAG 3 cut(s) 1203, 1500, 1553
ZrmI AGTACT 1 cut(s) 1027
Zsp2I ATGCAT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.