Rh4CG190400

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000894
Physical Location & Seq
Reverse (-)
41022840 .. 41025581
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG190400.1

Sequence Viewer

Length: 1503 bp
ATGTTCAAGTCCTTCTGGTTTGAGAGAGCTGCTCATCCAGACCAAATGGAGAAACTACTTACCGGAAAGTGTTCTCGAGAAGTCTTGTTTGGCATACTCGTGTCCTTTTGTTTATGCTTTGCATTGCTTTGCCTGCACAATTCAACTTCATTGGGTTTCAGCAATTGGGTTAACATTTTGGGGAACAGTCATGTTCACCACATATTTGATTCTTGCTCCGGTCGTTACATTTATGTCTATGATGATCTTCCTGCCAAATTCAACTATGACCTGCTCAGAAACTGTAAGTCACTTACTTCAGGGAGTACTAGTGATTCTGGTGACGAAAAAAATATGTGTCCTTACCTTGTGAATATGGGTCTTGGTCCTGAAATTGAGAGCTCTAAAGGTGTTTTAGCAAACAAGAGTTGGTTTACCACAAACCCCTACTTGTTAGAAGTCATATTCCACAACAGGATGAAGCAGTACGAGTGCTTGACAAACATCTCCACTCTGGCTTCAGCAATTTATGTACCATACTACACTGGCCTTGACGCCAGTCTCCATCTTTGGGATACTAACCTCACCGTCAGAGACTCTTCGGGTAAAGATCTAGTCAAGTGGCTTTCAGGGAAACCTGAATGGAAGACAATGTGGGGCAGAGACCATTTCATGGTGACTGGCAGGACTTCTTGGGATTTCAGGAGGACTAACGACAATGCGGAATGGCCATGGGGCAGTGGTCTCAGGTTCTTGCCAGAGTCAAAGAACATGAGTATGCTTTCGGTAGAAGGAAGCTTGTGGAAAAATGACTTTGCTATTCCATACCCCACGAACTTTCATCCAGCAAAGGATATTGAGATTATCCAGTGGCAGAACAGAATGAGATATCTAGAGAGGCCTTACTTGTTTTCTTTTATTGGAGCGCCAAGGCCTGGCTTGGAGAAATCTATCAGGGGGGAACTTATTGATCAGTGCCGAGCTTCAAGTACTTGCAAGTTTGTAAATTGTGGTGCTGATGGGATCAATTGTAACAACCCGATTACTGTTATGAAGGTGTTTCAAAGCTCAGTTTATTGCTTGCAGCCTCCAGGGGATTCACACACTAGGAGATCAGCTTTCGACTCTATGCTGGCTGGTTGTATTCCTGTTTTCTTCCATCCAGGTTCAGCTTATAACCAATATTTATGGCATTTACCAAAGAATCATAGCAGATATTCGGTGTTTATCCCCGCAAAGAATGTGACTGATTTGAAAGAAGTCTCTATTGAGAAACTTTTGCTTGGAATTTCAAAGGAGAAGCAATTAGCAATGAGAAAGAAGGTTATAAAGCTAATACCAAAGCTGGTATATGCAGATCCCAGGTCAAGACTGGGGACTGAAGACTCATTTGATTTTGCTGTTATGGGAATTCTTGAGAGAATAGAGAATGTTAGGCAGTTGATTAGAGAGGGAAAAGATCCTAGTATTGGTTTTGCAGATGAAGATAGTTTAAAATACACATTTCCTGAGACACTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

500

Amino Acids

57.16

Weight (kDa)

8.27

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 71 - 414 4.7e-80 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1155, 1307
Acc36I ACCTGC 1 cut(s) 279
AccB7I CCANNNNNTGG 2 cut(s) 652, 914
AciI CCGC 2 cut(s) 701, 1212
AclWI GGATC 3 cut(s) 1010, 1331, 1433
AcoI YGGCCR 1 cut(s) 707
AcsI RAATTY 3 cut(s) 257, 1266, 1389
AcuI CTGAAG 3 cut(s) 282, 483, 1380
AcyI GRCGYC 1 cut(s) 534
AfaI GTAC 4 cut(s) 307, 467, 513, 970
AfiI CCNNNNNNNGG 4 cut(s) 550, 652, 914, 1448
AgsI TTSAA 7 cut(s) 7, 144, 262, 966, 1043, 1234, 1272
AhlI ACTAGT 1 cut(s) 308
AjnI CCWGG 4 cut(s) 913, 1069, 1141, 1340
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 9 cut(s) 29, 381, 777, 962, 1047, 1097, 1151, 1312, 1324
AluI AGCT 9 cut(s) 29, 381, 777, 962, 1047, 1097, 1151, 1312, 1324
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 6 cut(s) 545, 567, 636, 728, 1246, 1484
AlwI GGATC 3 cut(s) 1010, 1331, 1433
AlwNI CAGNNNCTG 1 cut(s) 282
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 4 cut(s) 526, 707, 878, 911
ApeKI GCWGC 2 cut(s) 29, 1063
ApoI RAATTY 3 cut(s) 257, 1266, 1389
Asp700I GAANNNNTTC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 4 cut(s) 188, 332, 556, 667
AvaI CYCGRG 1 cut(s) 75
AvaII GGWCC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 709
BanII GRGCYC 1 cut(s) 383
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 632, 1368
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 2 cut(s) 16, 1075
BccI CCATC 3 cut(s) 552, 992, 1146
BciT130I CCWGG 4 cut(s) 915, 1071, 1143, 1342
BciVI GTATCC 1 cut(s) 547
BclI TGATCA 1 cut(s) 949
BcoDI GTCTC 6 cut(s) 545, 567, 636, 728, 1246, 1484
BcuI ACTAGT 1 cut(s) 308
BfaI CTAG 6 cut(s) 309, 593, 872, 1086, 1443, 1496
BfoI RGCGCY 1 cut(s) 908
BfuAI ACCTGC 1 cut(s) 279
BfuI GTATCC 1 cut(s) 547
BglII AGATCT 1 cut(s) 589
BisI GCNGC 2 cut(s) 30, 1064
BlsI GCNGC 2 cut(s) 31, 1065
BmcAI AGTACT 2 cut(s) 307, 970
Bme1390I CCNGG 4 cut(s) 915, 1071, 1143, 1342
Bme18I GGWCC 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 365
BmrFI CCNGG 4 cut(s) 915, 1071, 1143, 1342
BmrI ACTGGG 1 cut(s) 1361
BmuI ACTGGG 1 cut(s) 1361
BoxI GACNNNNGTC 1 cut(s) 537
BpiI GAAGAC 2 cut(s) 632, 1368
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpmI CTGGAG 1 cut(s) 1053
BpuEI CTTGAG 1 cut(s) 1415
BsaHI GRCGYC 1 cut(s) 534
BsaI GGTCTC 2 cut(s) 636, 728
BsaJI CCNNGG 4 cut(s) 710, 908, 1070, 1340
BsaWI WCCGGW 2 cut(s) 62, 218
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bsc4I CCNNNNNNNGG 4 cut(s) 550, 652, 914, 1448
Bse1I ACTGG 5 cut(s) 529, 537, 664, 847, 1356
Bse3DI GCAATG 2 cut(s) 122, 1296
BseBI CCWGG 4 cut(s) 915, 1071, 1143, 1342
BseDI CCNNGG 4 cut(s) 710, 908, 1070, 1340
BseGI GGATG 4 cut(s) 34, 462, 820, 1138
BseLI CCNNNNNNNGG 4 cut(s) 550, 652, 914, 1448
BseMI GCAATG 2 cut(s) 122, 1296
BseMII CTCAG 4 cut(s) 289, 739, 1062, 1479
BseNI ACTGG 5 cut(s) 529, 537, 664, 847, 1356
BseXI GCAGC 2 cut(s) 16, 1075
BsgI GTGCAG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 4 cut(s) 528, 709, 880, 913
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 2 cut(s) 63, 219
BslFI GGGAC 1 cut(s) 1369
BslI CCNNNNNNNGG 4 cut(s) 550, 652, 914, 1448
BsmAI GTCTC 6 cut(s) 545, 567, 636, 728, 1246, 1484
BsmFI GGGAC 1 cut(s) 1369
BsnI GGCC 4 cut(s) 528, 709, 880, 913
Bso31I GGTCTC 2 cut(s) 636, 728
BsoBI CYCGRG 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 2 cut(s) 701, 1212
BspANI GGCC 4 cut(s) 528, 709, 880, 913
BspCNI CTCAG 4 cut(s) 288, 738, 1061, 1480
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 3 cut(s) 1010, 1331, 1433
BspTNI GGTCTC 2 cut(s) 636, 728
BsrDI GCAATG 2 cut(s) 122, 1296
BsrI ACTGG 5 cut(s) 529, 537, 664, 847, 1356
BssECI CCNNGG 4 cut(s) 710, 908, 1070, 1340
BssMI GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
BssNI GRCGYC 1 cut(s) 534
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 2 cut(s) 710, 908
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 4 cut(s) 915, 1071, 1143, 1342
Bst4CI ACNGT 4 cut(s) 188, 284, 568, 1027
Bst6I CTCTTC 1 cut(s) 583
BstACI GRCGYC 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 134, 1061, 1113
BstDEI CTNAG 4 cut(s) 275, 725, 1048, 1488
BstDSI CCRYGG 1 cut(s) 710
BstF5I GGATG 4 cut(s) 34, 462, 820, 1138
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 7 cut(s) 247, 592, 952, 1005, 1094, 1339, 1441
BstMAI GTCTC 6 cut(s) 545, 567, 636, 728, 1246, 1484
BstMBI GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 4 cut(s) 915, 1071, 1143, 1342
BstPAI GACNNNNGTC 1 cut(s) 537
BstSCI CCNGG 4 cut(s) 913, 1069, 1141, 1340
BstV1I GCAGC 2 cut(s) 16, 1075
BstV2I GAAGAC 2 cut(s) 632, 1368
BstX2I RGATCY 3 cut(s) 589, 1336, 1438
BstYI RGATCY 3 cut(s) 589, 1336, 1438
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 4 cut(s) 528, 709, 880, 913
BtgI CCRYGG 1 cut(s) 710
BtsCI GGATG 4 cut(s) 34, 462, 820, 1138
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 4 cut(s) 522, 724, 854, 959
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 3 cut(s) 134, 1061, 1113
CaiI CAGNNNCTG 1 cut(s) 282
CfoI GCGC 1 cut(s) 907
Cfr13I GGNCC 1 cut(s) 365
CseI GACGC 1 cut(s) 542
Csp6I GTAC 4 cut(s) 306, 466, 512, 969
CviAII CATG 4 cut(s) 191, 652, 711, 751
CviQI GTAC 4 cut(s) 306, 466, 512, 969
DdeI CTNAG 4 cut(s) 275, 725, 1048, 1488
DpnI GATC 7 cut(s) 246, 591, 951, 1004, 1093, 1338, 1440
DpnII GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
DraI TTTAAA 1 cut(s) 1473
EaeI YGGCCR 1 cut(s) 707
Eam1104I CTCTTC 1 cut(s) 583
EarI CTCTTC 1 cut(s) 583
Ecl136II GAGCTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 710, 908
Eco147I AGGCCT 2 cut(s) 880, 913
Eco24I GRGCYC 1 cut(s) 383
Eco31I GGTCTC 2 cut(s) 636, 728
Eco32I GATATC 1 cut(s) 869
Eco47I GGWCC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 381
Eco57I CTGAAG 3 cut(s) 282, 483, 1380
Eco88I CYCGRG 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 1389
EcoRII CCWGG 4 cut(s) 913, 1069, 1141, 1340
EcoRV GATATC 1 cut(s) 869
EcoT14I CCWWGG 2 cut(s) 710, 908
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 2 cut(s) 710, 908
FaeI CATG 4 cut(s) 194, 655, 714, 754
FaqI GGGAC 1 cut(s) 1369
FatI CATG 4 cut(s) 190, 651, 710, 750
FauI CCCGC 1 cut(s) 1219
FbaI TGATCA 1 cut(s) 949
Fnu4HI GCNGC 2 cut(s) 30, 1064
FokI GGATG 4 cut(s) 21, 469, 807, 1125
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 2 cut(s) 30, 1064
FspBI CTAG 6 cut(s) 309, 593, 872, 1086, 1443, 1496
GlaI GCGC 1 cut(s) 906
GluI GCNGC 2 cut(s) 30, 1064
GsuI CTGGAG 1 cut(s) 1053
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 4 cut(s) 528, 709, 880, 913
HapII CCGG 2 cut(s) 63, 219
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 907
Hin1I GRCGYC 1 cut(s) 534
Hin1II CATG 4 cut(s) 194, 655, 714, 754
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 775
HinfI GANTC 8 cut(s) 209, 314, 575, 740, 1076, 1103, 1183, 1364
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 2 cut(s) 63, 219
HphI GGTGA 4 cut(s) 188, 332, 556, 667
Hpy166II GTNNAC 3 cut(s) 172, 196, 414
Hpy188I TCNGA 2 cut(s) 278, 572
Hpy188III TCNNGA 9 cut(s) 38, 75, 77, 368, 682, 872, 1347, 1394, 1487
Hpy8I GTNNAC 3 cut(s) 172, 196, 414
HpyAV CCTTC 4 cut(s) 22, 764, 1027, 1294
HpyCH4III ACNGT 4 cut(s) 188, 284, 568, 1027
HpyCH4V TGCA 6 cut(s) 122, 136, 975, 1063, 1334, 1457
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 4 cut(s) 275, 725, 1048, 1488
Hsp92I GRCGYC 1 cut(s) 534
Hsp92II CATG 4 cut(s) 194, 655, 714, 754
HspAI GCGC 1 cut(s) 905
Ksp22I TGATCA 1 cut(s) 949
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
LmnI GCTCC 2 cut(s) 221, 902
Lsp1109I GCAGC 2 cut(s) 16, 1075
MaeI CTAG 6 cut(s) 309, 593, 872, 1086, 1443, 1496
MaeIII GTNAC 6 cut(s) 224, 288, 320, 655, 1010, 1222
MalI GATC 7 cut(s) 246, 591, 951, 1004, 1093, 1338, 1440
MboI GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
MboII GAAGA 6 cut(s) 239, 570, 637, 1126, 1373, 1475
MfeI CAATTG 2 cut(s) 163, 1006
MflI RGATCY 3 cut(s) 589, 1336, 1438
MhlI GDGCHC 1 cut(s) 383
MlsI TGGCCA 1 cut(s) 709
MluNI TGGCCA 1 cut(s) 709
MlyI GAGTC 4 cut(s) 569, 749, 1097, 1358
MnlI CCTC 5 cut(s) 572, 678, 870, 1077, 1423
Mox20I TGGCCA 1 cut(s) 709
MroXI GAANNNNTTC 1 cut(s) 70
MscI TGGCCA 1 cut(s) 709
MseI TTAA 2 cut(s) 171, 1472
MslI CAYNNNNRTG 2 cut(s) 98, 755
Msp20I TGGCCA 1 cut(s) 709
MspI CCGG 2 cut(s) 63, 219
MspR9I CCNGG 4 cut(s) 915, 1071, 1143, 1342
MunI CAATTG 2 cut(s) 163, 1006
MvaI CCWGG 4 cut(s) 915, 1071, 1143, 1342
MwoI GCNNNNNNNGC 1 cut(s) 133
NcoI CCATGG 1 cut(s) 710
NdeII GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
NlaIII CATG 4 cut(s) 194, 655, 714, 754
NmeAIII GCCGAG 1 cut(s) 983
NmuCI GTSAC 4 cut(s) 288, 320, 655, 1222
PaeR7I CTCGAG 1 cut(s) 75
PceI AGGCCT 2 cut(s) 880, 913
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 4 cut(s) 209, 314, 1076, 1183
PflMI CCANNNNNTGG 2 cut(s) 652, 914
PkrI GCNGC 2 cut(s) 31, 1065
PleI GAGTC 4 cut(s) 569, 748, 1097, 1358
PpsI GAGTC 4 cut(s) 569, 748, 1097, 1358
PshAI GACNNNNGTC 1 cut(s) 537
PsiI TTATAA 2 cut(s) 1155, 1307
Psp124BI GAGCTC 1 cut(s) 383
Psp6I CCWGG 4 cut(s) 913, 1069, 1141, 1340
PspGI CCWGG 4 cut(s) 913, 1069, 1141, 1340
PspPI GGNCC 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 282
PsuI RGATCY 3 cut(s) 589, 1336, 1438
RsaI GTAC 4 cut(s) 307, 467, 513, 970
RsaNI GTAC 4 cut(s) 306, 466, 512, 969
RseI CAYNNNNRTG 2 cut(s) 98, 755
SacI GAGCTC 1 cut(s) 383
SaqAI TTAA 2 cut(s) 171, 1472
SatI GCNGC 2 cut(s) 30, 1064
Sau3AI GATC 7 cut(s) 244, 589, 949, 1002, 1091, 1336, 1438
Sau96I GGNCC 1 cut(s) 365
ScaI AGTACT 2 cut(s) 307, 970
SchI GAGTC 4 cut(s) 569, 749, 1097, 1358
ScrFI CCNGG 4 cut(s) 915, 1071, 1143, 1342
SduI GDGCHC 1 cut(s) 383
Sfr274I CTCGAG 1 cut(s) 75
SinI GGWCC 1 cut(s) 365
SlaI CTCGAG 1 cut(s) 75
SmiMI CAYNNNNRTG 2 cut(s) 98, 755
SmlI CTYRAG 2 cut(s) 75, 1394
SmoI CTYRAG 2 cut(s) 75, 1394
SpeI ACTAGT 1 cut(s) 308
SseBI AGGCCT 2 cut(s) 880, 913
SsiI CCGC 2 cut(s) 701, 1212
SspI AATATT 1 cut(s) 1163
SspMI CTAG 6 cut(s) 309, 593, 872, 1086, 1443, 1496
SstI GAGCTC 1 cut(s) 383
StuI AGGCCT 2 cut(s) 880, 913
StyD4I CCNGG 4 cut(s) 913, 1069, 1141, 1340
StyI CCWWGG 2 cut(s) 710, 908
TaaI ACNGT 4 cut(s) 188, 284, 568, 1027
TaqI TCGA 2 cut(s) 76, 1101
TatI WGTACW 2 cut(s) 305, 968
TfiI GAWTC 4 cut(s) 209, 314, 1076, 1183
Tru1I TTAA 2 cut(s) 171, 1472
Tru9I TTAA 2 cut(s) 171, 1472
TscAI CASTG 4 cut(s) 529, 724, 854, 959
TseFI GTSAC 4 cut(s) 288, 320, 655, 1222
TseI GCWGC 2 cut(s) 29, 1063
Tsp45I GTSAC 4 cut(s) 288, 320, 655, 1222
TspDTI ATGAA 6 cut(s) 138, 473, 640, 809, 1046, 1476
TspRI CASTG 4 cut(s) 529, 724, 854, 959
Van91I CCANNNNNTGG 2 cut(s) 652, 914
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 3 cut(s) 257, 1266, 1389
XbaI TCTAGA 1 cut(s) 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 1348
XhoI CTCGAG 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 6 cut(s) 309, 593, 872, 1086, 1443, 1496
ZrmI AGTACT 2 cut(s) 307, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.