FvH4_5g20730

SNF2 domain-containing protein CLASSY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001072
Physical Location & Seq
Reverse (-)
12302928 .. 12311829
8902 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20730.t1

Sequence Viewer

Length: 4374 bp
ATGGATTATAGACCTGTATCAACAAGAACAAGGGCTAAGAGAGACGAAGCTCTGTTGGAAAGATATTGGGAGCTGAAGGCTAACAGTGGGGAAATTCCAGCAAGGAGTAAGAAAATGCGAAGGTCCAACCAGCAGGCTTCCCATTCTCATGTTGGTGAGTCCAGCTCTAGGAAACAAAATTTTGAGTGGTTAAGTGATGATGTGGAATCCCTCGAAAACCATGATAATGTTTCAAAGAGCTTTGATTCTCGAGAGAGTGTTATAAAGAAAAGTGGGGGAAAGGGAAAGGGGAAAGGAGCTGCGGTTGGCTTGAAGAGAAAGAGAAGTGATGATAGCAAAAATGATGGTTGTAAACATTGGAGGGTTGTTTCAGATGATGGGGTTGAGGTTGTCGATTTAGTGGATAGTGATGATGATGATGATGATGAGGTAGTAGTTTTGAAAGGTAAAGAGGGAGGAGGAGGAGTTGATGATGTGAATGATGGTAGAAATTATGATGATGTTGTTGATGTAAGTGATGATGATGATACTGATGATGATGAGGAGGAGGAGGTTTCATCAGCAGAGGGAGGCAACAGTAGTGATGAAAGTGAGTTCTTGACAGAGGAAGATTCGACAGATGAAGACTATGATGAGGGAAGTGTTTCCTCATCAGATGAAGACAGTAGTGAGGAGGAAAAGAAAGAGGAGACTGAGATAACAGCAGAGAAGGATGAGAAAGAGTGCAAAGAAGAAAGTGTGGAGATTTTCTTAAAGAGAAAGAGCAGTGCTAATGTGGATGACCTGCAATCTTCTTGTGGTCAGGATATTAATAATGAAAGAAGTGAGACAGTGAGCAAAGTGGAAACTGCGAAGCTTGTTCCGGAAGGAATGATCACTGATGATGGTGATGATCTGCATTCTTGCTCTGCTCAGAATGCTGATGTCAGAAGTGAGCAAGTGCAGAAGGAAGAAAGTCTCGGGATTTTTCCTAAAGGAAATGCTAATGCTGATGGGGATGATCTACATTCTTCTTTTGGTCATGTTATCATTGACAGAAGTGAGAAACTGGTGCAGGCAGAGAGTACTTTCTTGGCTGAAGAGAGAACTGGGTGTGATAAAAGTGATGATTGCAAGGATGGGAGAGTGTGGAGGAAAGAAGAAAGTGTTGTGATTGGTTCGAATGAAAACAGAAGTGGTGATGTGGGTGATCAATGCAAACACTCTGAAGGGGACAGAAGTGTTGATTACAAACAAGGGAGAGTGTGGAAAAAAGAAGAAAGTGTTGCGGTTGGTTCGAAGGAAAACAGAAGTGGTGATGTGGGTGATCAATGCAAACAGTCTGAAGGGGACAGAAGTGTTGATTACAAACATGGGAGAGTGTGGAAGAAAGACGAAAGTGTTGGGGTTAGTGGTGATTACAAATATGGGAGAGTGTGGAAGAAAGAAGAAGCTGTTGGGGTTAGCTTGAAGGAGAAGGGAAACAGAAGTGTTGATGTGCATAACCAATGCAAAGTTTCTGAAGGGGACAGCTCCCTTACCAAAAAAACCAGCAAAAGGGCACCAAAACAGAAAAGGGTACCTACTATTGCATTTCAACCCCCAAAAGTTGAATTTCTTCCTCAGAGATTCAGTTTTGATAAGAAGCCAACTGTTCCTGAGAAATCAGACTTTGAGAGAGAAGTTGAGAACCTTTTCGATGAATTTTCAACTGCCAAGGCATATGCCAATGCTTCTACTGATTCTAAGGCTAAACAAAACAACTGTACAAGATTTGATGATAACAGAAACGATGTCATTGATGAGACTTATGGTGGTAGTTCAGAAATGGCAATTGATAATAGGAGAATAATTGATATGCAGACGGCATTCAACGAGAATGAAGCTGCCAAAGGAAACAACTGTTCAAGGTCCACTAATACACTAAAGAATGACAATGGTGAGACATATGGTGGTAGTCATCCTGAACCATATAAAAAGAAAGAAGAGGTGAAGAAGCTGAGCACCAAAAAGGGCATTACAGCACCAAAAGATTGCAATGCCTTTACGATTCTTTTAGATTCAATTTATGACAAGGGAGAAGTATCAGGGGAAAAGTTACCTCCTTTTGGGGATGAATCTCCCAAAGATGAAGCTCTAAGATATGAAGCTCCACGAGATGAAGCTCCAATAGTTGAACCTCTTCCGTTGTTTTTCACATTTGGTCATCAGCCAAGTCCTCCACCAGAGAAGTCAGCTCACGAGATAGAACTAGATAATCTTTTTGATGAACTCAAATTTGCCCGCTTGTGTAACGAGATTGGTTCCACTGATTTTAATGTGGTTGAAAGTAAGCACTCAGCCCCTCCCGGTAAGGAACTTACTCAGCATGAACTCTGTGGTCAAGGGAAGCACGAACTCGAACTTGATGAAGAAATTGGACTTATATGTCGATATTGCCCTCATGTGCATCAGGAGATCAAGTATATTGTACCAGACTTTGCTCCAAACCCTTATGGAAAATATGAGAAGAGGTTCCATGAAGAAGATAATCGGTCTATCCTAGAAGATCTTCAGCTTAACGACTGCAACGATTCTGGCTTTAGCTCCCATGCTCATACTGAAGGCACTGTGTGGGACTTAATCCCTGGCGTTGAAAGTGATATGTATCCTCATCAGCGTGAAGGTTTTGAATTTATTTGGAGTCACTTAGCTGGTGGAATTGAGCTCGGAAAGTTGGCACAACCTAGTGCTTATGATGGTGATGGATGCATTATATCACATGCACCTGGAACTGGGAAGACTCGTCTGACTATCATCTTCATCCAGTCATACATGAATTTCAACCCATCAAGCAGGCCACTCATAGTTGCTCCACGTACCATGCTGCTTACTTGGGAAGAGGAGTTTAAGAAATGGGGTTTCGACATTCCCTTTCACAATCTTAACAATCTTGAGTTCACTGGGAAAGAAGATGCAGCAGCTCACAATCTTGTGAAGGATGCTAAAAGGTCAGAACTGACAAACTTGACCCGTGTAGTTAAGCTAAAGTCGTGGGCAAAGACAAGAAGCATCTTGGGGATTAGTTATTCTCTGTTTGAGCAGATATGTGGAGCTTCTGTCAAGAAAGGAAAACTTGAACAACTTTCTGCAAGACGTGTAGAAGAAGCTGAGCAGATGAGTAAAATCCTTCTTGAATTTCCTGGTCTTGTAGTATTTGATGAAGGGCACAATCCTCGCAATGACCAGAGTTACTCATGGAAGTCTCTCTTGAGACTAAAAACAAGAAGGCGCATTATCCTCTCTGGAACTCCTTTCCAGAATAATTTTGAGGAGCTCTACAATACAGTTCGCTTGGTGAGGCCTGAATGGACCACCAATCTTACCTGCCTTGGAGGTTTCGACAAGAAGCAATGGGCTTCATACCAAGTGAGAGGCACAAAGGGGAATGCAAGGAAAAGCCAATTTGCTGATGATAATACAAAGCGTGCCAGAGAGGTCAGAGAGATGATTTCACCATTTGTGCATGTTTACAAAGGCTCCATACTGCAAGATAAGCTTCCCGGGCTAAGGAACTCGGTTGTTGTTTTACAGCCGACAGCCTTACAGCAGCGATATTCAAACAGAACTCGTATGATAAAAAATCCATTCAAATATGAAAGAATAGAGGCTAAAGTGACCATCCATCCATCCCTCTTGCTTAGTGAAGAAAGGTCTACGTTTTTTGAGGAAGATGAGTATGCAGCTGACTGGGAAAGCCTAAAGGAAATTAAGTTGAATCCTGAAGCTGGTGTGAAGGCAAGATTTACTGTTGATCTCATTCGGCTTTGTCAAGCTATGAATGAAAGAGTTTTGGTGTTCAGTCAGTGGGTTGATCCATTGCACTTGATGAGGGAGCTGCTCAGACGTCAGTTTCATTGGGTGGAAGGAGAAGAGGTAATATATATGGATGGAAAATGTGATGCAAAGCAACGCCAATCCTCGATGAGTTATTTCAATGATCCATCCACCAAAGCAAAGGTTATGTTAGCTACAACCAAAGGCTGTAATGAAGGAATCAGTTTAGTTGGAGCTTCAAGGGTTGTACTGCTTGACGTTACATGGAACCCGTCTGTCGAGAGGCAAGCAATCTCCCGGGCATATCGTCTGGGACAGAAGAAAATTGTGTCCATCTACCATCTCCTCATGGCGGGAACAAGAGAAGAGGACAAATACACCCGGCAAGTTGAGAAGCATCGGTTGTCTGAATTGGTGTTTTCTTGTTCGGATACAGAAGCTGCCAAGAAGATCCCACCTGCAGTTGCTGAGGACAAGATTTTGGAAGAGATGGTTCAGCACGAGAAACTTAAGCATATATTTATGAAGATAACATTACTGGATGAAGCCTCATATTTGGATGAAGATTATGAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1458

Amino Acids

164.79

Weight (kDa)

5.18

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 875 - 1092 1.8e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 878 - 1201 9.1e-26 SNF2-related domain
Helicase_C PF00271 1258 - 1370 7.9e-17 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
AarI CACCTGC 1 cut(s) 4261
AasI GACNNNNNNGTC 1 cut(s) 2406
AatII GACGTC 1 cut(s) 3868
Acc36I ACCTGC 3 cut(s) 792, 3353, 4261
Acc65I GGTACC 1 cut(s) 1558
AccB1I GGYRCC 2 cut(s) 1540, 1558
AccI GTMKAC 1 cut(s) 3674
AccIII TCCGGA 1 cut(s) 862
AciI CCGC 4 cut(s) 302, 1268, 2263, 4148
AclWI GGATC 3 cut(s) 3827, 3953, 4240
AcsI RAATTY 9 cut(s) 93, 178, 1592, 1682, 2255, 2651, 2797, 3155, 4367
AcuI CTGAAG 8 cut(s) 95, 1098, 1227, 1344, 1521, 2516, 2601, 3762
AcyI GRCGYC 1 cut(s) 3865
AdeI CACNNNGTG 1 cut(s) 2592
AfaI GTAC 6 cut(s) 1066, 1560, 1747, 2451, 2839, 4044
AfiI CCNNNNNNNGG 7 cut(s) 168, 1536, 2087, 2753, 3528, 3746, 4147
AflII CTTAAG 1 cut(s) 4304
AflIII ACRYGT 1 cut(s) 3115
AjiI CACGTC 1 cut(s) 3116
AjnI CCWGG 3 cut(s) 2605, 2746, 3160
AjuI GAANNNNNNNTTGG 2 cut(s) 1419, 1451
Alw21I GWGCWC 3 cut(s) 1985, 2688, 3297
Alw26I GTCTC 8 cut(s) 36, 683, 821, 962, 1778, 1916, 3226, 3228
AlwI GGATC 3 cut(s) 3827, 3953, 4240
AlwNI CAGNNNCTG 2 cut(s) 4235, 4262
Ama87I CYCGRG 4 cut(s) 249, 959, 3521, 4092
Aor13HI TCCGGA 1 cut(s) 862
AoxI GGCC 2 cut(s) 2816, 3320
ApeKI GCWGC 9 cut(s) 299, 1865, 2845, 2936, 2939, 3568, 3701, 3856, 4235
ApoI RAATTY 9 cut(s) 93, 178, 1592, 1682, 2255, 2651, 2797, 3155, 4367
AseI ATTAAT 1 cut(s) 810
Asp700I GAANNNNTTC 6 cut(s) 643, 1596, 1673, 2160, 2492, 2529
Asp718I GGTACC 1 cut(s) 1558
AspLEI GCGC 1 cut(s) 3252
AspS9I GGNCC 3 cut(s) 123, 1890, 3330
AsuC2I CCSGG 6 cut(s) 2328, 3522, 3523, 4093, 4094, 4177
AsuII TTCGAA 2 cut(s) 1160, 1277
AvaI CYCGRG 4 cut(s) 249, 959, 3521, 4092
AvaII GGWCC 3 cut(s) 123, 1890, 3330
BaeGI GKGCMC 2 cut(s) 1543, 3189
BanI GGYRCC 2 cut(s) 1540, 1558
BanII GRGCYC 2 cut(s) 2688, 3297
BauI CACGAG 3 cut(s) 2133, 2219, 4295
BbsI GAAGAC 3 cut(s) 630, 666, 2765
Bbv12I GWGCWC 3 cut(s) 1985, 2688, 3297
BbvCI CCTCAGC 1 cut(s) 4263
BbvI GCAGC 9 cut(s) 286, 1852, 2832, 2948, 2951, 3580, 3713, 3843, 4222
BceAI ACGGC 1 cut(s) 1860
BcgI CGANNNNNNTGC 2 cut(s) 3176, 3210
BciT130I CCWGG 3 cut(s) 2607, 2748, 3162
BciVI GTATCC 2 cut(s) 2637, 4219
BclI TGATCA 3 cut(s) 873, 1189, 1306
BcnI CCSGG 6 cut(s) 2328, 3522, 3523, 4093, 4094, 4177
BcoDI GTCTC 8 cut(s) 36, 683, 821, 962, 1778, 1916, 3226, 3228
BfaI CTAG 4 cut(s) 168, 2231, 2522, 2706
BfmI CTRYAG 1 cut(s) 4254
BfrI CTTAAG 1 cut(s) 4304
BfuAI ACCTGC 3 cut(s) 792, 3353, 4261
BfuI GTATCC 2 cut(s) 2637, 4219
BglII AGATCT 1 cut(s) 2527
BisI GCNGC 9 cut(s) 300, 1866, 2846, 2937, 2940, 3569, 3702, 3857, 4236
BlpI GCTNAGC 2 cut(s) 1979, 3129
BlsI GCNGC 9 cut(s) 301, 1867, 2847, 2938, 2941, 3570, 3703, 3858, 4237
BmcAI AGTACT 1 cut(s) 1066
Bme1390I CCNGG 9 cut(s) 2328, 2607, 2748, 3162, 3522, 3523, 4093, 4094, 4177
Bme18I GGWCC 3 cut(s) 123, 1890, 3330
BmeT110I CYCGRG 4 cut(s) 249, 959, 3521, 4092
BmgBI CACGTC 1 cut(s) 3116
BmgT120I GGNCC 3 cut(s) 123, 1890, 3330
BmiI GGNNCC 6 cut(s) 1542, 1560, 2284, 2495, 3499, 4064
BmrFI CCNGG 9 cut(s) 2328, 2607, 2748, 3162, 3522, 3523, 4093, 4094, 4177
BmrI ACTGGG 4 cut(s) 1098, 2763, 2931, 3718
BmsI GCATC 7 cut(s) 2437, 2717, 2923, 2950, 3039, 3910, 4201
BmuI ACTGGG 4 cut(s) 1098, 2763, 2931, 3718
BpiI GAAGAC 3 cut(s) 630, 666, 2765
BplI GAGNNNNNCTC 2 cut(s) 149, 181
Bpu10I CCTNAGC 2 cut(s) 3527, 4263
Bpu1102I GCTNAGC 2 cut(s) 1979, 3129
Bpu14I TTCGAA 2 cut(s) 1160, 1277
BpuEI CTTGAG 2 cut(s) 2933, 3250
BpuMI CCSGG 6 cut(s) 2328, 3522, 3523, 4093, 4094, 4177
BsaAI YACGTR 1 cut(s) 2837
BsaBI GATNNNNATC 1 cut(s) 2625
BsaHI GRCGYC 1 cut(s) 3865
BsaJI CCNNGG 5 cut(s) 1695, 2605, 3349, 3521, 4092
BsaWI WCCGGW 1 cut(s) 862
Bsc4I CCNNNNNNNGG 7 cut(s) 168, 1536, 2087, 2753, 3528, 3746, 4147
Bse1I ACTGG 7 cut(s) 1053, 1093, 2758, 2785, 2926, 3713, 4338
Bse3DI GCAATG 4 cut(s) 2023, 3205, 3377, 3836
Bse8I GATNNNNATC 1 cut(s) 2625
BseAI TCCGGA 1 cut(s) 862
BseBI CCWGG 3 cut(s) 2607, 2748, 3162
BseDI CCNNGG 5 cut(s) 1695, 2605, 3349, 3521, 4092
BseJI GATNNNNATC 1 cut(s) 2625
BseLI CCNNNNNNNGG 7 cut(s) 168, 1536, 2087, 2753, 3528, 3746, 4147
BseMI GCAATG 4 cut(s) 2023, 3205, 3377, 3836
BseNI ACTGG 7 cut(s) 1053, 1093, 2758, 2785, 2926, 3713, 4338
BseSI GKGCMC 2 cut(s) 1543, 3189
BseXI GCAGC 9 cut(s) 286, 1852, 2832, 2948, 2951, 3580, 3713, 3843, 4222
BsgI GTGCAG 2 cut(s) 962, 1073
BshFI GGCC 2 cut(s) 2818, 3322
BshNI GGYRCC 2 cut(s) 1540, 1558
BsiHKAI GWGCWC 3 cut(s) 1985, 2688, 3297
BsiHKCI CYCGRG 4 cut(s) 249, 959, 3521, 4092
BsiSI CCGG 5 cut(s) 863, 2328, 3522, 4093, 4177
BslFI GGGAC 5 cut(s) 1226, 1343, 1520, 2609, 4122
BslI CCNNNNNNNGG 7 cut(s) 168, 1536, 2087, 2753, 3528, 3746, 4147
BsmAI GTCTC 8 cut(s) 36, 683, 821, 962, 1778, 1916, 3226, 3228
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 5 cut(s) 1226, 1343, 1520, 2609, 4122
BsmI GAATGC 4 cut(s) 898, 922, 1847, 3412
BsnI GGCC 2 cut(s) 2818, 3322
BsoBI CYCGRG 4 cut(s) 249, 959, 3521, 4092
Bsp119I TTCGAA 2 cut(s) 1160, 1277
Bsp1286I GDGCHC 5 cut(s) 1543, 1985, 2688, 3189, 3297
Bsp13I TCCGGA 1 cut(s) 862
Bsp1407I TGTACA 1 cut(s) 1745
Bsp1720I GCTNAGC 2 cut(s) 1979, 3129
BspACI CCGC 4 cut(s) 302, 1268, 2263, 4148
BspANI GGCC 2 cut(s) 2818, 3322
BspEI TCCGGA 1 cut(s) 862
BspLI GGNNCC 6 cut(s) 1542, 1560, 2284, 2495, 3499, 4064
BspMAI CTGCAG 1 cut(s) 4258
BspMI ACCTGC 3 cut(s) 792, 3353, 4261
BspPI GGATC 3 cut(s) 3827, 3953, 4240
BspT104I TTCGAA 2 cut(s) 1160, 1277
BspT107I GGYRCC 2 cut(s) 1540, 1558
BspTI CTTAAG 1 cut(s) 4304
BsrDI GCAATG 4 cut(s) 2023, 3205, 3377, 3836
BsrGI TGTACA 1 cut(s) 1745
BsrI ACTGG 7 cut(s) 1053, 1093, 2758, 2785, 2926, 3713, 4338
BssECI CCNNGG 5 cut(s) 1695, 2605, 3349, 3521, 4092
BssNI GRCGYC 1 cut(s) 3865
BssSI CACGAG 3 cut(s) 2133, 2219, 4295
BssT1I CCWWGG 2 cut(s) 1695, 3349
Bst2BI CACGAG 3 cut(s) 2133, 2219, 4295
Bst2UI CCWGG 3 cut(s) 2607, 2748, 3162
Bst6I CTCTTC 9 cut(s) 308, 1074, 1959, 2166, 2483, 2853, 3885, 4155, 4275
BstACI GRCGYC 1 cut(s) 3865
BstAFI CTTAAG 1 cut(s) 4304
BstAUI TGTACA 1 cut(s) 1745
BstBAI YACGTR 1 cut(s) 2837
BstBI TTCGAA 2 cut(s) 1160, 1277
BstC8I GCNNGC 6 cut(s) 135, 1056, 2263, 2816, 3447, 4083
BstHHI GCGC 1 cut(s) 3252
BstMAI GTCTC 8 cut(s) 36, 683, 821, 962, 1778, 1916, 3226, 3228
BstMWI GCNNNNNNNGC 3 cut(s) 917, 3514, 3523
BstNI CCWGG 3 cut(s) 2607, 2748, 3162
BstNSI RCATGY 2 cut(s) 2744, 3488
BstSCI CCNGG 9 cut(s) 2326, 2605, 2746, 3160, 3520, 3521, 4091, 4092, 4175
BstSFI CTRYAG 1 cut(s) 4254
BstSLI GKGCMC 2 cut(s) 1543, 3189
BstV1I GCAGC 9 cut(s) 286, 1852, 2832, 2948, 2951, 3580, 3713, 3843, 4222
BstV2I GAAGAC 3 cut(s) 630, 666, 2765
BstX2I RGATCY 2 cut(s) 2527, 4245
BstYI RGATCY 2 cut(s) 2527, 4245
BsuI GTATCC 2 cut(s) 2637, 4219
BsuRI GGCC 2 cut(s) 2818, 3322
BtrI CACGTC 1 cut(s) 3116
BtsI GCAGTG 1 cut(s) 772
BtsIMutI CAGTG 8 cut(s) 91, 772, 837, 876, 2286, 2586, 2919, 3830
BveI ACCTGC 3 cut(s) 792, 3353, 4261
Cac8I GCNNGC 6 cut(s) 135, 1056, 2263, 2816, 3447, 4083
CaiI CAGNNNCTG 2 cut(s) 4235, 4262
CfoI GCGC 1 cut(s) 3252
Cfr13I GGNCC 3 cut(s) 123, 1890, 3330
Cfr9I CCCGGG 2 cut(s) 3521, 4092
Csp6I GTAC 6 cut(s) 1065, 1559, 1746, 2450, 2838, 4043
CviQI GTAC 6 cut(s) 1065, 1559, 1746, 2450, 2838, 4043
DraIII CACNNNGTG 1 cut(s) 2592
DrdI GACNNNNNNGTC 1 cut(s) 2406
DseDI GACNNNNNNGTC 1 cut(s) 2406
Eam1104I CTCTTC 9 cut(s) 308, 1074, 1959, 2166, 2483, 2853, 3885, 4155, 4275
EarI CTCTTC 9 cut(s) 308, 1074, 1959, 2166, 2483, 2853, 3885, 4155, 4275
Ecl136II GAGCTC 2 cut(s) 2686, 3295
Eco130I CCWWGG 2 cut(s) 1695, 3349
Eco147I AGGCCT 1 cut(s) 3322
Eco24I GRGCYC 2 cut(s) 2688, 3297
Eco47I GGWCC 3 cut(s) 123, 1890, 3330
Eco53kI GAGCTC 2 cut(s) 2686, 3295
Eco57I CTGAAG 8 cut(s) 95, 1098, 1227, 1344, 1521, 2516, 2601, 3762
Eco88I CYCGRG 4 cut(s) 249, 959, 3521, 4092
EcoICRI GAGCTC 2 cut(s) 2686, 3295
EcoRI GAATTC 1 cut(s) 4367
EcoRII CCWGG 3 cut(s) 2605, 2746, 3160
EcoT14I CCWWGG 2 cut(s) 1695, 3349
EcoT22I ATGCAT 1 cut(s) 2732
EcoT38I GRGCYC 2 cut(s) 2688, 3297
ErhI CCWWGG 2 cut(s) 1695, 3349
Esp3I CGTCTC 1 cut(s) 36
FalI AAGNNNNNCTT 6 cut(s) 3078, 3110, 3212, 3244, 3501, 3533
FaqI GGGAC 5 cut(s) 1226, 1343, 1520, 2609, 4122
FauI CCCGC 2 cut(s) 2270, 4141
FauNDI CATATG 2 cut(s) 1702, 1927
FbaI TGATCA 3 cut(s) 873, 1189, 1306
FblI GTMKAC 1 cut(s) 3674
Fnu4HI GCNGC 9 cut(s) 300, 1866, 2846, 2937, 2940, 3569, 3702, 3857, 4236
FriOI GRGCYC 2 cut(s) 2688, 3297
Fsp4HI GCNGC 9 cut(s) 300, 1866, 2846, 2937, 2940, 3569, 3702, 3857, 4236
FspBI CTAG 4 cut(s) 168, 2231, 2522, 2706
GlaI GCGC 1 cut(s) 3251
GluI GCNGC 9 cut(s) 300, 1866, 2846, 2937, 2940, 3569, 3702, 3857, 4236
HaeIII GGCC 2 cut(s) 2818, 3322
HapII CCGG 5 cut(s) 863, 2328, 3522, 4093, 4177
HhaI GCGC 1 cut(s) 3252
Hin1I GRCGYC 1 cut(s) 3865
Hin6I GCGC 1 cut(s) 3250
HinP1I GCGC 1 cut(s) 3250
HindIII AAGCTT 2 cut(s) 854, 3515
HpaII CCGG 5 cut(s) 863, 2328, 3522, 4093, 4177
Hpy166II GTNNAC 5 cut(s) 353, 1893, 2919, 3490, 3675
Hpy8I GTNNAC 5 cut(s) 353, 1893, 2919, 3490, 3675
HpyCH4IV ACGT 5 cut(s) 2836, 3115, 3677, 3865, 4053
HpyF10VI GCNNNNNNNGC 3 cut(s) 917, 3514, 3523
HpySE526I ACGT 5 cut(s) 2836, 3115, 3677, 3865, 4053
Hsp92I GRCGYC 1 cut(s) 3865
HspAI GCGC 1 cut(s) 3250
Kpn2I TCCGGA 1 cut(s) 862
KpnI GGTACC 1 cut(s) 1562
Ksp22I TGATCA 3 cut(s) 873, 1189, 1306
Lsp1109I GCAGC 9 cut(s) 286, 1852, 2832, 2948, 2951, 3580, 3713, 3843, 4222
LweI GCATC 7 cut(s) 2437, 2717, 2923, 2950, 3039, 3910, 4201
MaeI CTAG 4 cut(s) 168, 2231, 2522, 2706
MaeII ACGT 5 cut(s) 2836, 3115, 3677, 3865, 4053
MaeIII GTNAC 6 cut(s) 2076, 2270, 2663, 3209, 3634, 4054
MfeI CAATTG 1 cut(s) 1812
MflI RGATCY 2 cut(s) 2527, 4245
MhlI GDGCHC 5 cut(s) 1543, 1985, 2688, 3189, 3297
MlyI GAGTC 3 cut(s) 167, 2671, 2755
MmeI TCCRAC 3 cut(s) 36, 150, 4006
Mph1103I ATGCAT 1 cut(s) 2732
MroI TCCGGA 1 cut(s) 862
MroXI GAANNNNTTC 6 cut(s) 643, 1596, 1673, 2160, 2492, 2529
MslI CAYNNNNRTG 4 cut(s) 147, 153, 225, 2637
MspA1I CMGCKG 1 cut(s) 3704
MspCI CTTAAG 1 cut(s) 4304
MspI CCGG 5 cut(s) 863, 2328, 3522, 4093, 4177
MspR9I CCNGG 9 cut(s) 2328, 2607, 2748, 3162, 3522, 3523, 4093, 4094, 4177
MunI CAATTG 1 cut(s) 1812
Mva1269I GAATGC 4 cut(s) 898, 922, 1847, 3412
MvaI CCWGG 3 cut(s) 2607, 2748, 3162
MwoI GCNNNNNNNGC 3 cut(s) 917, 3514, 3523
NciI CCSGG 6 cut(s) 2328, 3522, 3523, 4093, 4094, 4177
NdeI CATATG 2 cut(s) 1702, 1927
NlaIV GGNNCC 6 cut(s) 1542, 1560, 2284, 2495, 3499, 4064
NmuCI GTSAC 2 cut(s) 2663, 3634
NsiI ATGCAT 1 cut(s) 2732
NspI RCATGY 2 cut(s) 2744, 3488
NspV TTCGAA 2 cut(s) 1160, 1277
PaeR7I CTCGAG 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 4261
PceI AGGCCT 1 cut(s) 3322
PcsI WCGNNNNNNNCGW 1 cut(s) 2547
PctI GAATGC 4 cut(s) 898, 922, 1847, 3412
PdmI GAANNNNTTC 6 cut(s) 643, 1596, 1673, 2160, 2492, 2529
PkrI GCNGC 9 cut(s) 301, 1867, 2847, 2938, 2941, 3570, 3703, 3858, 4237
PleI GAGTC 3 cut(s) 166, 2670, 2755
PpsI GAGTC 3 cut(s) 166, 2670, 2755
Ppu21I YACGTR 1 cut(s) 2837
PshBI ATTAAT 1 cut(s) 810
PsiI TTATAA 1 cut(s) 263
Psp124BI GAGCTC 2 cut(s) 2688, 3297
Psp6I CCWGG 3 cut(s) 2605, 2746, 3160
PspGI CCWGG 3 cut(s) 2605, 2746, 3160
PspN4I GGNNCC 6 cut(s) 1542, 1560, 2284, 2495, 3499, 4064
PspPI GGNCC 3 cut(s) 123, 1890, 3330
PstI CTGCAG 1 cut(s) 4258
PstNI CAGNNNCTG 2 cut(s) 4235, 4262
PsuI RGATCY 2 cut(s) 2527, 4245
PvuII CAGCTG 1 cut(s) 3704
RsaI GTAC 6 cut(s) 1066, 1560, 1747, 2451, 2839, 4044
RsaNI GTAC 6 cut(s) 1065, 1559, 1746, 2450, 2838, 4043
RseI CAYNNNNRTG 4 cut(s) 147, 153, 225, 2637
SacI GAGCTC 2 cut(s) 2688, 3297
SatI GCNGC 9 cut(s) 300, 1866, 2846, 2937, 2940, 3569, 3702, 3857, 4236
Sau96I GGNCC 3 cut(s) 123, 1890, 3330
ScaI AGTACT 1 cut(s) 1066
SchI GAGTC 3 cut(s) 167, 2671, 2755
ScrFI CCNGG 9 cut(s) 2328, 2607, 2748, 3162, 3522, 3523, 4093, 4094, 4177
SduI GDGCHC 5 cut(s) 1543, 1985, 2688, 3189, 3297
SfaNI GCATC 7 cut(s) 2437, 2717, 2923, 2950, 3039, 3910, 4201
SfcI CTRYAG 1 cut(s) 4254
Sfr274I CTCGAG 1 cut(s) 249
SfuI TTCGAA 2 cut(s) 1160, 1277
SinI GGWCC 3 cut(s) 123, 1890, 3330
SlaI CTCGAG 1 cut(s) 249
SmaI CCCGGG 2 cut(s) 3523, 4094
SmiMI CAYNNNNRTG 4 cut(s) 147, 153, 225, 2637
SmlI CTYRAG 4 cut(s) 249, 2912, 3229, 4304
SmoI CTYRAG 4 cut(s) 249, 2912, 3229, 4304
SseBI AGGCCT 1 cut(s) 3322
SsiI CCGC 4 cut(s) 302, 1268, 2263, 4148
SspMI CTAG 4 cut(s) 168, 2231, 2522, 2706
SstI GAGCTC 2 cut(s) 2688, 3297
StuI AGGCCT 1 cut(s) 3322
StyD4I CCNGG 9 cut(s) 2326, 2605, 2746, 3160, 3520, 3521, 4091, 4092, 4175
StyI CCWWGG 2 cut(s) 1695, 3349
TaiI ACGT 5 cut(s) 2839, 3118, 3680, 3868, 4056
TaqII GACCGA 1 cut(s) 2502
TatI WGTACW 3 cut(s) 1064, 1745, 4042
TscAI CASTG 8 cut(s) 91, 772, 837, 883, 2293, 2593, 2926, 3830
TseFI GTSAC 2 cut(s) 2663, 3634
TseI GCWGC 9 cut(s) 299, 1865, 2845, 2936, 2939, 3568, 3701, 3856, 4235
Tsp45I GTSAC 2 cut(s) 2663, 3634
TspGWI ACGGA 1 cut(s) 2154
TspMI CCCGGG 2 cut(s) 3521, 4092
TspRI CASTG 8 cut(s) 91, 772, 837, 883, 2293, 2593, 2926, 3830
Vha464I CTTAAG 1 cut(s) 4304
VpaK11BI GGWCC 3 cut(s) 123, 1890, 3330
VspI ATTAAT 1 cut(s) 810
XapI RAATTY 9 cut(s) 93, 178, 1592, 1682, 2255, 2651, 2797, 3155, 4367
XceI RCATGY 2 cut(s) 2744, 3488
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 249
XmaI CCCGGG 2 cut(s) 3521, 4092
XmiI GTMKAC 1 cut(s) 3674
XmnI GAANNNNTTC 6 cut(s) 643, 1596, 1673, 2160, 2492, 2529
XspI CTAG 4 cut(s) 168, 2231, 2522, 2706
ZraI GACGTC 1 cut(s) 3866
ZrmI AGTACT 1 cut(s) 1066
Zsp2I ATGCAT 1 cut(s) 2732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.