Rroxscaffold_2G00097510

SNF2 domain-containing protein CLASSY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001072
Physical Location & Seq
Forward (+)
18722118 .. 18727936
5819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097510.1

Sequence Viewer

Length: 3849 bp
ATGGATTATAGGCCTGTATCAAGCAGAACAAGGTCGAAGAGAGACAAAGCTATCATTGAACTTCATTGGGAAATGAAAATTAACAGTGGGGTGAGTCCAGCCTCAAGGGATAAGAAAATGAGGAGGTCCAACCAGGCTTCTGTTTCTCATATGGATGAGTCCAGTTCTAGGAGACAGAGTTTTGAGTGGGTTGCTGATCACGTGGAATCCCTGGAAAACCAACAGAATGATGATGATTCAGAGAGTGTTTTAAAAAGAAGTGGGAAACAGGGAAAGAGGAAAGGAGTTGCGGTTGGTGTGAAGAGAAAGAGGAGTTTAGGATTAGATGTGTCGGCTGATAGCAACAAAGATAGTTTCCATTGGAGAGCTGTTTCGGATAATGGTGTGGAAGTTGTCGATTTGGTGGATAGACCTGATGATGATGAGGTACTAGTTTCTAAAGGTAAAGAGGGAGGAGTAGGAGTTGATGTTGATGTTGATGGTGTGAGTGATGATGAAAGTGATGAGGATGTTGACGATGTTCGTGATAATAAGAGTGATGAGGATGTTTCATCAGCAGAGGAAGATGACAGTAGTGACGAAAGTGTGTTCTCTACAGAGGAGGAACATGAATGTGTTTCCTCATCAGTTGAAGATAGTAGTGAGCAGGAAAATGAAGAGGAGAGTGAGGTGACGGAGAAGAATGAGAAAGCGTGCAAGGGAGAAAGTGTTGAGGTTGATTGGAAGAGAAAGATAAATGCTGATGGCGATGTTCTGCATTATTGTTCTGCTCATGATACTGATACTGATGTCAGAAGTGAGCAAGTGTGGAAGGAAGAAAGCCTTGGGATTCATCCGAAGGGAAAGATTAATGCTGATGGGGATGATCTGCATTCTTCTTCTGATCATGATATCAATGACAGAAGGGCAAAATCGGGGGAGGCAGAGAGTGTGTTGGTTACCTCCCGTACTAAAAAAAAGAGCACGAGGGTACCAAAACAGAAAAGATTACGTACTATTGCATTTGAAGCCCCAAAAGTTGAATGTCTCCCTTTGAGATTCAGTTTTGCTAAGCAGCCAACTATTCCTGAGAAGCCAGATTATCCTGAGAAGCCAGATTATGATAGAGAAGTTGATGCCCTTCTTGCTGAATATAAATTTGCTATAGCATATGAGAATGCTTCTACTGATTCTAAGTTTGAAGAGTTCTACCAAAAGGGTTATTGGTTTTTACTGTTTGACACAAACTTTACTGATACCCAGGCTAAAGACAGCTACTTTACAAGATCAGATAATAACAGAAATGATGTCAATGATGTGACTTATGGTGGTAGTCCAGAAACGGCAATTGACAATGGGAGAACAATTGGTTTGCATGCTTTCAATGAGAATGAAGCTGCCAAAGGAAACAACTTTTCAAGGTCCTGTAATTCACGAAAGGATGACAAAGATGAGACATATGGTGGTAGTCGTCCAGAAACATATAAGAAGAAAGAAAGGGTGGGGAATCTTACCAACAAAAAGGGCATTACAGCACCAAAAGATTGTAATGCCTTTATGATTCTTGTAGATTCGATTTATGACAAGGGAGAAGTGTCAGGGGAACTACCTCTGTTTTGGGATGAATCCCCCCAAGATGAAGCTCCAAGAGATGAGCCTCTTCCATTGTTTTTCACGCTTGGTCATCCTCCAAGTCCTGCACCAGAAAAGTCAGCTTCTGAGATAGAACTAGAAAACCTTTTTGAAGAATATGACTTTGTCCAAATGTCTGAAGAGATTGGTTCCACTGATTCTAAACTGGTTGAAACTAATGACTCAGTCCCTCCCGGTACGGAAGTTACTCAGTATATGCTTTGTAGTCAAGGGAAACACGATCTTATACTTGATGAAGAAATTGGACTCATATGCCAATTCTGCCCCCATGTGCATCGGGAGATCAAGTATATTGTACCAGACTTTGCTCCAAACCCTTATGGAAAAGCTAGGAAGAGATTTTATGAAGAAGACAACCGGTCTATCCTTGATCATCTTCAGTGCAATGACCCTGACAATTGTGGCTTTAGTTCCCATGCTCATACTAAAGGCACGGTGTGGGACTTGATCCCTGGTGTGGAAAGTGATATGTACCCTCATCAGCGTGAAGGTTTTGAATTTATTTGGAGTCACTTAGCTGGTGGAATTCATCTTGGAAAGTTGGCAAAACCTAGTGCTTATGGCGGTGATGGATGCATTATATCGCATGCACCTGGAACTGGGAAGACTCGTTTGACTATTATCTTTATCCAATCGTACATGAATTTTAATCCATCAAGCAGGCCACTCATTGTTGCTCCACGTACCATGCTTCTTACTTGGGAAGAGGAGTTTAAGAAATGGGGTTTTGGCATTCCCTTTCACAATCTTAACAATCCTGACTTCACTGGCAAAGAGGATGAAGCAGCTCGCAATCTTGTGAAGGATGCTAAAAGTTCAGAAATGATAAACTTGACCCGTATCGTGAAGCTAAAGTCCTGGGCAGAGAAAAGAAGCATCTTGGGTATTAGTTATTCACTGTTTGAGCAGATTTGTGGAGCTCCCATCAAGAAAAGAAAACATCCACTTTCTCCAAGACGAAAGCTCGAAGCTGAGCAGATGTGGAAAGTCCTTGTTGAATTTCCTGGTCTTGTAGTATTTGACGAAGGGCACAATCCTCGCAACGACCAGAGTTACTCATGGAATGCTCTTTTAAAACTGAAAACAAGAAGGCGCATTCTCCTCTCTGGTACTCCTTTTCAGAATAATTTTGAGGAGCTCTACAATACAGTCCGCTTGGTGAGGCCAGAATGGACCACCAATCCTACCTGCCTTGGAGCTTTTGACAAAAAGCAACGGACTTGGGGCCAAATGCGAGGCAGAAAAGGCAATGCTGGGAAAGGCCAATTTGCTGATGATAATACAAAACGAGCTAAAGAGGTCAGAGCTATGATTTCTCCATTTGTGCATGTTTACAAAGGCAACATACTGCAAGATAAGCTTCCCGGGCTAAGGAAATCAGTTGTTGTTTTACAGCCAACAGCTTTACAGCAGAGATGTTCTGACAGAATTCAGCTGATAAAGAATCCATTTGAATATGAACGAATAGAGGCTAAAGTAACTATCCACCCTTCTCTCTTGCTTAATGAAAAGCGCTCTATGTTTCTTGAGGAAGACAAGTATTCAGGTGACTGGGAAAGGCTAACAGAAATCAAGTTAAATCCTGAAGGTGGAGTGAAGGCAAGATTTGCAGTTGATCTCATTCGGCTTTGTCAAGCTATGAATGAAAGAGTTTTGGTATTCAGTCAGTGGGTTGATCCATTGCTCTTAATAAGGGACCTGCTCAAATCTCAGTTTCAGTGGGTGGAAGGAGAGGAGGTTATGTATATGGATGGAAAATGTGATGCAAAGCAGCGCCAATCCTCAATGAGTTATTTCAATGATCCATCCACCAAAGCAAAGGTTATGTTAGCTACAACAAGAGGTTGCAACGAAGGAATAAGTTTAGTTGGAGCTTCAAGGGTTGTACTGCTTGATGTTGCATGGAACCCTTCTGTTGAGAGGCAAGCTATATCCCGAGCATACCGATTGGGACAGAAAAAAATTGTGTTCGTCTACCATCTTCTCATGGCTGGGGCAAGAGAAGAGGACAAATACAACCGACAAGTTGAGAAGTATCGGTTGTCTGAATTGGTATTTTCTTGTTCGGATACAGAAGCTGATGCCGAGAAAATCCCAGCTTCAGTCGCGGAGGACAAGATTTTGGAAGAGATGGTTCAGCACCAGAAACTGAAGCATATATTTCAAAAGATAACATTACTGAATGAGGCTTCATATTTGGACGAAGAATTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1282

Amino Acids

145.72

Weight (kDa)

5.38

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 701 - 917 5.9e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 704 - 1025 5e-24 SNF2-related domain
Helicase_C PF00271 1083 - 1195 5.1e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2828, 3339
Acc65I GGTACC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 970
AccI GTMKAC 1 cut(s) 3606
AccII CGCG 1 cut(s) 3740
AciI CCGC 4 cut(s) 290, 2196, 2785, 3740
AclWI GGATC 3 cut(s) 2074, 3302, 3428
AcsI RAATTY 7 cut(s) 1136, 2129, 2157, 2275, 2630, 3060, 3839
AcuI CTGAAG 5 cut(s) 1770, 1994, 3237, 3717, 3803
AcvI CACGTG 1 cut(s) 202
AdeI CACNNNGTG 1 cut(s) 2070
AfeI AGCGCT 1 cut(s) 3146
AfiI CCNNNNNNNGG 5 cut(s) 168, 2089, 2231, 3003, 3221
AgeI ACCGGT 1 cut(s) 1989
AhdI GACNNNNNGTC 1 cut(s) 1990
AhlI ACTAGT 1 cut(s) 430
AjnI CCWGG 7 cut(s) 132, 210, 1239, 2083, 2224, 2489, 2635
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
Alw21I GWGCWC 3 cut(s) 965, 2554, 2772
Alw26I GTCTC 4 cut(s) 36, 166, 1031, 1427
AlwI GGATC 3 cut(s) 2074, 3302, 3428
AlwNI CAGNNNCTG 3 cut(s) 1697, 3710, 3781
Ama87I CYCGRG 2 cut(s) 2996, 3567
Aor51HI AGCGCT 1 cut(s) 3146
AoxI GGCC 5 cut(s) 11, 2294, 2795, 2857, 2893
ApeKI GCWGC 4 cut(s) 1054, 1376, 2417, 3403
ApoI RAATTY 7 cut(s) 1136, 2129, 2157, 2275, 2630, 3060, 3839
ArsI GACNNNNNNTTYG 1 cut(s) 3824
AseI ATTAAT 1 cut(s) 849
AsiGI ACCGGT 1 cut(s) 1989
Asp718I GGTACC 1 cut(s) 970
AspLEI GCGC 3 cut(s) 2727, 3147, 3408
AspS9I GGNCC 5 cut(s) 126, 1401, 2805, 2857, 3328
AsuC2I CCSGG 3 cut(s) 1806, 2997, 2998
AsuHPI GGTGA 5 cut(s) 103, 682, 2210, 2803, 3191
AvaI CYCGRG 2 cut(s) 2996, 3567
AvaII GGWCC 4 cut(s) 126, 1401, 2805, 3328
BaeGI GKGCMC 1 cut(s) 2664
BaeI ACNNNNGTAYC 6 cut(s) 774, 807, 954, 987, 1800, 1833
BanI GGYRCC 1 cut(s) 970
BanII GRGCYC 2 cut(s) 2554, 2772
BauI CACGAG 1 cut(s) 964
BbrPI CACGTG 1 cut(s) 202
BbsI GAAGAC 3 cut(s) 1989, 2243, 3171
Bbv12I GWGCWC 3 cut(s) 965, 2554, 2772
BbvI GCAGC 4 cut(s) 1066, 1363, 2429, 3415
BceAI ACGGC 1 cut(s) 1338
BcgI CGANNNNNNTGC 2 cut(s) 2651, 2685
BciT130I CCWGG 7 cut(s) 134, 212, 1241, 2085, 2226, 2491, 2637
BciVI GTATCC 1 cut(s) 3694
BclI TGATCA 3 cut(s) 196, 883, 2002
BcnI CCSGG 3 cut(s) 1806, 2997, 2998
BcoDI GTCTC 4 cut(s) 36, 166, 1031, 1427
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 5 cut(s) 168, 431, 1709, 1962, 2184
BfmI CTRYAG 2 cut(s) 594, 1143
BfoI RGCGCY 2 cut(s) 3148, 3409
BfuAI ACCTGC 2 cut(s) 2828, 3339
BfuI GTATCC 1 cut(s) 3694
BisI GCNGC 4 cut(s) 1055, 1377, 2418, 3404
BlpI GCTNAGC 2 cut(s) 1050, 2604
BlsI GCNGC 4 cut(s) 1056, 1378, 2419, 3405
Bme18I GGWCC 4 cut(s) 126, 1401, 2805, 3328
BmeRI GACNNNNNGTC 1 cut(s) 1990
BmeT110I CYCGRG 2 cut(s) 2996, 3567
BmgT120I GGNCC 5 cut(s) 126, 1401, 2805, 2857, 3328
BmiI GGNNCC 5 cut(s) 972, 1762, 2858, 3329, 3539
BmrI ACTGGG 2 cut(s) 2241, 3193
BmsI GCATC 7 cut(s) 1105, 1915, 2195, 2428, 2517, 3385, 3703
BmuI ACTGGG 2 cut(s) 2241, 3193
BpiI GAAGAC 3 cut(s) 1989, 2243, 3171
Bpu10I CCTNAGC 1 cut(s) 3002
Bpu1102I GCTNAGC 2 cut(s) 1050, 2604
BpuEI CTTGAG 2 cut(s) 88, 3179
BpuMI CCSGG 3 cut(s) 1806, 2997, 2998
BsaAI YACGTR 3 cut(s) 202, 992, 2315
BsaJI CCNNGG 7 cut(s) 210, 823, 1239, 2083, 2490, 2824, 2996
BsaWI WCCGGW 1 cut(s) 1989
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 2089, 2231, 3003, 3221
Bse118I RCCGGY 1 cut(s) 1989
Bse1I ACTGG 5 cut(s) 162, 1782, 2236, 2404, 3188
Bse3DI GCAATG 3 cut(s) 2023, 2887, 3311
BseBI CCWGG 7 cut(s) 134, 212, 1241, 2085, 2226, 2491, 2637
BseDI CCNNGG 7 cut(s) 210, 823, 1239, 2083, 2490, 2824, 2996
BseLI CCNNNNNNNGG 5 cut(s) 168, 2089, 2231, 3003, 3221
BseMI GCAATG 3 cut(s) 2023, 2887, 3311
BseMII CTCAG 7 cut(s) 1059, 1077, 1689, 1809, 1835, 2595, 3356
BseNI ACTGG 5 cut(s) 162, 1782, 2236, 2404, 3188
BseRI GAGGAG 9 cut(s) 136, 325, 468, 614, 674, 2354, 2723, 2780, 3380
BseSI GKGCMC 1 cut(s) 2664
BseXI GCAGC 4 cut(s) 1066, 1363, 2429, 3415
BseYI CCCAGC 3 cut(s) 2885, 3623, 3727
BsgI GTGCAG 1 cut(s) 1662
Bsh1236I CGCG 1 cut(s) 3740
BshFI GGCC 5 cut(s) 13, 2296, 2797, 2859, 2895
BshNI GGYRCC 1 cut(s) 970
BshTI ACCGGT 1 cut(s) 1989
BsiHKAI GWGCWC 3 cut(s) 965, 2554, 2772
BsiHKCI CYCGRG 2 cut(s) 2996, 3567
BsiSI CCGG 3 cut(s) 1806, 1990, 2997
BslFI GGGAC 4 cut(s) 1784, 2087, 3341, 3597
BslI CCNNNNNNNGG 5 cut(s) 168, 2089, 2231, 3003, 3221
BsmAI GTCTC 4 cut(s) 36, 166, 1031, 1427
BsmFI GGGAC 4 cut(s) 1784, 2087, 3341, 3597
BsmI GAATGC 5 cut(s) 871, 1162, 2364, 2701, 2727
BsnI GGCC 5 cut(s) 13, 2296, 2797, 2859, 2895
BsoBI CYCGRG 2 cut(s) 2996, 3567
Bsp1286I GDGCHC 4 cut(s) 965, 2554, 2664, 2772
Bsp1720I GCTNAGC 2 cut(s) 1050, 2604
BspACI CCGC 4 cut(s) 290, 2196, 2785, 3740
BspANI GGCC 5 cut(s) 13, 2296, 2797, 2859, 2895
BspCNI CTCAG 7 cut(s) 1060, 1078, 1690, 1808, 1834, 2596, 3355
BspFNI CGCG 1 cut(s) 3740
BspHI TCATGA 2 cut(s) 772, 886
BspLI GGNNCC 5 cut(s) 972, 1762, 2858, 3329, 3539
BspMI ACCTGC 2 cut(s) 2828, 3339
BspPI GGATC 3 cut(s) 2074, 3302, 3428
BspT107I GGYRCC 1 cut(s) 970
BsrDI GCAATG 3 cut(s) 2023, 2887, 3311
BsrFI RCCGGY 1 cut(s) 1989
BsrI ACTGG 5 cut(s) 162, 1782, 2236, 2404, 3188
BssAI RCCGGY 1 cut(s) 1989
BssECI CCNNGG 7 cut(s) 210, 823, 1239, 2083, 2490, 2824, 2996
BssSI CACGAG 1 cut(s) 964
BssT1I CCWWGG 2 cut(s) 823, 2824
Bst2BI CACGAG 1 cut(s) 964
Bst2UI CCWGG 7 cut(s) 134, 212, 1241, 2085, 2226, 2491, 2637
Bst4CI ACNGT 6 cut(s) 86, 572, 1215, 2068, 2532, 2782
BstAPI GCANNNNNTGC 1 cut(s) 3239
BstBAI YACGTR 3 cut(s) 202, 992, 2315
BstC8I GCNNGC 6 cut(s) 694, 1356, 2220, 2294, 2422, 3558
BstEII GGTNACC 1 cut(s) 937
BstFNI CGCG 1 cut(s) 3740
BstH2I RGCGCY 2 cut(s) 3148, 3409
BstHHI GCGC 3 cut(s) 2727, 3147, 3408
BstMAI GTCTC 4 cut(s) 36, 166, 1031, 1427
BstMWI GCNNNNNNNGC 8 cut(s) 1007, 1124, 1893, 2877, 2989, 2998, 3239, 3737
BstNI CCWGG 7 cut(s) 134, 212, 1241, 2085, 2226, 2491, 2637
BstNSI RCATGY 3 cut(s) 1358, 2222, 2963
BstPI GGTNACC 1 cut(s) 937
BstSFI CTRYAG 2 cut(s) 594, 1143
BstSLI GKGCMC 1 cut(s) 2664
BstSNI TACGTA 1 cut(s) 992
BstUI CGCG 1 cut(s) 3740
BstV1I GCAGC 4 cut(s) 1066, 1363, 2429, 3415
BstV2I GAAGAC 3 cut(s) 1989, 2243, 3171
BsuI GTATCC 1 cut(s) 3694
BsuRI GGCC 5 cut(s) 13, 2296, 2797, 2859, 2895
BtgZI GCGATG 1 cut(s) 762
BtsIMutI CAGTG 7 cut(s) 91, 1764, 2018, 2397, 2528, 3305, 3356
BveI ACCTGC 2 cut(s) 2828, 3339
Cac8I GCNNGC 6 cut(s) 694, 1356, 2220, 2294, 2422, 3558
CaiI CAGNNNCTG 3 cut(s) 1697, 3710, 3781
CciI TCATGA 2 cut(s) 772, 886
CfoI GCGC 3 cut(s) 2727, 3147, 3408
Cfr10I RCCGGY 1 cut(s) 1989
Cfr13I GGNCC 5 cut(s) 126, 1401, 2805, 2857, 3328
Cfr9I CCCGGG 1 cut(s) 2996
CspAI ACCGGT 1 cut(s) 1989
DraI TTTAAA 2 cut(s) 252, 2706
DraIII CACNNNGTG 1 cut(s) 2070
DriI GACNNNNNGTC 1 cut(s) 1990
Eam1105I GACNNNNNGTC 1 cut(s) 1990
Ecl136II GAGCTC 2 cut(s) 2552, 2770
Eco105I TACGTA 1 cut(s) 992
Eco130I CCWWGG 2 cut(s) 823, 2824
Eco147I AGGCCT 1 cut(s) 13
Eco24I GRGCYC 2 cut(s) 2554, 2772
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 4 cut(s) 126, 1401, 2805, 3328
Eco47III AGCGCT 1 cut(s) 3146
Eco53kI GAGCTC 2 cut(s) 2552, 2770
Eco57I CTGAAG 5 cut(s) 1770, 1994, 3237, 3717, 3803
Eco72I CACGTG 1 cut(s) 202
Eco88I CYCGRG 2 cut(s) 2996, 3567
Eco91I GGTNACC 1 cut(s) 937
EcoICRI GAGCTC 2 cut(s) 2552, 2770
EcoO109I RGGNCCY 2 cut(s) 1401, 3328
EcoO65I GGTNACC 1 cut(s) 937
EcoRI GAATTC 2 cut(s) 2157, 3060
EcoRII CCWGG 7 cut(s) 132, 210, 1239, 2083, 2224, 2489, 2635
EcoRV GATATC 1 cut(s) 892
EcoT14I CCWWGG 2 cut(s) 823, 2824
EcoT22I ATGCAT 1 cut(s) 2210
EcoT38I GRGCYC 2 cut(s) 2554, 2772
ErhI CCWWGG 2 cut(s) 823, 2824
FalI AAGNNNNNCTT 4 cut(s) 807, 839, 2976, 3008
FaqI GGGAC 4 cut(s) 1784, 2087, 3341, 3597
FauNDI CATATG 4 cut(s) 150, 1150, 1438, 1883
FbaI TGATCA 3 cut(s) 196, 883, 2002
FblI GTMKAC 1 cut(s) 3606
Fnu4HI GCNGC 4 cut(s) 1055, 1377, 2418, 3404
FriOI GRGCYC 2 cut(s) 2554, 2772
Fsp4HI GCNGC 4 cut(s) 1055, 1377, 2418, 3404
FspBI CTAG 5 cut(s) 168, 431, 1709, 1962, 2184
GlaI GCGC 3 cut(s) 2726, 3146, 3407
GluI GCNGC 4 cut(s) 1055, 1377, 2418, 3404
GsaI CCCAGC 3 cut(s) 2889, 3627, 3731
HaeII RGCGCY 2 cut(s) 3148, 3409
HaeIII GGCC 5 cut(s) 13, 2296, 2797, 2859, 2895
HapII CCGG 3 cut(s) 1806, 1990, 2997
HhaI GCGC 3 cut(s) 2727, 3147, 3408
Hin6I GCGC 3 cut(s) 2725, 3145, 3406
HinP1I GCGC 3 cut(s) 2725, 3145, 3406
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 2990
HpaII CCGG 3 cut(s) 1806, 1990, 2997
HphI GGTGA 5 cut(s) 103, 682, 2210, 2803, 3191
Hpy166II GTNNAC 3 cut(s) 514, 2965, 3607
Hpy8I GTNNAC 3 cut(s) 514, 2965, 3607
HpyCH4III ACNGT 6 cut(s) 86, 572, 1215, 2068, 2532, 2782
HpyCH4IV ACGT 3 cut(s) 201, 991, 2314
HpyF10VI GCNNNNNNNGC 8 cut(s) 1007, 1124, 1893, 2877, 2989, 2998, 3239, 3737
HpySE526I ACGT 3 cut(s) 201, 991, 2314
HspAI GCGC 3 cut(s) 2725, 3145, 3406
KpnI GGTACC 1 cut(s) 974
Ksp22I TGATCA 3 cut(s) 196, 883, 2002
LmnI GCTCC 8 cut(s) 1627, 1945, 2314, 2549, 2557, 2767, 2828, 3503
Lsp1109I GCAGC 4 cut(s) 1066, 1363, 2429, 3415
LweI GCATC 7 cut(s) 1105, 1915, 2195, 2428, 2517, 3385, 3703
MaeI CTAG 5 cut(s) 168, 431, 1709, 1962, 2184
MaeII ACGT 3 cut(s) 201, 991, 2314
MaeIII GTNAC 9 cut(s) 575, 670, 937, 1297, 1816, 2141, 2684, 3109, 3179
MfeI CAATTG 3 cut(s) 1326, 1344, 2029
MhlI GDGCHC 4 cut(s) 965, 2554, 2664, 2772
MlyI GAGTC 6 cut(s) 103, 167, 1787, 1872, 2149, 2233
MmeI TCCRAC 2 cut(s) 153, 3481
Mph1103I ATGCAT 1 cut(s) 2210
MslI CAYNNNNRTG 3 cut(s) 153, 612, 2115
MspA1I CMGCKG 1 cut(s) 3067
MspI CCGG 3 cut(s) 1806, 1990, 2997
MunI CAATTG 3 cut(s) 1326, 1344, 2029
Mva1269I GAATGC 5 cut(s) 871, 1162, 2364, 2701, 2727
MvaI CCWGG 7 cut(s) 134, 212, 1241, 2085, 2226, 2491, 2637
MvnI CGCG 1 cut(s) 3740
MwoI GCNNNNNNNGC 8 cut(s) 1007, 1124, 1893, 2877, 2989, 2998, 3239, 3737
NciI CCSGG 3 cut(s) 1806, 2997, 2998
NdeI CATATG 4 cut(s) 150, 1150, 1438, 1883
NlaIV GGNNCC 5 cut(s) 972, 1762, 2858, 3329, 3539
NmeAIII GCCGAG 1 cut(s) 3742
NmuCI GTSAC 5 cut(s) 575, 670, 1297, 2141, 3179
NsiI ATGCAT 1 cut(s) 2210
NspI RCATGY 3 cut(s) 1358, 2222, 2963
PaeI GCATGC 2 cut(s) 1358, 2222
PagI TCATGA 2 cut(s) 772, 886
PceI AGGCCT 1 cut(s) 13
PctI GAATGC 5 cut(s) 871, 1162, 2364, 2701, 2727
PflFI GACNNNGTC 2 cut(s) 1736, 1796
PinAI ACCGGT 1 cut(s) 1989
PkrI GCNGC 4 cut(s) 1056, 1378, 2419, 3405
PleI GAGTC 6 cut(s) 102, 166, 1787, 1872, 2148, 2233
PmaCI CACGTG 1 cut(s) 202
PmlI CACGTG 1 cut(s) 202
PpsI GAGTC 6 cut(s) 102, 166, 1787, 1872, 2148, 2233
Ppu21I YACGTR 3 cut(s) 202, 992, 2315
PpuMI RGGWCCY 2 cut(s) 1401, 3328
PshBI ATTAAT 1 cut(s) 849
Psp124BI GAGCTC 2 cut(s) 2554, 2772
Psp5II RGGWCCY 2 cut(s) 1401, 3328
Psp6I CCWGG 7 cut(s) 132, 210, 1239, 2083, 2224, 2489, 2635
PspCI CACGTG 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 937
PspFI CCCAGC 3 cut(s) 2885, 3623, 3727
PspGI CCWGG 7 cut(s) 132, 210, 1239, 2083, 2224, 2489, 2635
PspN4I GGNNCC 5 cut(s) 972, 1762, 2858, 3329, 3539
PspPI GGNCC 5 cut(s) 126, 1401, 2805, 2857, 3328
PspPPI RGGWCCY 2 cut(s) 1401, 3328
PstNI CAGNNNCTG 3 cut(s) 1697, 3710, 3781
PsyI GACNNNGTC 2 cut(s) 1736, 1796
PvuII CAGCTG 1 cut(s) 3067
RseI CAYNNNNRTG 3 cut(s) 153, 612, 2115
SacI GAGCTC 2 cut(s) 2554, 2772
SatI GCNGC 4 cut(s) 1055, 1377, 2418, 3404
Sau96I GGNCC 5 cut(s) 126, 1401, 2805, 2857, 3328
SchI GAGTC 6 cut(s) 103, 167, 1787, 1872, 2149, 2233
SduI GDGCHC 4 cut(s) 965, 2554, 2664, 2772
SfaNI GCATC 7 cut(s) 1105, 1915, 2195, 2428, 2517, 3385, 3703
SfcI CTRYAG 2 cut(s) 594, 1143
SinI GGWCC 4 cut(s) 126, 1401, 2805, 3328
SmaI CCCGGG 1 cut(s) 2998
SmiMI CAYNNNNRTG 3 cut(s) 153, 612, 2115
SmlI CTYRAG 2 cut(s) 103, 3158
SmoI CTYRAG 2 cut(s) 103, 3158
SnaBI TACGTA 1 cut(s) 992
SpeI ACTAGT 1 cut(s) 430
SphI GCATGC 2 cut(s) 1358, 2222
SseBI AGGCCT 1 cut(s) 13
SsiI CCGC 4 cut(s) 290, 2196, 2785, 3740
SspMI CTAG 5 cut(s) 168, 431, 1709, 1962, 2184
SstI GAGCTC 2 cut(s) 2554, 2772
StuI AGGCCT 1 cut(s) 13
StyI CCWWGG 2 cut(s) 823, 2824
TaaI ACNGT 6 cut(s) 86, 572, 1215, 2068, 2532, 2782
TaiI ACGT 3 cut(s) 204, 994, 2317
TaqI TCGA 4 cut(s) 35, 396, 1553, 2598
TatI WGTACW 1 cut(s) 3517
TscAI CASTG 7 cut(s) 91, 1771, 2018, 2404, 2535, 3305, 3356
TseFI GTSAC 5 cut(s) 575, 670, 1297, 2141, 3179
TseI GCWGC 4 cut(s) 1054, 1376, 2417, 3403
Tsp45I GTSAC 5 cut(s) 575, 670, 1297, 2141, 3179
TspGWI ACGGA 3 cut(s) 689, 1826, 2863
TspMI CCCGGG 1 cut(s) 2996
TspRI CASTG 7 cut(s) 91, 1771, 2018, 2404, 2535, 3305, 3356
Tth111I GACNNNGTC 2 cut(s) 1736, 1796
VpaK11BI GGWCC 4 cut(s) 126, 1401, 2805, 3328
VspI ATTAAT 1 cut(s) 849
XapI RAATTY 7 cut(s) 1136, 2129, 2157, 2275, 2630, 3060, 3839
XceI RCATGY 3 cut(s) 1358, 2222, 2963
XmaI CCCGGG 1 cut(s) 2996
XmiI GTMKAC 1 cut(s) 3606
XspI CTAG 5 cut(s) 168, 431, 1709, 1962, 2184
Zsp2I ATGCAT 1 cut(s) 2210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.