RLG00000020460

SNF2 domain-containing protein CLASSY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001072
Physical Location & Seq
Reverse (-)
65282831 .. 65288633
5803 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020460

Sequence Viewer

Length: 3900 bp
ATGGATTATAGGCCTGTATCTAGCAGAACAAGGTCGAAGAGAGACAAAGCTATCATTGAACATCATTGGGAAATGAAAATTAACAGTGGGGTGAGTCCAGCCTCAAGGGATAAGAAACTGAGGAGGTCCAACCAGGCTTCTGTTTCTCATATGGGTGAGTCCAGTTCTAGGAGACACAGTTTTGAGTGGGTTGCTGATCATGTGGAATCCCTGGAAAATCAACAGAATGATGATGATTCAGAGAGTGTTATAAAAAGATGTGGGAAACAGGGAAAGGGGAAAGGAGTTGCGGTTGGTGTGAAGAGAAAGAGGAGTTTAGGATTAGATGTGTCGGCTGATAGCAACAAAGATGGTTGCCATTGGAGAGCTGTTTCGGATAATGGTGTGGAAGTTGTCGATTTGGTGGATAGTCCTGATGATGAAGGGGTACTAGTTTCTAAAGGTAAAGAGGGAGGAGTAGGAGTTGATGTTGATGGTGTGAGTGATGATGAAAGTGATGAGGATGTTGACGATGTTCGTGATAATAAGAGTGAAGAGGATGTTTCATCAGCAGAGGAAGATGACAGTAGTGACGAAAGTGTGTTCCCTATAGAGGAGGAAAATGAATGTGTTTCCTCATCAGTTGAAGATAGTAGTGAGCAGGAAAATGAAGAGGAGAGTGAGGTGACGGAGAAGAATGAGAAAGCGTGCAAGGGAGAAAGTGTTGAGGTTGATTGGAAGAGAAAGATAAATGCTGATGGTGATGTTCTGCATTATTGTTCTGCTCATGATACTGATACTGATGTCAGAAGTGAGCAAGTGTGGAAGGAAGAAAGTCTTAGGATTCATCCAAAGGGAAAGATTAATGCTGATGGGGATGATCTACATTCTTCTTCTGATCATGATATCAATGACAGAAGGGCAAAATTGGGGGAGGCAGAGAGTGTGTTGGTTACCTCCCGTACTAAAGAAAAGAGCACAAGGGTAACAAAACAGAAAAGATTACCTACTATTGCATTTGAAGCCCCAAAAGTTGAATGTCTCCCTTTGAGATTCAGTTTTGCTAAGCAGCCAACTATTCCTGAGAAGCCAGATTATGAGAGAGAAGTAGATGCCCTTCTTGCTGAATGTAAATTTTCTATAGCATATGAGAATGCTTCTACTGATTCTAAGGCTAAGGACAGCTACTTTACAAGATCAGATAATAACAGAAAGGATGTCAATGATGTGACTTATGGTGGTAGTCCAGAAACGGCAATTGATAATGGGAGAACAATTGGTTTGCAGATGGCTTTCAATGAGAGTGAAGCTGCCAAAGGAGATGATGATGCAAGGTCAAAGAATAGGATACATGATTTCGATGATGAGACGCATGGCGGTGCTTCAGAATCTGGTTCTAATAGAGGCAGTAAATTTGATGTGCACAGGGCTTGCAAGAAGGGTGTAGAGGCCAAAGGAAACAACTTTTCAAGGTCCTTTAACTCACAAAAGGATGACAAAGATGAGACATATGGTGGTAGTCGTCCAGAAACATATAAGAAGAAAGAAAGGGTGGGGAATCTTACCAACAAAAAGGGCATTACAGCACCAAAAGATTGCAATGCCTTTATGATCCTTGTAGATTCGATTTATGACAAGGGAGAAGTGTCAGGGGAACTACCTCCATTTGGGGATGAATCCCCCCAAGATGAAGCTCCAAGAGATGAGCCTCTTCCATTGTTTTTCACGCTTGGTCATCCTCCAAGTCCTGCACCAGAGAAGTCAGCTTCTGAGATAGAACTAGAAAACCTTTTTGAAGAATATGACTTTGTCCAAATGTCTGAAGAGATTGGTTCCACTGATTCTAAACTGGTTGAAACTAATGACTCAGTCCCTCCCGGTACAGAAGTTACTCAGTATATGCTTTGTAGTCAAGGGAAACACGATCTTATACTTGATGAAGAAATTGGACTCATATGCCAATTCTGCCCTCATGTGCATCGGGAGATCAAGTATATTGTACCAGACTTTGCTCGAAACCCTTATGGAAAAGCTGGGAAGAGATTTTATGAAGAAGACAACCCGTCTATCCTTGATGATCTTCAGTGTAATGACCCTGACAATTGTGGCTTTAGTTCGGATGCTCATACTGAAGGCACGGTGTGGGACTTGATCCCTGGTGTGAAAAGTGATATGTACCCTCATCAGCGTGAAGGTTTCGAATTTATTTGGAGTCACTTAGCTGGTGGAATTCAGCTTGGAAAGTTGGCAAAACCTAGTGCTTGTGGCGGTGATGGATGCATTATATCACATGCTCCTGGGACTGGGAAGACTCGTTTGACTATTATCTTTATCCAGTCGTACATGAATTTTAACCCATCAAGCAGGCCACTCATTGTTGCTCCACGTACCATGCTTCTTACTTGGGAAGAGGAGTTTAAGAAATGGGGTTTTGACATTCCCTTTCACAATCTTAACAATCCTGACTTCACTGGCAAAGAGGATGAAGCAGCTCGCAATCTTGTGAAGGATAGTAAAAGTTCAGAAATGATAAACTTGACCCGTATCGTGAAGCTAAAGTCCTGGGCAGAGAAAAGAAGCATCTTGGGTATTAGTTATTCACTGTTTGAGCAGATTTGTGGAGCTTCTACCAAGAAAGGAAAACATCCACTTTCTCCAAGACGAAAGCTTGAAGCTGAGCAGATGTGGAAAGTCCTTGTTGAATTTCCTGGTCTTGTAGTATTAGACGAAGGGCACAATCCTCGCAATGACCAGAGTTACTCATGGAATGCTCTTTTAAAACTGAAAACAAGAAGGCGCATTCTCCTCTCTGGTACTCCTTTTCAGAATAATTTTGAGGAGCTCTACAATACAGTCCGCTTGGTGAGGCCAGAATGGACCACCAATCCTACCTGCCTTGGAGCTTTTGACAAAAAGCAACGGACTTGGGGCCAAATGCAAGGCAGAAAAGGCAATGCTGGGAAAGGCCAATTTGCTGATGATAATACAAAGCGAGCTAAAGAGGTCAAAGCTATGATTTCCCCATTTGTGCATGTTTACAAAGGCACCATACTGCAAGATAAGCTTCCCGGGTTAAGGAACTCAGTTGTTGTTTTACAGCCAACAGCTTTGCAGCAGAAATGTTCTGACAGAATTCAGCCGATAAAGAATCCATTTCAATATGAACGAATAGAGGCTAAAGTAACTATCCACCCTTCTCTCTTGCTTAATGAAAAGCGCTCTATGTTTCTTGAGGAAGACAAGTATTCAGGTGACTGGGAAAGGCTAACAGAAATCAAGTTAAATCCTGAAGGTGGAGTGAAGGCAAGATTTGCAGTTGATCTCATTCGGCTTTGTCACGCTACGAATGAAAGAGTTTTGGTATTCAGTCAGTGGGTTGATCCATTGCTCTTCATAAGGGACCTGCTCAAATCTCAGTTTCAGTGGGTGGAAGGAGAGGAGGTTATGTATATGGATGGAAAATGTGATGCAAAGCAGCGCCAATCCTCAATGAGTTATTTCAATGATCCATCCACCAAAGCAAAGGTTATGTTAGCTACAACAAAAGGTTGCAACGAAGGAATAAGTTTAGTTGGAGCTTCAAGGGTTGTGCTGCTTGATGTTGCATGGAACCCATCTGTTGAGCGGCAAGCTATATCCCGAGCATACCGATTGGGACAGAAAAAAATTGTGTTCATCTACCATCTCCTCATGTCTGGGGCAAGAGAAGAGTACAAATACAGCCGACAAGTTGAGAAGTATCGGTTGTCTGAATTGGTATTTTCTTGTTTGGATACAGAAGCTGATGCCAAGAAAATCCCAGCTGCGGTCGCGGAGGACAAGATTTTGGAAGAGATGGTTCAGCACCAGAAACTGAAGCATATATTTCAAAAGATAACATTACTGAATGAAGCTTCATATTTGGACGAAGAATTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1300

Amino Acids

146.25

Weight (kDa)

5.38

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 718 - 934 3.2e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 721 - 1037 2e-24 SNF2-related domain
Helicase_C PF00271 1099 - 1212 3.1e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 2879, 3390
AccB1I GGYRCC 1 cut(s) 3023
AccBSI CCGCTC 1 cut(s) 3604
AccII CGCG 1 cut(s) 3791
AciI CCGC 7 cut(s) 290, 1356, 2247, 2836, 3604, 3785, 3791
AclWI GGATC 4 cut(s) 1585, 2125, 3353, 3479
AcsI RAATTY 8 cut(s) 1112, 1391, 2180, 2208, 2326, 2681, 3111, 3890
AcuI CTGAAG 6 cut(s) 1347, 1821, 2045, 2130, 3288, 3854
AdeI CACNNNGTG 1 cut(s) 2121
AfaI GTAC 9 cut(s) 429, 943, 1861, 1980, 2156, 2321, 2368, 2794, 3692
AfeI AGCGCT 1 cut(s) 3197
AfiI CCNNNNNNNGG 7 cut(s) 168, 592, 1646, 2282, 3054, 3272, 3784
AhdI GACNNNNNGTC 1 cut(s) 2041
AhlI ACTAGT 1 cut(s) 430
AjnI CCWGG 6 cut(s) 132, 210, 2134, 2275, 2540, 2686
Alw21I GWGCWC 3 cut(s) 959, 1404, 2823
Alw26I GTCTC 5 cut(s) 36, 166, 1025, 1340, 1478
Alw44I GTGCAC 1 cut(s) 1400
AlwI GGATC 4 cut(s) 1585, 2125, 3353, 3479
AlwNI CAGNNNCTG 4 cut(s) 1370, 1748, 3761, 3832
Ama87I CYCGRG 2 cut(s) 3047, 3618
Aor51HI AGCGCT 1 cut(s) 3197
AoxI GGCC 6 cut(s) 11, 1428, 2345, 2846, 2908, 2944
ApaLI GTGCAC 1 cut(s) 1400
ApeKI GCWGC 7 cut(s) 1048, 1289, 2468, 3091, 3454, 3571, 3782
ApoI RAATTY 8 cut(s) 1112, 1391, 2180, 2208, 2326, 2681, 3111, 3890
ArsI GACNNNNNNTTYG 1 cut(s) 3875
AseI ATTAAT 1 cut(s) 843
AspLEI GCGC 3 cut(s) 2778, 3198, 3459
AspS9I GGNCC 5 cut(s) 126, 1452, 2856, 2908, 3379
AsuC2I CCSGG 3 cut(s) 1857, 3048, 3049
AsuHPI GGTGA 7 cut(s) 103, 167, 676, 752, 2261, 2854, 3242
AsuII TTCGAA 1 cut(s) 2178
AvaI CYCGRG 2 cut(s) 3047, 3618
AvaII GGWCC 4 cut(s) 126, 1452, 2856, 3379
BaeGI GKGCMC 2 cut(s) 1404, 2715
BaeI ACNNNNGTAYC 4 cut(s) 768, 801, 1851, 1884
BanI GGYRCC 1 cut(s) 3023
BanII GRGCYC 1 cut(s) 2823
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BbsI GAAGAC 3 cut(s) 2040, 2294, 3222
Bbv12I GWGCWC 3 cut(s) 959, 1404, 2823
BbvI GCAGC 7 cut(s) 1060, 1276, 2480, 3103, 3466, 3558, 3769
BceAI ACGGC 1 cut(s) 1248
BcgI CGANNNNNNTGC 2 cut(s) 2702, 2736
BciT130I CCWGG 6 cut(s) 134, 212, 2136, 2277, 2542, 2688
BciVI GTATCC 2 cut(s) 1320, 3745
BclI TGATCA 2 cut(s) 196, 877
BcnI CCSGG 3 cut(s) 1857, 3048, 3049
BcoDI GTCTC 5 cut(s) 36, 166, 1025, 1340, 1478
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 5 cut(s) 21, 168, 431, 1760, 2235
BfmI CTRYAG 2 cut(s) 588, 1119
BfoI RGCGCY 2 cut(s) 3199, 3460
BfuAI ACCTGC 2 cut(s) 2879, 3390
BfuI GTATCC 2 cut(s) 1320, 3745
BisI GCNGC 8 cut(s) 1049, 1290, 2469, 3092, 3455, 3572, 3605, 3783
BlpI GCTNAGC 2 cut(s) 1044, 2655
BlsI GCNGC 8 cut(s) 1050, 1291, 2470, 3093, 3456, 3573, 3606, 3784
Bme1390I CCNGG 9 cut(s) 134, 212, 1857, 2136, 2277, 2542, 2688, 3048, 3049
Bme18I GGWCC 4 cut(s) 126, 1452, 2856, 3379
BmeRI GACNNNNNGTC 1 cut(s) 2041
BmeT110I CYCGRG 2 cut(s) 3047, 3618
BmgT120I GGNCC 5 cut(s) 126, 1452, 2856, 2908, 3379
BmiI GGNNCC 5 cut(s) 1813, 2909, 3025, 3380, 3590
BmrFI CCNGG 9 cut(s) 134, 212, 1857, 2136, 2277, 2542, 2688, 3048, 3049
BmrI ACTGGG 2 cut(s) 2292, 3244
BmsI GCATC 8 cut(s) 1081, 1297, 1966, 2089, 2246, 2568, 3436, 3754
BmuI ACTGGG 2 cut(s) 2292, 3244
BpiI GAAGAC 3 cut(s) 2040, 2294, 3222
Bpu10I CCTNAGC 1 cut(s) 1155
Bpu1102I GCTNAGC 2 cut(s) 1044, 2655
Bpu14I TTCGAA 1 cut(s) 2178
BpuEI CTTGAG 2 cut(s) 88, 3230
BpuMI CCSGG 3 cut(s) 1857, 3048, 3049
BsaAI YACGTR 1 cut(s) 2366
BsaJI CCNNGG 6 cut(s) 210, 2134, 2276, 2541, 2875, 3047
Bsc4I CCNNNNNNNGG 7 cut(s) 168, 592, 1646, 2282, 3054, 3272, 3784
Bse1I ACTGG 6 cut(s) 162, 1833, 2287, 2314, 2455, 3239
Bse3DI GCAATG 4 cut(s) 1585, 2731, 2938, 3362
BseBI CCWGG 6 cut(s) 134, 212, 2136, 2277, 2542, 2688
BseDI CCNNGG 6 cut(s) 210, 2134, 2276, 2541, 2875, 3047
BseLI CCNNNNNNNGG 7 cut(s) 168, 592, 1646, 2282, 3054, 3272, 3784
BseMI GCAATG 4 cut(s) 1585, 2731, 2938, 3362
BseMII CTCAG 8 cut(s) 110, 1053, 1740, 1860, 1886, 2646, 3075, 3407
BseNI ACTGG 6 cut(s) 162, 1833, 2287, 2314, 2455, 3239
BseSI GKGCMC 2 cut(s) 1404, 2715
BseXI GCAGC 7 cut(s) 1060, 1276, 2480, 3103, 3466, 3558, 3769
BseYI CCCAGC 3 cut(s) 2012, 2936, 3778
BsgI GTGCAG 1 cut(s) 1713
Bsh1236I CGCG 1 cut(s) 3791
Bsh1285I CGRYCG 1 cut(s) 3789
BshFI GGCC 6 cut(s) 13, 1430, 2347, 2848, 2910, 2946
BshNI GGYRCC 1 cut(s) 3023
BsiEI CGRYCG 1 cut(s) 3789
BsiHKAI GWGCWC 3 cut(s) 959, 1404, 2823
BsiHKCI CYCGRG 2 cut(s) 3047, 3618
BsiSI CCGG 2 cut(s) 1857, 3048
BslFI GGGAC 5 cut(s) 1835, 2138, 2293, 3392, 3648
BslI CCNNNNNNNGG 7 cut(s) 168, 592, 1646, 2282, 3054, 3272, 3784
BsmAI GTCTC 5 cut(s) 36, 166, 1025, 1340, 1478
BsmBI CGTCTC 1 cut(s) 1340
BsmFI GGGAC 5 cut(s) 1835, 2138, 2293, 3392, 3648
BsmI GAATGC 3 cut(s) 1138, 2752, 2778
BsnI GGCC 6 cut(s) 13, 1430, 2347, 2848, 2910, 2946
BsoBI CYCGRG 2 cut(s) 3047, 3618
Bsp119I TTCGAA 1 cut(s) 2178
Bsp1286I GDGCHC 4 cut(s) 959, 1404, 2715, 2823
Bsp1720I GCTNAGC 2 cut(s) 1044, 2655
BspACI CCGC 7 cut(s) 290, 1356, 2247, 2836, 3604, 3785, 3791
BspANI GGCC 6 cut(s) 13, 1430, 2347, 2848, 2910, 2946
BspCNI CTCAG 8 cut(s) 111, 1054, 1741, 1859, 1885, 2647, 3074, 3406
BspFNI CGCG 1 cut(s) 3791
BspHI TCATGA 2 cut(s) 766, 880
BspLI GGNNCC 5 cut(s) 1813, 2909, 3025, 3380, 3590
BspMI ACCTGC 2 cut(s) 2879, 3390
BspPI GGATC 4 cut(s) 1585, 2125, 3353, 3479
BspQI GCTCTTC 1 cut(s) 3374
BspT104I TTCGAA 1 cut(s) 2178
BspT107I GGYRCC 1 cut(s) 3023
BsrBI CCGCTC 1 cut(s) 3604
BsrDI GCAATG 4 cut(s) 1585, 2731, 2938, 3362
BsrI ACTGG 6 cut(s) 162, 1833, 2287, 2314, 2455, 3239
BssECI CCNNGG 6 cut(s) 210, 2134, 2276, 2541, 2875, 3047
BssT1I CCWWGG 1 cut(s) 2875
Bst2UI CCWGG 6 cut(s) 134, 212, 2136, 2277, 2542, 2688
Bst4CI ACNGT 6 cut(s) 86, 179, 566, 2119, 2583, 2833
BstAPI GCANNNNNTGC 1 cut(s) 3290
BstBAI YACGTR 1 cut(s) 2366
BstBI TTCGAA 1 cut(s) 2178
BstC8I GCNNGC 6 cut(s) 688, 1411, 2345, 2473, 2973, 3609
BstEII GGTNACC 1 cut(s) 931
BstFNI CGCG 1 cut(s) 3791
BstH2I RGCGCY 2 cut(s) 3199, 3460
BstHHI GCGC 3 cut(s) 2778, 3198, 3459
BstMAI GTCTC 5 cut(s) 36, 166, 1025, 1340, 1478
BstMCI CGRYCG 1 cut(s) 3789
BstMWI GCNNNNNNNGC 7 cut(s) 1001, 1100, 1944, 2928, 3040, 3290, 3788
BstNI CCWGG 6 cut(s) 134, 212, 2136, 2277, 2542, 2688
BstNSI RCATGY 2 cut(s) 2273, 3014
BstPI GGTNACC 1 cut(s) 931
BstSCI CCNGG 9 cut(s) 132, 210, 1855, 2134, 2275, 2540, 2686, 3046, 3047
BstSFI CTRYAG 2 cut(s) 588, 1119
BstSLI GKGCMC 2 cut(s) 1404, 2715
BstUI CGCG 1 cut(s) 3791
BstV1I GCAGC 7 cut(s) 1060, 1276, 2480, 3103, 3466, 3558, 3769
BstV2I GAAGAC 3 cut(s) 2040, 2294, 3222
BsuI GTATCC 2 cut(s) 1320, 3745
BsuRI GGCC 6 cut(s) 13, 1430, 2347, 2848, 2910, 2946
BtsIMutI CAGTG 7 cut(s) 91, 1815, 2069, 2448, 2579, 3356, 3407
BveI ACCTGC 2 cut(s) 2879, 3390
Cac8I GCNNGC 6 cut(s) 688, 1411, 2345, 2473, 2973, 3609
CaiI CAGNNNCTG 4 cut(s) 1370, 1748, 3761, 3832
CciI TCATGA 2 cut(s) 766, 880
CfoI GCGC 3 cut(s) 2778, 3198, 3459
Cfr13I GGNCC 5 cut(s) 126, 1452, 2856, 2908, 3379
Cfr9I CCCGGG 1 cut(s) 3047
CseI GACGC 1 cut(s) 1357
Csp6I GTAC 9 cut(s) 428, 942, 1860, 1979, 2155, 2320, 2367, 2793, 3691
CviQI GTAC 9 cut(s) 428, 942, 1860, 1979, 2155, 2320, 2367, 2793, 3691
DraI TTTAAA 1 cut(s) 2757
DraIII CACNNNGTG 1 cut(s) 2121
DriI GACNNNNNGTC 1 cut(s) 2041
Eam1105I GACNNNNNGTC 1 cut(s) 2041
Ecl136II GAGCTC 1 cut(s) 2821
Eco130I CCWWGG 1 cut(s) 2875
Eco147I AGGCCT 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 2823
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 4 cut(s) 126, 1452, 2856, 3379
Eco47III AGCGCT 1 cut(s) 3197
Eco53kI GAGCTC 1 cut(s) 2821
Eco57I CTGAAG 6 cut(s) 1347, 1821, 2045, 2130, 3288, 3854
Eco88I CYCGRG 2 cut(s) 3047, 3618
Eco91I GGTNACC 1 cut(s) 931
EcoICRI GAGCTC 1 cut(s) 2821
EcoO109I RGGNCCY 2 cut(s) 1452, 3379
EcoO65I GGTNACC 1 cut(s) 931
EcoRI GAATTC 2 cut(s) 2208, 3111
EcoRII CCWGG 6 cut(s) 132, 210, 2134, 2275, 2540, 2686
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 2875
EcoT22I ATGCAT 1 cut(s) 2261
EcoT38I GRGCYC 1 cut(s) 2823
ErhI CCWWGG 1 cut(s) 2875
Esp3I CGTCTC 1 cut(s) 1340
FalI AAGNNNNNCTT 4 cut(s) 801, 833, 3027, 3059
FaqI GGGAC 5 cut(s) 1835, 2138, 2293, 3392, 3648
FauNDI CATATG 4 cut(s) 150, 1126, 1489, 1934
FbaI TGATCA 2 cut(s) 196, 877
Fnu4HI GCNGC 8 cut(s) 1049, 1290, 2469, 3092, 3455, 3572, 3605, 3783
FriOI GRGCYC 1 cut(s) 2823
Fsp4HI GCNGC 8 cut(s) 1049, 1290, 2469, 3092, 3455, 3572, 3605, 3783
FspBI CTAG 5 cut(s) 21, 168, 431, 1760, 2235
GlaI GCGC 3 cut(s) 2777, 3197, 3458
GluI GCNGC 8 cut(s) 1049, 1290, 2469, 3092, 3455, 3572, 3605, 3783
GsaI CCCAGC 3 cut(s) 2016, 2940, 3782
HaeII RGCGCY 2 cut(s) 3199, 3460
HaeIII GGCC 6 cut(s) 13, 1430, 2347, 2848, 2910, 2946
HapII CCGG 2 cut(s) 1857, 3048
HgaI GACGC 1 cut(s) 1357
HhaI GCGC 3 cut(s) 2778, 3198, 3459
Hin6I GCGC 3 cut(s) 2776, 3196, 3457
HinP1I GCGC 3 cut(s) 2776, 3196, 3457
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 3 cut(s) 2645, 3041, 3870
HpaII CCGG 2 cut(s) 1857, 3048
HphI GGTGA 7 cut(s) 103, 167, 676, 752, 2261, 2854, 3242
Hpy166II GTNNAC 3 cut(s) 508, 1402, 3016
Hpy8I GTNNAC 3 cut(s) 508, 1402, 3016
HpyCH4III ACNGT 6 cut(s) 86, 179, 566, 2119, 2583, 2833
HpyCH4IV ACGT 1 cut(s) 2365
HpyF10VI GCNNNNNNNGC 7 cut(s) 1001, 1100, 1944, 2928, 3040, 3290, 3788
HpySE526I ACGT 1 cut(s) 2365
HspAI GCGC 3 cut(s) 2776, 3196, 3457
Ksp22I TGATCA 2 cut(s) 196, 877
LguI GCTCTTC 1 cut(s) 3374
LmnI GCTCC 7 cut(s) 1678, 2278, 2365, 2600, 2818, 2879, 3554
Lsp1109I GCAGC 7 cut(s) 1060, 1276, 2480, 3103, 3466, 3558, 3769
LweI GCATC 8 cut(s) 1081, 1297, 1966, 2089, 2246, 2568, 3436, 3754
MaeI CTAG 5 cut(s) 21, 168, 431, 1760, 2235
MaeII ACGT 1 cut(s) 2365
MbiI CCGCTC 1 cut(s) 3604
MfeI CAATTG 3 cut(s) 1236, 1254, 2080
MhlI GDGCHC 4 cut(s) 959, 1404, 2715, 2823
MlyI GAGTC 6 cut(s) 103, 167, 1838, 1923, 2200, 2284
MmeI TCCRAC 2 cut(s) 153, 3532
Mph1103I ATGCAT 1 cut(s) 2261
MslI CAYNNNNRTG 3 cut(s) 153, 1356, 2166
MspA1I CMGCKG 1 cut(s) 3782
MspI CCGG 2 cut(s) 1857, 3048
MspR9I CCNGG 9 cut(s) 134, 212, 1857, 2136, 2277, 2542, 2688, 3048, 3049
MunI CAATTG 3 cut(s) 1236, 1254, 2080
Mva1269I GAATGC 3 cut(s) 1138, 2752, 2778
MvaI CCWGG 6 cut(s) 134, 212, 2136, 2277, 2542, 2688
MvnI CGCG 1 cut(s) 3791
MwoI GCNNNNNNNGC 7 cut(s) 1001, 1100, 1944, 2928, 3040, 3290, 3788
NciI CCSGG 3 cut(s) 1857, 3048, 3049
NdeI CATATG 4 cut(s) 150, 1126, 1489, 1934
NlaIV GGNNCC 5 cut(s) 1813, 2909, 3025, 3380, 3590
NmuCI GTSAC 6 cut(s) 569, 664, 1207, 2192, 3230, 3314
NsiI ATGCAT 1 cut(s) 2261
NspI RCATGY 2 cut(s) 2273, 3014
NspV TTCGAA 1 cut(s) 2178
PagI TCATGA 2 cut(s) 766, 880
PceI AGGCCT 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 3374
PctI GAATGC 3 cut(s) 1138, 2752, 2778
PflFI GACNNNGTC 2 cut(s) 1787, 1847
PkrI GCNGC 8 cut(s) 1050, 1291, 2470, 3093, 3456, 3573, 3606, 3784
PleI GAGTC 6 cut(s) 102, 166, 1838, 1923, 2199, 2284
PpsI GAGTC 6 cut(s) 102, 166, 1838, 1923, 2199, 2284
Ppu21I YACGTR 1 cut(s) 2366
PpuMI RGGWCCY 2 cut(s) 1452, 3379
PshBI ATTAAT 1 cut(s) 843
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 2823
Psp5II RGGWCCY 2 cut(s) 1452, 3379
Psp6I CCWGG 6 cut(s) 132, 210, 2134, 2275, 2540, 2686
PspEI GGTNACC 1 cut(s) 931
PspFI CCCAGC 3 cut(s) 2012, 2936, 3778
PspGI CCWGG 6 cut(s) 132, 210, 2134, 2275, 2540, 2686
PspN4I GGNNCC 5 cut(s) 1813, 2909, 3025, 3380, 3590
PspPI GGNCC 5 cut(s) 126, 1452, 2856, 2908, 3379
PspPPI RGGWCCY 2 cut(s) 1452, 3379
PstNI CAGNNNCTG 4 cut(s) 1370, 1748, 3761, 3832
PsyI GACNNNGTC 2 cut(s) 1787, 1847
PvuII CAGCTG 1 cut(s) 3782
RsaI GTAC 9 cut(s) 429, 943, 1861, 1980, 2156, 2321, 2368, 2794, 3692
RsaNI GTAC 9 cut(s) 428, 942, 1860, 1979, 2155, 2320, 2367, 2793, 3691
RseI CAYNNNNRTG 3 cut(s) 153, 1356, 2166
SacI GAGCTC 1 cut(s) 2823
SapI GCTCTTC 1 cut(s) 3374
SatI GCNGC 8 cut(s) 1049, 1290, 2469, 3092, 3455, 3572, 3605, 3783
Sau96I GGNCC 5 cut(s) 126, 1452, 2856, 2908, 3379
SchI GAGTC 6 cut(s) 103, 167, 1838, 1923, 2200, 2284
ScrFI CCNGG 9 cut(s) 134, 212, 1857, 2136, 2277, 2542, 2688, 3048, 3049
SduI GDGCHC 4 cut(s) 959, 1404, 2715, 2823
SfaNI GCATC 8 cut(s) 1081, 1297, 1966, 2089, 2246, 2568, 3436, 3754
SfcI CTRYAG 2 cut(s) 588, 1119
SfuI TTCGAA 1 cut(s) 2178
SinI GGWCC 4 cut(s) 126, 1452, 2856, 3379
SmaI CCCGGG 1 cut(s) 3049
SmiMI CAYNNNNRTG 3 cut(s) 153, 1356, 2166
SmlI CTYRAG 2 cut(s) 103, 3209
SmoI CTYRAG 2 cut(s) 103, 3209
SpeI ACTAGT 1 cut(s) 430
SseBI AGGCCT 1 cut(s) 13
SsiI CCGC 7 cut(s) 290, 1356, 2247, 2836, 3604, 3785, 3791
SspMI CTAG 5 cut(s) 21, 168, 431, 1760, 2235
SstI GAGCTC 1 cut(s) 2823
StuI AGGCCT 1 cut(s) 13
StyD4I CCNGG 9 cut(s) 132, 210, 1855, 2134, 2275, 2540, 2686, 3046, 3047
StyI CCWWGG 1 cut(s) 2875
TaaI ACNGT 6 cut(s) 86, 179, 566, 2119, 2583, 2833
TaiI ACGT 1 cut(s) 2368
TaqI TCGA 6 cut(s) 35, 396, 1338, 1604, 1993, 2178
TatI WGTACW 1 cut(s) 3690
TauI GCSGC 1 cut(s) 3607
TscAI CASTG 7 cut(s) 91, 1822, 2069, 2455, 2586, 3356, 3407
TseFI GTSAC 6 cut(s) 569, 664, 1207, 2192, 3230, 3314
TseI GCWGC 7 cut(s) 1048, 1289, 2468, 3091, 3454, 3571, 3782
Tsp45I GTSAC 6 cut(s) 569, 664, 1207, 2192, 3230, 3314
TspGWI ACGGA 2 cut(s) 683, 2914
TspMI CCCGGG 1 cut(s) 3047
TspRI CASTG 7 cut(s) 91, 1822, 2069, 2455, 2586, 3356, 3407
Tth111I GACNNNGTC 2 cut(s) 1787, 1847
VneI GTGCAC 1 cut(s) 1400
VpaK11BI GGWCC 4 cut(s) 126, 1452, 2856, 3379
VspI ATTAAT 1 cut(s) 843
XapI RAATTY 8 cut(s) 1112, 1391, 2180, 2208, 2326, 2681, 3111, 3890
XceI RCATGY 2 cut(s) 2273, 3014
XmaI CCCGGG 1 cut(s) 3047
XspI CTAG 5 cut(s) 21, 168, 431, 1760, 2235
Zsp2I ATGCAT 1 cut(s) 2261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.