Rroxscaffold_3G00251440

SNF2 domain-containing protein CLASSY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001072
Physical Location & Seq
Forward (+)
45318948 .. 45323473
4526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251440.1

Sequence Viewer

Length: 3222 bp
ATGTTTGACTTTAGCTTGCCTATAGCTAGTAGAACAAGGAAGGGGCAGGAGAGAGTTTACAAGGAGTTCAATGAGAAAAAGAAGCAGAAGAAGAAGAATGAGGGTGGCTCGGCGCCCATTGATTTTGAGAAATTGGGGTCTGAATGCATACTTCTTTCGGATGATAGCATGGAGGATGTCGATTCAGACGGTTCTGAGTTAGAAAAGAGTGCTGCAAAGGGAAGTGAGAAGGTGGGGAAAGGGGGAGTGTGGAGGTTGGGGGTGTTGAGACAAGTACAAGCCGATTCTGTAGCTGATTTGGATGATGGTGACAGTGAAGGTGTGAAAGTCATTAGTTTGGTTAGTGATAGTGATGATGAGGTGGTATCAATAAGTGAAGAGGGAGGTGGAGGAGACTCTGACGGTGATAAGGATAGTGATTCGAGAAGTGTAGGTTCAGACGATGAACACAATGTGTACTCGGCTGATGATGATGATGAAGATGATATTGAAAGCATCTCTTCAGATGATTGTGCAAGTGATTATTCGAATAATGAGGGACCGGTTAATTTAGATTGTGAAACTGAGGATGCAGAGAGAAGTGGGGGAGAGGGAGAAAAGGTTGGTATTTGTCAAAAGAGAAACAGAAACATTGGAGAGGATGGTCAAAAGTTCAATTGTGTAGCACACCGTACTCGGTCACGGTCTACATCTCGTTCTTCTGCCAATGATGATCAGAGGGATGATGTTGATGACAATCACTGCAGTAGTCAGACAAATAGGCGTCGTAAAATGAATTCAAAGTGCAACAATAGAAGAAGTGGTGACAATGATGAGACCTATGCTTGCAGTCTGAACAATGGTCGTGATAGATGGAATGATGTGCAGAAAGCATCTCAAAAGAATGAAGAGCTGAGGAAGCTTACTAAGAAAAAGTGCACCAGGGGAAAAAAGGATGCCAGCGCTTACAAAATTCTTGCAGATTCGATTTATGAGGATGGAGAATTGCCCCCAGAAGGGTCAGCTTCCTTTGGGGATAAAGCCGTTGAAAATGGAACTCTTCCGTTGAAGTTCTCATTTGGTGAGCCGCCAAGTATTGCTCCAGTAGAGAAGTCAGATTGTGAGAAAGAACTGGAGAACCTTTTTAAAGAAATGGAATTTGCTATTAGATCTGCTCAGATTGGTTCTACTAACTATAGTGAGGTTGAAATTAATGACACAGTCCCTCCAGTTAAGGAAGTTACTCAGCATATGCTTTGTAGTCAAGGGAAACATCATCTTATACTTGATGAAGAAATTGGACTGATTTGCAAATTTTGCCCTCATGTGCATCAGGAGATCAAGTATATTGTACCAGAATTTGCTCCAAACCCTTATGGACGATCTGGCAGGAGATTTTATGAAGAAGACAACCGGTCTATTCCTGTTGAGCTACAATGTCATGACTCTGGGTCTGGCTTTAATTCCCATGCTCATGTTGATGGCACGGTATGGGACTTAATTCCTGGTGTTAAAAGTAGTGTGTACCCTCATCAGCGTGCAGGTTTTGAATTTATTTGGAGTCATATAGCCGGTGGAATTCATCTTGAAAAGTTGCAGAAGACGAGTAGTGCTGATGGTGGGGGTGGATGCATAATATCACATGCACCTGGAACAGGGAAGACACGTCTGACCATTGTCTTTCTTCAGGCATACATGAGATTTAACCCAAAATGCAGGCCGCTTATAATCGCTCCTCGTACCATGCTTCTCACCTGGGAAGAGGAGTTTAAAAAATGGGGCTTCGATGTACCCTTTCACAATCTCAACAATCAGAGTTTATCCGGGAAGGAGAATAAAGCAGCTCTCAAGATTTTGGTGCAAGATGGAGGCCAGCGCCATGTGAAAAGTGTGGATGGCAGTCGTTGGTTGAAGTTATATTCCTGGAAAAAGGAAAGAAGCATCCTTGGAATTAGTTACAGACTTTTTGAGAAACTTTCTACTGCACATAATTCTGGAGGCAAGAAAGCTGACCTAGCTAAGACAATGAGGAATATTCTATTAGACTTTCCTGGTATTGTAGTCTTTGATGAAGGGCATACACCTCGAAATGATAAGAGTCATATGTGGAAGGCTTCATCAAATATCAAAACACATAGACGTATCATTCTCTCAGGAACTCCTTTTCAAAACAATTTTGATGAGCTGTACAATACAATTTGCTTGGCAAGGCCAGACTGGATCAGGCCTACATGCAGTGGACGAAGCCGAAAAAGAAATTTAGGGAAAGCGCAACAGACGTCTCTGGCCAAATACATTGCCAAAGGTGCCGGTGATAAAGCAAAACAAGCTGAGGAAATAGGAGCTAGAATTGCCCCAATTGTGCATGTTTACAGAGGTTCCATACTACGAGATACACTTCCCGGTTTGAGGAACTCGATTGTTGTTTTACAGCCAAGCAAGTTACAGAAAGAAATTTCTACAAGAATTCAAGAGAGGAAGGATCTTCCATTCTTTAAAGATGAACATTACGAGACTTTAATATCTGTTCATCCTTCGGCTTTGCTGAACCTGGAGAAGGAAAAGAGGGTATCCTTGCTTAATCAGGTCGACTTGGAGAGGTTAAAGGAAAAAAGATTAAATCCTGAATCTGGGATGAAGGCAAAGTTTGTGATTGAACTCGTACGGCTTAGTGATGCTATGAATGAAAGAGTTCTGGTGTTCAGTCAGTATATTAATGCCTTGACCTTTTTAAGGGAGCTTTTGAAATCTCAGTTTCAATGGACTGAAGGGGAAGAGATTCTTTATATGTATGGGGCAGATGATGAAAAGCAGCGCCAAACATCAATGAAGGCTTTCAATGATCCATCAAGCAGAGCCAAGGTGTTATTAGCCTCAACGAAAGGTTGCAACGAAGGAATAAATTTAGTTGGAGCTTCAAGGGTTGTGCTGCTTGATGTTACATGGAACCCATCTGTCGAGAGGCAAGCTATATCCCGAGCATACCGATTGGGACAGAAGAAAATTGTGTTCATCTACCATCTTCTCATTGCTGGGGCAAGAGAAGAGGACAAACACAGTAGGCAAGTTGAGAAGCATCATCTGTCTGAATTGGTATTTTGTTGTTCGGATACGGAAGCTGCCAAGAAAATCCCAGCTGCAGTTGCAGAGGACAAGATTTTGGAAGAGATGGTTCAGCATGAGAAACTCAAGCATATATTTGAAAAGATAACATTACTGGATGAAGCCTCATGTTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1073

Amino Acids

120.38

Weight (kDa)

6.21

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 522 - 715 3.2e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 533 - 840 1.3e-21 SNF2-related domain
Helicase_C PF00271 873 - 991 8.5e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1706
AatII GACGTC 1 cut(s) 2261
Acc36I ACCTGC 1 cut(s) 1511
AccB1I GGYRCC 2 cut(s) 112, 2285
AccI GTMKAC 2 cut(s) 686, 2568
AciI CCGC 2 cut(s) 1067, 1700
AclWI GGATC 3 cut(s) 2207, 2469, 2816
AcoI YGGCCR 1 cut(s) 2265
AcuI CTGAAG 3 cut(s) 486, 1649, 2766
AcyI GRCGYC 3 cut(s) 113, 763, 2258
AdeI CACNNNGTG 1 cut(s) 454
AfaI GTAC 9 cut(s) 276, 458, 673, 1332, 1505, 1720, 1770, 2168, 2643
AfeI AGCGCT 1 cut(s) 943
AfiI CCNNNNNNNGG 7 cut(s) 995, 996, 1634, 2388, 2536, 2609, 2712
AflIII ACRYGT 1 cut(s) 1643
AgeI ACCGGT 2 cut(s) 541, 1392
AhdI GACNNNNNGTC 2 cut(s) 1393, 1429
AjiI CACGTC 1 cut(s) 1646
AjnI CCWGG 7 cut(s) 920, 1483, 1627, 1733, 1901, 2029, 2529
Alw21I GWGCWC 1 cut(s) 920
Alw26I GTCTC 5 cut(s) 262, 387, 809, 2265, 2486
Alw44I GTGCAC 1 cut(s) 916
AlwI GGATC 3 cut(s) 2207, 2469, 2816
Ama87I CYCGRG 1 cut(s) 2955
Aor51HI AGCGCT 1 cut(s) 943
AoxI GGCC 5 cut(s) 1697, 1849, 2189, 2204, 2265
ApaLI GTGCAC 1 cut(s) 916
ApeKI GCWGC 6 cut(s) 212, 1820, 2791, 2908, 3098, 3116
AseI ATTAAT 2 cut(s) 1191, 2694
AsiGI ACCGGT 2 cut(s) 541, 1392
Asp700I GAANNNNTTC 3 cut(s) 2670, 2757, 2813
AspLEI GCGC 5 cut(s) 115, 944, 1857, 2251, 2796
AspS9I GGNCC 1 cut(s) 539
AsuC2I CCSGG 2 cut(s) 1804, 2382
AsuHPI GGTGA 6 cut(s) 320, 416, 815, 1073, 1723, 2303
AsuII TTCGAA 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 2955
AvaII GGWCC 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 920
BalI TGGCCA 1 cut(s) 2267
BanI GGYRCC 2 cut(s) 112, 2285
BbsI GAAGAC 3 cut(s) 1392, 1586, 1646
Bbv12I GWGCWC 1 cut(s) 920
BbvCI CCTCAGC 2 cut(s) 893, 2310
BbvI GCAGC 6 cut(s) 199, 1832, 2803, 2895, 3085, 3103
BceAI ACGGC 2 cut(s) 1007, 2660
BcgI CGANNNNNNTGC 2 cut(s) 2045, 2079
BciT130I CCWGG 7 cut(s) 922, 1485, 1629, 1735, 1903, 2031, 2531
BciVI GTATCC 2 cut(s) 2560, 3082
BclI TGATCA 1 cut(s) 712
BcnI CCSGG 2 cut(s) 1804, 2382
BcoDI GTCTC 5 cut(s) 262, 387, 809, 2265, 2486
BfaI CTAG 3 cut(s) 27, 1994, 2325
BfmI CTRYAG 5 cut(s) 21, 288, 742, 1174, 3117
BfoI RGCGCY 4 cut(s) 116, 945, 1858, 2797
BfuAI ACCTGC 1 cut(s) 1511
BfuI GTATCC 2 cut(s) 2560, 3082
BglII AGATCT 1 cut(s) 1148
BisI GCNGC 8 cut(s) 213, 1067, 1700, 1821, 2792, 2909, 3099, 3117
BlsI GCNGC 8 cut(s) 214, 1068, 1701, 1822, 2793, 2910, 3100, 3118
Bme1390I CCNGG 9 cut(s) 922, 1485, 1629, 1735, 1804, 1903, 2031, 2382, 2531
Bme18I GGWCC 1 cut(s) 539
BmeRI GACNNNNNGTC 2 cut(s) 1393, 1429
BmeT110I CYCGRG 1 cut(s) 2955
BmgBI CACGTC 1 cut(s) 1646
BmgT120I GGNCC 1 cut(s) 539
BmiI GGNNCC 5 cut(s) 114, 540, 2287, 2359, 2927
BmrFI CCNGG 9 cut(s) 922, 1485, 1629, 1735, 1804, 1903, 2031, 2382, 2531
BmsI GCATC 9 cut(s) 504, 559, 881, 925, 1318, 1598, 1929, 2644, 3064
BoxI GACNNNNGTC 1 cut(s) 1655
BpiI GAAGAC 3 cut(s) 1392, 1586, 1646
BplI GAGNNNNNCTC 2 cut(s) 92, 124
BpmI CTGGAG 5 cut(s) 1065, 1133, 1191, 1995, 2552
Bpu10I CCTNAGC 2 cut(s) 893, 2310
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 2 cut(s) 1811, 3152
BpuMI CCSGG 2 cut(s) 1804, 2382
BsaBI GATNNNNATC 1 cut(s) 735
BsaHI GRCGYC 3 cut(s) 113, 763, 2258
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 4 cut(s) 921, 1734, 1924, 2838
BsaWI WCCGGW 2 cut(s) 541, 1392
BsaXI ACNNNNNCTCC 6 cut(s) 585, 615, 1189, 1219, 1307, 1337
Bsc4I CCNNNNNNNGG 7 cut(s) 995, 996, 1634, 2388, 2536, 2609, 2712
Bse118I RCCGGY 4 cut(s) 541, 1392, 1550, 2288
Bse1I ACTGG 5 cut(s) 1082, 1116, 1208, 2201, 3201
Bse3DI GCAATG 2 cut(s) 2274, 3006
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 7 cut(s) 922, 1485, 1629, 1735, 1903, 2031, 2531
BseDI CCNNGG 4 cut(s) 921, 1734, 1924, 2838
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 7 cut(s) 995, 996, 1634, 2388, 2536, 2609, 2712
BseMI GCAATG 2 cut(s) 2274, 3006
BseMII CTCAG 8 cut(s) 186, 555, 884, 1169, 1238, 2145, 2301, 2744
BseNI ACTGG 5 cut(s) 1082, 1116, 1208, 2201, 3201
BseRI GAGGAG 3 cut(s) 405, 1704, 1757
BseSI GKGCMC 1 cut(s) 920
BseXI GCAGC 6 cut(s) 199, 1832, 2803, 2895, 3085, 3103
BseYI CCCAGC 2 cut(s) 3011, 3112
BsgI GTGCAG 3 cut(s) 884, 1539, 1947
BshFI GGCC 5 cut(s) 1699, 1851, 2191, 2206, 2267
BshNI GGYRCC 2 cut(s) 112, 2285
BshTI ACCGGT 2 cut(s) 541, 1392
BsiHKAI GWGCWC 1 cut(s) 920
BsiHKCI CYCGRG 1 cut(s) 2955
BsiSI CCGG 6 cut(s) 542, 1393, 1551, 1803, 2289, 2382
BsiWI CGTACG 1 cut(s) 2641
BslFI GGGAC 4 cut(s) 552, 1187, 1487, 2985
BslI CCNNNNNNNGG 7 cut(s) 995, 996, 1634, 2388, 2536, 2609, 2712
BsmAI GTCTC 5 cut(s) 262, 387, 809, 2265, 2486
BsmBI CGTCTC 1 cut(s) 2265
BsmFI GGGAC 4 cut(s) 552, 1187, 1487, 2985
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 5 cut(s) 1699, 1851, 2191, 2206, 2267
Bso31I GGTCTC 1 cut(s) 809
BsoBI CYCGRG 1 cut(s) 2955
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 1 cut(s) 920
Bsp1407I TGTACA 1 cut(s) 2166
Bsp143I GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
BspACI CCGC 2 cut(s) 1067, 1700
BspANI GGCC 5 cut(s) 1699, 1851, 2191, 2206, 2267
BspCNI CTCAG 8 cut(s) 187, 556, 885, 1168, 1237, 2144, 2302, 2743
BspHI TCATGA 1 cut(s) 1420
BspLI GGNNCC 5 cut(s) 114, 540, 2287, 2359, 2927
BspMAI CTGCAG 2 cut(s) 746, 3121
BspMI ACCTGC 1 cut(s) 1511
BspPI GGATC 3 cut(s) 2207, 2469, 2816
BspQI GCTCTTC 1 cut(s) 882
BspT104I TTCGAA 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 112, 2285
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 2 cut(s) 2274, 3006
BsrFI RCCGGY 4 cut(s) 541, 1392, 1550, 2288
BsrGI TGTACA 1 cut(s) 2166
BsrI ACTGG 5 cut(s) 1082, 1116, 1208, 2201, 3201
BssAI RCCGGY 4 cut(s) 541, 1392, 1550, 2288
BssECI CCNNGG 4 cut(s) 921, 1734, 1924, 2838
BssMI GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
BssNI GRCGYC 3 cut(s) 113, 763, 2258
BssT1I CCWWGG 2 cut(s) 1924, 2838
Bst2UI CCWGG 7 cut(s) 922, 1485, 1629, 1735, 1903, 2031, 2531
Bst4CI ACNGT 8 cut(s) 191, 314, 404, 671, 684, 1201, 1468, 3038
Bst6I CTCTTC 8 cut(s) 372, 505, 882, 1044, 1734, 2748, 3018, 3138
BstACI GRCGYC 3 cut(s) 113, 763, 2258
BstAPI GCANNNNNTGC 1 cut(s) 1296
BstAUI TGTACA 1 cut(s) 2166
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 7 cut(s) 17, 826, 940, 1518, 1697, 1853, 2946
BstENI CCTNNNNNAGG 3 cut(s) 1632, 2534, 2710
BstH2I RGCGCY 4 cut(s) 116, 945, 1858, 2797
BstHHI GCGC 5 cut(s) 115, 944, 1857, 2251, 2796
BstKTI GATC 7 cut(s) 715, 1151, 1320, 1364, 2202, 2464, 2824
BstMAI GTCTC 5 cut(s) 262, 387, 809, 2265, 2486
BstMBI GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
BstMWI GCNNNNNNNGC 7 cut(s) 898, 1296, 1994, 2285, 2306, 2330, 3122
BstNI CCWGG 7 cut(s) 922, 1485, 1629, 1735, 1903, 2031, 2531
BstNSI RCATGY 3 cut(s) 1625, 2214, 2348
BstPAI GACNNNNGTC 1 cut(s) 1655
BstSCI CCNGG 9 cut(s) 920, 1483, 1627, 1733, 1802, 1901, 2029, 2380, 2529
BstSFI CTRYAG 5 cut(s) 21, 288, 742, 1174, 3117
BstSLI GKGCMC 1 cut(s) 920
BstV1I GCAGC 6 cut(s) 199, 1832, 2803, 2895, 3085, 3103
BstV2I GAAGAC 3 cut(s) 1392, 1586, 1646
BstX2I RGATCY 2 cut(s) 1148, 2461
BstYI RGATCY 2 cut(s) 1148, 2461
BsuI GTATCC 2 cut(s) 2560, 3082
BsuRI GGCC 5 cut(s) 1699, 1851, 2191, 2206, 2267
BtrI CACGTC 1 cut(s) 1646
BtsI GCAGTG 2 cut(s) 739, 2221
BtsIMutI CAGTG 3 cut(s) 319, 739, 2221
BveI ACCTGC 1 cut(s) 1511
Cac8I GCNNGC 7 cut(s) 17, 826, 940, 1518, 1697, 1853, 2946
CciI TCATGA 1 cut(s) 1420
CfoI GCGC 5 cut(s) 115, 944, 1857, 2251, 2796
Cfr10I RCCGGY 4 cut(s) 541, 1392, 1550, 2288
Cfr13I GGNCC 1 cut(s) 539
CseI GACGC 1 cut(s) 752
Csp6I GTAC 9 cut(s) 275, 457, 672, 1331, 1504, 1719, 1769, 2167, 2642
CspAI ACCGGT 2 cut(s) 541, 1392
CviQI GTAC 9 cut(s) 275, 457, 672, 1331, 1504, 1719, 1769, 2167, 2642
DinI GGCGCC 1 cut(s) 114
DpnI GATC 7 cut(s) 714, 1150, 1319, 1363, 2201, 2463, 2823
DpnII GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
DraI TTTAAA 3 cut(s) 1126, 1750, 2476
DraIII CACNNNGTG 1 cut(s) 454
DriI GACNNNNNGTC 2 cut(s) 1393, 1429
EaeI YGGCCR 1 cut(s) 2265
Eam1104I CTCTTC 8 cut(s) 372, 505, 882, 1044, 1734, 2748, 3018, 3138
Eam1105I GACNNNNNGTC 2 cut(s) 1393, 1429
EarI CTCTTC 8 cut(s) 372, 505, 882, 1044, 1734, 2748, 3018, 3138
Eco130I CCWWGG 2 cut(s) 1924, 2838
Eco147I AGGCCT 1 cut(s) 2206
Eco31I GGTCTC 1 cut(s) 809
Eco47I GGWCC 1 cut(s) 539
Eco47III AGCGCT 1 cut(s) 943
Eco57I CTGAAG 3 cut(s) 486, 1649, 2766
Eco88I CYCGRG 1 cut(s) 2955
EcoNI CCTNNNNNAGG 3 cut(s) 1632, 2534, 2710
EcoRI GAATTC 3 cut(s) 775, 1557, 2445
EcoRII CCWGG 7 cut(s) 920, 1483, 1627, 1733, 1901, 2029, 2529
EcoT14I CCWWGG 2 cut(s) 1924, 2838
EcoT22I ATGCAT 2 cut(s) 149, 1613
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
ErhI CCWWGG 2 cut(s) 1924, 2838
Esp3I CGTCTC 1 cut(s) 2265
FalI AAGNNNNNCTT 4 cut(s) 484, 516, 2745, 2777
FaqI GGGAC 4 cut(s) 552, 1187, 1487, 2985
FauNDI CATATG 2 cut(s) 1230, 2082
FbaI TGATCA 1 cut(s) 712
FblI GTMKAC 2 cut(s) 686, 2568
Fnu4HI GCNGC 8 cut(s) 213, 1067, 1700, 1821, 2792, 2909, 3099, 3117
Fsp4HI GCNGC 8 cut(s) 213, 1067, 1700, 1821, 2792, 2909, 3099, 3117
FspBI CTAG 3 cut(s) 27, 1994, 2325
GlaI GCGC 5 cut(s) 114, 943, 1856, 2250, 2795
GluI GCNGC 8 cut(s) 213, 1067, 1700, 1821, 2792, 2909, 3099, 3117
GsaI CCCAGC 2 cut(s) 3015, 3116
GsuI CTGGAG 5 cut(s) 1065, 1133, 1191, 1995, 2552
HaeII RGCGCY 4 cut(s) 116, 945, 1858, 2797
HaeIII GGCC 5 cut(s) 1699, 1851, 2191, 2206, 2267
HapII CCGG 6 cut(s) 542, 1393, 1551, 1803, 2289, 2382
HgaI GACGC 1 cut(s) 752
HhaI GCGC 5 cut(s) 115, 944, 1857, 2251, 2796
Hin1I GRCGYC 3 cut(s) 113, 763, 2258
Hin6I GCGC 5 cut(s) 113, 942, 1855, 2249, 2794
HinP1I GCGC 5 cut(s) 113, 942, 1855, 2249, 2794
HincII GTYRAC 1 cut(s) 2569
HindII GTYRAC 1 cut(s) 2569
HindIII AAGCTT 1 cut(s) 899
HpaII CCGG 6 cut(s) 542, 1393, 1551, 1803, 2289, 2382
HphI GGTGA 6 cut(s) 320, 416, 815, 1073, 1723, 2303
Hpy166II GTNNAC 8 cut(s) 58, 457, 687, 918, 1504, 2219, 2350, 2569
Hpy8I GTNNAC 8 cut(s) 58, 457, 687, 918, 1504, 2219, 2350, 2569
Hpy99I CGWCG 1 cut(s) 768
HpyCH4III ACNGT 8 cut(s) 191, 314, 404, 671, 684, 1201, 1468, 3038
HpyCH4IV ACGT 3 cut(s) 1645, 2119, 2258
HpyF10VI GCNNNNNNNGC 7 cut(s) 898, 1296, 1994, 2285, 2306, 2330, 3122
HpySE526I ACGT 3 cut(s) 1645, 2119, 2258
Hsp92I GRCGYC 3 cut(s) 113, 763, 2258
HspAI GCGC 5 cut(s) 113, 942, 1855, 2249, 2794
KasI GGCGCC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 712
Kzo9I GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
LguI GCTCTTC 1 cut(s) 882
LmnI GCTCC 6 cut(s) 1084, 1348, 1717, 2321, 2716, 2891
Lsp1109I GCAGC 6 cut(s) 199, 1832, 2803, 2895, 3085, 3103
LweI GCATC 9 cut(s) 504, 559, 881, 925, 1318, 1598, 1929, 2644, 3064
MaeI CTAG 3 cut(s) 27, 1994, 2325
MaeII ACGT 3 cut(s) 1645, 2119, 2258
MaeIII GTNAC 7 cut(s) 308, 678, 803, 1219, 1934, 2421, 2917
MalI GATC 7 cut(s) 714, 1150, 1319, 1363, 2201, 2463, 2823
MboI GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
MfeI CAATTG 2 cut(s) 655, 2337
MflI RGATCY 2 cut(s) 1148, 2461
MhlI GDGCHC 1 cut(s) 920
MlsI TGGCCA 1 cut(s) 2267
MluNI TGGCCA 1 cut(s) 2267
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 4 cut(s) 389, 1418, 1549, 2086
MmeI TCCRAC 1 cut(s) 2869
Mox20I TGGCCA 1 cut(s) 2267
Mph1103I ATGCAT 2 cut(s) 149, 1613
MroXI GAANNNNTTC 3 cut(s) 2670, 2757, 2813
MscI TGGCCA 1 cut(s) 2267
MslI CAYNNNNRTG 3 cut(s) 1452, 1458, 1515
Msp20I TGGCCA 1 cut(s) 2267
MspA1I CMGCKG 1 cut(s) 3116
MspI CCGG 6 cut(s) 542, 1393, 1551, 1803, 2289, 2382
MspR9I CCNGG 9 cut(s) 922, 1485, 1629, 1735, 1804, 1903, 2031, 2382, 2531
MunI CAATTG 2 cut(s) 655, 2337
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 7 cut(s) 922, 1485, 1629, 1735, 1903, 2031, 2531
MwoI GCNNNNNNNGC 7 cut(s) 898, 1296, 1994, 2285, 2306, 2330, 3122
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 2 cut(s) 1804, 2382
NdeI CATATG 2 cut(s) 1230, 2082
NdeII GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
NlaIV GGNNCC 5 cut(s) 114, 540, 2287, 2359, 2927
NmeAIII GCCGAG 2 cut(s) 89, 440
NmuCI GTSAC 3 cut(s) 308, 678, 803
NsiI ATGCAT 2 cut(s) 149, 1613
NspI RCATGY 3 cut(s) 1625, 2214, 2348
NspV TTCGAA 1 cut(s) 527
PagI TCATGA 1 cut(s) 1420
PceI AGGCCT 1 cut(s) 2206
PciSI GCTCTTC 1 cut(s) 882
PctI GAATGC 1 cut(s) 149
PdmI GAANNNNTTC 3 cut(s) 2670, 2757, 2813
PfeI GAWTC 6 cut(s) 182, 284, 419, 962, 2606, 2758
Pfl23II CGTACG 1 cut(s) 2641
PflFI GACNNNGTC 1 cut(s) 1199
PfoI TCCNGGA 2 cut(s) 1802, 1901
PinAI ACCGGT 2 cut(s) 541, 1392
PkrI GCNGC 8 cut(s) 214, 1068, 1701, 1822, 2793, 2910, 3100, 3118
PleI GAGTC 4 cut(s) 389, 1418, 1548, 2085
PluTI GGCGCC 1 cut(s) 116
PpsI GAGTC 4 cut(s) 389, 1418, 1548, 2085
PshAI GACNNNNGTC 1 cut(s) 1655
PshBI ATTAAT 2 cut(s) 1191, 2694
PsiI TTATAA 1 cut(s) 1706
Psp6I CCWGG 7 cut(s) 920, 1483, 1627, 1733, 1901, 2029, 2529
PspFI CCCAGC 2 cut(s) 3011, 3112
PspGI CCWGG 7 cut(s) 920, 1483, 1627, 1733, 1901, 2029, 2529
PspLI CGTACG 1 cut(s) 2641
PspN4I GGNNCC 5 cut(s) 114, 540, 2287, 2359, 2927
PspPI GGNCC 1 cut(s) 539
PstI CTGCAG 2 cut(s) 746, 3121
PsuI RGATCY 2 cut(s) 1148, 2461
PsyI GACNNNGTC 1 cut(s) 1199
PvuII CAGCTG 1 cut(s) 3116
RsaI GTAC 9 cut(s) 276, 458, 673, 1332, 1505, 1720, 1770, 2168, 2643
RsaNI GTAC 9 cut(s) 275, 457, 672, 1331, 1504, 1719, 1769, 2167, 2642
RseI CAYNNNNRTG 3 cut(s) 1452, 1458, 1515
SalI GTCGAC 1 cut(s) 2567
SapI GCTCTTC 1 cut(s) 882
SatI GCNGC 8 cut(s) 213, 1067, 1700, 1821, 2792, 2909, 3099, 3117
Sau3AI GATC 7 cut(s) 712, 1148, 1317, 1361, 2199, 2461, 2821
Sau96I GGNCC 1 cut(s) 539
SchI GAGTC 4 cut(s) 389, 1418, 1549, 2086
ScrFI CCNGG 9 cut(s) 922, 1485, 1629, 1735, 1804, 1903, 2031, 2382, 2531
SduI GDGCHC 1 cut(s) 920
SfaNI GCATC 9 cut(s) 504, 559, 881, 925, 1318, 1598, 1929, 2644, 3064
SfcI CTRYAG 5 cut(s) 21, 288, 742, 1174, 3117
SfoI GGCGCC 1 cut(s) 114
SfuI TTCGAA 1 cut(s) 527
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 3 cut(s) 1452, 1458, 1515
SmlI CTYRAG 2 cut(s) 1826, 3167
SmoI CTYRAG 2 cut(s) 1826, 3167
SseBI AGGCCT 1 cut(s) 2206
SsiI CCGC 2 cut(s) 1067, 1700
SspDI GGCGCC 1 cut(s) 112
SspI AATATT 1 cut(s) 2014
SspMI CTAG 3 cut(s) 27, 1994, 2325
StuI AGGCCT 1 cut(s) 2206
StyD4I CCNGG 9 cut(s) 920, 1483, 1627, 1733, 1802, 1901, 2029, 2380, 2529
StyI CCWWGG 2 cut(s) 1924, 2838
TaaI ACNGT 8 cut(s) 191, 314, 404, 671, 684, 1201, 1468, 3038
TaiI ACGT 3 cut(s) 1648, 2122, 2261
TaqI TCGA 9 cut(s) 180, 422, 527, 965, 1764, 2065, 2396, 2568, 2937
TaqII GACCGA 1 cut(s) 666
TatI WGTACW 3 cut(s) 274, 456, 2166
TauI GCSGC 2 cut(s) 1069, 1702
TfiI GAWTC 6 cut(s) 182, 284, 419, 962, 2606, 2758
TscAI CASTG 3 cut(s) 319, 746, 2221
TseFI GTSAC 3 cut(s) 308, 678, 803
TseI GCWGC 6 cut(s) 212, 1820, 2791, 2908, 3098, 3116
Tsp45I GTSAC 3 cut(s) 308, 678, 803
TspGWI ACGGA 2 cut(s) 1032, 3107
TspRI CASTG 3 cut(s) 319, 746, 2221
Tth111I GACNNNGTC 1 cut(s) 1199
VneI GTGCAC 1 cut(s) 916
VpaK11BI GGWCC 1 cut(s) 539
VspI ATTAAT 2 cut(s) 1191, 2694
XagI CCTNNNNNAGG 3 cut(s) 1632, 2534, 2710
XceI RCATGY 3 cut(s) 1625, 2214, 2348
XmiI GTMKAC 2 cut(s) 686, 2568
XmnI GAANNNNTTC 3 cut(s) 2670, 2757, 2813
XspI CTAG 3 cut(s) 27, 1994, 2325
ZraI GACGTC 1 cut(s) 2259
Zsp2I ATGCAT 2 cut(s) 149, 1613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.