FvH4_7g20540

SNF2 domain-containing protein CLASSY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001072
Physical Location & Seq
Forward (+)
16684637 .. 16691310
6674 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20540.t5

Sequence Viewer

Length: 3918 bp
ATGGATTATAGGCCTGTCTCAGCCAGAACAAGGTCGAAGACACTGTTGAAACTTAATGGGGAATTGAAGAAGAGCAGTGGGGTGACTCCAGCCTCAAGAAGTGAGAAAATGAGGAGGTCCAACCAGGCTTCTGTTTCTCATGTGGGTGAGTCTAGTACTAGGAAACAGAGTTTTGAGTGGGTTGCTGGTAATGTGGAATATCTGGATAATCAACAGAATTATGAAGTTTCAGTGAGTTTTGATTCTCGAGAGGGTGTCACAAAGATGATGATGGATAGTGATGATGTTGAAGTAGTAGTTTCTAATGGTAAAGAGGGAGGAAGAGGAGGAGTTGATGTTGATGATGTGAGTGATTGTGGAAGTGATGAGGATGTGTCATCAACAGAGGGAGACGACAGTAGTGATGGAAGTGTGTCTCCGACAGAAAAAAAGTCCTCTGAGGAAGAATATGATGAGGAAAGTGATGAAAGTGTGTCCTCGACAGAGGAGGCAGAATCTGATGAGGAAAGTGTTTCCTCATCAGATGAAGACAGCAGTGAGGAGGAAAAGGAAGAGGAGACCGAGTTGACAGGGAAGGATGAGAAAGAGTGCAAAGGAGAAAGTGTGGATGTTGGTTTGAAGAGAAAGAGAAATGATGAGGATGATCTGCATTGTTCTTATGGTCCGGATATTGATGACAGAAGCGAGAAAGTAAGCAAAGGGGAAACTGTTGAGGTTGGTCTGGAAGGAAAGATAATTGATAACTGTGATGATCTACATTCTTCTTCTTCTTCTTCTGGTCATGATATCAATGACAGAAGTGATCCATTGGGGCAGGCAGAGGCTGTCTTGGCTGAAGTGAGAACCGGGTGTGATAAAAGTGATGATTACAGGGATGAGAGAGTTTGGAAAGAACAAAGTGTTGGTGTTGGTTCAAAAGAAAACAGAACTGGTGATGTGCGTAATCAATGCAAAAATTCTGAAGGGGACAGTTCCCTTACCAAGAAAAAGAGCTCAAGGGCACCAATACAGAAAAGGGTACCTACTGTTGCATTTCAACCCCCAAAAGTTGAAGGTCTTCCTTTGAGGTTCACTTTTGTTAAGCAGCCAACTATTCCTGAGAAGTCAGACTATGACATAGAAGTTGAAAACCTTTTGGTCGAATATAAATTTGCTATGGCATATGACAATGCTTCTACTATTTTGAAGGCTAAAGAAAACAACTTTACAAGATCTGATAATAACAGAAACGATGTCAATGCTGAGACCTATGGTGGTAGTTCAGAGACGGCAATTAATAATGGGAGAACAAGTGATTTGCAGACGTTACTCAACGAGAATGAAGCTGCCAAAGGAAATCACGGTGAGATGCATGGTGGTACTTCGGAATCTGGTTTCACTAAAGGCAGTAAATTTGATGTGCAGAGTGTTTGCAAGAAGGGTGTCAAGGCCAAAGGAAACAAGTTTTCAAGGTCCAATAATACAGGAAAGGATGACAATGGTGAGACATATGGTGATGGTCATCTGAAAGCATATAAGAAGAAAGCAGGGGTTAAGAATCTTGCCAACAAAAAGGGCACTAGAGCACCAAAAGAGTGCAATGCGTTTTCGATTCTTTTAGATTCAATTTATGACAAGGAAGAAGTGTCAGGGAAAAAATTACCTCATTGTTGGGATAAATCCCTCAGAGATGAAGCTCCAAAAGATGATGCTCCAAAAGATGAGTCTCTTCCATTGTTTTTCACGTTTGGTCATCCGCCAAGTCCTCCACCAGAGAAGTCAGATTTTGAGATAGAAATAGATAACCTTTTTGACGAATATAACTTAGTCGGTATATCTGGCGAGATTGGTTCCACTGATTTTAATGTGGTTGAAACTAATGACTCAGTACCTCCTGGTAAGGAAGTTACTCAGCATGTCCTTTGTAGTCAAGGGAAACACAATCTTGTACTTGATGAAGAAATAGGACTTATATGTCGATTCTGCCGTCATGTGCATCGGGAGATCAAATATATTTTACCAGACTTTGCTCCAAACCCTTATGGAAAATATGGGAAGAGGTTCTATGAAGAAGGCAATCAGTCTATCCTTGATGATCTTCAGTGTAATGACCATAACGATTCTGGAGTTAGTTTCCATTCTCATACTGAAGGCACAGTGTGGGACTTGATCCCTGGTGTGGAAAGTGATATGTATCCTCATCAGCGTGATGGTTTTGAATTTATTTGGAGGCACTTAGCGGGTGGAACTCAGCTTAAAAAGTTGGCACAACCTACTGATTATGAAGGTGATGGATGCATTATATCACATGCACCTGGAACTGGGAAGACTCGTCTGACTATCATCTTTATCCAGTCATACATGAAGTTCAACCCATTAAGCAGGCCACTCATAGTTGCTCCACGCACCATGCTCCTTACTTGGGAAGAGGAGTTTAAGAAATGGGGTTTTGACATTCCCTTTCACAATCTTAACAATCCTGACTTCACTGGGAAAGAGGATGAAGCAGCTCGCAACCTTGTGAAGGATGGTAAAAAGTCAGAAATGACAAACTTGACCCGTGTAGTGAAGCTAATGTCCTGGGCAAAGACAAGAAGCATCTTGGGGATTAGTTATTCACTGTTTGAGCAGATTTGTGGAGTTCATACCAAGAAAGGAAAACATCCACTTTCTCCAAGACGAGTAGAAGAAGTTGAGCAAATGAGTAAAATCCTTCTTGAATTTCCTGGTCTTGTAGTATTTGATGAAGGGCACAATCCTCGCAACGAGCAGAGTTACTCATGGAATACTCTTTTGAAACTGAAAACAAGAAGGCGCATTCTCCTCTCTGGAACTCCTTTTCAGAATAGTTTTGAGGAGCTCCACAATACAGTCCGTTTGGTGAGGCCTGAGTGGACCACCAATCTTACCTGCCTTGGAGGTTTTGACAAAAAGCAACGGCCTTCACACCAAACGCGAGGCAGAAGAAGCAACGTAAGGAAAGGCCATTTTGCTGATGATGATAATTCAAAACGAGCCAGAGAGGTCAGGAAAATGATTTCCCCATTTGTGCATGTATACAAAGGCACCATACTGCAAGACAAGCTTCCCGGGTTAAGGAACTCAGTTGTCGTTTTACAGCCAACAGCTTTACAGCAGAGATATTTAGACAGAATTCAAAAGATTCGAAGTACATTCAAGTATGAAGTAATAGAGGCTAGAATAACTATCCACCCTTCTCTATTGCTTAATGAAGAACGTTCTACGTTTTTTGAGGAAGATGAGTATTCAGGTGACTGGGAAAGCCTAACTGAACTGAAGTTAAATCCTGGATGTGGGGTTAAGGCAAGATTTGCAGTTGATCTCATTCGCCTTTGTCAAGCTACGAATGAAAGAGTTTTGGTATTCAGTCAGTGGGTTGATCCATTGTACTTGATTAGGGACCTGCTCATATCTCAGTTTCGGTGGGTGGAAGGAGACGAGGTCATATATATGGATGGAAAATGTGATGCAAAGCAACGCCAATACTCAATGAGTAATTTCAATGATCCATCCACCAAAGTAAAAGTACTGTTAGCAACAACTAAAGGCTGTAACGAAGGAATAAGTTTAGTTGGAGCTTCAAGGGTTGTACTACTTGACGTTGCATGGAACCCATCTGTCGAGAGGCAAGCAATATCACGAGCATACAGATTGGGACAGAAAAAAATTGTGTCCGTCTACCATCTTCTCATGGCTGGGACAAGAGAAGAGGGCAAATACAACCGCCAGGTTGAGAAGCATCGATTGTCTGAATTGGTCTTTTCTTGTTCGGACGCAGAAGCTGCCAAGAAAATCCCAGCAGCCGTTGCAGAGGACAAGATTTTGGAAGAGATGGTTCAGCACCATAAACTGAAGCATATATTTGAACAGATAACATTGCTGGATGAAGCCTCATATTTGGATGAAGTTTTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1306

Amino Acids

146.84

Weight (kDa)

5.45

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 744 - 1061 3.1e-24 SNF2-related domain
ResIII PF04851 757 - 957 2.4e-09 Type III restriction enzyme, res subunit
Helicase_C PF00271 1123 - 1236 2.6e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1953
Acc36I ACCTGC 2 cut(s) 2897, 3411
Acc65I GGTACC 1 cut(s) 1018
AccB1I GGYRCC 3 cut(s) 1000, 1018, 3044
AccI GTMKAC 2 cut(s) 3036, 3678
AccII CGCG 1 cut(s) 2935
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 3 cut(s) 1736, 2219, 3724
AclI AACGTT 1 cut(s) 3217
AclWI GGATC 4 cut(s) 797, 2143, 3374, 3500
AcsI RAATTY 6 cut(s) 955, 1148, 1391, 2198, 2699, 3132
AcuI CTGAAG 6 cut(s) 855, 981, 2063, 2148, 3296, 3872
AdeI CACNNNGTG 1 cut(s) 2139
AfaI GTAC 9 cut(s) 157, 1020, 1360, 1869, 1929, 3151, 3389, 3528, 3591
AfiI CCNNNNNNNGG 7 cut(s) 30, 2158, 2300, 2401, 2503, 3075, 3293
AjnI CCWGG 8 cut(s) 123, 1873, 2152, 2293, 2558, 2704, 3286, 3726
AjuI GAANNNNNNNTTGG 2 cut(s) 885, 917
Alw21I GWGCWC 3 cut(s) 995, 1567, 2841
Alw26I GTCTC 9 cut(s) 22, 384, 420, 551, 1238, 1259, 1478, 1710, 3429
AlwI GGATC 4 cut(s) 797, 2143, 3374, 3500
AlwNI CAGNNNCTG 3 cut(s) 497, 824, 3782
Ama87I CYCGRG 2 cut(s) 246, 3068
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 6 cut(s) 11, 1428, 2363, 2864, 2918, 2962
ApeKI GCWGC 5 cut(s) 1084, 1325, 2486, 3782, 3800
ApoI RAATTY 6 cut(s) 955, 1148, 1391, 2198, 2699, 3132
ArsI GACNNNNNNTTYG 2 cut(s) 1688, 1720
AseI ATTAAT 1 cut(s) 1275
Asp700I GAANNNNTTC 2 cut(s) 1056, 2039
Asp718I GGTACC 1 cut(s) 1018
AspLEI GCGC 1 cut(s) 2796
AspS9I GGNCC 5 cut(s) 117, 662, 1452, 2874, 3400
AsuC2I CCSGG 3 cut(s) 847, 3069, 3070
AsuHPI GGTGA 9 cut(s) 94, 158, 944, 1355, 1493, 1505, 2279, 2872, 3263
AsuII TTCGAA 1 cut(s) 3145
AvaI CYCGRG 2 cut(s) 246, 3068
AvaII GGWCC 5 cut(s) 117, 662, 1452, 2874, 3400
BaeGI GKGCMC 3 cut(s) 1003, 1559, 2733
BanI GGYRCC 3 cut(s) 1000, 1018, 3044
BanII GRGCYC 2 cut(s) 995, 2841
BauI CACGAG 1 cut(s) 3639
BbsI GAAGAC 4 cut(s) 44, 534, 1049, 2312
Bbv12I GWGCWC 3 cut(s) 995, 1567, 2841
BbvI GCAGC 5 cut(s) 1096, 1312, 2498, 3769, 3812
BceAI ACGGC 4 cut(s) 1284, 1950, 2933, 3788
BcgI CGANNNNNNTGC 4 cut(s) 1220, 1254, 2720, 2754
BciT130I CCWGG 8 cut(s) 125, 1875, 2154, 2295, 2560, 2706, 3288, 3728
BciVI GTATCC 1 cut(s) 2184
BcnI CCSGG 3 cut(s) 847, 3069, 3070
BcoDI GTCTC 9 cut(s) 22, 384, 420, 551, 1238, 1259, 1478, 1710, 3429
BfaI CTAG 4 cut(s) 153, 159, 1560, 3177
BfuAI ACCTGC 2 cut(s) 2897, 3411
BfuI GTATCC 1 cut(s) 2184
BglII AGATCT 1 cut(s) 1211
BisI GCNGC 5 cut(s) 1085, 1326, 2487, 3783, 3801
BlsI GCNGC 5 cut(s) 1086, 1327, 2488, 3784, 3802
BmcAI AGTACT 2 cut(s) 157, 3528
Bme18I GGWCC 5 cut(s) 117, 662, 1452, 2874, 3400
BmeT110I CYCGRG 2 cut(s) 246, 3068
BmgT120I GGNCC 5 cut(s) 117, 662, 1452, 2874, 3400
BmiI GGNNCC 6 cut(s) 1002, 1020, 1831, 3046, 3401, 3611
BmrI ACTGGG 3 cut(s) 2310, 2478, 3265
BmsI GCATC 7 cut(s) 1338, 1678, 1984, 2264, 2586, 3457, 3748
BmuI ACTGGG 3 cut(s) 2310, 2478, 3265
BpiI GAAGAC 4 cut(s) 44, 534, 1049, 2312
BpmI CTGGAG 2 cut(s) 72, 2124
Bpu14I TTCGAA 1 cut(s) 3145
BpuEI CTTGAG 2 cut(s) 79, 979
BpuMI CCSGG 3 cut(s) 847, 3069, 3070
Bsa29I ATCGAT 1 cut(s) 3742
BsaBI GATNNNNATC 2 cut(s) 1500, 2172
BsaI GGTCTC 2 cut(s) 551, 1238
BsaJI CCNNGG 4 cut(s) 2152, 2559, 2893, 3068
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 7 cut(s) 30, 2158, 2300, 2401, 2503, 3075, 3293
Bse1I ACTGG 5 cut(s) 934, 2305, 2332, 2473, 3260
Bse3DI GCAATG 2 cut(s) 1585, 3875
Bse8I GATNNNNATC 2 cut(s) 1500, 2172
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 8 cut(s) 125, 1875, 2154, 2295, 2560, 2706, 3288, 3728
BseCI ATCGAT 1 cut(s) 3742
BseDI CCNNGG 4 cut(s) 2152, 2559, 2893, 3068
BseJI GATNNNNATC 2 cut(s) 1500, 2172
BseLI CCNNNNNNNGG 7 cut(s) 30, 2158, 2300, 2401, 2503, 3075, 3293
BseMI GCAATG 2 cut(s) 1585, 3875
BseNI ACTGG 5 cut(s) 934, 2305, 2332, 2473, 3260
BseRI GAGGAG 9 cut(s) 127, 339, 342, 500, 554, 569, 2423, 2792, 2849
BseSI GKGCMC 3 cut(s) 1003, 1559, 2733
BseXI GCAGC 5 cut(s) 1096, 1312, 2498, 3769, 3812
BseYI CCCAGC 2 cut(s) 3695, 3796
BsgI GTGCAG 1 cut(s) 1421
Bsh1236I CGCG 1 cut(s) 2935
BshFI GGCC 6 cut(s) 13, 1430, 2365, 2866, 2920, 2964
BshNI GGYRCC 3 cut(s) 1000, 1018, 3044
BshVI ATCGAT 1 cut(s) 3742
BsiHKAI GWGCWC 3 cut(s) 995, 1567, 2841
BsiHKCI CYCGRG 2 cut(s) 246, 3068
BsiSI CCGG 3 cut(s) 665, 846, 3069
BslFI GGGAC 5 cut(s) 980, 2156, 3413, 3669, 3712
BslI CCNNNNNNNGG 7 cut(s) 30, 2158, 2300, 2401, 2503, 3075, 3293
BsmAI GTCTC 9 cut(s) 22, 384, 420, 551, 1238, 1259, 1478, 1710, 3429
BsmBI CGTCTC 3 cut(s) 384, 1259, 3429
BsmFI GGGAC 5 cut(s) 980, 2156, 3413, 3669, 3712
BsmI GAATGC 1 cut(s) 2796
BsnI GGCC 6 cut(s) 13, 1430, 2365, 2866, 2920, 2964
Bso31I GGTCTC 2 cut(s) 551, 1238
BsoBI CYCGRG 2 cut(s) 246, 3068
Bsp119I TTCGAA 1 cut(s) 3145
Bsp1286I GDGCHC 6 cut(s) 995, 1003, 1559, 1567, 2733, 2841
Bsp13I TCCGGA 1 cut(s) 664
BspACI CCGC 3 cut(s) 1736, 2219, 3724
BspANI GGCC 6 cut(s) 13, 1430, 2365, 2866, 2920, 2964
BspDI ATCGAT 1 cut(s) 3742
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 1 cut(s) 2935
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 6 cut(s) 1002, 1020, 1831, 3046, 3401, 3611
BspMI ACCTGC 2 cut(s) 2897, 3411
BspPI GGATC 4 cut(s) 797, 2143, 3374, 3500
BspQI GCTCTTC 1 cut(s) 65
BspT104I TTCGAA 1 cut(s) 3145
BspT107I GGYRCC 3 cut(s) 1000, 1018, 3044
BspTNI GGTCTC 2 cut(s) 551, 1238
BsrDI GCAATG 2 cut(s) 1585, 3875
BsrI ACTGG 5 cut(s) 934, 2305, 2332, 2473, 3260
BssECI CCNNGG 4 cut(s) 2152, 2559, 2893, 3068
BssNAI GTATAC 1 cut(s) 3037
BssSI CACGAG 1 cut(s) 3639
BssT1I CCWWGG 1 cut(s) 2893
Bst1107I GTATAC 1 cut(s) 3037
Bst2BI CACGAG 1 cut(s) 3639
Bst2UI CCWGG 8 cut(s) 125, 1875, 2154, 2295, 2560, 2706, 3288, 3728
Bst6I CTCTTC 9 cut(s) 65, 316, 546, 614, 1713, 2030, 2400, 3702, 3822
BstAPI GCANNNNNTGC 3 cut(s) 3311, 3782, 3806
BstBI TTCGAA 1 cut(s) 3145
BstC8I GCNNGC 4 cut(s) 816, 2363, 2491, 3630
BstENI CCTNNNNNAGG 1 cut(s) 2501
BstFNI CGCG 1 cut(s) 2935
BstHHI GCGC 1 cut(s) 2796
BstMAI GTCTC 9 cut(s) 22, 384, 420, 551, 1238, 1259, 1478, 1710, 3429
BstMWI GCNNNNNNNGC 6 cut(s) 830, 2946, 3061, 3311, 3782, 3806
BstNI CCWGG 8 cut(s) 125, 1875, 2154, 2295, 2560, 2706, 3288, 3728
BstNSI RCATGY 3 cut(s) 1898, 2291, 3035
BstSLI GKGCMC 3 cut(s) 1003, 1559, 2733
BstUI CGCG 1 cut(s) 2935
BstV1I GCAGC 5 cut(s) 1096, 1312, 2498, 3769, 3812
BstV2I GAAGAC 4 cut(s) 44, 534, 1049, 2312
BstX2I RGATCY 1 cut(s) 1211
BstYI RGATCY 1 cut(s) 1211
BstZ17I GTATAC 1 cut(s) 3037
Bsu15I ATCGAT 1 cut(s) 3742
BsuI GTATCC 1 cut(s) 2184
BsuRI GGCC 6 cut(s) 13, 1430, 2365, 2866, 2920, 2964
BsuTUI ATCGAT 1 cut(s) 3742
BtsI GCAGTG 2 cut(s) 82, 541
BveI ACCTGC 2 cut(s) 2897, 3411
Cac8I GCNNGC 4 cut(s) 816, 2363, 2491, 3630
CaiI CAGNNNCTG 3 cut(s) 497, 824, 3782
CciI TCATGA 1 cut(s) 781
CfoI GCGC 1 cut(s) 2796
Cfr13I GGNCC 5 cut(s) 117, 662, 1452, 2874, 3400
Cfr9I CCCGGG 1 cut(s) 3068
ClaI ATCGAT 1 cut(s) 3742
CseI GACGC 1 cut(s) 3782
Csp6I GTAC 9 cut(s) 156, 1019, 1359, 1868, 1928, 3150, 3388, 3527, 3590
CviQI GTAC 9 cut(s) 156, 1019, 1359, 1868, 1928, 3150, 3388, 3527, 3590
DraIII CACNNNGTG 1 cut(s) 2139
DrdI GACNNNNNNGTC 1 cut(s) 1953
DseDI GACNNNNNNGTC 1 cut(s) 1953
Eam1104I CTCTTC 9 cut(s) 65, 316, 546, 614, 1713, 2030, 2400, 3702, 3822
EarI CTCTTC 9 cut(s) 65, 316, 546, 614, 1713, 2030, 2400, 3702, 3822
EciI GGCGGA 1 cut(s) 1725
Ecl136II GAGCTC 2 cut(s) 993, 2839
Eco130I CCWWGG 1 cut(s) 2893
Eco147I AGGCCT 2 cut(s) 13, 2866
Eco24I GRGCYC 2 cut(s) 995, 2841
Eco31I GGTCTC 2 cut(s) 551, 1238
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 5 cut(s) 117, 662, 1452, 2874, 3400
Eco53kI GAGCTC 2 cut(s) 993, 2839
Eco57I CTGAAG 6 cut(s) 855, 981, 2063, 2148, 3296, 3872
Eco88I CYCGRG 2 cut(s) 246, 3068
EcoICRI GAGCTC 2 cut(s) 993, 2839
EcoNI CCTNNNNNAGG 1 cut(s) 2501
EcoO109I RGGNCCY 1 cut(s) 3400
EcoRI GAATTC 1 cut(s) 3132
EcoRII CCWGG 8 cut(s) 123, 1873, 2152, 2293, 2558, 2704, 3286, 3726
EcoRV GATATC 1 cut(s) 787
EcoT14I CCWWGG 1 cut(s) 2893
EcoT22I ATGCAT 2 cut(s) 1353, 2279
EcoT38I GRGCYC 2 cut(s) 995, 2841
ErhI CCWWGG 1 cut(s) 2893
Esp3I CGTCTC 3 cut(s) 384, 1259, 3429
FalI AAGNNNNNCTT 2 cut(s) 3048, 3080
FaqI GGGAC 5 cut(s) 980, 2156, 3413, 3669, 3712
FauI CCCGC 1 cut(s) 2212
FauNDI CATATG 2 cut(s) 1162, 1489
FblI GTMKAC 2 cut(s) 3036, 3678
Fnu4HI GCNGC 5 cut(s) 1085, 1326, 2487, 3783, 3801
FriOI GRGCYC 2 cut(s) 995, 2841
Fsp4HI GCNGC 5 cut(s) 1085, 1326, 2487, 3783, 3801
FspBI CTAG 4 cut(s) 153, 159, 1560, 3177
GlaI GCGC 1 cut(s) 2795
GluI GCNGC 5 cut(s) 1085, 1326, 2487, 3783, 3801
GsaI CCCAGC 2 cut(s) 3699, 3800
GsuI CTGGAG 2 cut(s) 72, 2124
HaeIII GGCC 6 cut(s) 13, 1430, 2365, 2866, 2920, 2964
HapII CCGG 3 cut(s) 665, 846, 3069
HgaI GACGC 1 cut(s) 3782
HhaI GCGC 1 cut(s) 2796
Hin6I GCGC 1 cut(s) 2794
HinP1I GCGC 1 cut(s) 2794
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HindIII AAGCTT 1 cut(s) 3062
HpaII CCGG 3 cut(s) 665, 846, 3069
HphI GGTGA 9 cut(s) 94, 158, 944, 1355, 1493, 1505, 2279, 2872, 3263
Hpy166II GTNNAC 5 cut(s) 567, 1071, 2874, 3037, 3679
Hpy8I GTNNAC 5 cut(s) 567, 1071, 2874, 3037, 3679
HpyCH4IV ACGT 6 cut(s) 1304, 1724, 2952, 3217, 3224, 3600
HpyF10VI GCNNNNNNNGC 6 cut(s) 830, 2946, 3061, 3311, 3782, 3806
HpySE526I ACGT 6 cut(s) 1304, 1724, 2952, 3217, 3224, 3600
HspAI GCGC 1 cut(s) 2794
Kpn2I TCCGGA 1 cut(s) 664
KpnI GGTACC 1 cut(s) 1022
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 8 cut(s) 1681, 1696, 2014, 2383, 2397, 2836, 2844, 3575
Lsp1109I GCAGC 5 cut(s) 1096, 1312, 2498, 3769, 3812
LweI GCATC 7 cut(s) 1338, 1678, 1984, 2264, 2586, 3457, 3748
MaeI CTAG 4 cut(s) 153, 159, 1560, 3177
MaeII ACGT 6 cut(s) 1304, 1724, 2952, 3217, 3224, 3600
MaeIII GTNAC 7 cut(s) 82, 256, 1305, 1885, 2753, 3251, 3551
MflI RGATCY 1 cut(s) 1211
MhlI GDGCHC 6 cut(s) 995, 1003, 1559, 1567, 2733, 2841
MlyI GAGTC 5 cut(s) 79, 158, 1712, 1856, 2302
MmeI TCCRAC 3 cut(s) 144, 443, 3553
Mph1103I ATGCAT 2 cut(s) 1353, 2279
MroI TCCGGA 1 cut(s) 664
MroXI GAANNNNTTC 2 cut(s) 1056, 2039
MslI CAYNNNNRTG 4 cut(s) 144, 263, 2184, 3449
MspI CCGG 3 cut(s) 665, 846, 3069
Mva1269I GAATGC 1 cut(s) 2796
MvaI CCWGG 8 cut(s) 125, 1875, 2154, 2295, 2560, 2706, 3288, 3728
MvnI CGCG 1 cut(s) 2935
MwoI GCNNNNNNNGC 6 cut(s) 830, 2946, 3061, 3311, 3782, 3806
NciI CCSGG 3 cut(s) 847, 3069, 3070
NdeI CATATG 2 cut(s) 1162, 1489
NlaIV GGNNCC 6 cut(s) 1002, 1020, 1831, 3046, 3401, 3611
NmuCI GTSAC 3 cut(s) 82, 256, 3251
NsiI ATGCAT 2 cut(s) 1353, 2279
NspI RCATGY 3 cut(s) 1898, 2291, 3035
NspV TTCGAA 1 cut(s) 3145
PaeR7I CTCGAG 1 cut(s) 246
PagI TCATGA 1 cut(s) 781
PceI AGGCCT 2 cut(s) 13, 2866
PciSI GCTCTTC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 1963
PctI GAATGC 1 cut(s) 2796
PdmI GAANNNNTTC 2 cut(s) 1056, 2039
PfeI GAWTC 9 cut(s) 242, 494, 1367, 1537, 1591, 1601, 1959, 2099, 3142
PflFI GACNNNGTC 1 cut(s) 3440
PfoI TCCNGGA 1 cut(s) 3286
PkrI GCNGC 5 cut(s) 1086, 1327, 2488, 3784, 3802
PleI GAGTC 5 cut(s) 79, 157, 1711, 1856, 2302
PpsI GAGTC 5 cut(s) 79, 157, 1711, 1856, 2302
PpuMI RGGWCCY 1 cut(s) 3400
PshBI ATTAAT 1 cut(s) 1275
Psp124BI GAGCTC 2 cut(s) 995, 2841
Psp1406I AACGTT 1 cut(s) 3217
Psp5II RGGWCCY 1 cut(s) 3400
Psp6I CCWGG 8 cut(s) 123, 1873, 2152, 2293, 2558, 2704, 3286, 3726
PspFI CCCAGC 2 cut(s) 3695, 3796
PspGI CCWGG 8 cut(s) 123, 1873, 2152, 2293, 2558, 2704, 3286, 3726
PspN4I GGNNCC 6 cut(s) 1002, 1020, 1831, 3046, 3401, 3611
PspPI GGNCC 5 cut(s) 117, 662, 1452, 2874, 3400
PspPPI RGGWCCY 1 cut(s) 3400
PstNI CAGNNNCTG 3 cut(s) 497, 824, 3782
PsuI RGATCY 1 cut(s) 1211
PsyI GACNNNGTC 1 cut(s) 3440
RsaI GTAC 9 cut(s) 157, 1020, 1360, 1869, 1929, 3151, 3389, 3528, 3591
RsaNI GTAC 9 cut(s) 156, 1019, 1359, 1868, 1928, 3150, 3388, 3527, 3590
RseI CAYNNNNRTG 4 cut(s) 144, 263, 2184, 3449
SacI GAGCTC 2 cut(s) 995, 2841
SapI GCTCTTC 1 cut(s) 65
SatI GCNGC 5 cut(s) 1085, 1326, 2487, 3783, 3801
Sau96I GGNCC 5 cut(s) 117, 662, 1452, 2874, 3400
ScaI AGTACT 2 cut(s) 157, 3528
SchI GAGTC 5 cut(s) 79, 158, 1712, 1856, 2302
SduI GDGCHC 6 cut(s) 995, 1003, 1559, 1567, 2733, 2841
SfaNI GCATC 7 cut(s) 1338, 1678, 1984, 2264, 2586, 3457, 3748
Sfr274I CTCGAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 3145
SinI GGWCC 5 cut(s) 117, 662, 1452, 2874, 3400
SlaI CTCGAG 1 cut(s) 246
SmaI CCCGGG 1 cut(s) 3070
SmiMI CAYNNNNRTG 4 cut(s) 144, 263, 2184, 3449
SmlI CTYRAG 3 cut(s) 94, 246, 994
SmoI CTYRAG 3 cut(s) 94, 246, 994
SseBI AGGCCT 2 cut(s) 13, 2866
SsiI CCGC 3 cut(s) 1736, 2219, 3724
SspMI CTAG 4 cut(s) 153, 159, 1560, 3177
SstI GAGCTC 2 cut(s) 995, 2841
StuI AGGCCT 2 cut(s) 13, 2866
StyI CCWWGG 1 cut(s) 2893
TaiI ACGT 6 cut(s) 1307, 1727, 2955, 3220, 3227, 3603
TaqI TCGA 9 cut(s) 35, 247, 479, 1140, 1589, 1957, 3145, 3621, 3742
TaqII GACCGA 1 cut(s) 575
TatI WGTACW 6 cut(s) 155, 1927, 3149, 3387, 3526, 3589
TfiI GAWTC 9 cut(s) 242, 494, 1367, 1537, 1591, 1601, 1959, 2099, 3142
TseFI GTSAC 3 cut(s) 82, 256, 3251
TseI GCWGC 5 cut(s) 1084, 1325, 2486, 3782, 3800
Tsp45I GTSAC 3 cut(s) 82, 256, 3251
TspGWI ACGGA 2 cut(s) 2843, 3664
TspMI CCCGGG 1 cut(s) 3068
Tth111I GACNNNGTC 1 cut(s) 3440
VpaK11BI GGWCC 5 cut(s) 117, 662, 1452, 2874, 3400
VspI ATTAAT 1 cut(s) 1275
XagI CCTNNNNNAGG 1 cut(s) 2501
XapI RAATTY 6 cut(s) 955, 1148, 1391, 2198, 2699, 3132
XceI RCATGY 3 cut(s) 1898, 2291, 3035
XcmI CCANNNNNNNNNTGG 1 cut(s) 2099
XhoI CTCGAG 1 cut(s) 246
XmaI CCCGGG 1 cut(s) 3068
XmiI GTMKAC 2 cut(s) 3036, 3678
XmnI GAANNNNTTC 2 cut(s) 1056, 2039
XspI CTAG 4 cut(s) 153, 159, 1560, 3177
ZrmI AGTACT 2 cut(s) 157, 3528
Zsp2I ATGCAT 2 cut(s) 1353, 2279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.