RchiOBHm_Chr7g0206511

SNF2 domain-containing protein CLASSY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001072
Physical Location & Seq
Reverse (-)
24156863 .. 24163825
6963 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18481

Sequence Viewer

Length: 4497 bp
ATGTTTTTGGCAGGACATTGTGGAGTCCTTGTAGAGAAGTCTATGGATTATAGGCCTATATCAAGCAGAACAAGGTCGAAGAGAGAAACAGCTCTCATGGAACGTTATCGGGAAATGAAGATCAACAGTGGGGTGAGTCCAGCAAGGAGTAAGAAAATGAGGAGGTCCAACCAGGCTTCTGTTTCTCATATGAGTGAGTCCAGTTCTAGGAAACAGAGTTTTGAGTGGGTTGCTGATCATGTGGAACAGACTGATGACGATTCGGAGGGTTTTTATTCTCAAGAGAGTAGTGGGAAACAGGGAAAGGGGGAAGGAGTTGCGGTTGATGTGAAGAGAAAGAGGAGTTTAGGATTAGATGATAGCAACAAAGATGGTTGCAACTGGAGAGCTGTTTCAGATAATGGTGTGGAAGTTGTTGATTTGGTGGATAGTGATGATGAGGAGGAGGTAGTAGTTTCTAAAGGTAAAGAGGGAAGAGGAGGAGGAGGAGGAGTTGATGTTGATGATGTGAGTGATGGTGGAAGTAATGAGGATGTTGATGATGTCAGTGGTAACGAGAGTGATGAGGCCATTTCATCCGAAAGTGAGTTCTTGCCAGGGAAAGAGTCCACAGTGGAGGAAAATGATGAGGAATGTATTTCTTTTTCAGATGAAGATAGCTGTGAGGAGGGAAATGAAGAGGAGAGAGAATTGACAGAGAAGAATGAGAAAGAAAGCAAGGAGGAAAGTGTTGAGGTTGGTTTGGAGAGAAATAGAAATGCTGATGGGGATGACCTGCATTCTTCTTATGGTCATGATTTTGACGACAGCAGCAAGAAAGTAAGCAAAGGAGAATCTGTTGAGGTTGGTCTGGAAGGAAAGATAAGTGATGATCTGCATTCTTGTTCTGCTCATGATACTGATGTCAGAATTGAGCAAGTCTGGAAGGAAGAAAGTCTAAGGATTCTTCCGAAGGGAAAGATTAATGCTGATGGGGATGATCTACATTCTTCTTTTGGTCATGATATCGATGACAGAAGTGCGAAATTGGGGGAGGCAGAGAGTGTGTTGGCCGAAGAGAGAACTGGGTGTGATAGAAGTGATGACAGCAATGATGGGAAAGTGTGGCAGGAAGAAATTGTTGGGGTTGGTTCGAAGGAAAACAGAATTGGTGAGGGGTATAATCTACATTCTTCTTCTGTTCATGATATTAATGACAGAATTGAGAAATTGGGGGAGGCAGAGAGTGTGTTGGCCGAAGAGAGAATTGGGTGTGATAGAAGTGATGATAACAATGATGGGAAAGTGTGGAATGAAGAAAGTGTTGGGCTTGATTCGAAGGAAAACAGAAGTGGTGATGAGTGTAATCTACATTCTTCTTCTGTTCATGATATGAATGACAGAAGTGAGAAACTGGGAAAGGCGGGAGGTGTATATGCTGAAAGGAAAGATGGGTGGGATAAAAGAAGAGAATACGGCAGTGAGGGAAAAACTGGTTTTGGTTATAGCAGAAATGAAAACAATGGTAAATTGAAAGCAGAGTCCGAAACTGGTTCTGCTAGTAGATTAAAGGATGGCACCAAATATAAAGGAAAAACTGGGGCTGTTGGACGTAAAACTTGGTCCGGTAAATGGAATGAGGTGCATAATGAATGCAAAAAGTCCGCAGGGGATAGCTTCCTTTCTAAACAAAAGGGCACGAGGGCACCAAAACGGAAAAGGTTACCTACAGTTGCATTTGAAGCCCCAAAAGTTGAATCTCTTCCTTTGAGATTCAGTTTTTCTAAGCAGCCAACTATTCCTGAGAAGCCAGATTATGAGAGAGAAGTAGATGAACTTTTTGATGAATATAAATTTGCTATGGCATATGATGAGAATGCTTCGACTGAATCTAAGGCCAAAGGAAATGATGATGCAAGGTCAAAGAATAGGATACATGATTTCGATGATGAGACGCATGGTGGTACTTCAGAATCTGGTTTTGATAAAGGCAGTAAACTTGATGTGCAGACGGCTTGCAAGACGGGTGTAGAGGCTGAAGGAAACAACTTTTCAGAATCCATTAATTCACGAAAGGAAGACAATGATGAGGCATATGGTGGTAGTCGTCCGGAACTGTATAAGGAGCAAGAAAGGGTGAGGGAGCTTACCAACGAAAAGGGCATTACAGCACCAAAAGATTGCAATGCCTTTATGATTCTTGTAGATTCGATTTATGACAAGGGAGAAGTGTCAGGGGAACAGTTACCTCCTTCTAACGATGAATCCCCCCAGGATGAAGCTCCAAGAGATGAGCCTCTTCCAAAGTTTTTCAGGCTTGGTCATCCTCCAAGTCCTGCACCAGAGAAGTCAGCTTCTGAGATAGAACTAGAAGAACTTTTTGAACAATATGACTTTGTCCTCATGTCTGAAGGGATTGGTTCCACTGATTCTATTGTGGTTGAAACTAATGACTCAATCCCTCCCGGTAAGGAAGTTACTCAGCATATGCTTTGTAGTCAAGGAAAACATGATCTTATACTTGATGAAGAAATTGGATACATATGTCGATTCTGCCCTCATGTGCATCAGGAGATCAAGTATATTGTACCAGACTTTGCTCCAAACCCCTATGGAAAATCTGGGAAGAGATTTTATGAAGAAGACAACCGGTCTATCCTTGATGATCTTCAGTGTAATGACCCTGACGATTCTGGCTTTAGTTCCCATGCTCATACTGAAGGCACAGTGTGGGACTTGATCCCTGGTGTGGAAAGTGATATGTACCCTCATCAGCGTGAAGGTTTTGAATTTATCTGGAGGCACTTAGCTGGTGGAATCCAGCTTGGAAAGTTGGAAAAACCTACTGCTTATGGTGGTGATGGATGCATTATATCGCATGCACCTGGAACTGGAAAGACTCGTTTGACTATAATCTTTATCCAGTCGTACATGAATTTTAACCCATCAAGCAGGCCCCTCATCGTTGCTCCACGTACCATGCTCCTTACTTGGGAAGAGGAGTTTAAGAAATGGGGTTTTGACATTCCCTTTCACAATCTTAACAATCCTGATTTCACTGGGAAAGAGGATGAAGCAGCTCGCAATCTTGTGAAGGATGGTAAAAGTTCAGAAATGATAAACTTGACCCGTATCGTGAAGCTGAAGTCCTGGGCAAAGAAAAGAAGCATCTTGGGGATTAGTTATTCACTGTTTGAACAGGTATGTGGAGCCCCTACCAAGAAAGGAAAACATCCACTTTCTTCAAGACGAAAGTTCGAAGTTGAGCAGATGAGTGAAATCCTTCTTAAATTTCCTGGTCTTGTAGTATTTGACGAAGGGCACAATCCTCGCAATGACCAGAGTCACTCATGGAATGCTCTTTTAAAACTGGAAACAAGAAGGCGCATTATCCTCTCTGGTACTCCTTTTCAGAATAATTTTGAGGAGCTCTACAATACAGTCCGCTTGGTGAGGCCAGAATGGACCACCAATCTTACCTGCCTTGGAGCTTTTGACAAAAAGCAATGGACTTCGGACCAAATTCGAGGCAGAGAAGGCAGAAAAAGCAATGCTGGGAGAGGCCAATTTGCGGATGATAATACAAAGCGAGCCAAGGAGGTCAGAGGTATGATTTCCCCATTTGTGAATGTTTACAAAGGCACCATACTGCAAGATAAGCTTCCAGGGTTAAGGAACTCAGTTGTTGTTCTACAGCCAACAGCTTTACAGCAGAGATATTCTGACAGAACTCGGCTGATAAAGAATCCATTCAAATATGAAAGAATAGAGGCTAAAGTAACTATCCATCCTTCTCTCTTGCTTACTGAAGAGCGCTCTATGTTTCATGAGGGTGACGAGTATTCAGGTGACTGGGAAAGGCTAACTGAAATTAAGTTAAATCCTGAATGTGGAGTCAAGGCAAGATTTGCAGTTGATCTTATTCGGCTTTGTCAAGCTATGAAGGAAAGAGTTTTGGTATTCAGTCAGTGGCTTGATCCATTGTTCTTGATAAGGGACATGCTCAAATCTCAGTTTCAGTGGGTGGAAGGAGAGGAGGTTGTGTATATGGATGGAAAATGTGATGCAAAGCAACGCCAATCCTCAATGAGTTATTTCAATGATCCATCCTCCAAAGCAAAGGCAATGTTAGCTACAACAAAAGGTTGTAATGAAGGAATAAGTTTAGTTGGAGCTTCAAGGGTTGTACTGCTTGATGTTGCATGGAACCCATCTGTCGAGAGGCAAGCTATATCCCGAGCATACCGATTGGGACAGAAAAAGATTGTCTTCATATACCATCTCCTCATGGCTGGGGCAAGAGAAGAGGACAAATACAGCCGGCAAGTTGAGAAGCATCGGTTGTCTGAATTGGTATTTTCTTGTTCGAATACAGAAGCAGATGCCAAGAAACTCCCATCTGCGGTCGCAGAGGACAAGGTTTTGGAAGAGATGGTGCAGCATGAGAAACTCAAGCATATATTCCACAAGATAACATTACTGGATGAAGCCTCATATTTGGATGAAGATTTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

1498

Amino Acids

168.37

Weight (kDa)

5.12

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 914 - 1130 5.9e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 917 - 1242 3.9e-24 SNF2-related domain
Helicase_C PF00271 1299 - 1411 1.3e-15 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 783, 3467
AccB1I GGYRCC 3 cut(s) 1556, 1684, 3620
AccIII TCCGGA 1 cut(s) 2089
AciI CCGC 6 cut(s) 320, 1403, 1644, 3424, 3551, 4382
AclI AACGTT 1 cut(s) 103
AclWI GGATC 3 cut(s) 2713, 3950, 4076
AcoI YGGCCR 2 cut(s) 1050, 1233
AcsI RAATTY 5 cut(s) 1832, 2768, 2914, 3269, 3501
AcuI CTGAAG 7 cut(s) 1932, 2037, 2409, 2633, 2718, 3143, 3807
AdeI CACNNNGTG 1 cut(s) 2709
AfaI GTAC 7 cut(s) 1945, 2568, 2744, 2909, 2956, 3382, 4167
AfeI AGCGCT 1 cut(s) 3794
AfiI CCNNNNNNNGG 8 cut(s) 207, 1611, 2728, 2870, 2971, 3550, 3869, 4381
AgeI ACCGGT 1 cut(s) 2628
AhdI GACNNNNNGTC 1 cut(s) 2629
AjnI CCWGG 8 cut(s) 171, 595, 2250, 2722, 2863, 3128, 3274, 3643
AjuI GAANNNNNNNTTGG 8 cut(s) 1104, 1131, 1136, 1163, 1230, 1262, 1287, 1319
Alw21I GWGCWC 1 cut(s) 3411
Alw26I GTCTC 1 cut(s) 1925
AlwI GGATC 3 cut(s) 2713, 3950, 4076
AlwNI CAGNNNCTG 2 cut(s) 1955, 2336
Ama87I CYCGRG 1 cut(s) 4215
Aor13HI TCCGGA 1 cut(s) 2089
Aor51HI AGCGCT 1 cut(s) 3794
AoxI GGCC 8 cut(s) 53, 567, 1050, 1233, 1875, 2933, 3434, 3541
ApeKI GCWGC 4 cut(s) 810, 1768, 3056, 4417
ApoI RAATTY 5 cut(s) 1832, 2768, 2914, 3269, 3501
AseI ATTAAT 3 cut(s) 963, 1191, 2043
AsiGI ACCGGT 1 cut(s) 2628
Asp700I GAANNNNTTC 3 cut(s) 1740, 3261, 3728
AspLEI GCGC 2 cut(s) 3366, 3795
AspS9I GGNCC 5 cut(s) 165, 1602, 2934, 3444, 3496
AsuC2I CCSGG 1 cut(s) 2445
AsuHPI GGTGA 8 cut(s) 145, 1163, 1346, 2128, 2849, 3442, 3824, 3839
AsuII TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
AvaI CYCGRG 1 cut(s) 4215
AvaII GGWCC 4 cut(s) 165, 1602, 3444, 3496
BaeGI GKGCMC 3 cut(s) 1679, 1687, 3303
BaeI ACNNNNGTAYC 4 cut(s) 888, 921, 2509, 2542
BanI GGYRCC 3 cut(s) 1556, 1684, 3620
BanII GRGCYC 2 cut(s) 3193, 3411
BauI CACGAG 1 cut(s) 1678
BbsI GAAGAC 3 cut(s) 2064, 2628, 4240
Bbv12I GWGCWC 1 cut(s) 3411
BbvI GCAGC 4 cut(s) 822, 1780, 3068, 4429
BceAI ACGGC 2 cut(s) 1471, 2007
BcgI CGANNNNNNTGC 2 cut(s) 3290, 3324
BciT130I CCWGG 8 cut(s) 173, 597, 2252, 2724, 2865, 3130, 3276, 3645
BciVI GTATCC 2 cut(s) 1905, 2510
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 2445
BcoDI GTCTC 1 cut(s) 1925
BfaI CTAG 3 cut(s) 207, 1539, 2348
BfmI CTRYAG 2 cut(s) 1707, 3671
BfoI RGCGCY 1 cut(s) 3796
BfuAI ACCTGC 2 cut(s) 783, 3467
BfuI GTATCC 2 cut(s) 1905, 2510
BisI GCNGC 4 cut(s) 811, 1769, 3057, 4418
BlsI GCNGC 4 cut(s) 812, 1770, 3058, 4419
Bme1390I CCNGG 9 cut(s) 173, 597, 2252, 2445, 2724, 2865, 3130, 3276, 3645
Bme18I GGWCC 4 cut(s) 165, 1602, 3444, 3496
BmeRI GACNNNNNGTC 1 cut(s) 2629
BmeT110I CYCGRG 1 cut(s) 4215
BmgT120I GGNCC 5 cut(s) 165, 1602, 2934, 3444, 3496
BmiI GGNNCC 7 cut(s) 1558, 1686, 2401, 2936, 3190, 3622, 4187
BmrFI CCNGG 9 cut(s) 173, 597, 2252, 2445, 2724, 2865, 3130, 3276, 3645
BmrI ACTGGG 5 cut(s) 1074, 1403, 1587, 3048, 3841
BmsI GCATC 7 cut(s) 1882, 2554, 2834, 3156, 4033, 4324, 4351
BmuI ACTGGG 5 cut(s) 1074, 1403, 1587, 3048, 3841
BoxI GACNNNNGTC 1 cut(s) 3321
BpiI GAAGAC 3 cut(s) 2064, 2628, 4240
BpmI CTGGAG 2 cut(s) 403, 2797
Bpu14I TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
BpuEI CTTGAG 2 cut(s) 264, 4415
BpuMI CCSGG 1 cut(s) 2445
Bsa29I ATCGAT 1 cut(s) 1008
BsaAI YACGTR 1 cut(s) 2954
BsaJI CCNNGG 7 cut(s) 596, 2250, 2722, 3129, 3463, 3573, 3644
BsaWI WCCGGW 3 cut(s) 1604, 2089, 2628
Bsc4I CCNNNNNNNGG 8 cut(s) 207, 1611, 2728, 2870, 2971, 3550, 3869, 4381
Bse118I RCCGGY 2 cut(s) 2628, 4299
Bse3DI GCAATG 6 cut(s) 1096, 2170, 3319, 3491, 3535, 4110
BseAI TCCGGA 1 cut(s) 2089
BseBI CCWGG 8 cut(s) 173, 597, 2252, 2724, 2865, 3130, 3276, 3645
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 7 cut(s) 596, 2250, 2722, 3129, 3463, 3573, 3644
BseLI CCNNNNNNNGG 8 cut(s) 207, 1611, 2728, 2870, 2971, 3550, 3869, 4381
BseMI GCAATG 6 cut(s) 1096, 2170, 3319, 3491, 3535, 4110
BseMII CTCAG 5 cut(s) 1773, 2328, 2474, 3672, 4004
BseSI GKGCMC 3 cut(s) 1679, 1687, 3303
BseXI GCAGC 4 cut(s) 822, 1780, 3068, 4429
BseYI CCCAGC 2 cut(s) 3533, 4271
BsgI GTGCAG 3 cut(s) 2006, 2301, 4436
Bsh1285I CGRYCG 1 cut(s) 4386
BshFI GGCC 8 cut(s) 55, 569, 1052, 1235, 1877, 2935, 3436, 3543
BshNI GGYRCC 3 cut(s) 1556, 1684, 3620
BshTI ACCGGT 1 cut(s) 2628
BshVI ATCGAT 1 cut(s) 1008
BsiEI CGRYCG 1 cut(s) 4386
BsiHKAI GWGCWC 1 cut(s) 3411
BsiHKCI CYCGRG 1 cut(s) 4215
BsiSI CCGG 5 cut(s) 1605, 2090, 2445, 2629, 4300
BslFI GGGAC 3 cut(s) 2726, 3989, 4245
BslI CCNNNNNNNGG 8 cut(s) 207, 1611, 2728, 2870, 2971, 3550, 3869, 4381
BsmAI GTCTC 1 cut(s) 1925
BsmBI CGTCTC 1 cut(s) 1925
BsmFI GGGAC 3 cut(s) 2726, 3989, 4245
BsmI GAATGC 5 cut(s) 778, 877, 1637, 1861, 3340
BsnI GGCC 8 cut(s) 55, 569, 1052, 1235, 1877, 2935, 3436, 3543
BsoBI CYCGRG 1 cut(s) 4215
Bsp119I TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
Bsp1286I GDGCHC 5 cut(s) 1679, 1687, 3193, 3303, 3411
Bsp13I TCCGGA 1 cut(s) 2089
BspACI CCGC 6 cut(s) 320, 1403, 1644, 3424, 3551, 4382
BspANI GGCC 8 cut(s) 55, 569, 1052, 1235, 1877, 2935, 3436, 3543
BspCNI CTCAG 5 cut(s) 1774, 2329, 2473, 3671, 4003
BspDI ATCGAT 1 cut(s) 1008
BspEI TCCGGA 1 cut(s) 2089
BspHI TCATGA 6 cut(s) 793, 892, 1000, 1183, 1366, 3805
BspLI GGNNCC 7 cut(s) 1558, 1686, 2401, 2936, 3190, 3622, 4187
BspMI ACCTGC 2 cut(s) 783, 3467
BspPI GGATC 3 cut(s) 2713, 3950, 4076
BspQI GCTCTTC 1 cut(s) 3783
BspT104I TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
BspT107I GGYRCC 3 cut(s) 1556, 1684, 3620
BsrDI GCAATG 6 cut(s) 1096, 2170, 3319, 3491, 3535, 4110
BsrFI RCCGGY 2 cut(s) 2628, 4299
BssAI RCCGGY 2 cut(s) 2628, 4299
BssECI CCNNGG 7 cut(s) 596, 2250, 2722, 3129, 3463, 3573, 3644
BssSI CACGAG 1 cut(s) 1678
BssT1I CCWWGG 2 cut(s) 3463, 3573
Bst2BI CACGAG 1 cut(s) 1678
Bst2UI CCWGG 8 cut(s) 173, 597, 2252, 2724, 2865, 3130, 3276, 3645
Bst4CI ACNGT 8 cut(s) 128, 613, 1711, 2097, 2223, 2707, 3171, 3421
BstAPI GCANNNNNTGC 1 cut(s) 3887
BstBAI YACGTR 1 cut(s) 2954
BstBI TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
BstC8I GCNNGC 7 cut(s) 1996, 2859, 2933, 3061, 3570, 4206, 4301
BstDEI CTNAG 9 cut(s) 938, 1764, 1782, 1872, 2337, 2460, 2785, 3658, 3990
BstEII GGTNACC 1 cut(s) 1701
BstH2I RGCGCY 1 cut(s) 3796
BstHHI GCGC 2 cut(s) 3366, 3795
BstMAI GTCTC 1 cut(s) 1925
BstMCI CGRYCG 1 cut(s) 4386
BstMWI GCNNNNNNNGC 6 cut(s) 1721, 3516, 3525, 3637, 3887, 4109
BstNI CCWGG 8 cut(s) 173, 597, 2252, 2724, 2865, 3130, 3276, 3645
BstNSI RCATGY 2 cut(s) 2861, 3982
BstPAI GACNNNNGTC 1 cut(s) 3321
BstPI GGTNACC 1 cut(s) 1701
BstSCI CCNGG 9 cut(s) 171, 595, 2250, 2443, 2722, 2863, 3128, 3274, 3643
BstSFI CTRYAG 2 cut(s) 1707, 3671
BstSLI GKGCMC 3 cut(s) 1679, 1687, 3303
BstV1I GCAGC 4 cut(s) 822, 1780, 3068, 4429
BstV2I GAAGAC 3 cut(s) 2064, 2628, 4240
Bsu15I ATCGAT 1 cut(s) 1008
BsuI GTATCC 2 cut(s) 1905, 2510
BsuRI GGCC 8 cut(s) 55, 569, 1052, 1235, 1877, 2935, 3436, 3543
BsuTUI ATCGAT 1 cut(s) 1008
BtsI GCAGTG 1 cut(s) 1465
BveI ACCTGC 2 cut(s) 783, 3467
Cac8I GCNNGC 7 cut(s) 1996, 2859, 2933, 3061, 3570, 4206, 4301
CaiI CAGNNNCTG 2 cut(s) 1955, 2336
CciI TCATGA 6 cut(s) 793, 892, 1000, 1183, 1366, 3805
CfoI GCGC 2 cut(s) 3366, 3795
Cfr10I RCCGGY 2 cut(s) 2628, 4299
Cfr13I GGNCC 5 cut(s) 165, 1602, 2934, 3444, 3496
ClaI ATCGAT 1 cut(s) 1008
CseI GACGC 1 cut(s) 1942
Csp6I GTAC 7 cut(s) 1944, 2567, 2743, 2908, 2955, 3381, 4166
CspAI ACCGGT 1 cut(s) 2628
CviQI GTAC 7 cut(s) 1944, 2567, 2743, 2908, 2955, 3381, 4166
DdeI CTNAG 9 cut(s) 938, 1764, 1782, 1872, 2337, 2460, 2785, 3658, 3990
DraI TTTAAA 1 cut(s) 3345
DraIII CACNNNGTG 1 cut(s) 2709
DriI GACNNNNNGTC 1 cut(s) 2629
EaeI YGGCCR 2 cut(s) 1050, 1233
Eam1105I GACNNNNNGTC 1 cut(s) 2629
Ecl136II GAGCTC 1 cut(s) 3409
Eco130I CCWWGG 2 cut(s) 3463, 3573
Eco147I AGGCCT 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 3193, 3411
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 4 cut(s) 165, 1602, 3444, 3496
Eco47III AGCGCT 1 cut(s) 3794
Eco53kI GAGCTC 1 cut(s) 3409
Eco57I CTGAAG 7 cut(s) 1932, 2037, 2409, 2633, 2718, 3143, 3807
Eco88I CYCGRG 1 cut(s) 4215
Eco91I GGTNACC 1 cut(s) 1701
EcoICRI GAGCTC 1 cut(s) 3409
EcoO109I RGGNCCY 1 cut(s) 2934
EcoO65I GGTNACC 1 cut(s) 1701
EcoRII CCWGG 8 cut(s) 171, 595, 2250, 2722, 2863, 3128, 3274, 3643
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 2 cut(s) 3463, 3573
EcoT22I ATGCAT 1 cut(s) 2849
EcoT38I GRGCYC 2 cut(s) 3193, 3411
ErhI CCWWGG 2 cut(s) 3463, 3573
Esp3I CGTCTC 1 cut(s) 1925
FalI AAGNNNNNCTT 4 cut(s) 3624, 3656, 4232, 4264
FaqI GGGAC 3 cut(s) 2726, 3989, 4245
FauI CCCGC 1 cut(s) 1396
FauNDI CATATG 5 cut(s) 189, 1846, 2074, 2466, 2522
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 4 cut(s) 811, 1769, 3057, 4418
FriOI GRGCYC 2 cut(s) 3193, 3411
Fsp4HI GCNGC 4 cut(s) 811, 1769, 3057, 4418
FspBI CTAG 3 cut(s) 207, 1539, 2348
GlaI GCGC 2 cut(s) 3365, 3794
GluI GCNGC 4 cut(s) 811, 1769, 3057, 4418
GsaI CCCAGC 2 cut(s) 3537, 4275
GsuI CTGGAG 2 cut(s) 403, 2797
HaeII RGCGCY 1 cut(s) 3796
HaeIII GGCC 8 cut(s) 55, 569, 1052, 1235, 1877, 2935, 3436, 3543
HapII CCGG 5 cut(s) 1605, 2090, 2445, 2629, 4300
HgaI GACGC 1 cut(s) 1942
HhaI GCGC 2 cut(s) 3366, 3795
Hin6I GCGC 2 cut(s) 3364, 3793
HinP1I GCGC 2 cut(s) 3364, 3793
HindIII AAGCTT 1 cut(s) 3638
HpaII CCGG 5 cut(s) 1605, 2090, 2445, 2629, 4300
HphI GGTGA 8 cut(s) 145, 1163, 1346, 2128, 2849, 3442, 3824, 3839
Hpy166II GTNNAC 3 cut(s) 609, 1976, 3613
Hpy8I GTNNAC 3 cut(s) 609, 1976, 3613
HpyCH4III ACNGT 8 cut(s) 128, 613, 1711, 2097, 2223, 2707, 3171, 3421
HpyCH4IV ACGT 3 cut(s) 103, 1591, 2953
HpyF10VI GCNNNNNNNGC 6 cut(s) 1721, 3516, 3525, 3637, 3887, 4109
HpyF3I CTNAG 9 cut(s) 938, 1764, 1782, 1872, 2337, 2460, 2785, 3658, 3990
HpySE526I ACGT 3 cut(s) 103, 1591, 2953
HspAI GCGC 2 cut(s) 3364, 3793
Kpn2I TCCGGA 1 cut(s) 2089
KroI GCCGGC 1 cut(s) 4299
KroNI GCCGGC 1 cut(s) 4301
Ksp22I TGATCA 1 cut(s) 235
LguI GCTCTTC 1 cut(s) 3783
Lsp1109I GCAGC 4 cut(s) 822, 1780, 3068, 4429
LweI GCATC 7 cut(s) 1882, 2554, 2834, 3156, 4033, 4324, 4351
MaeI CTAG 3 cut(s) 207, 1539, 2348
MaeII ACGT 3 cut(s) 103, 1591, 2953
MaeIII GTNAC 8 cut(s) 551, 1701, 2223, 2455, 3323, 3757, 3812, 3827
MhlI GDGCHC 5 cut(s) 1679, 1687, 3193, 3303, 3411
MlyI GAGTC 9 cut(s) 33, 145, 206, 614, 1529, 2426, 2872, 3331, 3882
MmeI TCCRAC 4 cut(s) 192, 1567, 2793, 4129
Mph1103I ATGCAT 1 cut(s) 2849
MroI TCCGGA 1 cut(s) 2089
MroNI GCCGGC 1 cut(s) 4299
MroXI GAANNNNTTC 3 cut(s) 1740, 3261, 3728
MslI CAYNNNNRTG 3 cut(s) 192, 2754, 3810
MspI CCGG 5 cut(s) 1605, 2090, 2445, 2629, 4300
MspR9I CCNGG 9 cut(s) 173, 597, 2252, 2445, 2724, 2865, 3130, 3276, 3645
Mva1269I GAATGC 5 cut(s) 778, 877, 1637, 1861, 3340
MvaI CCWGG 8 cut(s) 173, 597, 2252, 2724, 2865, 3130, 3276, 3645
MwoI GCNNNNNNNGC 6 cut(s) 1721, 3516, 3525, 3637, 3887, 4109
NaeI GCCGGC 1 cut(s) 4301
NciI CCSGG 1 cut(s) 2445
NdeI CATATG 5 cut(s) 189, 1846, 2074, 2466, 2522
NgoMIV GCCGGC 1 cut(s) 4299
NlaIV GGNNCC 7 cut(s) 1558, 1686, 2401, 2936, 3190, 3622, 4187
NmeAIII GCCGAG 1 cut(s) 3691
NmuCI GTSAC 3 cut(s) 3323, 3812, 3827
NsiI ATGCAT 1 cut(s) 2849
NspI RCATGY 2 cut(s) 2861, 3982
NspV TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
PaeI GCATGC 1 cut(s) 2861
PagI TCATGA 6 cut(s) 793, 892, 1000, 1183, 1366, 3805
PceI AGGCCT 1 cut(s) 55
PciSI GCTCTTC 1 cut(s) 3783
PctI GAATGC 5 cut(s) 778, 877, 1637, 1861, 3340
PdiI GCCGGC 1 cut(s) 4301
PdmI GAANNNNTTC 3 cut(s) 1740, 3261, 3728
PflFI GACNNNGTC 1 cut(s) 2375
PinAI ACCGGT 1 cut(s) 2628
PkrI GCNGC 4 cut(s) 812, 1770, 3058, 4419
PleI GAGTC 9 cut(s) 32, 144, 205, 613, 1528, 2426, 2872, 3330, 3881
PpsI GAGTC 9 cut(s) 32, 144, 205, 613, 1528, 2426, 2872, 3330, 3881
Ppu21I YACGTR 1 cut(s) 2954
PshAI GACNNNNGTC 1 cut(s) 3321
PshBI ATTAAT 3 cut(s) 963, 1191, 2043
Psp124BI GAGCTC 1 cut(s) 3411
Psp1406I AACGTT 1 cut(s) 103
Psp6I CCWGG 8 cut(s) 171, 595, 2250, 2722, 2863, 3128, 3274, 3643
PspEI GGTNACC 1 cut(s) 1701
PspFI CCCAGC 2 cut(s) 3533, 4271
PspGI CCWGG 8 cut(s) 171, 595, 2250, 2722, 2863, 3128, 3274, 3643
PspN4I GGNNCC 7 cut(s) 1558, 1686, 2401, 2936, 3190, 3622, 4187
PspPI GGNCC 5 cut(s) 165, 1602, 2934, 3444, 3496
PstNI CAGNNNCTG 2 cut(s) 1955, 2336
PsyI GACNNNGTC 1 cut(s) 2375
RsaI GTAC 7 cut(s) 1945, 2568, 2744, 2909, 2956, 3382, 4167
RsaNI GTAC 7 cut(s) 1944, 2567, 2743, 2908, 2955, 3381, 4166
RseI CAYNNNNRTG 3 cut(s) 192, 2754, 3810
SacI GAGCTC 1 cut(s) 3411
SapI GCTCTTC 1 cut(s) 3783
SatI GCNGC 4 cut(s) 811, 1769, 3057, 4418
Sau96I GGNCC 5 cut(s) 165, 1602, 2934, 3444, 3496
SchI GAGTC 9 cut(s) 33, 145, 206, 614, 1529, 2426, 2872, 3331, 3882
ScrFI CCNGG 9 cut(s) 173, 597, 2252, 2445, 2724, 2865, 3130, 3276, 3645
SduI GDGCHC 5 cut(s) 1679, 1687, 3193, 3303, 3411
SfaNI GCATC 7 cut(s) 1882, 2554, 2834, 3156, 4033, 4324, 4351
SfcI CTRYAG 2 cut(s) 1707, 3671
SfuI TTCGAA 4 cut(s) 1133, 1316, 3237, 4346
SinI GGWCC 4 cut(s) 165, 1602, 3444, 3496
SmiMI CAYNNNNRTG 3 cut(s) 192, 2754, 3810
SmlI CTYRAG 2 cut(s) 279, 4430
SmoI CTYRAG 2 cut(s) 279, 4430
SphI GCATGC 1 cut(s) 2861
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 6 cut(s) 320, 1403, 1644, 3424, 3551, 4382
SspMI CTAG 3 cut(s) 207, 1539, 2348
SstI GAGCTC 1 cut(s) 3411
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 9 cut(s) 171, 595, 2250, 2443, 2722, 2863, 3128, 3274, 3643
StyI CCWWGG 2 cut(s) 3463, 3573
TaaI ACNGT 8 cut(s) 128, 613, 1711, 2097, 2223, 2707, 3171, 3421
TaiI ACGT 3 cut(s) 106, 1594, 2956
TatI WGTACW 1 cut(s) 4165
TseFI GTSAC 3 cut(s) 3323, 3812, 3827
TseI GCWGC 4 cut(s) 810, 1768, 3056, 4417
Tsp45I GTSAC 3 cut(s) 3323, 3812, 3827
TspGWI ACGGA 1 cut(s) 1708
Tth111I GACNNNGTC 1 cut(s) 2375
VpaK11BI GGWCC 4 cut(s) 165, 1602, 3444, 3496
VspI ATTAAT 3 cut(s) 963, 1191, 2043
XapI RAATTY 5 cut(s) 1832, 2768, 2914, 3269, 3501
XceI RCATGY 2 cut(s) 2861, 3982
XmnI GAANNNNTTC 3 cut(s) 1740, 3261, 3728
XspI CTAG 3 cut(s) 207, 1539, 2348
Zsp2I ATGCAT 1 cut(s) 2849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.