FvH4_5g29000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000939
Physical Location & Seq
Forward (+)
20129671 .. 20136369
6699 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29000.t4

Sequence Viewer

Length: 924 bp
ATGGCAATGGCTTCTCTTCAACCTTCATGGCTCTCTTCTCTCGGTCTCTTTCCCTCCTCAAACCCCCACCATTCGAAGTCATTCAAGCTCTTCTCCTCTCTCAACTCACCAAATCCACCCGAAACGACGACGCCCCCAGTCGACCCTGCCAAGTCGTATAAGAAGAACCCAAGTCCCGCGAACAGGGAATTGCCTCCTGCTCTTAAGACTGCAATGGAGAGAGCAAAAGAGTATAAGCAAAAGAAGAACAACGTTACTAACACAAATCCAGGTGGTGAGAGTGTCAAGGGGAAATTGTCAGTGTCGAGCATGGATTTTGTGGGACTTGGGTTTGCGGATAAGAAGGAGGGTAGGGCAGTGCCAGCTGGATTGGTTCCATTTACAGACGATTTTGCGGAGGGGGACAAGTTGCCTGAGGTTGAGATTATTGTTGGGGACTCTAGTAATTTCGGTGCAAGGACAAGCAAGCCTGAGCAACAGGCTCAAGGAGATGACCCGGATCTTTACAAGCCCAGGGTTTCCTCATGGGGGGTGTTTCCCAGACCTAATGACATTTCGAAAACGTTTGGTGGTGGGCGAGTTATACGCCCCGGGGAAGTGCTTGAAACAGAGGAAGAAAAAGCTGCTAAAGAGGCACGAACAAGACAATTAGTTGCTGCTTACAAGAGTAAAATGGGCTTGAACATTGATCCAAAGCTCAGACTGGACTGTGAGAAGACATTAAAGGATGGTGACTCATTGATGAACGTTGGAAAGCTAAAGGAAGCATTACCCTACTATGAGAAAGTTATGGACAAGTTACCGTTTAAGAGTGATCTTCATGGGCTTGCTGCTTTACAGTGGTCTATTTGTCAAGACTCCCTGAGACAAAAGAAGCTCGAAATATGTATGAAAGGCTTCAGTCCCACCCAACTGCTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

33.73

Weight (kDa)

9.0

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 179
AciI CCGC 3 cut(s) 177, 335, 395
AclI AACGTT 3 cut(s) 252, 563, 747
AclWI GGATC 2 cut(s) 507, 683
AcuI CTGAAG 1 cut(s) 883
AcyI GRCGYC 1 cut(s) 131
AfiI CCNNNNNNNGG 2 cut(s) 183, 528
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 4 cut(s) 20, 85, 605, 682
AjnI CCWGG 2 cut(s) 268, 512
AluBI AGCT 6 cut(s) 88, 365, 623, 697, 757, 877
AluI AGCT 6 cut(s) 88, 365, 623, 697, 757, 877
Alw26I GTCTC 2 cut(s) 50, 859
AlwI GGATC 2 cut(s) 507, 683
Ama87I CYCGRG 1 cut(s) 590
ApeKI GCWGC 3 cut(s) 623, 656, 830
Asp700I GAANNNNTTC 2 cut(s) 80, 896
AsuC2I CCSGG 3 cut(s) 497, 591, 592
AsuHPI GGTGA 3 cut(s) 99, 287, 743
AsuII TTCGAA 2 cut(s) 74, 557
AvaI CYCGRG 1 cut(s) 590
AxyI CCTNAGG 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 722
BbvI GCAGC 3 cut(s) 610, 643, 817
BccI CCATC 1 cut(s) 722
BciT130I CCWGG 2 cut(s) 270, 514
BcnI CCSGG 3 cut(s) 497, 591, 592
BcoDI GTCTC 2 cut(s) 50, 859
BfaI CTAG 2 cut(s) 441, 917
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 3 cut(s) 624, 657, 831
BlsI GCNGC 3 cut(s) 625, 658, 832
Bme1390I CCNGG 5 cut(s) 270, 497, 514, 591, 592
BmeT110I CYCGRG 1 cut(s) 590
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 5 cut(s) 270, 497, 514, 591, 592
BmrI ACTGGG 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 722
Bpu10I CCTNAGC 1 cut(s) 471
Bpu14I TTCGAA 2 cut(s) 74, 557
BpuEI CTTGAG 1 cut(s) 468
BpuMI CCSGG 3 cut(s) 497, 591, 592
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 5 cut(s) 512, 513, 589, 590, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 528
Bse1I ACTGG 2 cut(s) 137, 708
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 12, 219
BseBI CCWGG 2 cut(s) 270, 514
BseDI CCNNGG 5 cut(s) 512, 513, 589, 590, 591
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 2 cut(s) 183, 528
BseMI GCAATG 2 cut(s) 12, 219
BseMII CTCAG 4 cut(s) 405, 462, 712, 854
BseNI ACTGG 2 cut(s) 137, 708
BseRI GAGGAG 2 cut(s) 46, 85
BseXI GCAGC 3 cut(s) 610, 643, 817
Bsh1236I CGCG 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 2 cut(s) 497, 591
BslFI GGGAC 5 cut(s) 159, 336, 416, 449, 888
BslI CCNNNNNNNGG 2 cut(s) 183, 528
BsmAI GTCTC 2 cut(s) 50, 859
BsmFI GGGAC 5 cut(s) 159, 336, 416, 449, 888
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 590
Bsp119I TTCGAA 2 cut(s) 74, 557
Bsp143I GATC 3 cut(s) 499, 688, 814
BspACI CCGC 3 cut(s) 177, 335, 395
BspCNI CTCAG 4 cut(s) 406, 463, 711, 855
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 1 cut(s) 375
BspPI GGATC 2 cut(s) 507, 683
BspQI GCTCTTC 1 cut(s) 95
BspT104I TTCGAA 2 cut(s) 74, 557
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 2 cut(s) 12, 219
BsrI ACTGG 2 cut(s) 137, 708
BssECI CCNNGG 5 cut(s) 512, 513, 589, 590, 591
BssMI GATC 3 cut(s) 499, 688, 814
BssNI GRCGYC 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 270, 514
Bst4CI ACNGT 3 cut(s) 710, 804, 840
Bst6I CTCTTC 3 cut(s) 21, 40, 95
BstACI GRCGYC 1 cut(s) 131
BstAFI CTTAAG 1 cut(s) 203
BstBI TTCGAA 2 cut(s) 74, 557
BstC8I GCNNGC 3 cut(s) 363, 467, 828
BstDEI CTNAG 4 cut(s) 414, 471, 698, 863
BstF5I GGATG 1 cut(s) 733
BstFNI CGCG 1 cut(s) 179
BstKTI GATC 3 cut(s) 502, 691, 817
BstMAI GTCTC 2 cut(s) 50, 859
BstMBI GATC 3 cut(s) 499, 688, 814
BstMWI GCNNNNNNNGC 2 cut(s) 362, 632
BstNI CCWGG 2 cut(s) 270, 514
BstSCI CCNGG 5 cut(s) 268, 495, 512, 589, 590
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 610, 643, 817
BstV2I GAAGAC 1 cut(s) 722
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
Bsu36I CCTNAGG 1 cut(s) 414
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 306, 363, 845
Cac8I GCNNGC 3 cut(s) 363, 467, 828
Cfr9I CCCGGG 1 cut(s) 590
CseI GACGC 1 cut(s) 139
CviAII CATG 4 cut(s) 27, 310, 525, 821
DdeI CTNAG 4 cut(s) 414, 471, 698, 863
DpnI GATC 3 cut(s) 501, 690, 816
DpnII GATC 3 cut(s) 499, 688, 814
Eam1104I CTCTTC 3 cut(s) 21, 40, 95
EarI CTCTTC 3 cut(s) 21, 40, 95
Eco31I GGTCTC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 883
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 268, 512
FaeI CATG 4 cut(s) 30, 313, 528, 824
FaqI GGGAC 5 cut(s) 159, 336, 416, 449, 888
FatI CATG 4 cut(s) 26, 309, 524, 820
FauI CCCGC 1 cut(s) 184
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 3 cut(s) 624, 657, 831
FokI GGATG 1 cut(s) 740
Fsp4HI GCNGC 3 cut(s) 624, 657, 831
FspBI CTAG 2 cut(s) 441, 917
GluI GCNGC 3 cut(s) 624, 657, 831
HapII CCGG 2 cut(s) 497, 591
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 4 cut(s) 30, 313, 528, 824
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 3 cut(s) 437, 734, 857
HpaII CCGG 2 cut(s) 497, 591
HphI GGTGA 3 cut(s) 99, 287, 743
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 1 cut(s) 701
Hpy188III TCNNGA 1 cut(s) 854
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 2 cut(s) 130, 133
HpyAV CCTTC 2 cut(s) 33, 337
HpyCH4III ACNGT 3 cut(s) 710, 804, 840
HpyCH4IV ACGT 3 cut(s) 252, 563, 747
HpyCH4V TGCA 2 cut(s) 212, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 632
HpyF3I CTNAG 4 cut(s) 414, 471, 698, 863
HpySE526I ACGT 3 cut(s) 252, 563, 747
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 4 cut(s) 30, 313, 528, 824
Kzo9I GATC 3 cut(s) 499, 688, 814
LguI GCTCTTC 1 cut(s) 95
Lsp1109I GCAGC 3 cut(s) 610, 643, 817
MaeI CTAG 2 cut(s) 441, 917
MaeII ACGT 3 cut(s) 252, 563, 747
MaeIII GTNAC 3 cut(s) 253, 731, 798
MalI GATC 3 cut(s) 501, 690, 816
MboI GATC 3 cut(s) 499, 688, 814
MboII GAAGA 8 cut(s) 8, 27, 82, 175, 256, 626, 727, 809
MflI RGATCY 1 cut(s) 499
MluCI AATT 4 cut(s) 188, 293, 445, 647
MlyI GAGTC 3 cut(s) 431, 728, 851
MmeI TCCRAC 1 cut(s) 730
MroXI GAANNNNTTC 2 cut(s) 80, 896
MseI TTAA 3 cut(s) 204, 722, 807
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 2 cut(s) 497, 591
MspR9I CCNGG 5 cut(s) 270, 497, 514, 591, 592
MvaI CCWGG 2 cut(s) 270, 514
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 362, 632
NciI CCSGG 3 cut(s) 497, 591, 592
NdeII GATC 3 cut(s) 499, 688, 814
NlaIII CATG 4 cut(s) 30, 313, 528, 824
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 731
NspV TTCGAA 2 cut(s) 74, 557
PasI CCCWGGG 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 95
PdmI GAANNNNTTC 2 cut(s) 80, 896
PkrI GCNGC 3 cut(s) 625, 658, 832
PleI GAGTC 3 cut(s) 431, 728, 851
PpsI GAGTC 3 cut(s) 431, 728, 851
Psp1406I AACGTT 3 cut(s) 252, 563, 747
Psp6I CCWGG 2 cut(s) 268, 512
PspGI CCWGG 2 cut(s) 268, 512
PspN4I GGNNCC 1 cut(s) 375
PsuI RGATCY 1 cut(s) 499
PvuII CAGCTG 1 cut(s) 365
SalI GTCGAC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 204, 722, 807
SatI GCNGC 3 cut(s) 624, 657, 831
Sau3AI GATC 3 cut(s) 499, 688, 814
SchI GAGTC 3 cut(s) 431, 728, 851
ScrFI CCNGG 5 cut(s) 270, 497, 514, 591, 592
SfuI TTCGAA 2 cut(s) 74, 557
SmaI CCCGGG 1 cut(s) 592
SmlI CTYRAG 2 cut(s) 203, 483
SmoI CTYRAG 2 cut(s) 203, 483
Sse9I AATT 4 cut(s) 188, 293, 445, 647
SsiI CCGC 3 cut(s) 177, 335, 395
SspMI CTAG 2 cut(s) 441, 917
StyD4I CCNGG 5 cut(s) 268, 495, 512, 589, 590
TaaI ACNGT 3 cut(s) 710, 804, 840
TaiI ACGT 3 cut(s) 255, 566, 750
TaqI TCGA 5 cut(s) 74, 141, 305, 557, 879
TaqII GACCGA 1 cut(s) 32
TasI AATT 4 cut(s) 188, 293, 445, 647
Tru1I TTAA 3 cut(s) 204, 722, 807
Tru9I TTAA 3 cut(s) 204, 722, 807
TscAI CASTG 3 cut(s) 306, 363, 845
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 3 cut(s) 623, 656, 830
Tsp45I GTSAC 1 cut(s) 731
TspDTI ATGAA 4 cut(s) 15, 758, 809, 905
TspMI CCCGGG 1 cut(s) 590
TspRI CASTG 3 cut(s) 306, 363, 845
Vha464I CTTAAG 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 590
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 2 cut(s) 80, 896
XspI CTAG 2 cut(s) 441, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.