Rmu_sc0003745.1_g000009

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003745.1
OG0000939
Physical Location & Seq
Forward (+)
50152 .. 51848
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003745.1_g000009.1.cds

Sequence Viewer

Length: 543 bp
atgggccaggaacttgaagaacaagcagcgctgccggtcatcctccgatggcaagatttgggcgagttgccggaaaacgagttgattattaaggctcctgaagacaatacccctcccttcaaagctcttcttttctctcaacccacccaatcccaatgccgaatccgaaaccacgacgtccccagtgttgaagcaaatgagaaaaacaagggaaaattgtcagtgtcgagcatggattttgtgggacttgggtttgctgataagaatgagggtagagcactgccagctgggttggttccaatcgcatacgattttgctcagggcaacttgcctgaggttgagattattgttggggactccagtaatttcggtgcagccacacccaagcctcaaggagatgaccccctggatctttacaagccaaagacattaaaggatggtgactcattgatgaacgtcggaaagctgaaggaagcattaccctactatgagaaagttatggataagttaccattcaaggtaatgcttcttgcacatgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.77

Weight (kDa)

4.82

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AclWI GGATC 1 cut(s) 417
AcuI CTGAAG 2 cut(s) 120, 488
AcyI GRCGYC 1 cut(s) 177
AfeI AGCGCT 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 4 cut(s) 17, 121, 191, 517
AjnI CCWGG 2 cut(s) 6, 405
AluBI AGCT 3 cut(s) 125, 287, 466
AluI AGCT 3 cut(s) 125, 287, 466
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 1 cut(s) 417
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 1 cut(s) 4
ApeKI GCWGC 3 cut(s) 26, 31, 374
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 452
AxyI CCTNAGG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 3 cut(s) 18, 38, 386
BccI CCATC 2 cut(s) 42, 431
BciT130I CCWGG 2 cut(s) 8, 407
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 3 cut(s) 27, 32, 375
BlsI GCNGC 3 cut(s) 28, 33, 376
Bme1390I CCNGG 2 cut(s) 8, 407
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 96, 297
BmrFI CCNGG 2 cut(s) 8, 407
BmrI ACTGGG 1 cut(s) 177
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 343
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 405
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 183, 360
Bse21I CCTNAGG 1 cut(s) 333
BseBI CCWGG 2 cut(s) 8, 407
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 2 cut(s) 39, 442
BseMII CTCAG 2 cut(s) 324, 332
BseNI ACTGG 2 cut(s) 183, 360
BseXI GCAGC 3 cut(s) 18, 38, 386
BseYI CCCAGC 1 cut(s) 287
BsgI GTGCAG 1 cut(s) 393
BshFI GGCC 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 2 cut(s) 35, 71
BslFI GGGAC 3 cut(s) 164, 258, 368
BsmFI GGGAC 3 cut(s) 164, 258, 368
BsnI GGCC 1 cut(s) 6
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 1 cut(s) 409
BspANI GGCC 1 cut(s) 6
BspCNI CTCAG 2 cut(s) 325, 331
BspLI GGNNCC 2 cut(s) 96, 297
BspPI GGATC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 132
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 2 cut(s) 183, 360
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 1 cut(s) 409
BssNI GRCGYC 1 cut(s) 177
Bst2UI CCWGG 2 cut(s) 8, 407
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 318, 333
BstF5I GGATG 2 cut(s) 39, 442
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 1 cut(s) 412
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 2 cut(s) 8, 407
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 6, 405
BstV1I GCAGC 3 cut(s) 18, 38, 386
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
Bsu36I CCTNAGG 1 cut(s) 333
BsuRI GGCC 1 cut(s) 6
BtsCI GGATG 2 cut(s) 39, 442
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 3 cut(s) 190, 228, 278
Cac8I GCNNGC 1 cut(s) 285
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 2 cut(s) 232, 536
CviJI RGCY 8 cut(s) 6, 95, 125, 287, 377, 388, 421, 466
CviKI_1 RGCY 8 cut(s) 6, 95, 125, 287, 377, 388, 421, 466
DdeI CTNAG 2 cut(s) 318, 333
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco47III AGCGCT 1 cut(s) 30
Eco57I CTGAAG 2 cut(s) 120, 488
Eco81I CCTNAGG 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 6, 405
FaeI CATG 2 cut(s) 235, 539
FaiI YATR 5 cut(s) 233, 307, 489, 500, 537
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 3 cut(s) 164, 258, 368
FatI CATG 2 cut(s) 231, 535
Fnu4HI GCNGC 3 cut(s) 27, 32, 375
FokI GGATG 2 cut(s) 26, 449
Fsp4HI GCNGC 3 cut(s) 27, 32, 375
GlaI GCGC 1 cut(s) 30
GluI GCNGC 3 cut(s) 27, 32, 375
GsaI CCCAGC 1 cut(s) 291
GsuI CTGGAG 1 cut(s) 343
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 6
HapII CCGG 2 cut(s) 35, 71
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 2 cut(s) 235, 539
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HinfI GANTC 3 cut(s) 162, 356, 443
HpaII CCGG 2 cut(s) 35, 71
HphI GGTGA 1 cut(s) 452
Hpy188I TCNGA 3 cut(s) 47, 167, 461
Hpy188III TCNNGA 1 cut(s) 98
Hpy99I CGWCG 2 cut(s) 179, 461
HpyAV CCTTC 2 cut(s) 127, 463
HpyCH4IV ACGT 2 cut(s) 177, 456
HpyCH4V TGCA 2 cut(s) 374, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpyF3I CTNAG 2 cut(s) 318, 333
HpySE526I ACGT 2 cut(s) 177, 456
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 2 cut(s) 235, 539
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 1 cut(s) 409
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 3 cut(s) 18, 38, 386
MaeII ACGT 2 cut(s) 177, 456
MaeIII GTNAC 2 cut(s) 440, 507
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 3 cut(s) 29, 113, 119
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 2 cut(s) 215, 364
MlyI GAGTC 2 cut(s) 350, 437
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 5 cut(s) 53, 123, 262, 328, 399
MseI TTAA 3 cut(s) 90, 431, 541
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 35, 71
MspR9I CCNGG 2 cut(s) 8, 407
MvaI CCWGG 2 cut(s) 8, 407
MwoI GCNNNNNNNGC 1 cut(s) 284
NdeII GATC 1 cut(s) 409
NlaIII CATG 2 cut(s) 235, 539
NlaIV GGNNCC 2 cut(s) 96, 297
NmuCI GTSAC 1 cut(s) 440
NspI RCATGY 1 cut(s) 539
PciI ACATGT 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 28, 33, 376
PleI GAGTC 2 cut(s) 350, 437
PpsI GAGTC 2 cut(s) 350, 437
PscI ACATGT 1 cut(s) 535
Psp6I CCWGG 2 cut(s) 6, 405
PspFI CCCAGC 1 cut(s) 287
PspGI CCWGG 2 cut(s) 6, 405
PspN4I GGNNCC 2 cut(s) 96, 297
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 3 cut(s) 90, 431, 541
SatI GCNGC 3 cut(s) 27, 32, 375
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 350, 437
ScrFI CCNGG 2 cut(s) 8, 407
SduI GDGCHC 1 cut(s) 280
SetI ASST 7 cut(s) 127, 180, 289, 339, 459, 468, 522
SmlI CTYRAG 1 cut(s) 390
SmoI CTYRAG 1 cut(s) 390
Sse9I AATT 2 cut(s) 215, 364
StyD4I CCNGG 2 cut(s) 6, 405
TaiI ACGT 2 cut(s) 180, 459
TaqI TCGA 1 cut(s) 227
TasI AATT 2 cut(s) 215, 364
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 3 cut(s) 90, 431, 541
Tru9I TTAA 3 cut(s) 90, 431, 541
TscAI CASTG 3 cut(s) 190, 228, 285
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 3 cut(s) 26, 31, 374
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 1 cut(s) 467
TspRI CASTG 3 cut(s) 190, 228, 285
XceI RCATGY 1 cut(s) 539
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.