Rh2CG512600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000939
Physical Location & Seq
Forward (+)
68660717 .. 68663512
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG512600.1

Sequence Viewer

Length: 735 bp
ATGGAGAGAGCTAGAGAGTATAAGCAGAAGAAGACAGGGACTAGCAAAACTTCAGGTGTTGAAGCAAATGAGAAAAACAAGGGAAAATTGTCAGTGTCGAGCATGGATTTTGTGGGACTTGGGTTTGCTGATAAGAAGGAGGGTAAAGCACTGCCAGCTGGGTTGGTTCCAATCGCAGACGATTTTGCTCAGGGCAACTTGCCTGAGGTTGAGATTATTGTTGGGGACTCCAGTAATTTCGATGCAGCCACACCCAAGCCTCAAGGAGATGACCCCTTGGATCTTTACAAGCCAAAGGTTTCTTCATGGGGAGTCTTTCCCAGACCCAATGACATTTCAAAAATGTTTGGTGGTGGGCGAGTTATACACCCCGGGGAAGTGCTTGAAACGCAGGAAGAAAAAGCTGCTAAGGAGGAACGAACAAGACAATTAGTTGCTGCTTACAACACTAAAATGGGCTTGAACATTGATCCCAAGCTCAGATTGGACTGTGAGAAGACATTAAAGGATGGTGACTCATTGATGAACGTCGGAAAGCTGAAGGAAGCATTACCCTACTATGAGAAAGTTATGGATAAGTTACCATTCAAGATTATGACTCCGAAAGCTCGTGCAGACATACACATCAATCTTCCAGCACTTAAGAAGTTGGACTTGATGCTTATTGAAACACTTGATTCAATGGTGGATACTGAGTTTGGGTATGTAGAAGGTGGTAGTAGGGCAGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.81

Weight (kDa)

5.89

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 180 - 242 1.8e-17 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 288, 464
AcuI CTGAAG 2 cut(s) 36, 560
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 7 cut(s) 62, 339, 386, 463, 589, 668, 681
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AluBI AGCT 6 cut(s) 11, 158, 404, 478, 538, 608
AluI AGCT 6 cut(s) 11, 158, 404, 478, 538, 608
AlwI GGATC 2 cut(s) 288, 464
Ama87I CYCGRG 1 cut(s) 371
ApeKI GCWGC 3 cut(s) 245, 404, 437
AsuC2I CCSGG 2 cut(s) 372, 373
AsuHPI GGTGA 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 204
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 2 cut(s) 38, 503
BbvI GCAGC 3 cut(s) 257, 391, 424
BccI CCATC 1 cut(s) 503
BciVI GTATCC 1 cut(s) 682
BcnI CCSGG 2 cut(s) 372, 373
BfaI CTAG 2 cut(s) 12, 42
BfrI CTTAAG 1 cut(s) 641
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 3 cut(s) 246, 405, 438
BlsI GCNGC 3 cut(s) 247, 406, 439
Bme1390I CCNGG 2 cut(s) 372, 373
BmeT110I CYCGRG 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 2 cut(s) 372, 373
BmsI GCATC 2 cut(s) 232, 648
BpiI GAAGAC 2 cut(s) 38, 503
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 2 cut(s) 189, 408
BpuEI CTTGAG 1 cut(s) 246
BpuMI CCSGG 2 cut(s) 372, 373
BsaJI CCNNGG 4 cut(s) 276, 370, 371, 372
Bse1I ACTGG 1 cut(s) 231
Bse21I CCTNAGG 1 cut(s) 204
BseDI CCNNGG 4 cut(s) 276, 370, 371, 372
BseGI GGATG 1 cut(s) 514
BseMII CTCAG 4 cut(s) 195, 203, 493, 684
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 257, 391, 424
BseYI CCCAGC 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 3 cut(s) 52, 129, 239
BsmFI GGGAC 3 cut(s) 52, 129, 239
BsoBI CYCGRG 1 cut(s) 371
Bsp143I GATC 2 cut(s) 280, 469
BspCNI CTCAG 4 cut(s) 196, 202, 492, 685
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 2 cut(s) 288, 464
BspTI CTTAAG 1 cut(s) 641
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 4 cut(s) 276, 370, 371, 372
BssMI GATC 2 cut(s) 280, 469
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 609
Bst4CI ACNGT 1 cut(s) 491
BstAFI CTTAAG 1 cut(s) 641
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 5 cut(s) 189, 204, 408, 479, 693
BstF5I GGATG 1 cut(s) 514
BstKTI GATC 2 cut(s) 283, 472
BstMBI GATC 2 cut(s) 280, 469
BstMWI GCNNNNNNNGC 2 cut(s) 155, 388
BstSCI CCNGG 2 cut(s) 370, 371
BstV1I GCAGC 3 cut(s) 257, 391, 424
BstV2I GAAGAC 2 cut(s) 38, 503
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 1 cut(s) 682
BtsCI GGATG 1 cut(s) 514
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 2 cut(s) 99, 149
Cac8I GCNNGC 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 371
CviAII CATG 2 cut(s) 103, 306
DdeI CTNAG 5 cut(s) 189, 204, 408, 479, 693
DpnI GATC 2 cut(s) 282, 471
DpnII GATC 2 cut(s) 280, 469
Eco130I CCWWGG 1 cut(s) 276
Eco57I CTGAAG 2 cut(s) 36, 560
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 2 cut(s) 106, 309
FaiI YATR 9 cut(s) 21, 104, 307, 365, 561, 572, 596, 620, 705
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 3 cut(s) 52, 129, 239
FatI CATG 2 cut(s) 102, 305
Fnu4HI GCNGC 3 cut(s) 246, 405, 438
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 3 cut(s) 246, 405, 438
FspBI CTAG 2 cut(s) 12, 42
GluI GCNGC 3 cut(s) 246, 405, 438
GsaI CCCAGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 214
HapII CCGG 1 cut(s) 372
Hin1II CATG 2 cut(s) 106, 309
HinfI GANTC 5 cut(s) 227, 312, 515, 598, 677
HpaII CCGG 1 cut(s) 372
HphI GGTGA 1 cut(s) 524
Hpy188I TCNGA 3 cut(s) 482, 533, 603
Hpy188III TCNNGA 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 4 cut(s) 130, 535, 704, 722
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 2 cut(s) 245, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 388
HpyF3I CTNAG 5 cut(s) 189, 204, 408, 479, 693
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 2 cut(s) 106, 309
Kzo9I GATC 2 cut(s) 280, 469
Lsp1109I GCAGC 3 cut(s) 257, 391, 424
LweI GCATC 2 cut(s) 232, 648
MaeI CTAG 2 cut(s) 12, 42
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 2 cut(s) 512, 579
MalI GATC 2 cut(s) 282, 471
MboI GATC 2 cut(s) 280, 469
MboII GAAGA 6 cut(s) 40, 43, 294, 407, 508, 623
MflI RGATCY 1 cut(s) 280
MluCI AATT 3 cut(s) 86, 235, 428
MlyI GAGTC 4 cut(s) 221, 321, 509, 592
MmeI TCCRAC 2 cut(s) 511, 630
MnlI CCTC 4 cut(s) 133, 199, 270, 406
MseI TTAA 2 cut(s) 503, 642
MslI CAYNNNNRTG 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 158
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 372, 373
MwoI GCNNNNNNNGC 2 cut(s) 155, 388
NciI CCSGG 2 cut(s) 372, 373
NdeII GATC 2 cut(s) 280, 469
NlaIII CATG 2 cut(s) 106, 309
NlaIV GGNNCC 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 512
PfeI GAWTC 1 cut(s) 677
PkrI GCNGC 3 cut(s) 247, 406, 439
PleI GAGTC 4 cut(s) 221, 320, 509, 592
PpsI GAGTC 4 cut(s) 221, 320, 509, 592
PspFI CCCAGC 1 cut(s) 158
PspN4I GGNNCC 1 cut(s) 168
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 452
SaqAI TTAA 2 cut(s) 503, 642
SatI GCNGC 3 cut(s) 246, 405, 438
Sau3AI GATC 2 cut(s) 280, 469
SchI GAGTC 4 cut(s) 221, 321, 509, 592
ScrFI CCNGG 2 cut(s) 372, 373
SfaNI GCATC 2 cut(s) 232, 648
SmaI CCCGGG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 2 cut(s) 261, 641
SmoI CTYRAG 2 cut(s) 261, 641
Sse9I AATT 3 cut(s) 86, 235, 428
SspMI CTAG 2 cut(s) 12, 42
StyD4I CCNGG 2 cut(s) 370, 371
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 1 cut(s) 531
TaqI TCGA 2 cut(s) 98, 240
TasI AATT 3 cut(s) 86, 235, 428
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 2 cut(s) 503, 642
Tru9I TTAA 2 cut(s) 503, 642
TscAI CASTG 2 cut(s) 99, 156
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 3 cut(s) 245, 404, 437
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 2 cut(s) 294, 539
TspMI CCCGGG 1 cut(s) 371
TspRI CASTG 2 cut(s) 99, 156
Vha464I CTTAAG 1 cut(s) 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 481
XmaI CCCGGG 1 cut(s) 371
XspI CTAG 2 cut(s) 12, 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.