pycom02g24770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000939
Physical Location & Seq
Forward (+)
22869722 .. 22874203
4482 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24770.4

Sequence Viewer

Length: 1419 bp
ATGGCTTCCCTTCAACCTTCATTGTTCTCTTCCCTCAGCACCATCCCTTCCACCAAACCCACTCTCTTCCTCTCCATAAACCCTAACAAACCCCACTCTTCAAAACCATTCAAATTCTCCTTCTCCCTGAACCCACCCAACGCTGAATCTTCACAACCCACCCCGCCAAATTCACCCGAAACGACGCCGGAAGCCGAGCCGGGTCCCGTTGACCCTGTCAAGCTCGCATTGGAGAACGCCAAGGCTTACAAGAAGTCGGTGCAGATGAACAAGAATTCGAAAATTGAGAAAAACCCTGCTGAGGATAGTGGTGGAATTGCTGGAAATGGTGGGACGGGTGCGGGTTCGGGTTCGGATGGTGGGGAGAAAGAAATGCCTGCTTCGGTTAAGATTGCAATGGAGAAAGCTAAAGAGTATAAGAAGAGTAAAGGCGCTGTGGGTGGTGGTGACATTAATGCTGGGGAGAACAAAGGCACAATTTCAGGTTTGAAGGGAAGTGCTGGCGGAAATTCGGGGAAAGAGGTAGTGGAAAAGAAGGCGAAATTGTCAGTTTCAAGCATTGATTTTGTGGGACTCGGCTTTGCGGATAAGAAGGAGGGCAGAGGACTGCCCGCTGGGTTAGTTCCAATATCGGACTATATTCCAGAAGGGGGCTCTCCTGATGTCGAGTTTATAGCAGGGGACACTAGAAATTTCAATGCAGGGCCGCGGCAGACAGAGCAGACTCAAGGAGATAATCCAGACCTGTACAAGCCAAAAGTTTCTTCGTGGGGAGTATTTCCTAGACCGAACGACATTTCCAAAACGTTTGGAGGTGGAAGAGTTATACGCCCAGGGCAAGTGCTGGAAACAGCTGAAGAAAAAGCAGCTAAAGAAGCACGCACAAGACAATTAGTAGCTGCTTACAAGAGTAAAATGGGGTTGAACATTGATCCAAAGCTAAGAGCTGAATGTGAGAAGGCGTTAAAGGATGGCGACTCATTGATGAATGTTGGAGAGCTTAAGGAAGCATTAATCTATTATCAGAAGGTTATGGATAAGTTACCATTCAAGAGTGAGCTTCATGGGTTGGCTGCTTTACAATGGTCCATTTGTCTGGACTCCCTCAGCAGGTCAAAGGAAGCTCAAGTTATGTACGAGAAGCTTCAGTCCCACCCAACTCCTAGAGTAAGTAAGAAGGCGAGGCAATTTGTGTTCAGCTTTCAGGCCATGGAAATGTTGAGGCTTACAAAGAACTCACCTTTGAAGAACACTGGCTTCCAGAATTATTTTGAAGCTTTCATTGAAAACAAATCGGACTACGTGCTAAAAGAGGATGAAGTCGAAGTAGGTACACTGAATCAAACTCTACCATATATCATTTTTCTTGTTTCTCCCATCCTTTTGGTGCTACTCATTGCTATACAAAACAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

473

Amino Acids

50.94

Weight (kDa)

9.05

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1101
AccII CGCG 1 cut(s) 709
AciI CCGC 7 cut(s) 164, 341, 504, 584, 612, 707, 709
AclI AACGTT 1 cut(s) 806
AclWI GGATC 1 cut(s) 926
AcsI RAATTY 5 cut(s) 113, 169, 274, 508, 691
AcuI CTGAAG 2 cut(s) 876, 1130
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 3 cut(s) 749, 1136, 1333
AfiI CCNNNNNNNGG 3 cut(s) 301, 650, 1110
AflII CTTAAG 1 cut(s) 1001
AjnI CCWGG 1 cut(s) 832
AjuI GAANNNNNNNTTGG 2 cut(s) 748, 780
AloI GAACNNNNNNTCC 2 cut(s) 782, 814
AlwI GGATC 1 cut(s) 926
AoxI GGCC 2 cut(s) 704, 1206
ApeKI GCWGC 3 cut(s) 866, 899, 1073
ApoI RAATTY 5 cut(s) 113, 169, 274, 508, 691
AseI ATTAAT 2 cut(s) 453, 1013
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 3 cut(s) 203, 704, 1086
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 3 cut(s) 165, 458, 1230
AsuII TTCGAA 1 cut(s) 278
AvaII GGWCC 2 cut(s) 203, 1086
BanII GRGCYC 1 cut(s) 656
BbvCI CCTCAGC 3 cut(s) 35, 300, 1106
BbvI GCAGC 3 cut(s) 878, 886, 1060
BccI CCATC 4 cut(s) 50, 350, 965, 1385
BciT130I CCWGG 1 cut(s) 834
BcnI CCSGG 1 cut(s) 201
BfaI CTAG 3 cut(s) 687, 783, 1164
BfoI RGCGCY 1 cut(s) 435
BfrI CTTAAG 1 cut(s) 1001
BfuAI ACCTGC 1 cut(s) 1101
BisI GCNGC 5 cut(s) 707, 710, 867, 900, 1074
BlsI GCNGC 5 cut(s) 708, 711, 868, 901, 1075
Bme1390I CCNGG 2 cut(s) 201, 834
Bme18I GGWCC 2 cut(s) 203, 1086
BmgT120I GGNCC 3 cut(s) 203, 704, 1086
BmiI GGNNCC 2 cut(s) 204, 205
BmrFI CCNGG 2 cut(s) 201, 834
Bpu10I CCTNAGC 3 cut(s) 35, 300, 1106
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 2 cut(s) 711, 1110
BpuMI CCSGG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 1303
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 5 cut(s) 240, 707, 832, 833, 1209
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 650, 1110
Bse1I ACTGG 1 cut(s) 1258
Bse3DI GCAATG 2 cut(s) 402, 1395
BseBI CCWGG 1 cut(s) 834
BseDI CCNNGG 5 cut(s) 240, 707, 832, 833, 1209
BseGI GGATG 5 cut(s) 42, 361, 976, 1321, 1377
BseLI CCNNNNNNNGG 3 cut(s) 301, 650, 1110
BseMI GCAATG 2 cut(s) 402, 1395
BseMII CTCAG 3 cut(s) 49, 291, 1120
BseNI ACTGG 1 cut(s) 1258
BseXI GCAGC 3 cut(s) 878, 886, 1060
BseYI CCCAGC 2 cut(s) 458, 614
BsgI GTGCAG 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 709
BshFI GGCC 2 cut(s) 706, 1208
BsiSI CCGG 2 cut(s) 188, 200
BslFI GGGAC 5 cut(s) 189, 346, 585, 695, 1135
BslI CCNNNNNNNGG 3 cut(s) 301, 650, 1110
BsmFI GGGAC 5 cut(s) 189, 346, 585, 695, 1135
BsnI GGCC 2 cut(s) 706, 1208
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 656
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 1 cut(s) 931
Bsp19I CCATGG 1 cut(s) 1209
BspACI CCGC 7 cut(s) 164, 341, 504, 584, 612, 707, 709
BspANI GGCC 2 cut(s) 706, 1208
BspCNI CTCAG 3 cut(s) 48, 292, 1119
BspFNI CGCG 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 204, 205
BspMI ACCTGC 1 cut(s) 1101
BspPI GGATC 1 cut(s) 926
BspT104I TTCGAA 1 cut(s) 278
BspTI CTTAAG 1 cut(s) 1001
BsrDI GCAATG 2 cut(s) 402, 1395
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 1 cut(s) 1258
BssECI CCNNGG 5 cut(s) 240, 707, 832, 833, 1209
BssMI GATC 1 cut(s) 931
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 240, 1209
Bst2UI CCWGG 1 cut(s) 834
Bst6I CTCTTC 5 cut(s) 34, 71, 103, 416, 814
BstACI GRCGYC 1 cut(s) 185
BstAFI CTTAAG 1 cut(s) 1001
BstAUI TGTACA 1 cut(s) 747
BstBAI YACGTR 1 cut(s) 1303
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 5 cut(s) 225, 378, 502, 612, 880
BstDEI CTNAG 4 cut(s) 35, 300, 941, 1106
BstDSI CCRYGG 2 cut(s) 707, 1209
BstF5I GGATG 5 cut(s) 42, 361, 976, 1321, 1377
BstFNI CGCG 1 cut(s) 709
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 1 cut(s) 934
BstMBI GATC 1 cut(s) 931
BstMWI GCNNNNNNNGC 2 cut(s) 718, 875
BstNI CCWGG 1 cut(s) 834
BstSCI CCNGG 2 cut(s) 199, 832
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 3 cut(s) 878, 886, 1060
BstXI CCANNNNNNTGG 2 cut(s) 1096, 1384
BsuRI GGCC 2 cut(s) 706, 1208
BtgI CCRYGG 2 cut(s) 707, 1209
BtsCI GGATG 5 cut(s) 42, 361, 976, 1321, 1377
BtsIMutI CAGTG 2 cut(s) 1251, 1334
BveI ACCTGC 1 cut(s) 1101
Cac8I GCNNGC 5 cut(s) 225, 378, 502, 612, 880
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 3 cut(s) 203, 704, 1086
Cfr42I CCGCGG 1 cut(s) 710
CseI GACGC 1 cut(s) 193
Csp6I GTAC 3 cut(s) 748, 1135, 1332
CviAII CATG 2 cut(s) 1064, 1210
CviQI GTAC 3 cut(s) 748, 1135, 1332
DdeI CTNAG 4 cut(s) 35, 300, 941, 1106
DpnI GATC 1 cut(s) 933
DpnII GATC 1 cut(s) 931
Eam1104I CTCTTC 5 cut(s) 34, 71, 103, 416, 814
EarI CTCTTC 5 cut(s) 34, 71, 103, 416, 814
EciI GGCGGA 1 cut(s) 519
Eco130I CCWWGG 2 cut(s) 240, 1209
Eco24I GRGCYC 1 cut(s) 656
Eco47I GGWCC 2 cut(s) 203, 1086
Eco57I CTGAAG 2 cut(s) 876, 1130
EcoO109I RGGNCCY 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 832
EcoT14I CCWWGG 2 cut(s) 240, 1209
EcoT38I GRGCYC 1 cut(s) 656
ErhI CCWWGG 2 cut(s) 240, 1209
FaeI CATG 2 cut(s) 1067, 1213
FaqI GGGAC 5 cut(s) 189, 346, 585, 695, 1135
FatI CATG 2 cut(s) 1063, 1209
FauI CCCGC 3 cut(s) 171, 334, 619
Fnu4HI GCNGC 5 cut(s) 707, 710, 867, 900, 1074
FokI GGATG 5 cut(s) 29, 368, 983, 1328, 1364
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 5 cut(s) 707, 710, 867, 900, 1074
FspBI CTAG 3 cut(s) 687, 783, 1164
GlaI GCGC 1 cut(s) 433
GluI GCNGC 5 cut(s) 707, 710, 867, 900, 1074
GsaI CCCAGC 2 cut(s) 462, 618
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 2 cut(s) 706, 1208
HapII CCGG 2 cut(s) 188, 200
HgaI GACGC 1 cut(s) 193
HhaI GCGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 2 cut(s) 1067, 1213
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 2 cut(s) 1142, 1275
HinfI GANTC 6 cut(s) 146, 573, 724, 977, 1100, 1339
HpaII CCGG 2 cut(s) 188, 200
HphI GGTGA 3 cut(s) 165, 458, 1230
Hpy166II GTNNAC 2 cut(s) 211, 1334
Hpy188I TCNGA 4 cut(s) 355, 634, 1026, 1297
Hpy188III TCNNGA 6 cut(s) 644, 659, 740, 1051, 1097, 1261
Hpy8I GTNNAC 2 cut(s) 211, 1334
Hpy99I CGWCG 1 cut(s) 187
HpyCH4IV ACGT 2 cut(s) 806, 1302
HpyCH4V TGCA 3 cut(s) 262, 395, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 718, 875
HpyF3I CTNAG 4 cut(s) 35, 300, 941, 1106
HpySE526I ACGT 2 cut(s) 806, 1302
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 2 cut(s) 1067, 1213
HspAI GCGC 1 cut(s) 432
KflI GGGWCCC 1 cut(s) 203
KspI CCGCGG 1 cut(s) 710
Kzo9I GATC 1 cut(s) 931
Lsp1109I GCAGC 3 cut(s) 878, 886, 1060
MaeI CTAG 3 cut(s) 687, 783, 1164
MaeII ACGT 2 cut(s) 806, 1302
MaeIII GTNAC 2 cut(s) 446, 1041
MalI GATC 1 cut(s) 933
MboI GATC 1 cut(s) 931
MboII GAAGA 9 cut(s) 21, 58, 90, 141, 433, 756, 831, 869, 1258
MhlI GDGCHC 1 cut(s) 656
MlyI GAGTC 4 cut(s) 567, 718, 971, 1094
MmeI TCCRAC 1 cut(s) 973
MseI TTAA 5 cut(s) 387, 453, 965, 1002, 1013
MslI CAYNNNNRTG 1 cut(s) 1214
MspA1I CMGCKG 3 cut(s) 614, 709, 854
MspCI CTTAAG 1 cut(s) 1001
MspI CCGG 2 cut(s) 188, 200
MspR9I CCNGG 2 cut(s) 201, 834
MvaI CCWGG 1 cut(s) 834
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 2 cut(s) 718, 875
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 1209
NdeII GATC 1 cut(s) 931
NlaIII CATG 2 cut(s) 1067, 1213
NlaIV GGNNCC 2 cut(s) 204, 205
NmeAIII GCCGAG 2 cut(s) 220, 555
NmuCI GTSAC 1 cut(s) 446
NspV TTCGAA 1 cut(s) 278
PasI CCCWGGG 1 cut(s) 833
PfeI GAWTC 2 cut(s) 146, 1339
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 5 cut(s) 708, 711, 868, 901, 1075
PleI GAGTC 4 cut(s) 567, 718, 971, 1094
PpsI GAGTC 4 cut(s) 567, 718, 971, 1094
Ppu21I YACGTR 1 cut(s) 1303
PpuMI RGGWCCY 1 cut(s) 203
PshBI ATTAAT 2 cut(s) 453, 1013
Psp1406I AACGTT 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 832
PspFI CCCAGC 2 cut(s) 458, 614
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 2 cut(s) 204, 205
PspPI GGNCC 3 cut(s) 203, 704, 1086
PspPPI RGGWCCY 1 cut(s) 203
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 3 cut(s) 749, 1136, 1333
RsaNI GTAC 3 cut(s) 748, 1135, 1332
RseI CAYNNNNRTG 1 cut(s) 1214
SacII CCGCGG 1 cut(s) 710
SaqAI TTAA 5 cut(s) 387, 453, 965, 1002, 1013
SatI GCNGC 5 cut(s) 707, 710, 867, 900, 1074
Sau3AI GATC 1 cut(s) 931
Sau96I GGNCC 3 cut(s) 203, 704, 1086
SchI GAGTC 4 cut(s) 567, 718, 971, 1094
ScrFI CCNGG 2 cut(s) 201, 834
SduI GDGCHC 1 cut(s) 656
Sfr303I CCGCGG 1 cut(s) 710
SfuI TTCGAA 1 cut(s) 278
SgrBI CCGCGG 1 cut(s) 710
SinI GGWCC 2 cut(s) 203, 1086
SmiMI CAYNNNNRTG 1 cut(s) 1214
SmlI CTYRAG 3 cut(s) 726, 1001, 1125
SmoI CTYRAG 3 cut(s) 726, 1001, 1125
SsiI CCGC 7 cut(s) 164, 341, 504, 584, 612, 707, 709
SspMI CTAG 3 cut(s) 687, 783, 1164
StyD4I CCNGG 2 cut(s) 199, 832
StyI CCWWGG 2 cut(s) 240, 1209
TaiI ACGT 2 cut(s) 809, 1305
TaqI TCGA 3 cut(s) 278, 666, 1323
TaqII GACCGA 1 cut(s) 802
TatI WGTACW 1 cut(s) 747
TauI GCSGC 2 cut(s) 709, 712
TfiI GAWTC 2 cut(s) 146, 1339
Tru1I TTAA 5 cut(s) 387, 453, 965, 1002, 1013
Tru9I TTAA 5 cut(s) 387, 453, 965, 1002, 1013
TscAI CASTG 2 cut(s) 1258, 1341
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 3 cut(s) 866, 899, 1073
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 6 cut(s) 9, 281, 1001, 1052, 1270, 1332
TspRI CASTG 2 cut(s) 1258, 1341
Tth111I GACNNNGTC 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 1001
VpaK11BI GGWCC 2 cut(s) 203, 1086
VspI ATTAAT 2 cut(s) 453, 1013
XapI RAATTY 5 cut(s) 113, 169, 274, 508, 691
XspI CTAG 3 cut(s) 687, 783, 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.