Rh5AG465500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000939
Physical Location & Seq
Forward (+)
79777926 .. 79784588
6663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG465500.1

Sequence Viewer

Length: 1224 bp
ATGCCGAATCCGAAACCACGACGTCCCCAGTCGACCCACCAATTCCTGGGAATGTCGAATTGCCTGCTGCTGTTAAGACTGCAATGGAGAGAGCTAGAGAGTAAGCAGAAGAAGACAGGGACTAGCAAAACTTCAGGTGTTGAAGCAAATGAGAAAAACAAGGGAAAATTGTCAGTGTCGAGCATGGATTTTGTGGGACTTGGGTTTGCTGATAAGAAGGAGGGTAGAGCACTGCCAGCTGGGTTGGTTCCAATTGCAGACGATTTTGCTCAGGGCAACTTGCCTGAGGTTGAGATTATTGTTGGGGACTCCAGTAATTTCGGTGCAGCCACACCCAAGCCTCAAGGAGATGACTCCCTGGATCTTTACAAGCCAAAGGTTTCTTCATGGGGAGTCTTTCCCAGACCCAATGACATTTCAAAAACGTTTGGTGGTGGGCGAGTTATACACCCCGGGGAAGTGCTTGAAACGCAGGAAGAAAAAGCTGCTAAGGAGGAACGAACAAGACAATTAGTTGCTGCTTACAACACTAAAATGGGCTTGAACATTGATCCCAAGCTCAGATTGGACTGTGAGAAGACATTAAAGGATGGTGACTCATTGATGAACGTCAGAAAGCTGAAGGAAGCATTACCCTACTATGAGAAAGTTATGGATAAGTTACCATTCAAGATTATGACTCCGAAAGCTCGTGCAGACATACACATCAATCTTCCAGCACTTAAGAAGTTGGACTCGATACTTATTGAAACACTTGATTCAATGGTGGATACTGAGTTTGGGTATGTAGAAGGTTCTGGATTGGCTTTTTTGGCTGAGGTGTCTTCGGTTGGACATTTGGACAGAAATTGCAAACCACTCGCTTCAACTTTAAAGATTGGAGCTACTTTCCCTCTCAACTCAAACCCTAGCCCCTCCTCCTCTTGTCTCACAGCTCGCCTGAGGCTACTGAATGTGATCATGGCCGACGTCGCCACCCTGTCGTGTGCTCGTTGCAGCAAACCCGCCAATCTTCAGTGTCCCAAGTGTGTGGAATTGAAGCTTCCTCGTGAAGATGCCGCTTTCTGGACATTGAGACCATATCCTATATCTAGTAAGCGTGTGGTACCTTCTCATATTAATCTACCTGATTGGGCAGATGATGGAATCCCAAAAGAGGAACCCAGTAGTGACCTGCAGCGTGTTGTTGAGGTGTTCTTTTTTCTTATTTTGGAAAATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

44.91

Weight (kDa)

8.48

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 207 - 266 4.8e-16 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 25, 972
Acc36I ACCTGC 1 cut(s) 1182
Acc65I GGTACC 1 cut(s) 1105
AccB1I GGYRCC 1 cut(s) 1105
AccB7I CCANNNNNTGG 1 cut(s) 46
AccI GTMKAC 1 cut(s) 32
AciI CCGC 2 cut(s) 1005, 1059
AclI AACGTT 1 cut(s) 425
AclWI GGATC 2 cut(s) 369, 545
AcoI YGGCCR 1 cut(s) 963
AcuI CTGAAG 3 cut(s) 117, 641, 998
AcyI GRCGYC 2 cut(s) 22, 969
AfaI GTAC 1 cut(s) 1107
AfiI CCNNNNNNNGG 3 cut(s) 46, 1065, 1156
AflII CTTAAG 1 cut(s) 722
AgsI TTSAA 9 cut(s) 143, 420, 467, 544, 670, 749, 762, 867, 1039
AjnI CCWGG 2 cut(s) 45, 357
AjuI GAANNNNNNNTTGG 2 cut(s) 367, 399
AluBI AGCT 9 cut(s) 94, 239, 485, 559, 619, 689, 884, 935, 1042
AluI AGCT 9 cut(s) 94, 239, 485, 559, 619, 689, 884, 935, 1042
Alw21I GWGCWC 2 cut(s) 232, 991
Alw26I GTCTC 2 cut(s) 932, 1069
AlwI GGATC 2 cut(s) 369, 545
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 963
ApeKI GCWGC 6 cut(s) 67, 326, 485, 518, 996, 1177
AseI ATTAAT 1 cut(s) 1119
Asp718I GGTACC 1 cut(s) 1105
AsuC2I CCSGG 2 cut(s) 453, 454
AsuHPI GGTGA 1 cut(s) 605
AvaI CYCGRG 1 cut(s) 452
AxyI CCTNAGG 2 cut(s) 285, 941
BanI GGYRCC 1 cut(s) 1105
BauI CACGAG 2 cut(s) 690, 1047
BbsI GAAGAC 3 cut(s) 119, 584, 816
Bbv12I GWGCWC 2 cut(s) 232, 991
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 6 cut(s) 54, 338, 472, 505, 1008, 1189
BccI CCATC 2 cut(s) 584, 1136
BcgI CGANNNNNNTGC 4 cut(s) 46, 80, 841, 875
BciT130I CCWGG 2 cut(s) 47, 359
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 957
BcnI CCSGG 2 cut(s) 453, 454
BcoDI GTCTC 2 cut(s) 932, 1069
BfaI CTAG 4 cut(s) 95, 123, 909, 1092
BfmI CTRYAG 1 cut(s) 1175
BfrI CTTAAG 1 cut(s) 722
BfuAI ACCTGC 1 cut(s) 1182
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 7 cut(s) 68, 327, 486, 519, 997, 1059, 1178
BlsI GCNGC 7 cut(s) 69, 328, 487, 520, 998, 1060, 1179
Bme1390I CCNGG 4 cut(s) 47, 359, 453, 454
BmeT110I CYCGRG 1 cut(s) 452
BmiI GGNNCC 3 cut(s) 249, 1107, 1161
BmrFI CCNGG 4 cut(s) 47, 359, 453, 454
BmrI ACTGGG 2 cut(s) 22, 1158
BmsI GCATC 1 cut(s) 1045
BmuI ACTGGG 2 cut(s) 22, 1158
BpiI GAAGAC 3 cut(s) 119, 584, 816
BpmI CTGGAG 1 cut(s) 295
Bpu10I CCTNAGC 3 cut(s) 270, 489, 816
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 2 cut(s) 453, 454
BsaHI GRCGYC 2 cut(s) 22, 969
BsaI GGTCTC 1 cut(s) 1069
BsaJI CCNNGG 5 cut(s) 46, 357, 451, 452, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 1065, 1156
Bse1I ACTGG 3 cut(s) 28, 312, 1164
Bse21I CCTNAGG 2 cut(s) 285, 941
Bse3DI GCAATG 1 cut(s) 89
BseBI CCWGG 2 cut(s) 47, 359
BseDI CCNNGG 5 cut(s) 46, 357, 451, 452, 453
BseGI GGATG 1 cut(s) 595
BseLI CCNNNNNNNGG 3 cut(s) 46, 1065, 1156
BseMI GCAATG 1 cut(s) 89
BseMII CTCAG 6 cut(s) 276, 284, 574, 765, 807, 932
BseNI ACTGG 3 cut(s) 28, 312, 1164
BseRI GAGGAG 2 cut(s) 907, 910
BseXI GCAGC 6 cut(s) 54, 338, 472, 505, 1008, 1189
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 345, 714
BshFI GGCC 1 cut(s) 965
BshNI GGYRCC 1 cut(s) 1105
BsiHKAI GWGCWC 2 cut(s) 232, 991
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 1 cut(s) 453
BslFI GGGAC 5 cut(s) 9, 133, 210, 320, 1005
BslI CCNNNNNNNGG 3 cut(s) 46, 1065, 1156
BsmAI GTCTC 2 cut(s) 932, 1069
BsmFI GGGAC 5 cut(s) 9, 133, 210, 320, 1005
BsnI GGCC 1 cut(s) 965
Bso31I GGTCTC 1 cut(s) 1069
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 232, 991
Bsp143I GATC 3 cut(s) 361, 550, 957
BspACI CCGC 2 cut(s) 1005, 1059
BspANI GGCC 1 cut(s) 965
BspCNI CTCAG 6 cut(s) 277, 283, 573, 766, 808, 933
BspLI GGNNCC 3 cut(s) 249, 1107, 1161
BspMAI CTGCAG 1 cut(s) 1179
BspMI ACCTGC 1 cut(s) 1182
BspPI GGATC 2 cut(s) 369, 545
BspT107I GGYRCC 1 cut(s) 1105
BspTI CTTAAG 1 cut(s) 722
BspTNI GGTCTC 1 cut(s) 1069
BsrDI GCAATG 1 cut(s) 89
BsrI ACTGG 3 cut(s) 28, 312, 1164
BssECI CCNNGG 5 cut(s) 46, 357, 451, 452, 453
BssMI GATC 3 cut(s) 361, 550, 957
BssNI GRCGYC 2 cut(s) 22, 969
BssSI CACGAG 2 cut(s) 690, 1047
Bst2BI CACGAG 2 cut(s) 690, 1047
Bst2UI CCWGG 2 cut(s) 47, 359
Bst4CI ACNGT 1 cut(s) 572
BstACI GRCGYC 2 cut(s) 22, 969
BstAFI CTTAAG 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 65, 237, 937
BstDEI CTNAG 7 cut(s) 270, 285, 489, 560, 774, 816, 941
BstF5I GGATG 1 cut(s) 595
BstKTI GATC 3 cut(s) 364, 553, 960
BstMAI GTCTC 2 cut(s) 932, 1069
BstMBI GATC 3 cut(s) 361, 550, 957
BstMWI GCNNNNNNNGC 4 cut(s) 236, 469, 812, 971
BstNI CCWGG 2 cut(s) 47, 359
BstSCI CCNGG 4 cut(s) 45, 357, 451, 452
BstSFI CTRYAG 1 cut(s) 1175
BstV1I GCAGC 6 cut(s) 54, 338, 472, 505, 1008, 1189
BstV2I GAAGAC 3 cut(s) 119, 584, 816
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 1 cut(s) 361
Bsu36I CCTNAGG 2 cut(s) 285, 941
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 1 cut(s) 965
BtsCI GGATG 1 cut(s) 595
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 3 cut(s) 180, 230, 1022
BveI ACCTGC 1 cut(s) 1182
Cac8I GCNNGC 3 cut(s) 65, 237, 937
Cfr9I CCCGGG 1 cut(s) 452
Csp6I GTAC 1 cut(s) 1106
CviAII CATG 3 cut(s) 184, 387, 961
CviQI GTAC 1 cut(s) 1106
DdeI CTNAG 7 cut(s) 270, 285, 489, 560, 774, 816, 941
DpnI GATC 3 cut(s) 363, 552, 959
DpnII GATC 3 cut(s) 361, 550, 957
DraI TTTAAA 1 cut(s) 873
EaeI YGGCCR 1 cut(s) 963
Eco31I GGTCTC 1 cut(s) 1069
Eco57I CTGAAG 3 cut(s) 117, 641, 998
Eco81I CCTNAGG 2 cut(s) 285, 941
Eco88I CYCGRG 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 45, 357
FaeI CATG 3 cut(s) 187, 390, 964
FaqI GGGAC 5 cut(s) 9, 133, 210, 320, 1005
FatI CATG 3 cut(s) 183, 386, 960
FauI CCCGC 1 cut(s) 1012
FbaI TGATCA 1 cut(s) 957
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 7 cut(s) 68, 327, 486, 519, 997, 1059, 1178
FokI GGATG 1 cut(s) 602
Fsp4HI GCNGC 7 cut(s) 68, 327, 486, 519, 997, 1059, 1178
FspBI CTAG 4 cut(s) 95, 123, 909, 1092
GluI GCNGC 7 cut(s) 68, 327, 486, 519, 997, 1059, 1178
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 1 cut(s) 965
HapII CCGG 1 cut(s) 453
Hin1I GRCGYC 2 cut(s) 22, 969
Hin1II CATG 3 cut(s) 187, 390, 964
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 9 cut(s) 7, 308, 353, 393, 596, 679, 734, 758, 1146
HpaII CCGG 1 cut(s) 453
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 4 cut(s) 12, 563, 614, 684
Hpy188III TCNNGA 4 cut(s) 670, 798, 1049, 1066
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 3 cut(s) 24, 971, 974
HpyAV CCTTC 4 cut(s) 211, 616, 785, 1119
HpyCH4III ACNGT 1 cut(s) 572
HpyCH4IV ACGT 4 cut(s) 22, 425, 609, 969
HpyCH4V TGCA 7 cut(s) 82, 257, 326, 695, 852, 996, 1177
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 469, 812, 971
HpyF3I CTNAG 7 cut(s) 270, 285, 489, 560, 774, 816, 941
HpySE526I ACGT 4 cut(s) 22, 425, 609, 969
Hsp92I GRCGYC 2 cut(s) 22, 969
Hsp92II CATG 3 cut(s) 187, 390, 964
KpnI GGTACC 1 cut(s) 1109
Ksp22I TGATCA 1 cut(s) 957
Kzo9I GATC 3 cut(s) 361, 550, 957
LmnI GCTCC 1 cut(s) 881
Lsp1109I GCAGC 6 cut(s) 54, 338, 472, 505, 1008, 1189
LweI GCATC 1 cut(s) 1045
MaeI CTAG 4 cut(s) 95, 123, 909, 1092
MaeII ACGT 4 cut(s) 22, 425, 609, 969
MaeIII GTNAC 3 cut(s) 593, 660, 1169
MalI GATC 3 cut(s) 363, 552, 959
MboI GATC 3 cut(s) 361, 550, 957
MboII GAAGA 9 cut(s) 121, 124, 375, 488, 589, 704, 816, 1004, 1064
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 232, 991
MluCI AATT 8 cut(s) 41, 58, 167, 252, 316, 509, 847, 1034
MlyI GAGTC 6 cut(s) 302, 347, 402, 590, 673, 728
MmeI TCCRAC 2 cut(s) 711, 811
MseI TTAA 6 cut(s) 74, 584, 723, 872, 1119, 1222
MslI CAYNNNNRTG 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 239
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 4 cut(s) 47, 359, 453, 454
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 2 cut(s) 47, 359
MwoI GCNNNNNNNGC 4 cut(s) 236, 469, 812, 971
NciI CCSGG 2 cut(s) 453, 454
NdeII GATC 3 cut(s) 361, 550, 957
NlaIII CATG 3 cut(s) 187, 390, 964
NlaIV GGNNCC 3 cut(s) 249, 1107, 1161
NmuCI GTSAC 2 cut(s) 593, 1169
PfeI GAWTC 3 cut(s) 7, 758, 1146
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 7 cut(s) 69, 328, 487, 520, 998, 1060, 1179
PleI GAGTC 6 cut(s) 302, 347, 401, 590, 673, 728
PpsI GAGTC 6 cut(s) 302, 347, 401, 590, 673, 728
PshBI ATTAAT 1 cut(s) 1119
Psp1406I AACGTT 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 45, 357
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 2 cut(s) 45, 357
PspN4I GGNNCC 3 cut(s) 249, 1107, 1161
PstI CTGCAG 1 cut(s) 1179
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 1107
RsaNI GTAC 1 cut(s) 1106
RseI CAYNNNNRTG 1 cut(s) 533
SalI GTCGAC 1 cut(s) 31
SaqAI TTAA 6 cut(s) 74, 584, 723, 872, 1119, 1222
SatI GCNGC 7 cut(s) 68, 327, 486, 519, 997, 1059, 1178
Sau3AI GATC 3 cut(s) 361, 550, 957
SchI GAGTC 6 cut(s) 302, 347, 402, 590, 673, 728
ScrFI CCNGG 4 cut(s) 47, 359, 453, 454
SduI GDGCHC 2 cut(s) 232, 991
SfaNI GCATC 1 cut(s) 1045
SfcI CTRYAG 1 cut(s) 1175
SmaI CCCGGG 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 2 cut(s) 342, 722
SmoI CTYRAG 2 cut(s) 342, 722
Sse9I AATT 8 cut(s) 41, 58, 167, 252, 316, 509, 847, 1034
SsiI CCGC 2 cut(s) 1005, 1059
SspMI CTAG 4 cut(s) 95, 123, 909, 1092
StyD4I CCNGG 4 cut(s) 45, 357, 451, 452
TaaI ACNGT 1 cut(s) 572
TaiI ACGT 4 cut(s) 25, 428, 612, 972
TaqI TCGA 4 cut(s) 32, 56, 179, 737
TasI AATT 8 cut(s) 41, 58, 167, 252, 316, 509, 847, 1034
TauI GCSGC 1 cut(s) 1061
TfiI GAWTC 3 cut(s) 7, 758, 1146
Tru1I TTAA 6 cut(s) 74, 584, 723, 872, 1119, 1222
Tru9I TTAA 6 cut(s) 74, 584, 723, 872, 1119, 1222
TscAI CASTG 3 cut(s) 180, 237, 1022
TseFI GTSAC 2 cut(s) 593, 1169
TseI GCWGC 6 cut(s) 67, 326, 485, 518, 996, 1177
Tsp45I GTSAC 2 cut(s) 593, 1169
TspDTI ATGAA 2 cut(s) 375, 620
TspMI CCCGGG 1 cut(s) 452
TspRI CASTG 3 cut(s) 180, 237, 1022
Van91I CCANNNNNTGG 1 cut(s) 46
Vha464I CTTAAG 1 cut(s) 722
VspI ATTAAT 1 cut(s) 1119
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmaI CCCGGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 32
XspI CTAG 4 cut(s) 95, 123, 909, 1092
ZraI GACGTC 2 cut(s) 23, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.