MD02G1293900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000939
Physical Location & Seq
Forward (+)
34910138 .. 34914967
4830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1293900.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGGCTTCCCTTCAACCTTCATTCTTCTCTTCCCTCAGCACCATCCCTTCCACCAAACCCACTCTCTTCCTCTCCATAAACCCTAACAAACCCCACCCTTCAAAACCATTCAAACTCTCCTTCTCCCTGAACCCACCCAACGCTGAATCTTCACAACCCACCTCGCCAAATTCACCCGAAACGACGCCTGAAGCCGAGCCGGGTCCCGTCGACCCTGTCAAGCTCGCATTGGCGAACGCCAAGGCGTACAAGAAGTCGGTGCAGATGAACAAGAATTTGAAAATTGAGAAAAACCCTGCTGAGGATAGTGGTGGAATTGCTGGAAATGGTGGGAGTTCGGGTTCGGATGGTGGGGCGAAAGAACTGCCTGCTTCGGTTAAGATTGCAATGGAGAAAGCTAAAGAGTATAAGAAGAGTAAAGGCACTGTGGGTGGTGGTGACATCAATGCTGGGGAGAACAAAGGCACAATTTCAGGTTTGAAGACAAGTGCTGGTGGAAATTCGGGGAAAGAGGTAGTGGAAAAGAAGGCGAAATTGTCAGTTTCAAGCATTGATTTTGTGGGACTCGGCTTTGCGGATAAGAAGGAGGGCAGAGGACTGCCCGCTGGGTTAGTTCCAATATCGGACTATATTCCAGAAGGGGGCTCTCCTGATGTCGAGTTTATAGTGGGGGACACTAGAAATTTCGATGCAGGGCCACGGCAGACAGAGCAGACTCAAGGAGATAATCCAGACCTGTACAAGCCAAAAGTTTCTTCGTGGGGAGTATTTCCTAGACCGAACGACATTTCCAAAACGTTTGGAGGTGGAAGAGTTATACGCCCAGGGCAAGTGCTGGAAACAGCTGAAGAAAAAGCAGCTAAAGAAGCACGCACAAGACAATTAGTAGCTGCTTACAAGAGTACAATGGGGTTGAACATTGATCCAAAGCTAAGAGCTGAATGCGAGAAGGCGTTAAAGGATGGCGACTCATTGATGAATGTTGGAGAGCTTAAGGAAGCATTAATCTATTATCAGAAGGTTATGGATAAGTTACCATTTAAGAGTGAGCTTCATGGGTTGGCTGCTTTACAATGGTCCATTTGTCTAGACTCCCTCAGCAGGTCAAAGGAAGCTCAAGTTATGTACGAGAAGCTTCAGTCCCACCCAACTCCTAGAGTAAGTAAGAAGGCGAGGCAATTTGTGTTCAGCTTTCAGGCCATGGAAATGTTGAGGCTTACGAAGAACTCACCTTTGAAGAACACTGGCTTCCAGAATTATTTTGAAGCTTTCATAGAAAACAAATCGGACTACGTGCTAAAAGAGGATGAAGTAGAAGTAGGTACACTGAATCAAACTCTACCATATATCATTTTTCTTGTTTCCCCCATCCTTTTGGTGCTACTCATTGCTATACAAAAGAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

470

Amino Acids

50.73

Weight (kDa)

9.12

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1092
AccI GTMKAC 1 cut(s) 210
AciI CCGC 2 cut(s) 575, 603
AclI AACGTT 1 cut(s) 797
AclWI GGATC 1 cut(s) 917
AcsI RAATTY 4 cut(s) 169, 274, 499, 682
AcuI CTGAAG 3 cut(s) 210, 867, 1121
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 5 cut(s) 248, 740, 904, 1127, 1324
AfiI CCNNNNNNNGG 3 cut(s) 301, 641, 1101
AflII CTTAAG 1 cut(s) 992
AgsI TTSAA 9 cut(s) 14, 102, 112, 280, 481, 546, 916, 1237, 1265
AjnI CCWGG 1 cut(s) 823
AjuI GAANNNNNNNTTGG 2 cut(s) 739, 771
AloI GAACNNNNNNTCC 4 cut(s) 325, 357, 773, 805
AlwI GGATC 1 cut(s) 917
AoxI GGCC 2 cut(s) 695, 1197
ApeKI GCWGC 3 cut(s) 857, 890, 1064
ApoI RAATTY 4 cut(s) 169, 274, 499, 682
AseI ATTAAT 1 cut(s) 1004
AspS9I GGNCC 3 cut(s) 203, 695, 1077
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 3 cut(s) 165, 449, 1221
AvaII GGWCC 2 cut(s) 203, 1077
BanII GRGCYC 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 488
BbvCI CCTCAGC 3 cut(s) 35, 300, 1097
BbvI GCAGC 3 cut(s) 869, 877, 1051
BccI CCATC 4 cut(s) 50, 341, 956, 1376
BceAI ACGGC 1 cut(s) 716
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 1 cut(s) 825
BcnI CCSGG 1 cut(s) 201
BfaI CTAG 4 cut(s) 678, 774, 1088, 1155
BfrI CTTAAG 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 1092
BisI GCNGC 3 cut(s) 858, 891, 1065
BlsI GCNGC 3 cut(s) 859, 892, 1066
Bme1390I CCNGG 2 cut(s) 201, 825
Bme18I GGWCC 2 cut(s) 203, 1077
BmgT120I GGNCC 3 cut(s) 203, 695, 1077
BmiI GGNNCC 2 cut(s) 204, 205
BmrFI CCNGG 2 cut(s) 201, 825
BmsI GCATC 1 cut(s) 679
BpiI GAAGAC 1 cut(s) 488
Bpu10I CCTNAGC 3 cut(s) 35, 300, 1097
BpuEI CTTGAG 2 cut(s) 702, 1101
BpuMI CCSGG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 1294
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 5 cut(s) 240, 698, 823, 824, 1200
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 641, 1101
Bse1I ACTGG 1 cut(s) 1249
Bse3DI GCAATG 2 cut(s) 393, 1386
BseBI CCWGG 1 cut(s) 825
BseDI CCNNGG 5 cut(s) 240, 698, 823, 824, 1200
BseGI GGATG 5 cut(s) 42, 352, 967, 1312, 1368
BseLI CCNNNNNNNGG 3 cut(s) 301, 641, 1101
BseMI GCAATG 2 cut(s) 393, 1386
BseMII CTCAG 3 cut(s) 49, 291, 1111
BseNI ACTGG 1 cut(s) 1249
BseXI GCAGC 3 cut(s) 869, 877, 1051
BseYI CCCAGC 2 cut(s) 449, 605
BsgI GTGCAG 1 cut(s) 281
BshFI GGCC 2 cut(s) 697, 1199
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 4 cut(s) 189, 576, 686, 1126
BslI CCNNNNNNNGG 3 cut(s) 301, 641, 1101
BsmFI GGGAC 4 cut(s) 189, 576, 686, 1126
BsmI GAATGC 1 cut(s) 947
BsnI GGCC 2 cut(s) 697, 1199
Bsp1286I GDGCHC 1 cut(s) 647
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 1 cut(s) 922
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 2 cut(s) 575, 603
BspANI GGCC 2 cut(s) 697, 1199
BspCNI CTCAG 3 cut(s) 48, 292, 1110
BspLI GGNNCC 2 cut(s) 204, 205
BspMI ACCTGC 1 cut(s) 1092
BspPI GGATC 1 cut(s) 917
BspTI CTTAAG 1 cut(s) 992
BsrDI GCAATG 2 cut(s) 393, 1386
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 1 cut(s) 1249
BssECI CCNNGG 5 cut(s) 240, 698, 823, 824, 1200
BssMI GATC 1 cut(s) 922
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 240, 1200
Bst2UI CCWGG 1 cut(s) 825
Bst4CI ACNGT 1 cut(s) 427
Bst6I CTCTTC 4 cut(s) 34, 71, 407, 805
BstACI GRCGYC 1 cut(s) 185
BstAFI CTTAAG 1 cut(s) 992
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 1 cut(s) 1294
BstC8I GCNNGC 4 cut(s) 225, 369, 603, 871
BstDEI CTNAG 4 cut(s) 35, 300, 932, 1097
BstDSI CCRYGG 2 cut(s) 698, 1200
BstF5I GGATG 5 cut(s) 42, 352, 967, 1312, 1368
BstKTI GATC 1 cut(s) 925
BstMBI GATC 1 cut(s) 922
BstMWI GCNNNNNNNGC 2 cut(s) 709, 866
BstNI CCWGG 1 cut(s) 825
BstSCI CCNGG 2 cut(s) 199, 823
BstV1I GCAGC 3 cut(s) 869, 877, 1051
BstV2I GAAGAC 1 cut(s) 488
BstXI CCANNNNNNTGG 1 cut(s) 1375
BsuRI GGCC 2 cut(s) 697, 1199
BtgI CCRYGG 2 cut(s) 698, 1200
BtsCI GGATG 5 cut(s) 42, 352, 967, 1312, 1368
BtsIMutI CAGTG 3 cut(s) 423, 1242, 1325
BveI ACCTGC 1 cut(s) 1092
Cac8I GCNNGC 4 cut(s) 225, 369, 603, 871
Cfr13I GGNCC 3 cut(s) 203, 695, 1077
CseI GACGC 1 cut(s) 193
Csp6I GTAC 5 cut(s) 247, 739, 903, 1126, 1323
CviAII CATG 2 cut(s) 1055, 1201
CviQI GTAC 5 cut(s) 247, 739, 903, 1126, 1323
DdeI CTNAG 4 cut(s) 35, 300, 932, 1097
DpnI GATC 1 cut(s) 924
DpnII GATC 1 cut(s) 922
Eam1104I CTCTTC 4 cut(s) 34, 71, 407, 805
EarI CTCTTC 4 cut(s) 34, 71, 407, 805
Eco130I CCWWGG 2 cut(s) 240, 1200
Eco24I GRGCYC 1 cut(s) 647
Eco47I GGWCC 2 cut(s) 203, 1077
Eco57I CTGAAG 3 cut(s) 210, 867, 1121
EcoO109I RGGNCCY 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 823
EcoT14I CCWWGG 2 cut(s) 240, 1200
EcoT38I GRGCYC 1 cut(s) 647
ErhI CCWWGG 2 cut(s) 240, 1200
FaeI CATG 2 cut(s) 1058, 1204
FaqI GGGAC 4 cut(s) 189, 576, 686, 1126
FatI CATG 2 cut(s) 1054, 1200
FauI CCCGC 1 cut(s) 610
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 3 cut(s) 858, 891, 1065
FokI GGATG 5 cut(s) 29, 359, 974, 1319, 1355
FriOI GRGCYC 1 cut(s) 647
Fsp4HI GCNGC 3 cut(s) 858, 891, 1065
FspBI CTAG 4 cut(s) 678, 774, 1088, 1155
GluI GCNGC 3 cut(s) 858, 891, 1065
GsaI CCCAGC 2 cut(s) 453, 609
HaeIII GGCC 2 cut(s) 697, 1199
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 2 cut(s) 1058, 1204
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 2 cut(s) 1133, 1266
HinfI GANTC 6 cut(s) 146, 564, 715, 968, 1091, 1330
HpaII CCGG 1 cut(s) 200
HphI GGTGA 3 cut(s) 165, 449, 1221
Hpy166II GTNNAC 2 cut(s) 211, 1325
Hpy188I TCNGA 4 cut(s) 346, 625, 1017, 1288
Hpy188III TCNNGA 5 cut(s) 635, 650, 731, 1088, 1252
Hpy8I GTNNAC 2 cut(s) 211, 1325
Hpy99I CGWCG 2 cut(s) 187, 212
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 2 cut(s) 797, 1293
HpyCH4V TGCA 3 cut(s) 262, 386, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 709, 866
HpyF3I CTNAG 4 cut(s) 35, 300, 932, 1097
HpySE526I ACGT 2 cut(s) 797, 1293
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 2 cut(s) 1058, 1204
KflI GGGWCCC 1 cut(s) 203
Kzo9I GATC 1 cut(s) 922
Lsp1109I GCAGC 3 cut(s) 869, 877, 1051
LweI GCATC 1 cut(s) 679
MaeI CTAG 4 cut(s) 678, 774, 1088, 1155
MaeII ACGT 2 cut(s) 797, 1293
MaeIII GTNAC 2 cut(s) 437, 1032
MalI GATC 1 cut(s) 924
MboI GATC 1 cut(s) 922
MhlI GDGCHC 1 cut(s) 647
MlyI GAGTC 4 cut(s) 558, 709, 962, 1085
MmeI TCCRAC 1 cut(s) 964
MseI TTAA 5 cut(s) 378, 956, 993, 1004, 1041
MslI CAYNNNNRTG 1 cut(s) 1205
MspA1I CMGCKG 2 cut(s) 605, 845
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 201, 825
Mva1269I GAATGC 1 cut(s) 947
MvaI CCWGG 1 cut(s) 825
MwoI GCNNNNNNNGC 2 cut(s) 709, 866
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 1200
NdeII GATC 1 cut(s) 922
NlaIII CATG 2 cut(s) 1058, 1204
NlaIV GGNNCC 2 cut(s) 204, 205
NmeAIII GCCGAG 2 cut(s) 220, 546
NmuCI GTSAC 1 cut(s) 437
PasI CCCWGGG 1 cut(s) 824
PctI GAATGC 1 cut(s) 947
PfeI GAWTC 2 cut(s) 146, 1330
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 3 cut(s) 859, 892, 1066
PleI GAGTC 4 cut(s) 558, 709, 962, 1085
PpsI GAGTC 4 cut(s) 558, 709, 962, 1085
Ppu21I YACGTR 1 cut(s) 1294
PpuMI RGGWCCY 1 cut(s) 203
PshBI ATTAAT 1 cut(s) 1004
Psp1406I AACGTT 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 823
PspFI CCCAGC 2 cut(s) 449, 605
PspGI CCWGG 1 cut(s) 823
PspN4I GGNNCC 2 cut(s) 204, 205
PspPI GGNCC 3 cut(s) 203, 695, 1077
PspPPI RGGWCCY 1 cut(s) 203
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 5 cut(s) 248, 740, 904, 1127, 1324
RsaNI GTAC 5 cut(s) 247, 739, 903, 1126, 1323
RseI CAYNNNNRTG 1 cut(s) 1205
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 5 cut(s) 378, 956, 993, 1004, 1041
SatI GCNGC 3 cut(s) 858, 891, 1065
Sau3AI GATC 1 cut(s) 922
Sau96I GGNCC 3 cut(s) 203, 695, 1077
SchI GAGTC 4 cut(s) 558, 709, 962, 1085
ScrFI CCNGG 2 cut(s) 201, 825
SduI GDGCHC 1 cut(s) 647
SfaNI GCATC 1 cut(s) 679
SinI GGWCC 2 cut(s) 203, 1077
SmiMI CAYNNNNRTG 1 cut(s) 1205
SmlI CTYRAG 3 cut(s) 717, 992, 1116
SmoI CTYRAG 3 cut(s) 717, 992, 1116
SsiI CCGC 2 cut(s) 575, 603
SspMI CTAG 4 cut(s) 678, 774, 1088, 1155
StyD4I CCNGG 2 cut(s) 199, 823
StyI CCWWGG 2 cut(s) 240, 1200
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 2 cut(s) 800, 1296
TaqI TCGA 3 cut(s) 210, 657, 687
TaqII GACCGA 1 cut(s) 793
TatI WGTACW 2 cut(s) 738, 902
TfiI GAWTC 2 cut(s) 146, 1330
Tru1I TTAA 5 cut(s) 378, 956, 993, 1004, 1041
Tru9I TTAA 5 cut(s) 378, 956, 993, 1004, 1041
TscAI CASTG 3 cut(s) 430, 1249, 1332
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 3 cut(s) 857, 890, 1064
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 6 cut(s) 9, 281, 992, 1043, 1261, 1323
TspRI CASTG 3 cut(s) 430, 1249, 1332
Tth111I GACNNNGTC 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 992
VpaK11BI GGWCC 2 cut(s) 203, 1077
VspI ATTAAT 1 cut(s) 1004
XapI RAATTY 4 cut(s) 169, 274, 499, 682
XbaI TCTAGA 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 4 cut(s) 678, 774, 1088, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.