Rh2CG244400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000939
Physical Location & Seq
Forward (+)
25626911 .. 25629704
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG244400.1

Sequence Viewer

Length: 756 bp
ATGGAGAGAGCTAGAGAGTATAAGCAGAAGAAGACAGGGACTAGCAAAACTTCAGGTGTTGAAGCAAATGAGAAAAACAAGGGAAAATTGTCAGTGTCGAGCATGGATTTTGTGGGTCTTGGGTTTGCTGATAAGAAGGAGGGTAGAGCACTACCAGCTGGGTTGGTTCCAATCGCAGACGATTTTGCTCAGGGCAACTTGCCTGAGGTTGAGATTATTGTTGGGGACTCCAGTAATTTCGGTGCAGCCACACCCAAGCCTCAAGGAGATGACCCCCTGGATCTTTACAAGCCAAAGTTTGGTGGTGGGCGAGTTATACACCCCGGGGAAGTGCTTGAAACACAGGAAGAAAAAGCTGCTAAGGAGGAACGAACAAGACAATTAGTTGCTACTTACAACACTAAAATGGGCTTGAACATTGATCCCAAGCTCAGATTGGACTGTGAGAAGGATTGCTTCGCTTCTTTTTTCTTTTTGAATTTAAAAGCTTTTCTTCCCTGGCCTCCCTTGATTTTTATTACATTAAAGGATGGCGACTCATTGATGAACGTCGGAAAGCTGAAGGAAGCATTACCCTACTATGAGAAAGTTATGGATAAGTTACCATTCAAGATTATGACTCCGAAAGCTCGTGCAGACATACACATCAATCTTCCAGCACTTAAGAAGTTGGACTCGATGCTTATTGAAACACTTGATTCAATGGTGGATACTGAGTTTGGGTATGTAGAAGGTGGTAGTAGGGCAGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.71

Weight (kDa)

5.89

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 188 - 249 7e-18 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 299
AclWI GGATC 2 cut(s) 288, 416
AcsI RAATTY 1 cut(s) 478
AcuI CTGAAG 2 cut(s) 36, 581
AfiI CCNNNNNNNGG 1 cut(s) 299
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 7 cut(s) 62, 338, 415, 478, 610, 689, 702
AjnI CCWGG 2 cut(s) 276, 497
AluBI AGCT 7 cut(s) 11, 158, 356, 430, 488, 559, 629
AluI AGCT 7 cut(s) 11, 158, 356, 430, 488, 559, 629
Alw21I GWGCWC 1 cut(s) 151
AlwI GGATC 2 cut(s) 288, 416
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 1 cut(s) 500
ApeKI GCWGC 2 cut(s) 245, 356
ApoI RAATTY 1 cut(s) 478
AsuC2I CCSGG 2 cut(s) 324, 325
AvaI CYCGRG 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 204
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 257, 343
BccI CCATC 1 cut(s) 524
BciT130I CCWGG 2 cut(s) 278, 499
BciVI GTATCC 1 cut(s) 703
BcnI CCSGG 2 cut(s) 324, 325
BfaI CTAG 2 cut(s) 12, 42
BfrI CTTAAG 1 cut(s) 662
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 2 cut(s) 246, 357
BlsI GCNGC 2 cut(s) 247, 358
Bme1390I CCNGG 4 cut(s) 278, 324, 325, 499
BmeT110I CYCGRG 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 4 cut(s) 278, 324, 325, 499
BmsI GCATC 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 2 cut(s) 189, 360
BpuEI CTTGAG 1 cut(s) 246
BpuMI CCSGG 2 cut(s) 324, 325
BsaJI CCNNGG 5 cut(s) 276, 322, 323, 324, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 231
Bse21I CCTNAGG 1 cut(s) 204
BseBI CCWGG 2 cut(s) 278, 499
BseDI CCNNGG 5 cut(s) 276, 322, 323, 324, 497
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMII CTCAG 4 cut(s) 195, 203, 445, 705
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 2 cut(s) 257, 343
BseYI CCCAGC 1 cut(s) 158
BsgI GTGCAG 2 cut(s) 264, 654
BshFI GGCC 1 cut(s) 502
BsiHKAI GWGCWC 1 cut(s) 151
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 2 cut(s) 52, 239
BslI CCNNNNNNNGG 1 cut(s) 299
BsmFI GGGAC 2 cut(s) 52, 239
BsnI GGCC 1 cut(s) 502
BsoBI CYCGRG 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 280, 421
BspANI GGCC 1 cut(s) 502
BspCNI CTCAG 4 cut(s) 196, 202, 444, 706
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 2 cut(s) 288, 416
BspTI CTTAAG 1 cut(s) 662
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 5 cut(s) 276, 322, 323, 324, 497
BssMI GATC 2 cut(s) 280, 421
BssSI CACGAG 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 278, 499
Bst4CI ACNGT 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 662
BstDEI CTNAG 5 cut(s) 189, 204, 360, 431, 714
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 2 cut(s) 283, 424
BstMBI GATC 2 cut(s) 280, 421
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 2 cut(s) 278, 499
BstSCI CCNGG 4 cut(s) 276, 322, 323, 497
BstV1I GCAGC 2 cut(s) 257, 343
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 1 cut(s) 502
BtsCI GGATG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 323
CviAII CATG 1 cut(s) 103
DdeI CTNAG 5 cut(s) 189, 204, 360, 431, 714
DpnI GATC 2 cut(s) 282, 423
DpnII GATC 2 cut(s) 280, 421
DraI TTTAAA 1 cut(s) 483
Eco57I CTGAAG 2 cut(s) 36, 581
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 276, 497
FaeI CATG 1 cut(s) 106
FaiI YATR 8 cut(s) 21, 104, 317, 582, 593, 617, 641, 726
FalI AAGNNNNNCTT 4 cut(s) 440, 472, 477, 509
FaqI GGGAC 2 cut(s) 52, 239
FatI CATG 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 246, 357
FokI GGATG 1 cut(s) 542
Fsp4HI GCNGC 2 cut(s) 246, 357
FspBI CTAG 2 cut(s) 12, 42
GluI GCNGC 2 cut(s) 246, 357
GsaI CCCAGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 214
HaeIII GGCC 1 cut(s) 502
HapII CCGG 1 cut(s) 324
Hin1II CATG 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 486
HinfI GANTC 5 cut(s) 227, 536, 619, 674, 698
HpaII CCGG 1 cut(s) 324
Hpy188I TCNGA 3 cut(s) 434, 554, 624
Hpy188III TCNNGA 1 cut(s) 610
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 5 cut(s) 130, 442, 556, 725, 743
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 2 cut(s) 245, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 5 cut(s) 189, 204, 360, 431, 714
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 2 cut(s) 280, 421
Lsp1109I GCAGC 2 cut(s) 257, 343
LweI GCATC 1 cut(s) 669
MaeI CTAG 2 cut(s) 12, 42
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 1 cut(s) 600
MalI GATC 2 cut(s) 282, 423
MboI GATC 2 cut(s) 280, 421
MboII GAAGA 5 cut(s) 40, 43, 359, 485, 644
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 4 cut(s) 86, 235, 380, 478
MlyI GAGTC 4 cut(s) 221, 530, 613, 668
MmeI TCCRAC 2 cut(s) 532, 651
MnlI CCTC 5 cut(s) 133, 199, 270, 358, 513
MseI TTAA 3 cut(s) 482, 524, 663
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 158
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 4 cut(s) 278, 324, 325, 499
MvaI CCWGG 2 cut(s) 278, 499
MwoI GCNNNNNNNGC 1 cut(s) 155
NciI CCSGG 2 cut(s) 324, 325
NdeII GATC 2 cut(s) 280, 421
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 1 cut(s) 168
PfeI GAWTC 1 cut(s) 698
PflMI CCANNNNNTGG 1 cut(s) 299
PkrI GCNGC 2 cut(s) 247, 358
PleI GAGTC 4 cut(s) 221, 530, 613, 668
PpsI GAGTC 4 cut(s) 221, 530, 613, 668
Psp6I CCWGG 2 cut(s) 276, 497
PspFI CCCAGC 1 cut(s) 158
PspGI CCWGG 2 cut(s) 276, 497
PspN4I GGNNCC 1 cut(s) 168
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 404
SaqAI TTAA 3 cut(s) 482, 524, 663
SatI GCNGC 2 cut(s) 246, 357
Sau3AI GATC 2 cut(s) 280, 421
SchI GAGTC 4 cut(s) 221, 530, 613, 668
ScrFI CCNGG 4 cut(s) 278, 324, 325, 499
SduI GDGCHC 1 cut(s) 151
SfaNI GCATC 1 cut(s) 669
SmaI CCCGGG 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 2 cut(s) 261, 662
SmoI CTYRAG 2 cut(s) 261, 662
Sse9I AATT 4 cut(s) 86, 235, 380, 478
SspMI CTAG 2 cut(s) 12, 42
StyD4I CCNGG 4 cut(s) 276, 322, 323, 497
TaaI ACNGT 1 cut(s) 443
TaiI ACGT 1 cut(s) 552
TaqI TCGA 2 cut(s) 98, 677
TasI AATT 4 cut(s) 86, 235, 380, 478
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 3 cut(s) 482, 524, 663
Tru9I TTAA 3 cut(s) 482, 524, 663
TscAI CASTG 1 cut(s) 99
TseI GCWGC 2 cut(s) 245, 356
TspDTI ATGAA 1 cut(s) 560
TspMI CCCGGG 1 cut(s) 323
TspRI CASTG 1 cut(s) 99
Van91I CCANNNNNTGG 1 cut(s) 299
Vha464I CTTAAG 1 cut(s) 662
XapI RAATTY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmaI CCCGGG 1 cut(s) 323
XspI CTAG 2 cut(s) 12, 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.