Rh4CG299800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000939
Physical Location & Seq
Forward (+)
56008085 .. 56010680
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG299800.1

Sequence Viewer

Length: 633 bp
ATGGATTTTGTGGGACTTGGGTTTGCTGATAAGAAGGAGGGTAGAGCACTGCCAACTGGGTTGGTTCCAATCGCAGACGATTTTGCTCAGGGCAACTTGCCTGAGGTTGACATTATTGTTGGGGACTCCAGTAATTTCAGTGCAGCCACACCCAAGCCTCAAGGAGATGACCCCCTGGATCTTTACAAGCCAAAGGTTTCTTCATGGGGAGTCTTTCCCAGACCCAATGACATTTCAAAAACGTTTGGTGGTGGGCGAGTTATACACCCCGGGGAAGTGCTTGAAACGCAGGAAGAAAAAGCTGCTAAGAAGGAACGAACAAGACAATTAGTTGCTGCTTACAACACTAAAATGGGCTTGAACATTGATCCCAAGCTCAGATTGGACTGTGAGAAGACATTAAAGGATGGTGACTCATTGATGAACGTCGGAAAGCTGAAGGAAGCATTACCCTACTATGAGAAAGTTATGGATAAGTTACCATTCAAGATTATGACTCCGAAAGCTCGTGCAGACATACACATCAATCTTCCAGCACTTAAGAAGTTGGACTCGATGCTTATTGAAACACTTGATTCAATGGTGGATACTGAGTTTGGGTATGTAGAAGGTGGTAGTAGGGCAGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.03

Weight (kDa)

5.36

Isoelectric Point (pI)

27.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 146 - 208 4.1e-18 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 242
AclWI GGATC 2 cut(s) 186, 362
AcuI CTGAAG 1 cut(s) 458
AflII CTTAAG 1 cut(s) 539
AgsI TTSAA 6 cut(s) 237, 284, 361, 487, 566, 579
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AluBI AGCT 4 cut(s) 302, 376, 436, 506
AluI AGCT 4 cut(s) 302, 376, 436, 506
Alw21I GWGCWC 1 cut(s) 49
AlwI GGATC 2 cut(s) 186, 362
Ama87I CYCGRG 1 cut(s) 269
ApeKI GCWGC 3 cut(s) 143, 302, 335
AsuC2I CCSGG 2 cut(s) 270, 271
AsuHPI GGTGA 1 cut(s) 422
AvaI CYCGRG 1 cut(s) 269
AxyI CCTNAGG 1 cut(s) 102
BauI CACGAG 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 3 cut(s) 155, 289, 322
BccI CCATC 1 cut(s) 401
BciT130I CCWGG 1 cut(s) 176
BciVI GTATCC 1 cut(s) 580
BcnI CCSGG 2 cut(s) 270, 271
BfrI CTTAAG 1 cut(s) 539
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 3 cut(s) 144, 303, 336
BlsI GCNGC 3 cut(s) 145, 304, 337
Bme1390I CCNGG 3 cut(s) 176, 270, 271
BmeT110I CYCGRG 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 3 cut(s) 176, 270, 271
BmrI ACTGGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 546
BmuI ACTGGG 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 112
Bpu10I CCTNAGC 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 144
BpuMI CCSGG 2 cut(s) 270, 271
BsaJI CCNNGG 4 cut(s) 174, 268, 269, 270
Bse1I ACTGG 2 cut(s) 61, 129
Bse21I CCTNAGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 4 cut(s) 174, 268, 269, 270
BseGI GGATG 1 cut(s) 412
BseMII CTCAG 4 cut(s) 93, 101, 391, 582
BseNI ACTGG 2 cut(s) 61, 129
BseXI GCAGC 3 cut(s) 155, 289, 322
BsgI GTGCAG 2 cut(s) 162, 531
BsiHKAI GWGCWC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 2 cut(s) 27, 137
BsmFI GGGAC 2 cut(s) 27, 137
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 178, 367
BspCNI CTCAG 4 cut(s) 94, 100, 390, 583
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 2 cut(s) 186, 362
BspTI CTTAAG 1 cut(s) 539
BsrI ACTGG 2 cut(s) 61, 129
BssECI CCNNGG 4 cut(s) 174, 268, 269, 270
BssMI GATC 2 cut(s) 178, 367
BssSI CACGAG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 389
BstAFI CTTAAG 1 cut(s) 539
BstDEI CTNAG 5 cut(s) 87, 102, 306, 377, 591
BstF5I GGATG 1 cut(s) 412
BstKTI GATC 2 cut(s) 181, 370
BstMBI GATC 2 cut(s) 178, 367
BstMWI GCNNNNNNNGC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 3 cut(s) 174, 268, 269
BstV1I GCAGC 3 cut(s) 155, 289, 322
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 580
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 2 cut(s) 47, 145
Cfr9I CCCGGG 1 cut(s) 269
CviAII CATG 1 cut(s) 204
CviJI RGCY 8 cut(s) 146, 157, 190, 302, 357, 376, 436, 506
CviKI_1 RGCY 8 cut(s) 146, 157, 190, 302, 357, 376, 436, 506
DdeI CTNAG 5 cut(s) 87, 102, 306, 377, 591
DpnI GATC 2 cut(s) 180, 369
DpnII GATC 2 cut(s) 178, 367
Eco57I CTGAAG 1 cut(s) 458
Eco81I CCTNAGG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 174
FaeI CATG 1 cut(s) 207
FaiI YATR 7 cut(s) 205, 263, 459, 470, 494, 518, 603
FaqI GGGAC 2 cut(s) 27, 137
FatI CATG 1 cut(s) 203
Fnu4HI GCNGC 3 cut(s) 144, 303, 336
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 3 cut(s) 144, 303, 336
GluI GCNGC 3 cut(s) 144, 303, 336
GsuI CTGGAG 1 cut(s) 112
HapII CCGG 1 cut(s) 270
Hin1II CATG 1 cut(s) 207
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 6 cut(s) 125, 210, 413, 496, 551, 575
HpaII CCGG 1 cut(s) 270
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 380, 431, 501
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 5 cut(s) 28, 304, 433, 602, 620
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4IV ACGT 2 cut(s) 242, 426
HpyCH4V TGCA 2 cut(s) 143, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 286
HpyF3I CTNAG 5 cut(s) 87, 102, 306, 377, 591
HpySE526I ACGT 2 cut(s) 242, 426
Hsp92II CATG 1 cut(s) 207
Kzo9I GATC 2 cut(s) 178, 367
Lsp1109I GCAGC 3 cut(s) 155, 289, 322
LweI GCATC 1 cut(s) 546
MaeII ACGT 2 cut(s) 242, 426
MaeIII GTNAC 2 cut(s) 410, 477
MalI GATC 2 cut(s) 180, 369
MboI GATC 2 cut(s) 178, 367
MboII GAAGA 4 cut(s) 192, 305, 406, 521
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 2 cut(s) 133, 326
MlyI GAGTC 5 cut(s) 119, 219, 407, 490, 545
MmeI TCCRAC 2 cut(s) 409, 528
MnlI CCTC 3 cut(s) 31, 97, 168
MseI TTAA 2 cut(s) 401, 540
MslI CAYNNNNRTG 1 cut(s) 350
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 3 cut(s) 176, 270, 271
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 286
NciI CCSGG 2 cut(s) 270, 271
NdeII GATC 2 cut(s) 178, 367
NlaIII CATG 1 cut(s) 207
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 575
PkrI GCNGC 3 cut(s) 145, 304, 337
PleI GAGTC 5 cut(s) 119, 218, 407, 490, 545
PpsI GAGTC 5 cut(s) 119, 218, 407, 490, 545
Psp1406I AACGTT 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 66
PsuI RGATCY 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 401, 540
SatI GCNGC 3 cut(s) 144, 303, 336
Sau3AI GATC 2 cut(s) 178, 367
SchI GAGTC 5 cut(s) 119, 219, 407, 490, 545
ScrFI CCNGG 3 cut(s) 176, 270, 271
SduI GDGCHC 1 cut(s) 49
SetI ASST 9 cut(s) 108, 198, 245, 304, 378, 429, 438, 508, 613
SfaNI GCATC 1 cut(s) 546
SmaI CCCGGG 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 159, 539
SmoI CTYRAG 2 cut(s) 159, 539
Sse9I AATT 2 cut(s) 133, 326
StyD4I CCNGG 3 cut(s) 174, 268, 269
TaaI ACNGT 1 cut(s) 389
TaiI ACGT 2 cut(s) 245, 429
TaqI TCGA 1 cut(s) 554
TasI AATT 2 cut(s) 133, 326
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 2 cut(s) 401, 540
Tru9I TTAA 2 cut(s) 401, 540
TscAI CASTG 2 cut(s) 54, 145
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 3 cut(s) 143, 302, 335
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 2 cut(s) 192, 437
TspMI CCCGGG 1 cut(s) 269
TspRI CASTG 2 cut(s) 54, 145
Vha464I CTTAAG 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XmaI CCCGGG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.