FvH4_5g33630

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001684
Physical Location & Seq
Reverse (-)
24352375 .. 24357930
5556 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33630.t2

Sequence Viewer

Length: 1509 bp
ATGAGAAAACTAAACCAAATCCATGCGCTGCTCATCAAAAATCCAAAACCCCAAGTTTTAAACCCCTGGCTTGGATTTCTGACAAATTCTTCAGCACCACAAAATGCCCTTCTTCTTTATAACCAAATGCTCCACCACCCAACTTCCCATAACCACTACACATTTACTTATGCTCTTAAGGCATGCTGCTTGCTCCATTCACCTCATAAAGGCCAAGAAATCCAAGCCCACGTCACAAAATCTGGTCACATATCTGATACCTTCATCCAAAACTCCTTGCTCCATTTCTACGTAATCCAATCTGACATTGTTTCTGCTGCCCATGTTTTTGATTCGATACCTTTACCGGATGTTGTTTCCTGGACTTCAATGATTTCGGGTCTTGCCAAGTGTGGGTTTGTGGACGAAGCAATTGTGAAGTTTGTGTCCATGGACGTAAAGCCTAATCCTACTACTCTTGTGACTGTGTTGTCTTCGTGTTCTACTTTAAGAGCTGTCAAGTTTGGTAAAGCTGTTCATGGCCATTGTTTGAGGAACTTTCATGAAAGTAATTTGATTTTGGACAATGCCGTTTTGGATTTCTATCTGAGATGTGGATCTTTGGCTAGTGCAAGATATCTGTTTGTCAACATGCCCAAGAGAGATGTCATTTCTTGGACTAGCATGGTGGGTGGTTATGCACAAAGAGGTTTTTGTGAAGAGGCAGTGAAACTTTTCCAACAGATGGTGCTGGGCGGGGAAGCTGAGCCTAATGAGGCTACCATTGTGAATGTATTGTCAGCATGTTCTTCGATTTGTGCATTGAGTTCAGGCCAGCATGTGCATTTCTATATCAGTACTCGACGAGATCTCATGGCAAATGTCAATGTGGGAAATGCCTTGATGAACATGTATGTCAAGTGTGGAAATTTGGGTATGGCTATTTCGGTTTTTAAGGCCCTGGAGTGCAAAGATATCATTTCATGGACTACTATAATAAGTGGCATGGCCATGAATGGCCATGGCATCCATGTGTTGCAGCTCTTTTCCAGTATGCTAGTTAATGGGGTTTCTCCTGATGGGGTAACCTTCCTTGGGTTGTTAACAGCATGCAGTCATGCTGGCCTGGTAGATCAAGGGTTTATGTTCTTTGATGTAATGAAGAAAATTTACCGGATTGTCCCTCAAACACAGCATTATGCGTGTCTGGTTGATATGTATGGTCGAGCTGGCCTTTTAGAGGAAGCAGAGGCTCTTATTAAAGAATTGCCAATGGAAGCTGATGGTCCTGTTTGGGGGGCACTGCTTAATGCGTGCAAAATTCATGGGAATGAGGAGATGGTTGAGAGGATCAAAGAAGGCCTCCACAATAGTGCACAAGTGAGCATTGGAACTTATGCGCTGTTATCAAATGCTTATGCTAAACATGATAGGTGGGATGATTCTAATAAGGTTCGCGATGAAATGAGAGAGATGGGGTTGAAGACACCACCTGGGTGTAGTTGGATTGAGATTGGTCACTCGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

503

Amino Acids

55.4

Weight (kDa)

6.91

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 116 - 144 4e-06 PPR repeat
PPR_2 PF13041 214 - 262 1e-05 PPR repeat family
PPR PF01535 217 - 243 6.3e-07 PPR repeat
PPR_2 PF13041 317 - 365 1.4e-08 PPR repeat family
E_motif PF20431 453 - 497 8.4e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 120
AasI GACNNNNNNGTC 1 cut(s) 469
AccB7I CCANNNNNTGG 1 cut(s) 724
AccII CGCG 1 cut(s) 1437
AciI CCGC 1 cut(s) 735
AclWI GGATC 2 cut(s) 604, 1337
AcoI YGGCCR 3 cut(s) 520, 987, 997
AcsI RAATTY 4 cut(s) 85, 907, 1146, 1299
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 1 cut(s) 838
AfiI CCNNNNNNNGG 7 cut(s) 71, 209, 393, 724, 1074, 1219, 1274
AflII CTTAAG 1 cut(s) 176
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 2 cut(s) 369, 1462
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 5 cut(s) 65, 359, 939, 1104, 1471
AleI CACNNNNGTG 2 cut(s) 1350, 1474
AluBI AGCT 6 cut(s) 494, 512, 743, 1021, 1208, 1259
AluI AGCT 6 cut(s) 494, 512, 743, 1021, 1208, 1259
Alw21I GWGCWC 1 cut(s) 1357
Alw44I GTGCAC 1 cut(s) 1353
AlwI GGATC 2 cut(s) 604, 1337
AoxI GGCC 9 cut(s) 211, 520, 811, 936, 987, 997, 1102, 1210, 1339
ApaLI GTGCAC 1 cut(s) 1353
ApeKI GCWGC 4 cut(s) 28, 186, 317, 1018
ApoI RAATTY 4 cut(s) 85, 907, 1146, 1299
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 2 cut(s) 28, 1381
AspS9I GGNCC 2 cut(s) 937, 1265
AsuHPI GGTGA 1 cut(s) 192
AvaII GGWCC 1 cut(s) 1265
BaeGI GKGCMC 2 cut(s) 1282, 1357
BalI TGGCCA 3 cut(s) 522, 989, 999
BarI GAAGNNNNNNTAC 2 cut(s) 1133, 1165
BbsI GAAGAC 2 cut(s) 465, 1469
Bbv12I GWGCWC 1 cut(s) 1357
BbvI GCAGC 4 cut(s) 15, 173, 304, 1030
BccI CCATC 5 cut(s) 718, 1052, 1256, 1312, 1447
BceAI ACGGC 1 cut(s) 554
BcgI CGANNNNNNTGC 2 cut(s) 771, 805
BciT130I CCWGG 5 cut(s) 67, 361, 941, 1106, 1473
BfaI CTAG 3 cut(s) 606, 660, 1037
BfrI CTTAAG 1 cut(s) 176
BglII AGATCT 1 cut(s) 847
BisI GCNGC 4 cut(s) 29, 187, 318, 1019
BlpI GCTNAGC 1 cut(s) 744
BlsI GCNGC 4 cut(s) 30, 188, 319, 1020
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 5 cut(s) 67, 361, 941, 1106, 1473
Bme18I GGWCC 1 cut(s) 1265
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 2 cut(s) 937, 1265
BmrFI CCNGG 5 cut(s) 67, 361, 941, 1106, 1473
BmsI GCATC 1 cut(s) 1014
BpiI GAAGAC 2 cut(s) 465, 1469
BpmI CTGGAG 1 cut(s) 962
Bpu1102I GCTNAGC 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 292
BsaBI GATNNNNATC 2 cut(s) 582, 595
BsaJI CCNNGG 6 cut(s) 65, 429, 939, 1000, 1072, 1472
BsaWI WCCGGW 2 cut(s) 346, 1152
Bsc4I CCNNNNNNNGG 7 cut(s) 71, 209, 393, 724, 1074, 1219, 1274
Bse1I ACTGG 1 cut(s) 1029
Bse8I GATNNNNATC 2 cut(s) 582, 595
BseBI CCWGG 5 cut(s) 67, 361, 941, 1106, 1473
BseDI CCNNGG 6 cut(s) 65, 429, 939, 1000, 1072, 1472
BseGI GGATG 4 cut(s) 264, 355, 1005, 1423
BseJI GATNNNNATC 2 cut(s) 582, 595
BseLI CCNNNNNNNGG 7 cut(s) 71, 209, 393, 724, 1074, 1219, 1274
BseMII CTCAG 2 cut(s) 578, 735
BseNI ACTGG 1 cut(s) 1029
BseRI GAGGAG 1 cut(s) 1328
BseSI GKGCMC 2 cut(s) 1282, 1357
BseXI GCAGC 4 cut(s) 15, 173, 304, 1030
BseYI CCCAGC 1 cut(s) 730
Bsh1236I CGCG 1 cut(s) 1437
BshFI GGCC 9 cut(s) 213, 522, 813, 938, 989, 999, 1104, 1212, 1341
BsiHKAI GWGCWC 1 cut(s) 1357
BsiSI CCGG 2 cut(s) 347, 1153
BslFI GGGAC 1 cut(s) 1145
BslI CCNNNNNNNGG 7 cut(s) 71, 209, 393, 724, 1074, 1219, 1274
BsmFI GGGAC 1 cut(s) 1145
BsnI GGCC 9 cut(s) 213, 522, 813, 938, 989, 999, 1104, 1212, 1341
Bsp1286I GDGCHC 2 cut(s) 1282, 1357
Bsp143I GATC 5 cut(s) 596, 847, 1111, 1329, 1503
Bsp1720I GCTNAGC 1 cut(s) 744
Bsp19I CCATGG 2 cut(s) 429, 1000
Bsp68I TCGCGA 1 cut(s) 1437
BspACI CCGC 1 cut(s) 735
BspANI GGCC 9 cut(s) 213, 522, 813, 938, 989, 999, 1104, 1212, 1341
BspCNI CTCAG 2 cut(s) 579, 736
BspFNI CGCG 1 cut(s) 1437
BspHI TCATGA 1 cut(s) 541
BspPI GGATC 2 cut(s) 604, 1337
BspTI CTTAAG 1 cut(s) 176
BsrI ACTGG 1 cut(s) 1029
BssECI CCNNGG 6 cut(s) 65, 429, 939, 1000, 1072, 1472
BssMI GATC 5 cut(s) 596, 847, 1111, 1329, 1503
BssT1I CCWWGG 3 cut(s) 429, 1000, 1072
Bst2UI CCWGG 5 cut(s) 67, 361, 941, 1106, 1473
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 693
BstAFI CTTAAG 1 cut(s) 176
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 7 cut(s) 184, 191, 815, 1090, 1102, 1210, 1294
BstDEI CTNAG 2 cut(s) 587, 744
BstDSI CCRYGG 2 cut(s) 429, 1000
BstEII GGTNACC 1 cut(s) 1063
BstENI CCTNNNNNAGG 2 cut(s) 207, 1217
BstF5I GGATG 4 cut(s) 264, 355, 1005, 1423
BstFNI CGCG 1 cut(s) 1437
BstHHI GCGC 2 cut(s) 28, 1381
BstKTI GATC 5 cut(s) 599, 850, 1114, 1332, 1506
BstMBI GATC 5 cut(s) 596, 847, 1111, 1329, 1503
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 5 cut(s) 67, 361, 941, 1106, 1473
BstNSI RCATGY 6 cut(s) 186, 634, 786, 821, 892, 1092
BstPI GGTNACC 1 cut(s) 1063
BstSCI CCNGG 5 cut(s) 65, 359, 939, 1104, 1471
BstSLI GKGCMC 2 cut(s) 1282, 1357
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 1 cut(s) 1437
BstV1I GCAGC 4 cut(s) 15, 173, 304, 1030
BstV2I GAAGAC 2 cut(s) 465, 1469
BstX2I RGATCY 2 cut(s) 596, 847
BstYI RGATCY 2 cut(s) 596, 847
BsuRI GGCC 9 cut(s) 213, 522, 813, 938, 989, 999, 1104, 1212, 1341
BtgI CCRYGG 2 cut(s) 429, 1000
BtgZI GCGATG 1 cut(s) 1452
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 4 cut(s) 264, 355, 1005, 1423
BtsI GCAGTG 2 cut(s) 711, 1280
BtsIMutI CAGTG 2 cut(s) 711, 1280
BtuMI TCGCGA 1 cut(s) 1437
Cac8I GCNNGC 7 cut(s) 184, 191, 815, 1090, 1102, 1210, 1294
CciI TCATGA 1 cut(s) 541
CfoI GCGC 2 cut(s) 28, 1381
Cfr13I GGNCC 2 cut(s) 937, 1265
Csp6I GTAC 1 cut(s) 837
CviQI GTAC 1 cut(s) 837
DdeI CTNAG 2 cut(s) 587, 744
DpnI GATC 5 cut(s) 598, 849, 1113, 1331, 1505
DpnII GATC 5 cut(s) 596, 847, 1111, 1329, 1503
DraI TTTAAA 1 cut(s) 60
DrdI GACNNNNNNGTC 1 cut(s) 469
DseDI GACNNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 3 cut(s) 520, 987, 997
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 3 cut(s) 429, 1000, 1072
Eco147I AGGCCT 1 cut(s) 1341
Eco32I GATATC 2 cut(s) 617, 955
Eco47I GGWCC 1 cut(s) 1265
Eco57I CTGAAG 1 cut(s) 75
Eco91I GGTNACC 1 cut(s) 1063
EcoNI CCTNNNNNAGG 2 cut(s) 207, 1217
EcoO109I RGGNCCY 1 cut(s) 937
EcoO65I GGTNACC 1 cut(s) 1063
EcoRII CCWGG 5 cut(s) 65, 359, 939, 1104, 1471
EcoRV GATATC 2 cut(s) 617, 955
EcoT14I CCWWGG 3 cut(s) 429, 1000, 1072
ErhI CCWWGG 3 cut(s) 429, 1000, 1072
FaqI GGGAC 1 cut(s) 1145
FauI CCCGC 1 cut(s) 728
Fnu4HI GCNGC 4 cut(s) 29, 187, 318, 1019
FokI GGATG 4 cut(s) 251, 362, 992, 1430
Fsp4HI GCNGC 4 cut(s) 29, 187, 318, 1019
FspBI CTAG 3 cut(s) 606, 660, 1037
GlaI GCGC 2 cut(s) 27, 1380
GluI GCNGC 4 cut(s) 29, 187, 318, 1019
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 962
HaeIII GGCC 9 cut(s) 213, 522, 813, 938, 989, 999, 1104, 1212, 1341
HapII CCGG 2 cut(s) 347, 1153
HhaI GCGC 2 cut(s) 28, 1381
Hin6I GCGC 2 cut(s) 26, 1379
HinP1I GCGC 2 cut(s) 26, 1379
HincII GTYRAC 2 cut(s) 628, 1083
HindII GTYRAC 2 cut(s) 628, 1083
HinfI GANTC 2 cut(s) 332, 1421
HpaI GTTAAC 1 cut(s) 1083
HpaII CCGG 2 cut(s) 347, 1153
HphI GGTGA 1 cut(s) 192
Hpy166II GTNNAC 4 cut(s) 403, 628, 1083, 1355
Hpy188I TCNGA 4 cut(s) 81, 256, 304, 588
Hpy188III TCNNGA 3 cut(s) 542, 1055, 1436
Hpy8I GTNNAC 4 cut(s) 403, 628, 1083, 1355
Hpy99I CGWCG 1 cut(s) 846
HpyAV CCTTC 4 cut(s) 119, 271, 1078, 1331
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 3 cut(s) 231, 291, 435
HpyCH4V TGCA 9 cut(s) 611, 680, 800, 823, 948, 1018, 1092, 1296, 1355
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 2 cut(s) 587, 744
HpySE526I ACGT 3 cut(s) 231, 291, 435
HspAI GCGC 2 cut(s) 26, 1379
KspAI GTTAAC 1 cut(s) 1083
Kzo9I GATC 5 cut(s) 596, 847, 1111, 1329, 1503
LmnI GCTCC 3 cut(s) 135, 198, 285
Lsp1109I GCAGC 4 cut(s) 15, 173, 304, 1030
LweI GCATC 1 cut(s) 1014
MaeI CTAG 3 cut(s) 606, 660, 1037
MaeII ACGT 3 cut(s) 231, 291, 435
MaeIII GTNAC 5 cut(s) 232, 245, 460, 1063, 1496
MalI GATC 5 cut(s) 598, 849, 1113, 1331, 1505
MboI GATC 5 cut(s) 596, 847, 1111, 1329, 1503
MboII GAAGA 7 cut(s) 81, 104, 465, 710, 780, 1153, 1474
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 2 cut(s) 596, 847
MhlI GDGCHC 2 cut(s) 1282, 1357
MlsI TGGCCA 3 cut(s) 522, 989, 999
MluCI AATT 7 cut(s) 85, 411, 550, 907, 1146, 1244, 1299
MluNI TGGCCA 3 cut(s) 522, 989, 999
MmeI TCCRAC 2 cut(s) 742, 1463
Mox20I TGGCCA 3 cut(s) 522, 989, 999
MroXI GAANNNNTTC 1 cut(s) 713
MscI TGGCCA 3 cut(s) 522, 989, 999
MseI TTAA 8 cut(s) 59, 177, 488, 933, 1041, 1082, 1239, 1287
MslI CAYNNNNRTG 5 cut(s) 989, 1010, 1308, 1350, 1474
Msp20I TGGCCA 3 cut(s) 522, 989, 999
MspCI CTTAAG 1 cut(s) 176
MspI CCGG 2 cut(s) 347, 1153
MspR9I CCNGG 5 cut(s) 67, 361, 941, 1106, 1473
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 5 cut(s) 67, 361, 941, 1106, 1473
MvnI CGCG 1 cut(s) 1437
MwoI GCNNNNNNNGC 1 cut(s) 179
NcoI CCATGG 2 cut(s) 429, 1000
NdeII GATC 5 cut(s) 596, 847, 1111, 1329, 1503
NmuCI GTSAC 4 cut(s) 232, 245, 460, 1496
NruI TCGCGA 1 cut(s) 1437
NspI RCATGY 6 cut(s) 186, 634, 786, 821, 892, 1092
OliI CACNNNNGTG 2 cut(s) 1350, 1474
PaeI GCATGC 2 cut(s) 186, 1092
PagI TCATGA 1 cut(s) 541
PceI AGGCCT 1 cut(s) 1341
PciI ACATGT 1 cut(s) 888
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 332, 1421
PflMI CCANNNNNTGG 1 cut(s) 724
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 4 cut(s) 30, 188, 319, 1020
Ppu21I YACGTR 1 cut(s) 292
PscI ACATGT 1 cut(s) 888
PsiI TTATAA 1 cut(s) 120
Psp6I CCWGG 5 cut(s) 65, 359, 939, 1104, 1471
PspEI GGTNACC 1 cut(s) 1063
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 5 cut(s) 65, 359, 939, 1104, 1471
PspPI GGNCC 2 cut(s) 937, 1265
PsuI RGATCY 2 cut(s) 596, 847
RruI TCGCGA 1 cut(s) 1437
RsaI GTAC 1 cut(s) 838
RsaNI GTAC 1 cut(s) 837
RseI CAYNNNNRTG 5 cut(s) 989, 1010, 1308, 1350, 1474
SaqAI TTAA 8 cut(s) 59, 177, 488, 933, 1041, 1082, 1239, 1287
SatI GCNGC 4 cut(s) 29, 187, 318, 1019
Sau3AI GATC 5 cut(s) 596, 847, 1111, 1329, 1503
Sau96I GGNCC 2 cut(s) 937, 1265
ScaI AGTACT 1 cut(s) 838
ScrFI CCNGG 5 cut(s) 67, 361, 941, 1106, 1473
SduI GDGCHC 2 cut(s) 1282, 1357
SfaNI GCATC 1 cut(s) 1014
SinI GGWCC 1 cut(s) 1265
SmiMI CAYNNNNRTG 5 cut(s) 989, 1010, 1308, 1350, 1474
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SnaBI TACGTA 1 cut(s) 292
SphI GCATGC 2 cut(s) 186, 1092
Sse9I AATT 7 cut(s) 85, 411, 550, 907, 1146, 1244, 1299
SseBI AGGCCT 1 cut(s) 1341
SsiI CCGC 1 cut(s) 735
SspMI CTAG 3 cut(s) 606, 660, 1037
StuI AGGCCT 1 cut(s) 1341
StyD4I CCNGG 5 cut(s) 65, 359, 939, 1104, 1471
StyI CCWWGG 3 cut(s) 429, 1000, 1072
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 3 cut(s) 234, 294, 438
TaqI TCGA 5 cut(s) 335, 791, 841, 1204, 1502
TasI AATT 7 cut(s) 85, 411, 550, 907, 1146, 1244, 1299
TatI WGTACW 1 cut(s) 836
TfiI GAWTC 2 cut(s) 332, 1421
Tru1I TTAA 8 cut(s) 59, 177, 488, 933, 1041, 1082, 1239, 1287
Tru9I TTAA 8 cut(s) 59, 177, 488, 933, 1041, 1082, 1239, 1287
TscAI CASTG 2 cut(s) 711, 1287
TseFI GTSAC 4 cut(s) 232, 245, 460, 1496
TseI GCWGC 4 cut(s) 28, 186, 317, 1018
Tsp45I GTSAC 4 cut(s) 232, 245, 460, 1496
TspRI CASTG 2 cut(s) 711, 1287
Van91I CCANNNNNTGG 1 cut(s) 724
Vha464I CTTAAG 1 cut(s) 176
VneI GTGCAC 1 cut(s) 1353
VpaK11BI GGWCC 1 cut(s) 1265
XagI CCTNNNNNAGG 2 cut(s) 207, 1217
XapI RAATTY 4 cut(s) 85, 907, 1146, 1299
XceI RCATGY 6 cut(s) 186, 634, 786, 821, 892, 1092
XmnI GAANNNNTTC 1 cut(s) 713
XspI CTAG 3 cut(s) 606, 660, 1037
ZrmI AGTACT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.