pycom03g13880

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001684
Physical Location & Seq
Reverse (-)
14985621 .. 14987123
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13880.1

Sequence Viewer

Length: 1503 bp
ATGAAACGACAATTAAAGCAAATCATCAAAAACCCTAAACCCCAACTGTTAAACCCTCTACTGGGACATCTCACAAACTCCCCCACCCCGCAAACTGCCCTTTTCCTCTACAACCAAATGCTCCACTACCCAACCTCCCACAACCACTACACCTTCACTTACGCTCTCAAGGCATGTTGCTTCCTCCATGATGACAACAAGGGCCGGGAAGTCCACGCCCGCGTCATAAAAACCGGGCACTTTTCCGATACCTTCATCCAAAACTCGTTGCTTCACTTCTACGTAACCCAAGCTGACATTGCATCTGCTACCCGGGTTTTCAATTCCATACCAAAACCTGATGTTGTTTCGTGGACTTCGATGATTTCGGGTCTTAACAAGTGTGGATTTGTGGAGGAAGCAATTGTTAAGTTTATGTCCATGGATGTGCCGCCTAATTCTACTACTCTTGTCATTGTTATGTCCGCTTGTTCGAGTTTAGGAGCCTTAAAGTTTGGTAAATCTGTTCATGGATATTGTATGAGGAAGTTATCTGAAAGGAATGTTATTTTGGAGAATGCAGTGTTGGATTTTTACATGAGATGTGGGTCTTTGGTGAGTGCACGGTACCTGTTTGTAAATATGCCCAAGAGAGATGTGTATTCTTGGACTAGCATGGTGGGTGGTTATGCACAAAGAGGATTTTGTGATGAGGCAGTGAGGCTTTTTCAGGAGATGGTTCAGGGAGACGAAGTTGAGCCTAATGAGGCTACCATTGTAAATGTATTGTCAGCGTGTTCTTCGATTGGGGCATTGAGTTTAGGCCAGTGGGTGAATTCGTATATAAGTGCACGGCCAGATCTCGTGTTGAATGGCATTGTGGGAAATGCATTAATAAACATGTATGTAAAGTGTGGTGAAGTAGGTATGGCAGTTTCGGTTTTTAAAACCCTGGTTTGCAAGGATAACATTTCATGGAGTACTATCATAAGTGGCATGGCCATGAATGGTTATGGTATACACGCGTTGCAGCTCTTTTCACTCATGCTAGTCCACGGGATTCCTCCTGATGATGTAACCTTCCTTGGCTTGTTATCAGCATGCAGTCACACAGGTCTAGTCGATCAAGGATTGATGATTTTCAATGCTATGAAAAATGTTTATCGGATTGCCCCTCAAACACAGCATTACGCCTGCCTAGTTGATATGTATGGTCGAGCGGGGTTCTTGGAGGAAGCAGAGAGTTTCATGAAGGAAATGCCAACAGAAGCAGACGGCGCTGTTTGGGGAGCGTTGCTTAATGCATGCAAAATTCATGGGAATGAGAAGATGTTTAAGAGGATCAGAAATGGTCTCCTCAAGAGTAGAGGAGTAAGCATCGGAACTTATGCTCTTGTATCGAATACATACACCACTCACGAACGGTGGGATGAGGCTGATAAGGTTCGTGATGAAATGCGACAGATGGGGTTGAAAAAATTAGCAGCATATAGTCAGATAGATGTCGATCCAAATATCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

501

Amino Acids

55.62

Weight (kDa)

8.31

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 211 - 259 1.3e-06 PPR repeat family
PPR PF01535 214 - 241 3.1e-07 PPR repeat
PPR_2 PF13041 315 - 362 1.1e-07 PPR repeat family
E_motif PF20431 454 - 491 3.1e-06 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1501
Acc65I GGTACC 1 cut(s) 606
AccB1I GGYRCC 1 cut(s) 606
AccBSI CCGCTC 1 cut(s) 1199
AccI GTMKAC 1 cut(s) 997
AccII CGCG 2 cut(s) 222, 1004
AciI CCGC 5 cut(s) 89, 220, 431, 465, 1199
AclWI GGATC 2 cut(s) 1328, 1481
AcoI YGGCCR 2 cut(s) 833, 978
AcsI RAATTY 2 cut(s) 814, 1290
AfaI GTAC 2 cut(s) 608, 961
AfiI CCNNNNNNNGG 2 cut(s) 61, 62
AflIII ACRYGT 2 cut(s) 879, 1002
AgsI TTSAA 4 cut(s) 322, 850, 1123, 1453
AjnI CCWGG 1 cut(s) 930
AjuI GAANNNNNNNTTGG 4 cut(s) 533, 548, 565, 580
AluBI AGCT 2 cut(s) 293, 1012
AluI AGCT 2 cut(s) 293, 1012
Alw21I GWGCWC 2 cut(s) 604, 832
Alw26I GTCTC 2 cut(s) 720, 1337
Alw44I GTGCAC 2 cut(s) 600, 828
AlwI GGATC 2 cut(s) 1328, 1481
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 4 cut(s) 202, 802, 833, 978
ApaLI GTGCAC 2 cut(s) 600, 828
ApeKI GCWGC 2 cut(s) 1009, 1463
ApoI RAATTY 2 cut(s) 814, 1290
AseI ATTAAT 1 cut(s) 872
Asp718I GGTACC 1 cut(s) 606
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 4 cut(s) 206, 235, 313, 314
AsuHPI GGTGA 3 cut(s) 607, 823, 908
AvaI CYCGRG 1 cut(s) 312
BaeGI GKGCMC 3 cut(s) 240, 604, 832
BalI TGGCCA 1 cut(s) 980
BanI GGYRCC 1 cut(s) 606
BauI CACGAG 1 cut(s) 842
Bbv12I GWGCWC 2 cut(s) 604, 832
BbvI GCAGC 2 cut(s) 1021, 1475
BccI CCATC 2 cut(s) 709, 1438
BceAI ACGGC 2 cut(s) 848, 1270
BciT130I CCWGG 1 cut(s) 932
BcnI CCSGG 4 cut(s) 206, 235, 313, 314
BcoDI GTCTC 2 cut(s) 720, 1337
BfaI CTAG 4 cut(s) 651, 1028, 1097, 1178
BfoI RGCGCY 1 cut(s) 1260
BglII AGATCT 1 cut(s) 838
BisI GCNGC 3 cut(s) 431, 1010, 1464
BlsI GCNGC 3 cut(s) 432, 1011, 1465
BmcAI AGTACT 1 cut(s) 961
Bme1390I CCNGG 5 cut(s) 206, 235, 313, 314, 932
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 484, 608
BmrFI CCNGG 5 cut(s) 206, 235, 313, 314, 932
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 311, 1365
BmuI ACTGGG 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 152, 1322
BpuMI CCSGG 4 cut(s) 206, 235, 313, 314
BsaAI YACGTR 1 cut(s) 283
BsaBI GATNNNNATC 1 cut(s) 1485
BsaI GGTCTC 1 cut(s) 1337
BsaJI CCNNGG 5 cut(s) 312, 420, 930, 1033, 1063
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 717, 747
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 62
Bse1I ACTGG 2 cut(s) 66, 805
Bse3DI GCAATG 1 cut(s) 297
Bse8I GATNNNNATC 1 cut(s) 1485
BseBI CCWGG 1 cut(s) 932
BseDI CCNNGG 5 cut(s) 312, 420, 930, 1033, 1063
BseGI GGATG 3 cut(s) 255, 430, 1414
BseJI GATNNNNATC 1 cut(s) 1485
BseLI CCNNNNNNNGG 2 cut(s) 61, 62
BseMI GCAATG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 66, 805
BseRI GAGGAG 2 cut(s) 1325, 1362
BseSI GKGCMC 3 cut(s) 240, 604, 832
BseXI GCAGC 2 cut(s) 1021, 1475
Bsh1236I CGCG 2 cut(s) 222, 1004
BshFI GGCC 4 cut(s) 204, 804, 835, 980
BshNI GGYRCC 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 604, 832
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 205, 234, 313
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 2 cut(s) 61, 62
BsmAI GTCTC 2 cut(s) 720, 1337
BsmBI CGTCTC 1 cut(s) 720
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 4 cut(s) 204, 804, 835, 980
Bso31I GGTCTC 1 cut(s) 1337
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 3 cut(s) 240, 604, 832
Bsp143I GATC 4 cut(s) 838, 1102, 1320, 1486
Bsp19I CCATGG 1 cut(s) 420
BspACI CCGC 5 cut(s) 89, 220, 431, 465, 1199
BspANI GGCC 4 cut(s) 204, 804, 835, 980
BspFNI CGCG 2 cut(s) 222, 1004
BspHI TCATGA 1 cut(s) 1227
BspLI GGNNCC 2 cut(s) 484, 608
BspPI GGATC 2 cut(s) 1328, 1481
BspT107I GGYRCC 1 cut(s) 606
BspTNI GGTCTC 1 cut(s) 1337
BsrBI CCGCTC 1 cut(s) 1199
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 2 cut(s) 66, 805
BssECI CCNNGG 5 cut(s) 312, 420, 930, 1033, 1063
BssMI GATC 4 cut(s) 838, 1102, 1320, 1486
BssNAI GTATAC 1 cut(s) 998
BssSI CACGAG 1 cut(s) 842
BssT1I CCWWGG 2 cut(s) 420, 1063
Bst1107I GTATAC 1 cut(s) 998
Bst2BI CACGAG 1 cut(s) 842
Bst2UI CCWGG 1 cut(s) 932
Bst4CI ACNGT 3 cut(s) 48, 606, 1404
BstBAI YACGTR 1 cut(s) 283
BstC8I GCNNGC 4 cut(s) 220, 1081, 1174, 1285
BstDSI CCRYGG 2 cut(s) 420, 1033
BstF5I GGATG 3 cut(s) 255, 430, 1414
BstFNI CGCG 2 cut(s) 222, 1004
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 4 cut(s) 841, 1105, 1323, 1489
BstMAI GTCTC 2 cut(s) 720, 1337
BstMBI GATC 4 cut(s) 838, 1102, 1320, 1486
BstMWI GCNNNNNNNGC 3 cut(s) 170, 299, 1256
BstNI CCWGG 1 cut(s) 932
BstNSI RCATGY 4 cut(s) 177, 883, 1083, 1287
BstSCI CCNGG 5 cut(s) 204, 233, 311, 312, 930
BstSLI GKGCMC 3 cut(s) 240, 604, 832
BstSNI TACGTA 1 cut(s) 283
BstUI CGCG 2 cut(s) 222, 1004
BstV1I GCAGC 2 cut(s) 1021, 1475
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BstZ17I GTATAC 1 cut(s) 998
BsuRI GGCC 4 cut(s) 204, 804, 835, 980
BtgI CCRYGG 2 cut(s) 420, 1033
BtsCI GGATG 3 cut(s) 255, 430, 1414
BtsI GCAGTG 2 cut(s) 567, 702
BtsIMutI CAGTG 3 cut(s) 567, 702, 812
Cac8I GCNNGC 4 cut(s) 220, 1081, 1174, 1285
CciI TCATGA 1 cut(s) 1227
CfoI GCGC 1 cut(s) 1259
Cfr13I GGNCC 1 cut(s) 202
Cfr9I CCCGGG 1 cut(s) 312
CseI GACGC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 607, 960
CviQI GTAC 2 cut(s) 607, 960
DpnI GATC 4 cut(s) 840, 1104, 1322, 1488
DpnII GATC 4 cut(s) 838, 1102, 1320, 1486
DraI TTTAAA 1 cut(s) 925
EaeI YGGCCR 2 cut(s) 833, 978
Eco105I TACGTA 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 420, 1063
Eco31I GGTCTC 1 cut(s) 1337
Eco88I CYCGRG 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 930
EcoT14I CCWWGG 2 cut(s) 420, 1063
EcoT22I ATGCAT 2 cut(s) 871, 1285
ErhI CCWWGG 2 cut(s) 420, 1063
Esp3I CGTCTC 1 cut(s) 720
FaqI GGGAC 1 cut(s) 78
FauI CCCGC 3 cut(s) 96, 227, 1192
FblI GTMKAC 1 cut(s) 997
Fnu4HI GCNGC 3 cut(s) 431, 1010, 1464
FokI GGATG 3 cut(s) 242, 437, 1421
Fsp4HI GCNGC 3 cut(s) 431, 1010, 1464
FspBI CTAG 4 cut(s) 651, 1028, 1097, 1178
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 3 cut(s) 431, 1010, 1464
HaeII RGCGCY 1 cut(s) 1260
HaeIII GGCC 4 cut(s) 204, 804, 835, 980
HapII CCGG 3 cut(s) 205, 234, 313
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 1259
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HinfI GANTC 1 cut(s) 1039
HpaII CCGG 3 cut(s) 205, 234, 313
HphI GGTGA 3 cut(s) 607, 823, 908
Hpy166II GTNNAC 6 cut(s) 214, 354, 602, 830, 998, 1033
Hpy188I TCNGA 6 cut(s) 247, 535, 1146, 1325, 1361, 1476
Hpy188III TCNNGA 6 cut(s) 710, 1046, 1228, 1339, 1397, 1427
Hpy8I GTNNAC 6 cut(s) 214, 354, 602, 830, 998, 1033
HpyAV CCTTC 4 cut(s) 163, 262, 1069, 1225
HpyCH4III ACNGT 3 cut(s) 48, 606, 1404
HpyCH4IV ACGT 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 299, 1256
HpySE526I ACGT 1 cut(s) 282
HspAI GCGC 1 cut(s) 1257
KpnI GGTACC 1 cut(s) 610
Kzo9I GATC 4 cut(s) 838, 1102, 1320, 1486
LmnI GCTCC 3 cut(s) 126, 482, 1268
Lsp1109I GCAGC 2 cut(s) 1021, 1475
LweI GCATC 2 cut(s) 311, 1365
MaeI CTAG 4 cut(s) 651, 1028, 1097, 1178
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 3 cut(s) 283, 1054, 1085
MalI GATC 4 cut(s) 840, 1104, 1322, 1488
MbiI CCGCTC 1 cut(s) 1199
MboI GATC 4 cut(s) 838, 1102, 1320, 1486
MboII GAAGA 2 cut(s) 771, 1318
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 3 cut(s) 240, 604, 832
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 7 cut(s) 11, 322, 402, 436, 814, 1290, 1457
MluI ACGCGT 1 cut(s) 1002
MluNI TGGCCA 1 cut(s) 980
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 1 cut(s) 980
Mph1103I ATGCAT 2 cut(s) 871, 1285
MscI TGGCCA 1 cut(s) 980
MseI TTAA 9 cut(s) 14, 50, 375, 408, 488, 872, 924, 1278, 1314
MslI CAYNNNNRTG 4 cut(s) 425, 458, 980, 1299
Msp20I TGGCCA 1 cut(s) 980
MspI CCGG 3 cut(s) 205, 234, 313
MspR9I CCNGG 5 cut(s) 206, 235, 313, 314, 932
MunI CAATTG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 1 cut(s) 932
MvnI CGCG 2 cut(s) 222, 1004
MwoI GCNNNNNNNGC 3 cut(s) 170, 299, 1256
NciI CCSGG 4 cut(s) 206, 235, 313, 314
NcoI CCATGG 1 cut(s) 420
NdeII GATC 4 cut(s) 838, 1102, 1320, 1486
NlaIV GGNNCC 2 cut(s) 484, 608
NmuCI GTSAC 1 cut(s) 1085
NsiI ATGCAT 2 cut(s) 871, 1285
NspI RCATGY 4 cut(s) 177, 883, 1083, 1287
PaeI GCATGC 2 cut(s) 1083, 1287
PagI TCATGA 1 cut(s) 1227
PciI ACATGT 1 cut(s) 879
PcsI WCGNNNNNNNCGW 1 cut(s) 356
PctI GAATGC 1 cut(s) 562
PfeI GAWTC 1 cut(s) 1039
PkrI GCNGC 3 cut(s) 432, 1011, 1465
Ppu21I YACGTR 1 cut(s) 283
PscI ACATGT 1 cut(s) 879
PshBI ATTAAT 1 cut(s) 872
PsiI TTATAA 1 cut(s) 1501
Psp6I CCWGG 1 cut(s) 930
PspGI CCWGG 1 cut(s) 930
PspN4I GGNNCC 2 cut(s) 484, 608
PspPI GGNCC 1 cut(s) 202
PsuI RGATCY 1 cut(s) 838
RsaI GTAC 2 cut(s) 608, 961
RsaNI GTAC 2 cut(s) 607, 960
RseI CAYNNNNRTG 4 cut(s) 425, 458, 980, 1299
SaqAI TTAA 9 cut(s) 14, 50, 375, 408, 488, 872, 924, 1278, 1314
SatI GCNGC 3 cut(s) 431, 1010, 1464
Sau3AI GATC 4 cut(s) 838, 1102, 1320, 1486
Sau96I GGNCC 1 cut(s) 202
ScaI AGTACT 1 cut(s) 961
ScrFI CCNGG 5 cut(s) 206, 235, 313, 314, 932
SduI GDGCHC 3 cut(s) 240, 604, 832
SfaNI GCATC 2 cut(s) 311, 1365
SmaI CCCGGG 1 cut(s) 314
SmiMI CAYNNNNRTG 4 cut(s) 425, 458, 980, 1299
SmlI CTYRAG 2 cut(s) 167, 1337
SmoI CTYRAG 2 cut(s) 167, 1337
SnaBI TACGTA 1 cut(s) 283
SphI GCATGC 2 cut(s) 1083, 1287
Sse9I AATT 7 cut(s) 11, 322, 402, 436, 814, 1290, 1457
SsiI CCGC 5 cut(s) 89, 220, 431, 465, 1199
SspMI CTAG 4 cut(s) 651, 1028, 1097, 1178
StyD4I CCNGG 5 cut(s) 204, 233, 311, 312, 930
StyI CCWWGG 2 cut(s) 420, 1063
TaaI ACNGT 3 cut(s) 48, 606, 1404
TaiI ACGT 1 cut(s) 285
TaqI TCGA 7 cut(s) 359, 473, 782, 1101, 1195, 1379, 1485
TasI AATT 7 cut(s) 11, 322, 402, 436, 814, 1290, 1457
TatI WGTACW 1 cut(s) 959
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 1 cut(s) 1039
Tru1I TTAA 9 cut(s) 14, 50, 375, 408, 488, 872, 924, 1278, 1314
Tru9I TTAA 9 cut(s) 14, 50, 375, 408, 488, 872, 924, 1278, 1314
TscAI CASTG 3 cut(s) 567, 702, 812
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 2 cut(s) 1009, 1463
Tsp45I GTSAC 1 cut(s) 1085
TspMI CCCGGG 1 cut(s) 312
TspRI CASTG 3 cut(s) 567, 702, 812
VneI GTGCAC 2 cut(s) 600, 828
VspI ATTAAT 1 cut(s) 872
XapI RAATTY 2 cut(s) 814, 1290
XceI RCATGY 4 cut(s) 177, 883, 1083, 1287
XmaI CCCGGG 1 cut(s) 312
XmiI GTMKAC 1 cut(s) 997
XspI CTAG 4 cut(s) 651, 1028, 1097, 1178
ZrmI AGTACT 1 cut(s) 961
Zsp2I ATGCAT 2 cut(s) 871, 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.