Rh4BG136400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001684
Physical Location & Seq
Forward (+)
24243318 .. 24243788
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG136400.1

Sequence Viewer

Length: 471 bp
ATGATTTTGGACAATGCAATGTTGGATTTCTATCTGAGATGTGGTTCTTTGGTGGCTGCAAGATACTTGTTTGTCATTATGCCCAAGAGAGATTTTGTTTCTTGGAGTAGCATGGTGGGTGGTTATGCACATAGAGGTTTATGTGAGGAGGCAATGAGACTTTTCCAACAACTGGTGCTGGGCTGGGAAGTTGAGCCGAATGAGGCTACCATTGTCAATGTATTGTCAGCATGTTCTTCTGTTTGTTCCTTGAGTTCAAGCCAGCAGGTGCATTTCTATATCAGTACTCAGCCAGATCTCATGGTGAATAGCAATGTGGGAAATGCCTTGATCAACATGTATGTTAGGAGTGGACATTTGGGTATGGCTATTTCGGTTTTTAAGGCCTTGGAGTGCAAAGATATCATTTTATGGAGTACCATAATAAGCGGTACGGCATACATGTGTTGCAGCTCTTTTCCTGAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

156

Amino Acids

17.32

Weight (kDa)

5.49

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 33 - 58 5.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 172
AciI CCGC 1 cut(s) 429
AfaI GTAC 3 cut(s) 286, 418, 433
AfiI CCNNNNNNNGG 1 cut(s) 172
AflIII ACRYGT 2 cut(s) 336, 441
AgsI TTSAA 1 cut(s) 258
AluBI AGCT 1 cut(s) 453
AluI AGCT 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 151
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 2 cut(s) 56, 450
AsuHPI GGTGA 1 cut(s) 316
BbvI GCAGC 2 cut(s) 43, 462
BceAI ACGGC 1 cut(s) 450
BclI TGATCA 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 1 cut(s) 469
BfuAI ACCTGC 1 cut(s) 256
BglII AGATCT 1 cut(s) 295
BisI GCNGC 2 cut(s) 57, 451
BlsI GCNGC 2 cut(s) 58, 452
BmcAI AGTACT 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 271
BsaBI GATNNNNATC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 3 cut(s) 24, 159, 319
Bse8I GATNNNNATC 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 387
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMI GCAATG 3 cut(s) 24, 159, 319
BseMII CTCAG 2 cut(s) 26, 302
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 2 cut(s) 43, 462
BseYI CCCAGC 2 cut(s) 178, 183
BshFI GGCC 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 1 cut(s) 386
Bsp143I GATC 2 cut(s) 295, 330
BspACI CCGC 1 cut(s) 429
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 2 cut(s) 27, 301
BspMI ACCTGC 1 cut(s) 256
BsrDI GCAATG 3 cut(s) 24, 159, 319
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 295, 330
BssT1I CCWWGG 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 2 cut(s) 35, 288
BstKTI GATC 2 cut(s) 298, 333
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 2 cut(s) 295, 330
BstNSI RCATGY 3 cut(s) 234, 340, 445
BstV1I GCAGC 2 cut(s) 43, 462
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 1 cut(s) 386
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 263
Csp6I GTAC 3 cut(s) 285, 417, 432
CviAII CATG 5 cut(s) 112, 231, 301, 337, 442
CviJI RGCY 9 cut(s) 56, 183, 196, 206, 261, 292, 368, 386, 453
CviKI_1 RGCY 9 cut(s) 56, 183, 196, 206, 261, 292, 368, 386, 453
CviQI GTAC 3 cut(s) 285, 417, 432
DdeI CTNAG 2 cut(s) 35, 288
DpnI GATC 2 cut(s) 297, 332
DpnII GATC 2 cut(s) 295, 330
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 386
Eco32I GATATC 1 cut(s) 403
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 115, 234, 304, 340, 445
FatI CATG 5 cut(s) 111, 230, 300, 336, 441
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 2 cut(s) 57, 451
Fsp4HI GCNGC 2 cut(s) 57, 451
FspBI CTAG 1 cut(s) 469
GluI GCNGC 2 cut(s) 57, 451
GsaI CCCAGC 2 cut(s) 182, 187
HaeIII GGCC 1 cut(s) 386
Hin1II CATG 5 cut(s) 115, 234, 304, 340, 445
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 353
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 1 cut(s) 461
Hpy8I GTNNAC 1 cut(s) 353
HpyCH4V TGCA 6 cut(s) 17, 59, 128, 271, 396, 450
HpyF3I CTNAG 2 cut(s) 35, 288
Hsp92II CATG 5 cut(s) 115, 234, 304, 340, 445
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 2 cut(s) 295, 330
LpnPI CCDG 6 cut(s) 158, 164, 169, 251, 275, 306
Lsp1109I GCAGC 2 cut(s) 43, 462
MaeI CTAG 1 cut(s) 469
MalI GATC 2 cut(s) 297, 332
MboI GATC 2 cut(s) 295, 330
MboII GAAGA 1 cut(s) 228
MflI RGATCY 1 cut(s) 295
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 4 cut(s) 128, 139, 142, 196
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 470
NdeII GATC 2 cut(s) 295, 330
NlaIII CATG 5 cut(s) 115, 234, 304, 340, 445
NspI RCATGY 3 cut(s) 234, 340, 445
PaqCI CACCTGC 1 cut(s) 256
PceI AGGCCT 1 cut(s) 386
PciI ACATGT 2 cut(s) 336, 441
PctI GAATGC 1 cut(s) 470
PflMI CCANNNNNTGG 1 cut(s) 172
PkrI GCNGC 2 cut(s) 58, 452
PscI ACATGT 2 cut(s) 336, 441
PspFI CCCAGC 2 cut(s) 178, 183
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 3 cut(s) 286, 418, 433
RsaNI GTAC 3 cut(s) 285, 417, 432
RseI CAYNNNNRTG 1 cut(s) 442
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 57, 451
Sau3AI GATC 2 cut(s) 295, 330
ScaI AGTACT 1 cut(s) 286
SetI ASST 3 cut(s) 139, 270, 455
SmiMI CAYNNNNRTG 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 1 cut(s) 429
SspMI CTAG 1 cut(s) 469
StuI AGGCCT 1 cut(s) 386
StyI CCWWGG 1 cut(s) 387
TatI WGTACW 1 cut(s) 284
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 2 cut(s) 56, 450
Van91I CCANNNNNTGG 1 cut(s) 172
XceI RCATGY 3 cut(s) 234, 340, 445
XspI CTAG 1 cut(s) 469
ZrmI AGTACT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.