MD00G1175900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001684
Physical Location & Seq
Reverse (-)
40717130 .. 40717738
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1175900.v1.1.491

Sequence Viewer

Length: 609 bp
ATGATTTCGGGTCTTAACAAGTGTAGATTTGTGGAGGAAGCAATTGTTAAGTTTATGTCCATGGATGTGCCGCCTAATTCTACTACTCTTGTCATTGTCATGTTCGCTTGTTCGAGTTTAGGAGCTTTAAAGTTTGGTAAATCCGTTCATGGATATTGTATGAGGAAGTTATTTGAAAGGAATGTTATTTTGGACAATGCAGTGTTGGATTTTTACATGAGATGTGGGTCTTTGGTGAGTGCACGGTGCTTGTTTGTAAATATGCCCAAGAGAGATGTGTATTCTTGGACTAGCATGGTGGGTGGTTATGCACAAAAAGGATTTTGTGACGAGGCAGTGAAGCTTTTTCAGGAGATGGTTCAGGGAGAGGAAGTTGAGCCTAATGAAGATACCATTGTAAATGTATTGTCAGCGTGTTCTTCGATTGGGGCATTGAGTTTAGGCCAGTGGGTGAATTCGTATATAAGTGCACGGCCAGATCTCGTGTTGAATGGCATTCTGGGAAATGCATTAATAAACATGTATGTAAAGTATGGGCAAGTATGTATGGCAGTTTCGGTTTTTAAAACCCTGGTTTGCAAGGATCACATTTCGTGGAGTCCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

203

Amino Acids

22.35

Weight (kDa)

7.48

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 90 - 120 5.9e-06 PPR repeat
PPR_2 PF13041 91 - 139 1.1e-07 PPR repeat family
PPR PF01535 94 - 120 2.8e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 71
AclWI GGATC 1 cut(s) 591
AcoI YGGCCR 1 cut(s) 473
AcsI RAATTY 1 cut(s) 454
AflIII ACRYGT 1 cut(s) 519
AgsI TTSAA 2 cut(s) 176, 490
AjnI CCWGG 1 cut(s) 570
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 125, 343
AluI AGCT 2 cut(s) 125, 343
Alw21I GWGCWC 2 cut(s) 244, 472
Alw44I GTGCAC 2 cut(s) 240, 468
AlwI GGATC 1 cut(s) 591
AoxI GGCC 2 cut(s) 442, 473
ApaLI GTGCAC 2 cut(s) 240, 468
ApoI RAATTY 1 cut(s) 454
AseI ATTAAT 1 cut(s) 512
AsuHPI GGTGA 2 cut(s) 247, 463
BaeGI GKGCMC 2 cut(s) 244, 472
BaeI ACNNNNGTAYC 2 cut(s) 381, 414
BauI CACGAG 1 cut(s) 482
Bbv12I GWGCWC 2 cut(s) 244, 472
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 488
BciT130I CCWGG 1 cut(s) 572
BfaI CTAG 1 cut(s) 291
BglII AGATCT 1 cut(s) 478
BisI GCNGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 72
Bme1390I CCNGG 1 cut(s) 572
BmrFI CCNGG 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 60, 570
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 2 cut(s) 60, 570
BseGI GGATG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 445
BseSI GKGCMC 2 cut(s) 244, 472
BshFI GGCC 2 cut(s) 444, 475
BsiHKAI GWGCWC 2 cut(s) 244, 472
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 2 cut(s) 444, 475
Bsp1286I GDGCHC 2 cut(s) 244, 472
Bsp143I GATC 2 cut(s) 478, 583
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 444, 475
BspPI GGATC 1 cut(s) 591
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 60, 570
BssMI GATC 2 cut(s) 478, 583
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 1 cut(s) 246
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 1 cut(s) 70
BstKTI GATC 2 cut(s) 481, 586
BstMBI GATC 2 cut(s) 478, 583
BstNI CCWGG 1 cut(s) 572
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 570
BstSLI GKGCMC 2 cut(s) 244, 472
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 2 cut(s) 444, 475
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 70
BtsI GCAGTG 2 cut(s) 207, 342
BtsIMutI CAGTG 3 cut(s) 207, 342, 452
CviAII CATG 6 cut(s) 61, 100, 149, 217, 295, 520
CviJI RGCY 5 cut(s) 125, 343, 379, 444, 475
CviKI_1 RGCY 5 cut(s) 125, 343, 379, 444, 475
DpnI GATC 2 cut(s) 480, 585
DpnII GATC 2 cut(s) 478, 583
DraI TTTAAA 2 cut(s) 129, 565
EaeI YGGCCR 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 6 cut(s) 64, 103, 152, 220, 298, 523
FatI CATG 6 cut(s) 60, 99, 148, 216, 294, 519
Fnu4HI GCNGC 1 cut(s) 71
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 71
FspBI CTAG 1 cut(s) 291
GluI GCNGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 444, 475
Hin1II CATG 6 cut(s) 64, 103, 152, 220, 298, 523
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 1 cut(s) 598
HphI GGTGA 2 cut(s) 247, 463
Hpy166II GTNNAC 2 cut(s) 242, 470
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 2 cut(s) 242, 470
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4V TGCA 6 cut(s) 200, 242, 311, 470, 509, 579
Hsp92II CATG 6 cut(s) 64, 103, 152, 220, 298, 523
Kzo9I GATC 2 cut(s) 478, 583
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 7 cut(s) 335, 347, 458, 485, 489, 557, 584
MaeI CTAG 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 326
MalI GATC 2 cut(s) 480, 585
MboI GATC 2 cut(s) 478, 583
MboII GAAGA 2 cut(s) 398, 411
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 2 cut(s) 244, 472
MluCI AATT 3 cut(s) 42, 76, 454
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 4 cut(s) 28, 156, 325, 361
Mph1103I ATGCAT 1 cut(s) 511
MseI TTAA 5 cut(s) 15, 48, 128, 512, 564
MslI CAYNNNNRTG 2 cut(s) 65, 98
MspR9I CCNGG 1 cut(s) 572
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 1 cut(s) 572
NcoI CCATGG 1 cut(s) 60
NdeII GATC 2 cut(s) 478, 583
NlaIII CATG 6 cut(s) 64, 103, 152, 220, 298, 523
NmuCI GTSAC 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 523
PciI ACATGT 1 cut(s) 519
PctI GAATGC 1 cut(s) 495
PkrI GCNGC 1 cut(s) 72
PleI GAGTC 1 cut(s) 606
PpsI GAGTC 1 cut(s) 606
PscI ACATGT 1 cut(s) 519
PshBI ATTAAT 1 cut(s) 512
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PsuI RGATCY 1 cut(s) 478
RseI CAYNNNNRTG 2 cut(s) 65, 98
SaqAI TTAA 5 cut(s) 15, 48, 128, 512, 564
SatI GCNGC 1 cut(s) 71
Sau3AI GATC 2 cut(s) 478, 583
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 1 cut(s) 572
SduI GDGCHC 2 cut(s) 244, 472
SetI ASST 2 cut(s) 127, 345
SmiMI CAYNNNNRTG 2 cut(s) 65, 98
Sse9I AATT 3 cut(s) 42, 76, 454
SsiI CCGC 1 cut(s) 71
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 570
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 113, 422
TasI AATT 3 cut(s) 42, 76, 454
TauI GCSGC 1 cut(s) 73
Tru1I TTAA 5 cut(s) 15, 48, 128, 512, 564
Tru9I TTAA 5 cut(s) 15, 48, 128, 512, 564
TscAI CASTG 3 cut(s) 207, 342, 452
TseFI GTSAC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 2 cut(s) 137, 399
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 3 cut(s) 207, 342, 452
VneI GTGCAC 2 cut(s) 240, 468
VspI ATTAAT 1 cut(s) 512
XapI RAATTY 1 cut(s) 454
XceI RCATGY 1 cut(s) 523
XspI CTAG 1 cut(s) 291
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.