MD03G1186500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001684
Physical Location & Seq
Reverse (-)
25466879 .. 25468630
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1186500.v1.1.491

Sequence Viewer

Length: 1752 bp
ATGACTTTTGATATTTTTTTAATTTATTTGGAACAGCCGCCCTATACCCACCCCAAACCGCACGTACCAACCCCGACCGGCGGATCCCCCTCCTTCCTCCGCATCCCTTCCTCTGTGCAACCCAAATTCCACAATTGTTTCAGATGTCCGATTTCTCCTCAAGAAAAAGATGAATGCCCAGCAATTCCGACACCTACTGTGTATACATTTTCGTCAAAACATACAATGAAACGACAATTAAAGCAAATCATCCTTGCCCAAATCATCAAAAACCCTAAACCCCAACTCTTAAACCCTCTACTGGGACATCTCACAAACTCCCCCGCCCCACAAACTGCCCTTTTCCTCTACAACCAAATGCTCCACCACCCAACCTCCCACAACCACTACACCTTCACTTACGCTCTCAAGGCATGTTGCTTCCTCCATGATGACAACAAGGGCCGGGAAGTCCACGCCCGAGTCGTAAAAACCGGGCACTTTTCCGATACCTTCATCCAAAACTCGTTGCTTCACTTCTACGTAACCCAAGCTGACATTGTATCTGCTACCCGGGTTTTCGATTCCATACCAAACCCTGATGTTGTTTCGTGGACTTCGATGATTTCGGGTCTTAACAAGTGTGGATTTGTGGAGGAAGCAATTGTTAAGTTTATGTCCATGGATGTGCCGCCTAATTCTACTACTCTTGTCATTGTCATGTCCGCTTGTTCGAGTTTAGGAGCTTTAAAGTTTGGTAAATCTGTTCATGGATATTGTATGAGGAAGTTATCTGAAAGGAATGTTATTTTGGACAATGCAGTGTTGGATTTTTACATGAGATGTGGGTCTTTGGTGAGTGCACGGTACCTGTTTGTAAATATGCCCAGGAGAGATGTGTATTCGTGGACTAGCATGGTGGGTGGTTATGCACAAAGAGGATTTTGCGATGAGGCAGTGAAGCTTTTTCAGGGGATGGTTCAGGGAGAGGAAGTTGAGCCTAATGAGGCTACCATTGTAAATGTATTGGCAGCGTGTTCTTCGATTGGGGCATTGAGTTTAGGCCAGTGGGTGAATTCGTATATAAGTGCACGCCCAGATCTCGTGTTGAATGGCATTCTGGGAAATGCATTAATAAACATGTATGTAAAGTGTGGTGAAGTAGGTATGGCAGTTTCGGTTTTTAAAACCCTGGTTTGCAAGGATCACATTTCGTGGAGTACCATCATAAGCGGCATGGCCATGAATGGGTATGGTATACACACGTTGCAGCTCTTTTCGCTCATGCTAGTCCACGGGATTCCTCCTGATGATGTAACCTTCCTTGGCTTGTTATCAGCATGCAGTCACGCAGGTCTAGTCGATCAAGGGTTGATGATTTTCAATGCTATGAAAAATGTTTATCGGATTGCCCCTCAAACACTGCACTACGCCTGCCTAGTTGATATGTATGGTCGAGCGGGGTTCTTAGAGGAAGCAGAGAATTTCATGAAGGAAATGCCAACAGAAGCAGACGGCGTTGTTTGGGGAGCGTTGCTTAATGCTTGCAAAATTCATGGGAATGAGAAGATGCTTAAGAGGATCAGAAATGGTCTCCTCAAGAGTAGAGGAGTAAGCATCGGAACTTATGCTCTCATATCGAATACATACGCCACTCACGAACGGTGGGATGAGGCTAATAAGGTTCGTGATGAAATGAGACAAATGGGGTTGAAGAAATTAGCAGCGTACAGTCAGATAGATGTGGATCCAAACACATTAACGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

584

Amino Acids

64.89

Weight (kDa)

8.36

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 294 - 320 4.9e-06 PPR repeat
PPR_2 PF13041 395 - 442 3e-06 PPR repeat family
E_motif PF20431 534 - 571 7.5e-07 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1322
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 1 cut(s) 846
AccBSI CCGCTC 1 cut(s) 1439
AccI GTMKAC 2 cut(s) 203, 1237
AciI CCGC 9 cut(s) 38, 59, 81, 100, 324, 671, 705, 1212, 1439
AclI AACGTT 1 cut(s) 1742
AclWI GGATC 6 cut(s) 78, 91, 1191, 1568, 1721, 1734
AcoI YGGCCR 1 cut(s) 1218
AcsI RAATTY 4 cut(s) 125, 1054, 1462, 1530
AfaI GTAC 4 cut(s) 66, 848, 1201, 1709
AfiI CCNNNNNNNGG 4 cut(s) 80, 301, 302, 1227
AflII CTTAAG 1 cut(s) 1553
AflIII ACRYGT 2 cut(s) 1119, 1242
AgsI TTSAA 3 cut(s) 1090, 1363, 1693
AjnI CCWGG 2 cut(s) 866, 1170
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
AluBI AGCT 4 cut(s) 533, 725, 943, 1252
AluI AGCT 4 cut(s) 533, 725, 943, 1252
Alw21I GWGCWC 2 cut(s) 844, 1072
Alw26I GTCTC 2 cut(s) 1577, 1672
Alw44I GTGCAC 2 cut(s) 840, 1068
AlwI GGATC 6 cut(s) 78, 91, 1191, 1568, 1721, 1734
Ama87I CYCGRG 2 cut(s) 459, 552
AoxI GGCC 3 cut(s) 442, 1042, 1218
ApaLI GTGCAC 2 cut(s) 840, 1068
ApeKI GCWGC 3 cut(s) 1010, 1249, 1703
ApoI RAATTY 4 cut(s) 125, 1054, 1462, 1530
AseI ATTAAT 1 cut(s) 1112
Asp718I GGTACC 1 cut(s) 846
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 4 cut(s) 446, 475, 553, 554
AsuHPI GGTGA 3 cut(s) 847, 1063, 1148
AvaI CYCGRG 2 cut(s) 459, 552
BaeGI GKGCMC 3 cut(s) 480, 844, 1072
BalI TGGCCA 1 cut(s) 1220
BamHI GGATCC 2 cut(s) 83, 1726
BanI GGYRCC 1 cut(s) 846
BauI CACGAG 1 cut(s) 1082
Bbv12I GWGCWC 2 cut(s) 844, 1072
BbvI GCAGC 3 cut(s) 1022, 1261, 1715
BccI CCATC 2 cut(s) 949, 1211
BceAI ACGGC 1 cut(s) 1510
BciT130I CCWGG 2 cut(s) 868, 1172
BcnI CCSGG 4 cut(s) 446, 475, 553, 554
BcoDI GTCTC 2 cut(s) 1577, 1672
BfaI CTAG 4 cut(s) 891, 1268, 1337, 1418
BfrI CTTAAG 1 cut(s) 1553
BfuAI ACCTGC 1 cut(s) 1322
BglII AGATCT 1 cut(s) 1078
BisI GCNGC 6 cut(s) 38, 671, 1011, 1213, 1250, 1704
BlsI GCNGC 6 cut(s) 39, 672, 1012, 1214, 1251, 1705
Bme1390I CCNGG 6 cut(s) 446, 475, 553, 554, 868, 1172
BmeT110I CYCGRG 2 cut(s) 459, 552
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 3 cut(s) 85, 848, 1728
BmrFI CCNGG 6 cut(s) 446, 475, 553, 554, 868, 1172
BmrI ACTGGG 1 cut(s) 311
BmsI GCATC 3 cut(s) 111, 1539, 1605
BmuI ACTGGG 1 cut(s) 311
BpuEI CTTGAG 3 cut(s) 144, 392, 1562
BpuMI CCSGG 4 cut(s) 446, 475, 553, 554
BsaAI YACGTR 2 cut(s) 64, 523
BsaBI GATNNNNATC 1 cut(s) 1725
BsaI GGTCTC 1 cut(s) 1577
BsaJI CCNNGG 6 cut(s) 552, 660, 866, 1170, 1273, 1303
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 957, 987
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 301, 302, 1227
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 306, 1045
Bse8I GATNNNNATC 1 cut(s) 1725
BseBI CCWGG 2 cut(s) 868, 1172
BseDI CCNNGG 6 cut(s) 552, 660, 866, 1170, 1273, 1303
BseGI GGATG 6 cut(s) 102, 249, 495, 670, 960, 1654
BseJI GATNNNNATC 1 cut(s) 1725
BseLI CCNNNNNNNGG 4 cut(s) 80, 301, 302, 1227
BseNI ACTGG 2 cut(s) 306, 1045
BseRI GAGGAG 3 cut(s) 147, 1565, 1602
BseSI GKGCMC 3 cut(s) 480, 844, 1072
BseXI GCAGC 3 cut(s) 1022, 1261, 1715
BseYI CCCAGC 1 cut(s) 178
BsgI GTGCAG 1 cut(s) 1388
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 444, 1044, 1220
BshNI GGYRCC 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 844, 1072
BsiHKCI CYCGRG 2 cut(s) 459, 552
BsiSI CCGG 4 cut(s) 78, 445, 474, 553
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 4 cut(s) 80, 301, 302, 1227
BsmAI GTCTC 2 cut(s) 1577, 1672
BsmFI GGGAC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 179, 1095
BsnI GGCC 3 cut(s) 444, 1044, 1220
Bso31I GGTCTC 1 cut(s) 1577
BsoBI CYCGRG 2 cut(s) 459, 552
Bsp1286I GDGCHC 3 cut(s) 480, 844, 1072
Bsp143I GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
Bsp19I CCATGG 1 cut(s) 660
BspACI CCGC 9 cut(s) 38, 59, 81, 100, 324, 671, 705, 1212, 1439
BspANI GGCC 3 cut(s) 444, 1044, 1220
BspHI TCATGA 1 cut(s) 1467
BspLI GGNNCC 3 cut(s) 85, 848, 1728
BspMI ACCTGC 1 cut(s) 1322
BspPI GGATC 6 cut(s) 78, 91, 1191, 1568, 1721, 1734
BspT107I GGYRCC 1 cut(s) 846
BspTI CTTAAG 1 cut(s) 1553
BspTNI GGTCTC 1 cut(s) 1577
BsrBI CCGCTC 1 cut(s) 1439
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 2 cut(s) 306, 1045
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 6 cut(s) 552, 660, 866, 1170, 1273, 1303
BssMI GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
BssNAI GTATAC 2 cut(s) 204, 1238
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 2 cut(s) 660, 1303
Bst1107I GTATAC 2 cut(s) 204, 1238
Bst2BI CACGAG 1 cut(s) 1082
Bst2UI CCWGG 2 cut(s) 868, 1172
Bst4CI ACNGT 4 cut(s) 199, 846, 1644, 1712
BstAFI CTTAAG 1 cut(s) 1553
BstBAI YACGTR 2 cut(s) 64, 523
BstC8I GCNNGC 4 cut(s) 1072, 1321, 1414, 1525
BstDEI CTNAG 1 cut(s) 1447
BstDSI CCRYGG 2 cut(s) 660, 1273
BstF5I GGATG 6 cut(s) 102, 249, 495, 670, 960, 1654
BstKTI GATC 6 cut(s) 86, 1081, 1186, 1345, 1563, 1729
BstMAI GTCTC 2 cut(s) 1577, 1672
BstMBI GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 2 cut(s) 410, 1258
BstNI CCWGG 2 cut(s) 868, 1172
BstNSI RCATGY 3 cut(s) 417, 1123, 1323
BstSCI CCNGG 6 cut(s) 444, 473, 551, 552, 866, 1170
BstSLI GKGCMC 3 cut(s) 480, 844, 1072
BstSNI TACGTA 1 cut(s) 523
BstV1I GCAGC 3 cut(s) 1022, 1261, 1715
BstX2I RGATCY 3 cut(s) 83, 1078, 1726
BstYI RGATCY 3 cut(s) 83, 1078, 1726
BstZ17I GTATAC 2 cut(s) 204, 1238
BsuRI GGCC 3 cut(s) 444, 1044, 1220
BtgI CCRYGG 2 cut(s) 660, 1273
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 6 cut(s) 102, 249, 495, 670, 960, 1654
BtsI GCAGTG 3 cut(s) 807, 942, 1400
BtsIMutI CAGTG 4 cut(s) 807, 942, 1052, 1400
BveI ACCTGC 1 cut(s) 1322
Cac8I GCNNGC 4 cut(s) 1072, 1321, 1414, 1525
CciI TCATGA 1 cut(s) 1467
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 442
Cfr9I CCCGGG 1 cut(s) 552
Csp6I GTAC 4 cut(s) 65, 847, 1200, 1708
CviQI GTAC 4 cut(s) 65, 847, 1200, 1708
DdeI CTNAG 1 cut(s) 1447
DpnI GATC 6 cut(s) 85, 1080, 1185, 1344, 1562, 1728
DpnII GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
DraI TTTAAA 2 cut(s) 729, 1165
EaeI YGGCCR 1 cut(s) 1218
EciI GGCGGA 1 cut(s) 96
Eco105I TACGTA 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 660, 1303
Eco31I GGTCTC 1 cut(s) 1577
Eco88I CYCGRG 2 cut(s) 459, 552
EcoRI GAATTC 1 cut(s) 1054
EcoRII CCWGG 2 cut(s) 866, 1170
EcoT14I CCWWGG 2 cut(s) 660, 1303
EcoT22I ATGCAT 1 cut(s) 1111
ErhI CCWWGG 2 cut(s) 660, 1303
FaqI GGGAC 1 cut(s) 318
FauI CCCGC 2 cut(s) 331, 1432
FblI GTMKAC 2 cut(s) 203, 1237
Fnu4HI GCNGC 6 cut(s) 38, 671, 1011, 1213, 1250, 1704
FokI GGATG 6 cut(s) 89, 236, 482, 677, 967, 1661
Fsp4HI GCNGC 6 cut(s) 38, 671, 1011, 1213, 1250, 1704
FspBI CTAG 4 cut(s) 891, 1268, 1337, 1418
GluI GCNGC 6 cut(s) 38, 671, 1011, 1213, 1250, 1704
GsaI CCCAGC 1 cut(s) 182
HaeIII GGCC 3 cut(s) 444, 1044, 1220
HapII CCGG 4 cut(s) 78, 445, 474, 553
HindIII AAGCTT 1 cut(s) 941
HinfI GANTC 3 cut(s) 462, 563, 1279
HpaII CCGG 4 cut(s) 78, 445, 474, 553
HphI GGTGA 3 cut(s) 847, 1063, 1148
Hpy166II GTNNAC 8 cut(s) 204, 454, 594, 842, 888, 1070, 1238, 1273
Hpy188I TCNGA 9 cut(s) 143, 150, 189, 487, 775, 1386, 1565, 1601, 1716
Hpy188III TCNNGA 6 cut(s) 161, 1286, 1468, 1579, 1637, 1667
Hpy8I GTNNAC 8 cut(s) 204, 454, 594, 842, 888, 1070, 1238, 1273
HpyAV CCTTC 6 cut(s) 103, 117, 403, 502, 1309, 1465
HpyCH4III ACNGT 4 cut(s) 199, 846, 1644, 1712
HpyCH4IV ACGT 4 cut(s) 63, 522, 1244, 1742
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 1258
HpyF3I CTNAG 1 cut(s) 1447
HpySE526I ACGT 4 cut(s) 63, 522, 1244, 1742
KpnI GGTACC 1 cut(s) 850
Kzo9I GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
LmnI GCTCC 3 cut(s) 366, 722, 1508
Lsp1109I GCAGC 3 cut(s) 1022, 1261, 1715
LweI GCATC 3 cut(s) 111, 1539, 1605
MaeI CTAG 4 cut(s) 891, 1268, 1337, 1418
MaeII ACGT 4 cut(s) 63, 522, 1244, 1742
MaeIII GTNAC 3 cut(s) 523, 1294, 1325
MalI GATC 6 cut(s) 85, 1080, 1185, 1344, 1562, 1728
MbiI CCGCTC 1 cut(s) 1439
MboI GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
MboII GAAGA 3 cut(s) 1011, 1558, 1705
MfeI CAATTG 2 cut(s) 133, 642
MflI RGATCY 3 cut(s) 83, 1078, 1726
MhlI GDGCHC 3 cut(s) 480, 844, 1072
MlsI TGGCCA 1 cut(s) 1220
MluNI TGGCCA 1 cut(s) 1220
MlyI GAGTC 1 cut(s) 471
MmeI TCCRAC 2 cut(s) 212, 786
Mox20I TGGCCA 1 cut(s) 1220
Mph1103I ATGCAT 1 cut(s) 1111
MscI TGGCCA 1 cut(s) 1220
MslI CAYNNNNRTG 4 cut(s) 665, 698, 1220, 1539
Msp20I TGGCCA 1 cut(s) 1220
MspCI CTTAAG 1 cut(s) 1553
MspI CCGG 4 cut(s) 78, 445, 474, 553
MspR9I CCNGG 6 cut(s) 446, 475, 553, 554, 868, 1172
MunI CAATTG 2 cut(s) 133, 642
Mva1269I GAATGC 2 cut(s) 179, 1095
MvaI CCWGG 2 cut(s) 868, 1172
MwoI GCNNNNNNNGC 2 cut(s) 410, 1258
NciI CCSGG 4 cut(s) 446, 475, 553, 554
NcoI CCATGG 1 cut(s) 660
NdeII GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
NlaIV GGNNCC 3 cut(s) 85, 848, 1728
NmuCI GTSAC 1 cut(s) 1325
NsiI ATGCAT 1 cut(s) 1111
NspI RCATGY 3 cut(s) 417, 1123, 1323
PaeI GCATGC 1 cut(s) 1323
PagI TCATGA 1 cut(s) 1467
PciI ACATGT 1 cut(s) 1119
PcsI WCGNNNNNNNCGW 3 cut(s) 462, 596, 1635
PctI GAATGC 2 cut(s) 179, 1095
PfeI GAWTC 2 cut(s) 563, 1279
PkrI GCNGC 6 cut(s) 39, 672, 1012, 1214, 1251, 1705
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
Ppu21I YACGTR 2 cut(s) 64, 523
PscI ACATGT 1 cut(s) 1119
PshBI ATTAAT 1 cut(s) 1112
Psp1406I AACGTT 1 cut(s) 1742
Psp6I CCWGG 2 cut(s) 866, 1170
PspFI CCCAGC 1 cut(s) 178
PspGI CCWGG 2 cut(s) 866, 1170
PspN4I GGNNCC 3 cut(s) 85, 848, 1728
PspPI GGNCC 1 cut(s) 442
PsuI RGATCY 3 cut(s) 83, 1078, 1726
RsaI GTAC 4 cut(s) 66, 848, 1201, 1709
RsaNI GTAC 4 cut(s) 65, 847, 1200, 1708
RseI CAYNNNNRTG 4 cut(s) 665, 698, 1220, 1539
SatI GCNGC 6 cut(s) 38, 671, 1011, 1213, 1250, 1704
Sau3AI GATC 6 cut(s) 83, 1078, 1183, 1342, 1560, 1726
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 1 cut(s) 471
ScrFI CCNGG 6 cut(s) 446, 475, 553, 554, 868, 1172
SduI GDGCHC 3 cut(s) 480, 844, 1072
SfaNI GCATC 3 cut(s) 111, 1539, 1605
SmaI CCCGGG 1 cut(s) 554
SmiMI CAYNNNNRTG 4 cut(s) 665, 698, 1220, 1539
SmlI CTYRAG 4 cut(s) 159, 407, 1553, 1577
SmoI CTYRAG 4 cut(s) 159, 407, 1553, 1577
SnaBI TACGTA 1 cut(s) 523
SphI GCATGC 1 cut(s) 1323
SsiI CCGC 9 cut(s) 38, 59, 81, 100, 324, 671, 705, 1212, 1439
SspMI CTAG 4 cut(s) 891, 1268, 1337, 1418
StyD4I CCNGG 6 cut(s) 444, 473, 551, 552, 866, 1170
StyI CCWWGG 2 cut(s) 660, 1303
TaaI ACNGT 4 cut(s) 199, 846, 1644, 1712
TaiI ACGT 4 cut(s) 66, 525, 1247, 1745
TaqI TCGA 7 cut(s) 561, 599, 713, 1022, 1341, 1435, 1619
TauI GCSGC 3 cut(s) 40, 673, 1215
TfiI GAWTC 2 cut(s) 563, 1279
TscAI CASTG 4 cut(s) 807, 942, 1052, 1407
TseFI GTSAC 1 cut(s) 1325
TseI GCWGC 3 cut(s) 1010, 1249, 1703
Tsp45I GTSAC 1 cut(s) 1325
TspMI CCCGGG 1 cut(s) 552
TspRI CASTG 4 cut(s) 807, 942, 1052, 1407
Vha464I CTTAAG 1 cut(s) 1553
VneI GTGCAC 2 cut(s) 840, 1068
VspI ATTAAT 1 cut(s) 1112
XapI RAATTY 4 cut(s) 125, 1054, 1462, 1530
XceI RCATGY 3 cut(s) 417, 1123, 1323
XmaI CCCGGG 1 cut(s) 552
XmiI GTMKAC 2 cut(s) 203, 1237
XspI CTAG 4 cut(s) 891, 1268, 1337, 1418
Zsp2I ATGCAT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.