RchiOBHm_Chr7g0232221

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001684
Physical Location & Seq
Forward (+)
56174927 .. 56179432
4506 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20813

Sequence Viewer

Length: 1341 bp
ATGAGACAACTAAACCAAATCCATGCGCTGCTGGTCAAAAATCCAAGACCCCAAGTCTTAAACCCCTGGCTGGGATATCTGACAAAGTCTTCCGCACCACAAAATGCCCTTTTTCTCTATAACCAAATGCTCCACCACCCAACTTCCCATAACCACTACACATTTACCTATGCTCTAAAGGCATGCTGCTTGCTCCATTCACCCCATAAAGGCCAAGAAATCCAAGCCCACGTCACAAAATCCGGTCACATATCTGATACCTTCATCCAAAATTCTTTGCTCCACTTTTACGTAATCCAATCTGACATTGTTTCTGCTACCCGCGTTTTTGATTCAATACCCATACCGGATGTTGTTTCCTGGACTTCGATGATTTCGGGTCTTTCCAAGTGTGGGTTTGTGGAGGAAGCCATTGTCAAGTTTATGTCCATGGACGTGAAGCCTAATTCCACTACTCTTGTGACTGTTTTGTCTTCTTGTTCTACTTTAAGAGCTTTGAAATTTGGTAAAGCTTTTCATGGCCATTGTTTGAGGAACTTTCGGGAAAGAAATTTGATTTTGGACAATGCGGTGTTGGATTTCTATCTGAGATGTGGATCTTTGGCGAGTGCACGATACCTGTTTGTCAATATGCCCAAGAGAGATGTGGTTTCTTGGACTATCGTGGTGGGTGGTTATGCACAAAGAGGTTTTTGTGAAGAGGCAGTGAAACTTTTCCAACAACTGCTGCAGGGTGGAGAAGCTGAGCCTAATGAGGCTACCATTGTCAATGTATTGTCAGCATGTTCTTCGATTTGTGCATTGAGTTCAGGCCAGCAGGTGCATGCCTATATCAGTAATCGACGAGATCTCATGGCGAATGTCAATGTGGGGAATGCTTTAATCAATTTGTATGTGAAGTGTGGAAACTTGGAAATGGCTATCTCAGTTTTTCAGTCCCTGGGGCACAAAGCTATCATTTCATGGAGTACCATAATATGCGGCATGGCCATGAATGGCCATGGCATACACGTGTTGCAGCTCTTTTCCAGTATGCTAGTCAACGGGGTGTCTCCTGATGGTGCTTTTATTAAAGAAATGCCAACAGAAGCTGATGGTCCTGTTTGGGGAGCGCTGCTTAATGCTTGTAAAATCTATGGGAATGAGGAGATGGTTGAGAGGATCAGAGAACGCCTCCGCAATAGAGCAGGAGTGAGCACCGGAACTTATGCTCTGTTATCAAATGCTTATGCTAAACATGATAGGTGGGATGATTCTAATAAGGTTCGTGATGAAATGAGAGAGATGGGGTTGAAGGCACCACCAGGTCGTAGTTGGATTGAAATTGGTCCGTCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

446

Amino Acids

49.5

Weight (kDa)

8.75

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 116 - 160 8.6e-06 PPR repeat family
E_motif PF20431 379 - 441 1.8e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 808
AasI GACNNNNNNGTC 1 cut(s) 469
Acc36I ACCTGC 1 cut(s) 808
AccB1I GGYRCC 1 cut(s) 1297
AccII CGCG 1 cut(s) 324
AciI CCGC 5 cut(s) 93, 322, 569, 981, 1177
AclWI GGATC 2 cut(s) 604, 1169
AcoI YGGCCR 3 cut(s) 520, 987, 997
AcsI RAATTY 3 cut(s) 271, 500, 550
AcvI CACGTG 1 cut(s) 1012
AfaI GTAC 1 cut(s) 970
AfeI AGCGCT 1 cut(s) 1113
AfiI CCNNNNNNNGG 5 cut(s) 70, 71, 209, 393, 1106
AflIII ACRYGT 2 cut(s) 1009, 1011
AgsI TTSAA 4 cut(s) 336, 499, 1294, 1322
AhdI GACNNNNNGTC 1 cut(s) 53
AjiI CACGTC 2 cut(s) 232, 436
AjnI CCWGG 4 cut(s) 65, 359, 939, 1303
AluBI AGCT 6 cut(s) 494, 512, 743, 953, 1021, 1091
AluI AGCT 6 cut(s) 494, 512, 743, 953, 1021, 1091
Alw21I GWGCWC 2 cut(s) 613, 1199
Alw26I GTCTC 1 cut(s) 1056
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 2 cut(s) 604, 1169
AlwNI CAGNNNCTG 2 cut(s) 940, 1091
Aor51HI AGCGCT 1 cut(s) 1113
AoxI GGCC 5 cut(s) 211, 520, 811, 987, 997
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 5 cut(s) 28, 186, 727, 1018, 1114
ApoI RAATTY 3 cut(s) 271, 500, 550
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 2 cut(s) 28, 1114
AspS9I GGNCC 2 cut(s) 1097, 1328
AsuHPI GGTGA 1 cut(s) 192
AvaII GGWCC 2 cut(s) 1097, 1328
BaeGI GKGCMC 2 cut(s) 613, 948
BalI TGGCCA 3 cut(s) 522, 989, 999
BanI GGYRCC 1 cut(s) 1297
BbrPI CACGTG 1 cut(s) 1012
BbsI GAAGAC 2 cut(s) 81, 465
Bbv12I GWGCWC 2 cut(s) 613, 1199
BbvI GCAGC 5 cut(s) 15, 173, 714, 1030, 1101
BccI CCATC 4 cut(s) 1052, 1088, 1144, 1279
BcgI CGANNNNNNTGC 2 cut(s) 771, 805
BciT130I CCWGG 4 cut(s) 67, 361, 941, 1305
BcoDI GTCTC 1 cut(s) 1056
BfaI CTAG 1 cut(s) 1037
BfmI CTRYAG 1 cut(s) 728
BfoI RGCGCY 1 cut(s) 1115
BfuAI ACCTGC 1 cut(s) 808
BglII AGATCT 1 cut(s) 847
BisI GCNGC 6 cut(s) 29, 187, 728, 982, 1019, 1115
BlpI GCTNAGC 1 cut(s) 744
BlsI GCNGC 6 cut(s) 30, 188, 729, 983, 1020, 1116
Bme1390I CCNGG 4 cut(s) 67, 361, 941, 1305
Bme18I GGWCC 2 cut(s) 1097, 1328
BmeRI GACNNNNNGTC 1 cut(s) 53
BmgBI CACGTC 2 cut(s) 232, 436
BmgT120I GGNCC 2 cut(s) 1097, 1328
BmiI GGNNCC 1 cut(s) 1299
BmrFI CCNGG 4 cut(s) 67, 361, 941, 1305
BpiI GAAGAC 2 cut(s) 81, 465
BplI GAGNNNNNCTC 2 cut(s) 1158, 1190
Bpu1102I GCTNAGC 1 cut(s) 744
BsaAI YACGTR 2 cut(s) 292, 1012
BsaBI GATNNNNATC 2 cut(s) 582, 595
BsaJI CCNNGG 5 cut(s) 65, 429, 939, 940, 1000
BsaWI WCCGGW 3 cut(s) 242, 346, 1199
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 71, 209, 393, 1106
Bse1I ACTGG 1 cut(s) 1029
Bse8I GATNNNNATC 2 cut(s) 582, 595
BseBI CCWGG 4 cut(s) 67, 361, 941, 1305
BseDI CCNNGG 5 cut(s) 65, 429, 939, 940, 1000
BseGI GGATG 3 cut(s) 264, 355, 1255
BseJI GATNNNNATC 2 cut(s) 582, 595
BseLI CCNNNNNNNGG 5 cut(s) 70, 71, 209, 393, 1106
BseMII CTCAG 3 cut(s) 578, 735, 939
BseNI ACTGG 1 cut(s) 1029
BseRI GAGGAG 1 cut(s) 1160
BseSI GKGCMC 2 cut(s) 613, 948
BseXI GCAGC 5 cut(s) 15, 173, 714, 1030, 1101
BseYI CCCAGC 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 5 cut(s) 213, 522, 813, 989, 999
BshNI GGYRCC 1 cut(s) 1297
BsiHKAI GWGCWC 2 cut(s) 613, 1199
BsiSI CCGG 3 cut(s) 243, 347, 1200
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 5 cut(s) 70, 71, 209, 393, 1106
BsmAI GTCTC 1 cut(s) 1056
BsmFI GGGAC 1 cut(s) 922
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 5 cut(s) 213, 522, 813, 989, 999
Bsp1286I GDGCHC 3 cut(s) 613, 948, 1199
Bsp143I GATC 3 cut(s) 596, 847, 1161
Bsp1720I GCTNAGC 1 cut(s) 744
Bsp19I CCATGG 2 cut(s) 429, 1000
BspACI CCGC 5 cut(s) 93, 322, 569, 981, 1177
BspANI GGCC 5 cut(s) 213, 522, 813, 989, 999
BspCNI CTCAG 3 cut(s) 579, 736, 938
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 1299
BspMAI CTGCAG 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 2 cut(s) 604, 1169
BspT107I GGYRCC 1 cut(s) 1297
BsrI ACTGG 1 cut(s) 1029
BssECI CCNNGG 5 cut(s) 65, 429, 939, 940, 1000
BssMI GATC 3 cut(s) 596, 847, 1161
BssT1I CCWWGG 2 cut(s) 429, 1000
Bst2UI CCWGG 4 cut(s) 67, 361, 941, 1305
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 693
BstBAI YACGTR 2 cut(s) 292, 1012
BstC8I GCNNGC 4 cut(s) 184, 191, 815, 825
BstDEI CTNAG 3 cut(s) 587, 744, 925
BstDSI CCRYGG 2 cut(s) 429, 1000
BstF5I GGATG 3 cut(s) 264, 355, 1255
BstFNI CGCG 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 1115
BstHHI GCGC 2 cut(s) 28, 1114
BstKTI GATC 3 cut(s) 599, 850, 1164
BstMAI GTCTC 1 cut(s) 1056
BstMBI GATC 3 cut(s) 596, 847, 1161
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 4 cut(s) 67, 361, 941, 1305
BstNSI RCATGY 3 cut(s) 186, 786, 827
BstSCI CCNGG 4 cut(s) 65, 359, 939, 1303
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 2 cut(s) 613, 948
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 5 cut(s) 15, 173, 714, 1030, 1101
BstV2I GAAGAC 2 cut(s) 81, 465
BstX2I RGATCY 2 cut(s) 596, 847
BstYI RGATCY 2 cut(s) 596, 847
BsuRI GGCC 5 cut(s) 213, 522, 813, 989, 999
BtgI CCRYGG 2 cut(s) 429, 1000
BtrI CACGTC 2 cut(s) 232, 436
BtsCI GGATG 3 cut(s) 264, 355, 1255
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 1 cut(s) 711
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 4 cut(s) 184, 191, 815, 825
CaiI CAGNNNCTG 2 cut(s) 940, 1091
CfoI GCGC 2 cut(s) 28, 1114
Cfr13I GGNCC 2 cut(s) 1097, 1328
CsiI ACCWGGT 1 cut(s) 1303
Csp6I GTAC 1 cut(s) 969
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 1 cut(s) 969
DdeI CTNAG 3 cut(s) 587, 744, 925
DpnI GATC 3 cut(s) 598, 849, 1163
DpnII GATC 3 cut(s) 596, 847, 1161
DrdI GACNNNNNNGTC 1 cut(s) 469
DriI GACNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 3 cut(s) 520, 987, 997
Eam1104I CTCTTC 1 cut(s) 693
Eam1105I GACNNNNNGTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 693
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 429, 1000
Eco32I GATATC 1 cut(s) 77
Eco47I GGWCC 2 cut(s) 1097, 1328
Eco47III AGCGCT 1 cut(s) 1113
Eco72I CACGTG 1 cut(s) 1012
EcoRII CCWGG 4 cut(s) 65, 359, 939, 1303
EcoRV GATATC 1 cut(s) 77
EcoT14I CCWWGG 2 cut(s) 429, 1000
ErhI CCWWGG 2 cut(s) 429, 1000
FaqI GGGAC 1 cut(s) 922
FauI CCCGC 1 cut(s) 329
Fnu4HI GCNGC 6 cut(s) 29, 187, 728, 982, 1019, 1115
FokI GGATG 3 cut(s) 251, 362, 1262
Fsp4HI GCNGC 6 cut(s) 29, 187, 728, 982, 1019, 1115
FspBI CTAG 1 cut(s) 1037
GlaI GCGC 2 cut(s) 27, 1113
GluI GCNGC 6 cut(s) 29, 187, 728, 982, 1019, 1115
GsaI CCCAGC 1 cut(s) 74
HaeII RGCGCY 1 cut(s) 1115
HaeIII GGCC 5 cut(s) 213, 522, 813, 989, 999
HapII CCGG 3 cut(s) 243, 347, 1200
HhaI GCGC 2 cut(s) 28, 1114
Hin6I GCGC 2 cut(s) 26, 1112
HinP1I GCGC 2 cut(s) 26, 1112
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HindIII AAGCTT 1 cut(s) 510
HinfI GANTC 2 cut(s) 332, 1253
HpaII CCGG 3 cut(s) 243, 347, 1200
HphI GGTGA 1 cut(s) 192
Hpy166II GTNNAC 2 cut(s) 611, 1042
Hpy188I TCNGA 5 cut(s) 81, 256, 304, 588, 1166
Hpy188III TCNNGA 3 cut(s) 542, 1055, 1268
Hpy8I GTNNAC 2 cut(s) 611, 1042
Hpy99I CGWCG 1 cut(s) 846
HpyAV CCTTC 2 cut(s) 271, 1288
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 4 cut(s) 231, 291, 435, 1011
HpyCH4V TGCA 6 cut(s) 611, 680, 730, 800, 823, 1018
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 3 cut(s) 587, 744, 925
HpySE526I ACGT 4 cut(s) 231, 291, 435, 1011
HspAI GCGC 2 cut(s) 26, 1112
Kzo9I GATC 3 cut(s) 596, 847, 1161
LmnI GCTCC 4 cut(s) 135, 198, 285, 1109
Lsp1109I GCAGC 5 cut(s) 15, 173, 714, 1030, 1101
MabI ACCWGGT 1 cut(s) 1303
MaeI CTAG 1 cut(s) 1037
MaeII ACGT 4 cut(s) 231, 291, 435, 1011
MaeIII GTNAC 3 cut(s) 232, 245, 460
MalI GATC 3 cut(s) 598, 849, 1163
MboI GATC 3 cut(s) 596, 847, 1161
MboII GAAGA 4 cut(s) 81, 465, 710, 780
MflI RGATCY 2 cut(s) 596, 847
MhlI GDGCHC 3 cut(s) 613, 948, 1199
MlsI TGGCCA 3 cut(s) 522, 989, 999
MluCI AATT 6 cut(s) 271, 445, 500, 550, 886, 1323
MluNI TGGCCA 3 cut(s) 522, 989, 999
MmeI TCCRAC 3 cut(s) 555, 742, 1295
MnlI CCTC 8 cut(s) 397, 525, 680, 694, 748, 1138, 1152, 1184
Mox20I TGGCCA 3 cut(s) 522, 989, 999
MroXI GAANNNNTTC 1 cut(s) 713
MscI TGGCCA 3 cut(s) 522, 989, 999
MseI TTAA 5 cut(s) 59, 488, 881, 1071, 1119
MslI CAYNNNNRTG 3 cut(s) 434, 989, 1010
Msp20I TGGCCA 3 cut(s) 522, 989, 999
MspI CCGG 3 cut(s) 243, 347, 1200
MspR9I CCNGG 4 cut(s) 67, 361, 941, 1305
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 4 cut(s) 67, 361, 941, 1305
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 179
NcoI CCATGG 2 cut(s) 429, 1000
NdeII GATC 3 cut(s) 596, 847, 1161
NlaIV GGNNCC 1 cut(s) 1299
NmuCI GTSAC 3 cut(s) 232, 245, 460
NspI RCATGY 3 cut(s) 186, 786, 827
PaeI GCATGC 2 cut(s) 186, 827
PaqCI CACCTGC 1 cut(s) 808
PasI CCCWGGG 1 cut(s) 940
PctI GAATGC 1 cut(s) 880
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 332, 1253
PflFI GACNNNGTC 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 6 cut(s) 30, 188, 729, 983, 1020, 1116
PmaCI CACGTG 1 cut(s) 1012
PmlI CACGTG 1 cut(s) 1012
Ppu21I YACGTR 2 cut(s) 292, 1012
Psp6I CCWGG 4 cut(s) 65, 359, 939, 1303
PspCI CACGTG 1 cut(s) 1012
PspFI CCCAGC 1 cut(s) 70
PspGI CCWGG 4 cut(s) 65, 359, 939, 1303
PspN4I GGNNCC 1 cut(s) 1299
PspPI GGNCC 2 cut(s) 1097, 1328
PstI CTGCAG 1 cut(s) 732
PstNI CAGNNNCTG 2 cut(s) 940, 1091
PsuI RGATCY 2 cut(s) 596, 847
PsyI GACNNNGTC 1 cut(s) 85
RsaI GTAC 1 cut(s) 970
RsaNI GTAC 1 cut(s) 969
RseI CAYNNNNRTG 3 cut(s) 434, 989, 1010
SaqAI TTAA 5 cut(s) 59, 488, 881, 1071, 1119
SatI GCNGC 6 cut(s) 29, 187, 728, 982, 1019, 1115
Sau3AI GATC 3 cut(s) 596, 847, 1161
Sau96I GGNCC 2 cut(s) 1097, 1328
ScrFI CCNGG 4 cut(s) 67, 361, 941, 1305
SduI GDGCHC 3 cut(s) 613, 948, 1199
SexAI ACCWGGT 1 cut(s) 1303
SfcI CTRYAG 1 cut(s) 728
SinI GGWCC 2 cut(s) 1097, 1328
SmiMI CAYNNNNRTG 3 cut(s) 434, 989, 1010
SnaBI TACGTA 1 cut(s) 292
SphI GCATGC 2 cut(s) 186, 827
Sse9I AATT 6 cut(s) 271, 445, 500, 550, 886, 1323
SsiI CCGC 5 cut(s) 93, 322, 569, 981, 1177
SspMI CTAG 1 cut(s) 1037
StyD4I CCNGG 4 cut(s) 65, 359, 939, 1303
StyI CCWWGG 2 cut(s) 429, 1000
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 4 cut(s) 234, 294, 438, 1014
TaqI TCGA 3 cut(s) 368, 791, 841
TasI AATT 6 cut(s) 271, 445, 500, 550, 886, 1323
TauI GCSGC 1 cut(s) 984
TfiI GAWTC 2 cut(s) 332, 1253
Tru1I TTAA 5 cut(s) 59, 488, 881, 1071, 1119
Tru9I TTAA 5 cut(s) 59, 488, 881, 1071, 1119
TscAI CASTG 1 cut(s) 711
TseFI GTSAC 3 cut(s) 232, 245, 460
TseI GCWGC 5 cut(s) 28, 186, 727, 1018, 1114
Tsp45I GTSAC 3 cut(s) 232, 245, 460
TspDTI ATGAA 5 cut(s) 253, 506, 951, 1007, 1287
TspGWI ACGGA 1 cut(s) 1320
TspRI CASTG 1 cut(s) 711
Tth111I GACNNNGTC 1 cut(s) 85
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 2 cut(s) 1097, 1328
XapI RAATTY 3 cut(s) 271, 500, 550
XceI RCATGY 3 cut(s) 186, 786, 827
XcmI CCANNNNNNNNNTGG 2 cut(s) 643, 1311
XmnI GAANNNNTTC 1 cut(s) 713
XspI CTAG 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.