pycom03g13340

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001684
Physical Location & Seq
Forward (+)
14309996 .. 14311733
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13340.1

Sequence Viewer

Length: 1686 bp
ATGGAGGACGTTCCCCCTCCTTCCTCCGCCTTCCTTCCTCTGCGCAACCCAAATTCCCACCCAACCGCTGCCCACAATTGTTTCAGATGTCCGATTTCTCCTCGAGAAAAAGATAAATGGCCAGCAATTCCGAAACCTACTGTTTATACATTTTCGTTGAAAAGTACAATGAAACGTCAATTAAAGCAAATCATCCTTGCCCAAATCATCAAAAACCCTAAACCCCAACTCTTAAACCCTCTACTGGGACATCTCACAAACTCTCCCGCCCCACAAACTGCCCTTTTCCTCTACAACCAAATGCTCCACTTCCCAACCTCCCACAATCACTACACCTTCACTTACGCTCTCAAGGCATGTTGCTTCCTCCATGATGACAACAAGGGACGGGAAGTCCACGCCCGCGTCGTAAAAACTGGGCACTTTTCCGATACCTTCATCCAAAACTCGTTGCTTCATTTCTACGTAACCCAAGCTGACATTGTATCCGCTACCCGGGTTTTCGAATCCATACCAAACCCCGATGTTGTTTCGTGGACTTCGATGATTTCGGGTCTTAACAAGTGTGGATTTGTGGAGGAAGCAATTGTTAAGTTTATGTCCATGGATGTGCAGCCTAATTCTACTACTCTTGTCATTGTTATGTCCGCTTGTTCGAGTTTAGGAGCTTTAAAGTTTGGTAAATCTGTTCATGGATATTGTATGAGGAAGTTATCTGAAAGGAATGTTATTTGGGACAATGCAGTGTTGGATTTTTACATGAGATGTGGGTCTTTGGTGAGTGGACGGTACCTGTTTGTAAATATGCCAAAGAGAGATGTGTATTCTTGGACTAGCATGGTGGGTGGTTATGCACAAAGAGGATTTTGTGATGAGGCAGTGAGGCTTTTTCAGGAGATGGTTCAGGGAGAGGAAGTTGAGCCTAATGAGGCTACCATTGTAAATGTATTGTCAGCGTGTTCTTCGATTGGAGCATTGAGTTTAGGCCAGTGGGTGAATTCGTATATAAGTGCGCGGCCAGATCTCATGTTGAATGGCATTGTGGGAAATGCATTAATAAACATGTATGTAAAGTGTGGTGAAGTAGGTATGGCAGTTTCAGTTTTTAAAACCCTGGTTTGCAAGAATAACGTTTCGTGGAGTACCATCATAAGCGGCATGGCCATGAATGGTTATGGTATACATGCGTTGCAGCTCTTTTCGCTCATGCTAGTCCACGGGATTCCTCCTGATGATGTAACCTTCCTTGGCGTGTTATCAGCATGCAGTCACGCAGGTCTAGTCGATCGAGGGTCAATGTTTTTCAATGCTATGAAAAATGTTTATCAGATCGCCCCTCAAACACAGCATTACGTCTGCCTAGTTGATATGTATGGTCGAGCGGGGTTCTTGGAGGAAGCAGAGAGTTTCATTAAGGAAATGCCAACAGAAGCAGACGGCGCTGTTTGGGGAGCGTTTCTTAATGCATGCAAAATTCATGGGAATGAGAAGATGTTTGAGAGGATCAGAAATGATCTCCTCAAGAGTAGAGGAGTAAGCATCGGAACTTATGCTCTTGTATCGAATACATACGCTACTCACGAACGGTGGGATGAGGCTAATAAGGTTCGTGATGAAATGAGACAGAAGGGGTTGAAGAAATTAGCAGCATATAGCCAGATAGATGTTGATCCAAATATCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

562

Amino Acids

62.53

Weight (kDa)

8.39

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 272 - 320 1.1e-06 PPR repeat family
PPR PF01535 275 - 301 4.1e-07 PPR repeat
PPR_2 PF13041 376 - 423 1.3e-07 PPR repeat family
E_motif PF20431 515 - 552 3.1e-07 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1684
Acc16I TGCGCA 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 1265
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 789
AccBSI CCGCTC 1 cut(s) 1382
AccI GTMKAC 1 cut(s) 1180
AccII CGCG 2 cut(s) 405, 1015
AciI CCGC 9 cut(s) 27, 66, 267, 403, 489, 648, 1015, 1155, 1382
AclI AACGTT 1 cut(s) 1131
AclWI GGATC 2 cut(s) 1511, 1664
AcoI YGGCCR 3 cut(s) 119, 1016, 1161
AcsI RAATTY 3 cut(s) 52, 997, 1473
AfaI GTAC 3 cut(s) 166, 791, 1144
AfiI CCNNNNNNNGG 3 cut(s) 244, 245, 495
AflIII ACRYGT 1 cut(s) 1062
AgsI TTSAA 4 cut(s) 160, 1033, 1306, 1636
AhdI GACNNNNNGTC 1 cut(s) 392
AjnI CCWGG 1 cut(s) 1113
AluBI AGCT 3 cut(s) 476, 668, 1195
AluI AGCT 3 cut(s) 476, 668, 1195
Alw26I GTCTC 1 cut(s) 1615
AlwI GGATC 2 cut(s) 1511, 1664
Ama87I CYCGRG 2 cut(s) 102, 495
AoxI GGCC 4 cut(s) 119, 985, 1016, 1161
ApeKI GCWGC 4 cut(s) 68, 613, 1192, 1646
ApoI RAATTY 3 cut(s) 52, 997, 1473
AseI ATTAAT 1 cut(s) 1055
Asp718I GGTACC 1 cut(s) 789
AspLEI GCGC 3 cut(s) 45, 1015, 1442
AsuC2I CCSGG 2 cut(s) 496, 497
AsuHPI GGTGA 3 cut(s) 790, 1006, 1091
AsuII TTCGAA 1 cut(s) 504
AvaI CYCGRG 2 cut(s) 102, 495
BaeGI GKGCMC 1 cut(s) 423
BalI TGGCCA 2 cut(s) 121, 1163
BanI GGYRCC 1 cut(s) 789
BbvI GCAGC 4 cut(s) 55, 625, 1204, 1658
BccI CCATC 2 cut(s) 892, 1154
BceAI ACGGC 1 cut(s) 1453
BciT130I CCWGG 1 cut(s) 1115
BciVI GTATCC 1 cut(s) 496
BcnI CCSGG 2 cut(s) 496, 497
BcoDI GTCTC 1 cut(s) 1615
BfaI CTAG 4 cut(s) 834, 1211, 1280, 1361
BfoI RGCGCY 1 cut(s) 1443
BfuAI ACCTGC 1 cut(s) 1265
BfuI GTATCC 1 cut(s) 496
BglII AGATCT 1 cut(s) 1021
BisI GCNGC 6 cut(s) 69, 614, 1016, 1156, 1193, 1647
BlsI GCNGC 6 cut(s) 70, 615, 1017, 1157, 1194, 1648
Bme1390I CCNGG 3 cut(s) 496, 497, 1115
BmeRI GACNNNNNGTC 1 cut(s) 392
BmeT110I CYCGRG 2 cut(s) 102, 495
BmiI GGNNCC 1 cut(s) 791
BmrFI CCNGG 3 cut(s) 496, 497, 1115
BmrI ACTGGG 2 cut(s) 254, 426
BmsI GCATC 1 cut(s) 1548
BmuI ACTGGG 2 cut(s) 254, 426
Bpu14I TTCGAA 1 cut(s) 504
BpuEI CTTGAG 2 cut(s) 335, 1505
BpuMI CCSGG 2 cut(s) 496, 497
BsaAI YACGTR 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 1668
BsaJI CCNNGG 5 cut(s) 495, 603, 1113, 1216, 1246
BsaXI ACNNNNNCTCC 4 cut(s) 247, 277, 900, 930
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 245, 495
Bse1I ACTGG 3 cut(s) 249, 421, 988
Bse8I GATNNNNATC 1 cut(s) 1668
BseBI CCWGG 1 cut(s) 1115
BseDI CCNNGG 5 cut(s) 495, 603, 1113, 1216, 1246
BseGI GGATG 4 cut(s) 192, 438, 613, 1597
BseJI GATNNNNATC 1 cut(s) 1668
BseLI CCNNNNNNNGG 3 cut(s) 244, 245, 495
BseNI ACTGG 3 cut(s) 249, 421, 988
BseRI GAGGAG 3 cut(s) 90, 1508, 1545
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 4 cut(s) 55, 625, 1204, 1658
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 2 cut(s) 405, 1015
Bsh1285I CGRYCG 1 cut(s) 1288
BshFI GGCC 4 cut(s) 121, 987, 1018, 1163
BshNI GGYRCC 1 cut(s) 789
BsiEI CGRYCG 1 cut(s) 1288
BsiHKCI CYCGRG 2 cut(s) 102, 495
BsiSI CCGG 1 cut(s) 496
BslFI GGGAC 3 cut(s) 261, 399, 749
BslI CCNNNNNNNGG 3 cut(s) 244, 245, 495
BsmAI GTCTC 1 cut(s) 1615
BsmFI GGGAC 3 cut(s) 261, 399, 749
BsnI GGCC 4 cut(s) 121, 987, 1018, 1163
BsoBI CYCGRG 2 cut(s) 102, 495
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 9 cut(s) 27, 66, 267, 403, 489, 648, 1015, 1155, 1382
BspANI GGCC 4 cut(s) 121, 987, 1018, 1163
BspFNI CGCG 2 cut(s) 405, 1015
BspLI GGNNCC 1 cut(s) 791
BspMI ACCTGC 1 cut(s) 1265
BspPI GGATC 2 cut(s) 1511, 1664
BspT104I TTCGAA 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 789
BsrBI CCGCTC 1 cut(s) 1382
BsrI ACTGG 3 cut(s) 249, 421, 988
BssECI CCNNGG 5 cut(s) 495, 603, 1113, 1216, 1246
BssMI GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
BssNAI GTATAC 1 cut(s) 1181
BssT1I CCWWGG 2 cut(s) 603, 1246
Bst1107I GTATAC 1 cut(s) 1181
Bst2UI CCWGG 1 cut(s) 1115
Bst4CI ACNGT 3 cut(s) 142, 789, 1587
BstBAI YACGTR 1 cut(s) 466
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 4 cut(s) 123, 403, 1264, 1468
BstDSI CCRYGG 2 cut(s) 603, 1216
BstF5I GGATG 4 cut(s) 192, 438, 613, 1597
BstFNI CGCG 2 cut(s) 405, 1015
BstH2I RGCGCY 1 cut(s) 1443
BstHHI GCGC 3 cut(s) 45, 1015, 1442
BstKTI GATC 6 cut(s) 1024, 1288, 1332, 1506, 1516, 1672
BstMAI GTCTC 1 cut(s) 1615
BstMBI GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
BstMCI CGRYCG 1 cut(s) 1288
BstMWI GCNNNNNNNGC 3 cut(s) 353, 1201, 1439
BstNI CCWGG 1 cut(s) 1115
BstNSI RCATGY 5 cut(s) 360, 1066, 1187, 1266, 1470
BstSCI CCNGG 3 cut(s) 494, 495, 1113
BstSLI GKGCMC 1 cut(s) 423
BstSNI TACGTA 1 cut(s) 466
BstUI CGCG 2 cut(s) 405, 1015
BstV1I GCAGC 4 cut(s) 55, 625, 1204, 1658
BstX2I RGATCY 1 cut(s) 1021
BstYI RGATCY 1 cut(s) 1021
BstZ17I GTATAC 1 cut(s) 1181
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 4 cut(s) 121, 987, 1018, 1163
BtgI CCRYGG 2 cut(s) 603, 1216
BtsCI GGATG 4 cut(s) 192, 438, 613, 1597
BtsI GCAGTG 2 cut(s) 750, 885
BtsIMutI CAGTG 3 cut(s) 750, 885, 995
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 4 cut(s) 123, 403, 1264, 1468
CfoI GCGC 3 cut(s) 45, 1015, 1442
Cfr9I CCCGGG 1 cut(s) 495
CseI GACGC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 165, 790, 1143
CviQI GTAC 3 cut(s) 165, 790, 1143
DpnI GATC 6 cut(s) 1023, 1287, 1331, 1505, 1515, 1671
DpnII GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
DraI TTTAAA 2 cut(s) 672, 1108
DriI GACNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 3 cut(s) 119, 1016, 1161
Eam1105I GACNNNNNGTC 1 cut(s) 392
EciI GGCGGA 1 cut(s) 16
Eco105I TACGTA 1 cut(s) 466
Eco130I CCWWGG 2 cut(s) 603, 1246
Eco88I CYCGRG 2 cut(s) 102, 495
EcoRI GAATTC 1 cut(s) 997
EcoRII CCWGG 1 cut(s) 1113
EcoT14I CCWWGG 2 cut(s) 603, 1246
EcoT22I ATGCAT 2 cut(s) 1054, 1468
ErhI CCWWGG 2 cut(s) 603, 1246
FaqI GGGAC 3 cut(s) 261, 399, 749
FauI CCCGC 3 cut(s) 274, 410, 1375
FblI GTMKAC 1 cut(s) 1180
Fnu4HI GCNGC 6 cut(s) 69, 614, 1016, 1156, 1193, 1647
FokI GGATG 4 cut(s) 179, 425, 620, 1604
Fsp4HI GCNGC 6 cut(s) 69, 614, 1016, 1156, 1193, 1647
FspBI CTAG 4 cut(s) 834, 1211, 1280, 1361
FspI TGCGCA 1 cut(s) 44
GlaI GCGC 3 cut(s) 44, 1014, 1441
GluI GCNGC 6 cut(s) 69, 614, 1016, 1156, 1193, 1647
HaeII RGCGCY 1 cut(s) 1443
HaeIII GGCC 4 cut(s) 121, 987, 1018, 1163
HapII CCGG 1 cut(s) 496
HgaI GACGC 1 cut(s) 394
HhaI GCGC 3 cut(s) 45, 1015, 1442
Hin6I GCGC 3 cut(s) 43, 1013, 1440
HinP1I GCGC 3 cut(s) 43, 1013, 1440
HinfI GANTC 2 cut(s) 506, 1222
HpaII CCGG 1 cut(s) 496
HphI GGTGA 3 cut(s) 790, 1006, 1091
Hpy166II GTNNAC 5 cut(s) 397, 537, 785, 1181, 1216
Hpy188I TCNGA 8 cut(s) 86, 93, 132, 430, 718, 1329, 1508, 1544
Hpy188III TCNNGA 6 cut(s) 104, 893, 1229, 1522, 1580, 1610
Hpy8I GTNNAC 5 cut(s) 397, 537, 785, 1181, 1216
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 7 cut(s) 30, 40, 44, 346, 445, 1252, 1621
HpyCH4III ACNGT 3 cut(s) 142, 789, 1587
HpyCH4IV ACGT 5 cut(s) 9, 175, 465, 1131, 1353
HpyCH4V TGCA 9 cut(s) 613, 743, 854, 1052, 1122, 1192, 1266, 1466, 1470
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 1201, 1439
HpySE526I ACGT 5 cut(s) 9, 175, 465, 1131, 1353
HspAI GCGC 3 cut(s) 43, 1013, 1440
KpnI GGTACC 1 cut(s) 793
Kzo9I GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
LmnI GCTCC 4 cut(s) 309, 665, 971, 1451
Lsp1109I GCAGC 4 cut(s) 55, 625, 1204, 1658
LweI GCATC 1 cut(s) 1548
MaeI CTAG 4 cut(s) 834, 1211, 1280, 1361
MaeII ACGT 5 cut(s) 9, 175, 465, 1131, 1353
MaeIII GTNAC 3 cut(s) 466, 1237, 1268
MalI GATC 6 cut(s) 1023, 1287, 1331, 1505, 1515, 1671
MbiI CCGCTC 1 cut(s) 1382
MboI GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
MboII GAAGA 3 cut(s) 954, 1501, 1648
MfeI CAATTG 2 cut(s) 76, 585
MflI RGATCY 1 cut(s) 1021
MhlI GDGCHC 1 cut(s) 423
MlsI TGGCCA 2 cut(s) 121, 1163
MluCI AATT 9 cut(s) 52, 76, 126, 179, 585, 619, 997, 1473, 1640
MluNI TGGCCA 2 cut(s) 121, 1163
MmeI TCCRAC 1 cut(s) 729
Mox20I TGGCCA 2 cut(s) 121, 1163
Mph1103I ATGCAT 2 cut(s) 1054, 1468
MscI TGGCCA 2 cut(s) 121, 1163
MseI TTAA 9 cut(s) 182, 233, 558, 591, 671, 1055, 1107, 1413, 1461
MslI CAYNNNNRTG 4 cut(s) 608, 641, 1163, 1482
Msp20I TGGCCA 2 cut(s) 121, 1163
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 496
MspR9I CCNGG 3 cut(s) 496, 497, 1115
MunI CAATTG 2 cut(s) 76, 585
MvaI CCWGG 1 cut(s) 1115
MvnI CGCG 2 cut(s) 405, 1015
MwoI GCNNNNNNNGC 3 cut(s) 353, 1201, 1439
NciI CCSGG 2 cut(s) 496, 497
NcoI CCATGG 1 cut(s) 603
NdeII GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
NlaIV GGNNCC 1 cut(s) 791
NmuCI GTSAC 1 cut(s) 1268
NsbI TGCGCA 1 cut(s) 44
NsiI ATGCAT 2 cut(s) 1054, 1468
NspI RCATGY 5 cut(s) 360, 1066, 1187, 1266, 1470
NspV TTCGAA 1 cut(s) 504
PaeI GCATGC 2 cut(s) 1266, 1470
PaeR7I CTCGAG 1 cut(s) 102
PciI ACATGT 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 3 cut(s) 405, 539, 1578
PfeI GAWTC 2 cut(s) 506, 1222
PkrI GCNGC 6 cut(s) 70, 615, 1017, 1157, 1194, 1648
Ple19I CGATCG 1 cut(s) 1288
Ppu21I YACGTR 1 cut(s) 466
PscI ACATGT 1 cut(s) 1062
PshBI ATTAAT 1 cut(s) 1055
PsiI TTATAA 1 cut(s) 1684
Psp1406I AACGTT 1 cut(s) 1131
Psp6I CCWGG 1 cut(s) 1113
PspGI CCWGG 1 cut(s) 1113
PspN4I GGNNCC 1 cut(s) 791
PsuI RGATCY 1 cut(s) 1021
PvuI CGATCG 1 cut(s) 1288
RsaI GTAC 3 cut(s) 166, 791, 1144
RsaNI GTAC 3 cut(s) 165, 790, 1143
RseI CAYNNNNRTG 4 cut(s) 608, 641, 1163, 1482
SaqAI TTAA 9 cut(s) 182, 233, 558, 591, 671, 1055, 1107, 1413, 1461
SatI GCNGC 6 cut(s) 69, 614, 1016, 1156, 1193, 1647
Sau3AI GATC 6 cut(s) 1021, 1285, 1329, 1503, 1513, 1669
ScrFI CCNGG 3 cut(s) 496, 497, 1115
SduI GDGCHC 1 cut(s) 423
SfaNI GCATC 1 cut(s) 1548
Sfr274I CTCGAG 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 504
SlaI CTCGAG 1 cut(s) 102
SmaI CCCGGG 1 cut(s) 497
SmiMI CAYNNNNRTG 4 cut(s) 608, 641, 1163, 1482
SmlI CTYRAG 3 cut(s) 102, 350, 1520
SmoI CTYRAG 3 cut(s) 102, 350, 1520
SnaBI TACGTA 1 cut(s) 466
SphI GCATGC 2 cut(s) 1266, 1470
Sse9I AATT 9 cut(s) 52, 76, 126, 179, 585, 619, 997, 1473, 1640
SsiI CCGC 9 cut(s) 27, 66, 267, 403, 489, 648, 1015, 1155, 1382
SspMI CTAG 4 cut(s) 834, 1211, 1280, 1361
StyD4I CCNGG 3 cut(s) 494, 495, 1113
StyI CCWWGG 2 cut(s) 603, 1246
TaaI ACNGT 3 cut(s) 142, 789, 1587
TaiI ACGT 5 cut(s) 12, 178, 468, 1134, 1356
TaqI TCGA 9 cut(s) 103, 504, 542, 656, 965, 1284, 1288, 1378, 1562
TasI AATT 9 cut(s) 52, 76, 126, 179, 585, 619, 997, 1473, 1640
TatI WGTACW 1 cut(s) 164
TauI GCSGC 2 cut(s) 1018, 1158
TfiI GAWTC 2 cut(s) 506, 1222
Tru1I TTAA 9 cut(s) 182, 233, 558, 591, 671, 1055, 1107, 1413, 1461
Tru9I TTAA 9 cut(s) 182, 233, 558, 591, 671, 1055, 1107, 1413, 1461
TscAI CASTG 3 cut(s) 750, 885, 995
TseFI GTSAC 1 cut(s) 1268
TseI GCWGC 4 cut(s) 68, 613, 1192, 1646
Tsp45I GTSAC 1 cut(s) 1268
TspDTI ATGAA 9 cut(s) 185, 427, 446, 680, 1181, 1328, 1399, 1466, 1629
TspMI CCCGGG 1 cut(s) 495
TspRI CASTG 3 cut(s) 750, 885, 995
VspI ATTAAT 1 cut(s) 1055
XapI RAATTY 3 cut(s) 52, 997, 1473
XceI RCATGY 5 cut(s) 360, 1066, 1187, 1266, 1470
XhoI CTCGAG 1 cut(s) 102
XmaI CCCGGG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 1180
XspI CTAG 4 cut(s) 834, 1211, 1280, 1361
Zsp2I ATGCAT 2 cut(s) 1054, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.