Rroxscaffold_5G00351430

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001684
Physical Location & Seq
Forward (+)
27664471 .. 27665428
958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351430.1

Sequence Viewer

Length: 771 bp
ATGTCCATGGACGCAGAACCCAATGTTACTACTCTTGTGACTGTTTTGTCTTCGTGTTCTAGTTTAAGAGCTTTCAAGTTAGGTAAAGCTGTTCATGGCCATTGTTTGAGGAACGTACACAAGTGTAATATGATTTTGGACAATGCAATGTTGGATTTCTATCTGAGATGTGGTTCTTTGGTGGCTGCAAGATACTTGTTTGTCATTATGCCCAAGAGAGATTTTGTTTCTTGGAGTAGCATGGTGGGTGGTCATGCACATAGATGTGAGGAGGCAATGAGACTTTTCCAACAACTGGTGCTGGGCTGGGAAGTTGAGCCGAATGAGGCTACCATTGTCAATGTATTGTCAGCATGTTTCTCTGTTTGTTCCTTGAGTTCAAGCCAGCAGGTGCATGTCTATATCAGTACTCAGCCAGATCTCATGGTGAATAGCAATGTGGGAAATGCCTTGATCAACATGTATGTTAGGAGTGGACATTTGGTCAGTGGGGTTTCTCCTGATGGTATAACCTTCCTTGGGTTATTAACAGCATGCAGTCATGCAGGCCTTGTAAACCAAGAGTTGATGTTCTTCAATGCTATGAAGAAAGCTTTTATTAAAGAAATGCCTTTGAAAGCTGATGGTTCTGTTTGGGGAGCTCTGCTTAGTGCTTGCAAAATTCATGAGAATGAGATGATGATTGAGAGAATCAGACAAGGACTTCCAAATAATGCAGGAGTTAGCATTGGAACTTATGCTCTGTTATCAAATGCTGTTGCGTTGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

256

Amino Acids

27.9

Weight (kDa)

7.61

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 379
AasI GACNNNNNNGTC 1 cut(s) 46
Acc36I ACCTGC 1 cut(s) 379
AccB7I CCANNNNNTGG 1 cut(s) 295
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 1 cut(s) 660
AfaI GTAC 2 cut(s) 117, 409
AfiI CCNNNNNNNGG 2 cut(s) 295, 519
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 4 cut(s) 76, 381, 577, 616
AhdI GACNNNNNGTC 1 cut(s) 482
AluBI AGCT 5 cut(s) 71, 89, 593, 620, 641
AluI AGCT 5 cut(s) 71, 89, 593, 620, 641
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 1 cut(s) 274
AoxI GGCC 2 cut(s) 97, 547
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 660
AsuHPI GGTGA 1 cut(s) 439
BalI TGGCCA 1 cut(s) 99
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 497, 617
BclI TGATCA 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 418
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
BmcAI AGTACT 1 cut(s) 409
BmeRI GACNNNNNGTC 1 cut(s) 482
BpiI GAAGAC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 394
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 6, 517
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 519
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 3 cut(s) 153, 282, 442
Bse8I GATNNNNATC 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 6, 517
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 295, 519
BseMI GCAATG 3 cut(s) 153, 282, 442
BseMII CTCAG 2 cut(s) 155, 425
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 1 cut(s) 172
BseYI CCCAGC 2 cut(s) 301, 306
BshFI GGCC 2 cut(s) 99, 549
BsiHKAI GWGCWC 1 cut(s) 643
BslI CCNNNNNNNGG 2 cut(s) 295, 519
BsmAI GTCTC 1 cut(s) 274
BsnI GGCC 2 cut(s) 99, 549
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 2 cut(s) 418, 453
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 2 cut(s) 99, 549
BspCNI CTCAG 2 cut(s) 156, 424
BspHI TCATGA 1 cut(s) 664
BspMI ACCTGC 1 cut(s) 379
BsrDI GCAATG 3 cut(s) 153, 282, 442
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 2 cut(s) 6, 517
BssMI GATC 2 cut(s) 418, 453
BssT1I CCWWGG 2 cut(s) 6, 517
Bst4CI ACNGT 1 cut(s) 43
BstC8I GCNNGC 4 cut(s) 386, 535, 547, 655
BstDEI CTNAG 3 cut(s) 164, 411, 647
BstDSI CCRYGG 1 cut(s) 6
BstKTI GATC 2 cut(s) 421, 456
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 2 cut(s) 418, 453
BstNSI RCATGY 4 cut(s) 357, 398, 463, 537
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 2 cut(s) 99, 549
BtgI CCRYGG 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 493
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 4 cut(s) 386, 535, 547, 655
CciI TCATGA 1 cut(s) 664
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 116, 408
CviQI GTAC 2 cut(s) 116, 408
DdeI CTNAG 3 cut(s) 164, 411, 647
DpnI GATC 2 cut(s) 420, 455
DpnII GATC 2 cut(s) 418, 453
DrdI GACNNNNNNGTC 1 cut(s) 46
DriI GACNNNNNGTC 1 cut(s) 482
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 97
Eam1105I GACNNNNNGTC 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 641
Eco130I CCWWGG 2 cut(s) 6, 517
Eco147I AGGCCT 1 cut(s) 549
Eco24I GRGCYC 1 cut(s) 643
Eco53kI GAGCTC 1 cut(s) 641
EcoICRI GAGCTC 1 cut(s) 641
EcoT14I CCWWGG 2 cut(s) 6, 517
EcoT38I GRGCYC 1 cut(s) 643
ErhI CCWWGG 2 cut(s) 6, 517
FbaI TGATCA 1 cut(s) 453
Fnu4HI GCNGC 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 1 cut(s) 60
GluI GCNGC 1 cut(s) 186
GsaI CCCAGC 2 cut(s) 305, 310
HaeIII GGCC 2 cut(s) 99, 549
HgaI GACGC 1 cut(s) 20
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 1 cut(s) 690
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 3 cut(s) 118, 476, 556
Hpy188I TCNGA 2 cut(s) 165, 695
Hpy188III TCNNGA 2 cut(s) 500, 665
Hpy8I GTNNAC 3 cut(s) 118, 476, 556
HpyAV CCTTC 1 cut(s) 523
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 8 cut(s) 146, 188, 257, 394, 537, 545, 657, 716
HpyF3I CTNAG 3 cut(s) 164, 411, 647
HpySE526I ACGT 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 2 cut(s) 418, 453
LmnI GCTCC 1 cut(s) 638
LpnPI CCDG 9 cut(s) 281, 287, 292, 374, 398, 429, 513, 531, 702
Lsp1109I GCAGC 1 cut(s) 172
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 25, 37
MalI GATC 2 cut(s) 420, 455
MboI GATC 2 cut(s) 418, 453
MboII GAAGA 3 cut(s) 42, 565, 598
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 1 cut(s) 660
MluNI TGGCCA 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 132, 313
MnlI CCTC 4 cut(s) 102, 262, 265, 319
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MseI TTAA 3 cut(s) 65, 527, 600
MslI CAYNNNNRTG 3 cut(s) 262, 264, 669
Msp20I TGGCCA 1 cut(s) 99
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 418, 453
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 4 cut(s) 357, 398, 463, 537
PaeI GCATGC 1 cut(s) 537
PagI TCATGA 1 cut(s) 664
PaqCI CACCTGC 1 cut(s) 379
PceI AGGCCT 1 cut(s) 549
PciI ACATGT 1 cut(s) 459
PfeI GAWTC 1 cut(s) 690
PflMI CCANNNNNTGG 1 cut(s) 295
PkrI GCNGC 1 cut(s) 187
PscI ACATGT 1 cut(s) 459
Psp124BI GAGCTC 1 cut(s) 643
PspFI CCCAGC 2 cut(s) 301, 306
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 2 cut(s) 117, 409
RsaNI GTAC 2 cut(s) 116, 408
RseI CAYNNNNRTG 3 cut(s) 262, 264, 669
SacI GAGCTC 1 cut(s) 643
SaqAI TTAA 3 cut(s) 65, 527, 600
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 418, 453
ScaI AGTACT 1 cut(s) 409
SduI GDGCHC 1 cut(s) 643
SetI ASST 9 cut(s) 73, 85, 91, 117, 393, 515, 595, 622, 643
SmiMI CAYNNNNRTG 3 cut(s) 262, 264, 669
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
SphI GCATGC 1 cut(s) 537
Sse9I AATT 1 cut(s) 660
SseBI AGGCCT 1 cut(s) 549
SspMI CTAG 1 cut(s) 60
SstI GAGCTC 1 cut(s) 643
StuI AGGCCT 1 cut(s) 549
StyI CCWWGG 2 cut(s) 6, 517
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 117
TasI AATT 1 cut(s) 660
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 1 cut(s) 690
Tru1I TTAA 3 cut(s) 65, 527, 600
Tru9I TTAA 3 cut(s) 65, 527, 600
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 83, 599, 653
TspRI CASTG 1 cut(s) 493
Van91I CCANNNNNTGG 1 cut(s) 295
XapI RAATTY 1 cut(s) 660
XceI RCATGY 4 cut(s) 357, 398, 463, 537
XspI CTAG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.