Rw7G035240

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001684
Physical Location & Seq
Forward (+)
52543381 .. 52544955
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035240.1

Sequence Viewer

Length: 1464 bp
ATGAAACAACTAAACCAAATCCATGCGCTGCTGGTCAAAAATCCAAAACCCCAAGTTTTAAACCCCTGGCTGGGATATCTGACAAAGTCTTCCGCACCACAAAATGCATGCTGCTTGCTCCATTCACCCCATAAAGGCCAAGAAATTCAAGCCCACGTCACAAAATCCGGTCACATTTCTGATACCTTCATCCAAAACTCTTTGCTCCACTTTTACGTAATCCAATCTGACATTGTTTCTGCTACCCGCGTTTTTGATTCAATACCCATACCGGATGTTGTTTCCTGGACTTCGATGATTTCGGGTCTTTCCAAGTGTGGGTTTGTGGAGGAAGCCATTGTGAAGTTTATGTCCATGGACGTGAAGCCTAATTCCACTACTCTTGTGACTGTTTTGTCTTCTTGTTCTACTTTAAGAGCTTTGAAATTTGGTAAAGCTGTTCATGGCCATTGTTTGAGGAACTTTCGGGAAAGAAATTTGATTTTGGACAATGCGGTGTTGGATTTCTATCTGAGATGTGGATCTTTGGCGAGTGCACGATACCTGTTTGTCAATATGCCCGAGAGAGATGTGGTTTCTTGGACTATCATGGTGGGTGGTTATGCACAAAGAGGTTTTTGTGAAGAGGCAGTGAAACTTTTCCAACAACTGCTGCAGGGTGGAGAAGCTGAGTCTAATGAGGCTACCATTGTCAATGTATTGTCAGCATGTGCAAATGGCAATGTGGGGAATGCCTTGATCAACATGTATGTGAAGTGTGGAAACTTGGAAATGGCTATCTCAGTTTTTCAGTCCCTGGAGCACAAAGATATCATTTCATGGAGTACCATAATATGCGGCATGGCCATGAATGGCCATGGCATACACGTGTTGCAGCTCTTTTCCAGTATGCTAGTCAATGGGGTGTCTCCTGATGGTGTAACCTTCCTCGGGTTGTTATCAGCATGCAGTCATGCTGGCCTGGTAAATCAAGGGTTGATGTTCTTCAATGCTATGAAGAAAGTCTACCGGATTGTCCCTGAAACACAGCATTATGCGTGTCTGGTTGATATGTATGGTCGAGCTGCGCTATTAGAGGAAGCAGAGGCTTTTATTAAAGAAATGCCAACAGAAGCTGATGGTCCTGTTTGGGAAGCGCTGCTTAATGCTTGTAAAATCTATGGGAATGAGGAGATGGTTGAGAGGATCGGAGAAGGCCTTCGCAATAGTGCAGGAGTGAGCACCGGAACTTATGCTCTGTTATCAAATGCTTATGCTAAACATGATAGGTGGGATGATTCTAATAAGGTTCGTGATGAAATGAGAGAGATGGGGTTGAAGGCATGCCACCTGGTCGTAGTTGGATTGAAATTGGTCCGTCCATCTAACCTATACACACGTGATCCCAACATGGTCATCATATGGTCATACCCAAGAACCACTTTTTGTCTAACCAGACCCCATGATGGGTTTTTAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

487

Amino Acids

53.94

Weight (kDa)

6.75

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 91 - 135 9.7e-06 PPR repeat family
PPR PF01535 192 - 219 7.4e-06 PPR repeat
PPR_2 PF13041 269 - 317 1.1e-07 PPR repeat family
E_motif PF20431 407 - 440 8.3e-06 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 394
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 1 cut(s) 249
AciI CCGC 4 cut(s) 93, 247, 494, 837
AclWI GGATC 3 cut(s) 529, 1193, 1376
AcoI YGGCCR 3 cut(s) 445, 843, 853
AcsI RAATTY 4 cut(s) 144, 425, 475, 1458
AcvI CACGTG 2 cut(s) 868, 1379
AfaI GTAC 1 cut(s) 826
AfeI AGCGCT 1 cut(s) 1137
AfiI CCNNNNNNNGG 7 cut(s) 70, 71, 134, 318, 930, 1445, 1446
AflIII ACRYGT 4 cut(s) 744, 865, 867, 1376
AgsI TTSAA 6 cut(s) 149, 261, 424, 988, 1318, 1348
AjiI CACGTC 2 cut(s) 157, 361
AjnI CCWGG 5 cut(s) 65, 284, 795, 960, 1329
AluBI AGCT 6 cut(s) 419, 437, 668, 877, 1064, 1115
AluI AGCT 6 cut(s) 419, 437, 668, 877, 1064, 1115
Alw21I GWGCWC 3 cut(s) 538, 804, 1223
Alw26I GTCTC 1 cut(s) 912
Alw44I GTGCAC 1 cut(s) 534
AlwI GGATC 3 cut(s) 529, 1193, 1376
AlwNI CAGNNNCTG 2 cut(s) 796, 1115
Ama87I CYCGRG 2 cut(s) 560, 929
Aor51HI AGCGCT 1 cut(s) 1137
AoxI GGCC 6 cut(s) 136, 445, 843, 853, 958, 1195
ApaLI GTGCAC 1 cut(s) 534
ApeKI GCWGC 6 cut(s) 28, 111, 652, 874, 1064, 1138
ApoI RAATTY 4 cut(s) 144, 425, 475, 1458
Asp700I GAANNNNTTC 2 cut(s) 638, 1197
AspLEI GCGC 3 cut(s) 28, 1069, 1138
AspS9I GGNCC 2 cut(s) 1121, 1354
AsuHPI GGTGA 1 cut(s) 117
AvaI CYCGRG 2 cut(s) 560, 929
AvaII GGWCC 2 cut(s) 1121, 1354
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 3 cut(s) 447, 845, 855
BarI GAAGNNNNNNTAC 2 cut(s) 989, 1021
BbrPI CACGTG 2 cut(s) 868, 1379
BbsI GAAGAC 2 cut(s) 81, 390
Bbv12I GWGCWC 3 cut(s) 538, 804, 1223
BbvI GCAGC 6 cut(s) 15, 98, 639, 886, 1051, 1125
BccI CCATC 6 cut(s) 908, 1112, 1168, 1303, 1369, 1439
BciT130I CCWGG 5 cut(s) 67, 286, 797, 962, 1331
BclI TGATCA 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 1 cut(s) 653
BfoI RGCGCY 1 cut(s) 1139
BisI GCNGC 7 cut(s) 29, 112, 653, 838, 875, 1065, 1139
BlsI GCNGC 7 cut(s) 30, 113, 654, 839, 876, 1066, 1140
Bme1390I CCNGG 5 cut(s) 67, 286, 797, 962, 1331
Bme18I GGWCC 2 cut(s) 1121, 1354
BmeT110I CYCGRG 2 cut(s) 560, 929
BmgBI CACGTC 2 cut(s) 157, 361
BmgT120I GGNCC 2 cut(s) 1121, 1354
BmrFI CCNGG 5 cut(s) 67, 286, 797, 962, 1331
BpiI GAAGAC 2 cut(s) 81, 390
BpmI CTGGAG 1 cut(s) 818
BsaAI YACGTR 3 cut(s) 217, 868, 1379
BsaBI GATNNNNATC 2 cut(s) 507, 520
BsaJI CCNNGG 5 cut(s) 65, 354, 795, 856, 928
BsaWI WCCGGW 4 cut(s) 167, 271, 1008, 1223
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 71, 134, 318, 930, 1445, 1446
Bse1I ACTGG 1 cut(s) 885
Bse3DI GCAATG 1 cut(s) 727
Bse8I GATNNNNATC 2 cut(s) 507, 520
BseBI CCWGG 5 cut(s) 67, 286, 797, 962, 1331
BseDI CCNNGG 5 cut(s) 65, 354, 795, 856, 928
BseGI GGATG 3 cut(s) 189, 280, 1279
BseJI GATNNNNATC 2 cut(s) 507, 520
BseLI CCNNNNNNNGG 7 cut(s) 70, 71, 134, 318, 930, 1445, 1446
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 3 cut(s) 503, 660, 795
BseNI ACTGG 1 cut(s) 885
BseRI GAGGAG 1 cut(s) 1184
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 6 cut(s) 15, 98, 639, 886, 1051, 1125
BseYI CCCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 6 cut(s) 138, 447, 845, 855, 960, 1197
BsiHKAI GWGCWC 3 cut(s) 538, 804, 1223
BsiHKCI CYCGRG 2 cut(s) 560, 929
BsiSI CCGG 4 cut(s) 168, 272, 1009, 1224
BslFI GGGAC 2 cut(s) 778, 1001
BslI CCNNNNNNNGG 7 cut(s) 70, 71, 134, 318, 930, 1445, 1446
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 778, 1001
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 6 cut(s) 138, 447, 845, 855, 960, 1197
BsoBI CYCGRG 2 cut(s) 560, 929
Bsp1286I GDGCHC 3 cut(s) 538, 804, 1223
Bsp143I GATC 4 cut(s) 521, 738, 1185, 1381
Bsp19I CCATGG 2 cut(s) 354, 856
BspACI CCGC 4 cut(s) 93, 247, 494, 837
BspANI GGCC 6 cut(s) 138, 447, 845, 855, 960, 1197
BspCNI CTCAG 3 cut(s) 504, 661, 794
BspFNI CGCG 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 657
BspPI GGATC 3 cut(s) 529, 1193, 1376
BsrDI GCAATG 1 cut(s) 727
BsrI ACTGG 1 cut(s) 885
BssECI CCNNGG 5 cut(s) 65, 354, 795, 856, 928
BssMI GATC 4 cut(s) 521, 738, 1185, 1381
BssT1I CCWWGG 2 cut(s) 354, 856
Bst2UI CCWGG 5 cut(s) 67, 286, 797, 962, 1331
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 618
BstBAI YACGTR 3 cut(s) 217, 868, 1379
BstC8I GCNNGC 5 cut(s) 109, 116, 946, 958, 1324
BstDEI CTNAG 3 cut(s) 512, 669, 781
BstDSI CCRYGG 2 cut(s) 354, 856
BstF5I GGATG 3 cut(s) 189, 280, 1279
BstFNI CGCG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 1139
BstHHI GCGC 3 cut(s) 28, 1069, 1138
BstKTI GATC 4 cut(s) 524, 741, 1188, 1384
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 4 cut(s) 521, 738, 1185, 1381
BstNI CCWGG 5 cut(s) 67, 286, 797, 962, 1331
BstNSI RCATGY 5 cut(s) 111, 711, 748, 948, 1326
BstSCI CCNGG 5 cut(s) 65, 284, 795, 960, 1329
BstSFI CTRYAG 1 cut(s) 653
BstSLI GKGCMC 1 cut(s) 538
BstSNI TACGTA 1 cut(s) 217
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 6 cut(s) 15, 98, 639, 886, 1051, 1125
BstV2I GAAGAC 2 cut(s) 81, 390
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
BsuRI GGCC 6 cut(s) 138, 447, 845, 855, 960, 1197
BtgI CCRYGG 2 cut(s) 354, 856
BtrI CACGTC 2 cut(s) 157, 361
BtsCI GGATG 3 cut(s) 189, 280, 1279
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 636
Cac8I GCNNGC 5 cut(s) 109, 116, 946, 958, 1324
CaiI CAGNNNCTG 2 cut(s) 796, 1115
CfoI GCGC 3 cut(s) 28, 1069, 1138
Cfr13I GGNCC 2 cut(s) 1121, 1354
CsiI ACCWGGT 1 cut(s) 1329
Csp6I GTAC 1 cut(s) 825
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviQI GTAC 1 cut(s) 825
DdeI CTNAG 3 cut(s) 512, 669, 781
DpnI GATC 4 cut(s) 523, 740, 1187, 1383
DpnII GATC 4 cut(s) 521, 738, 1185, 1381
DraI TTTAAA 1 cut(s) 60
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 445, 843, 853
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco105I TACGTA 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 354, 856
Eco147I AGGCCT 1 cut(s) 1197
Eco32I GATATC 2 cut(s) 77, 811
Eco47I GGWCC 2 cut(s) 1121, 1354
Eco47III AGCGCT 1 cut(s) 1137
Eco72I CACGTG 2 cut(s) 868, 1379
Eco88I CYCGRG 2 cut(s) 560, 929
EcoRII CCWGG 5 cut(s) 65, 284, 795, 960, 1329
EcoRV GATATC 2 cut(s) 77, 811
EcoT14I CCWWGG 2 cut(s) 354, 856
EcoT22I ATGCAT 1 cut(s) 109
ErhI CCWWGG 2 cut(s) 354, 856
FalI AAGNNNNNCTT 4 cut(s) 1125, 1157, 1405, 1437
FaqI GGGAC 2 cut(s) 778, 1001
FauI CCCGC 1 cut(s) 254
FauNDI CATATG 1 cut(s) 1400
FbaI TGATCA 1 cut(s) 738
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 7 cut(s) 29, 112, 653, 838, 875, 1065, 1139
FokI GGATG 3 cut(s) 176, 287, 1286
Fsp4HI GCNGC 7 cut(s) 29, 112, 653, 838, 875, 1065, 1139
FspBI CTAG 1 cut(s) 893
GlaI GCGC 3 cut(s) 27, 1068, 1137
GluI GCNGC 7 cut(s) 29, 112, 653, 838, 875, 1065, 1139
GsaI CCCAGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 818
HaeII RGCGCY 1 cut(s) 1139
HaeIII GGCC 6 cut(s) 138, 447, 845, 855, 960, 1197
HapII CCGG 4 cut(s) 168, 272, 1009, 1224
HhaI GCGC 3 cut(s) 28, 1069, 1138
Hin6I GCGC 3 cut(s) 26, 1067, 1136
HinP1I GCGC 3 cut(s) 26, 1067, 1136
HinfI GANTC 3 cut(s) 257, 671, 1277
HpaII CCGG 4 cut(s) 168, 272, 1009, 1224
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 2 cut(s) 536, 1006
Hpy188I TCNGA 5 cut(s) 81, 181, 229, 513, 1190
Hpy188III TCNNGA 3 cut(s) 467, 911, 1292
Hpy8I GTNNAC 2 cut(s) 536, 1006
HpyAV CCTTC 5 cut(s) 196, 934, 1187, 1208, 1312
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 5 cut(s) 156, 216, 360, 867, 1378
HpyCH4V TGCA 8 cut(s) 107, 536, 605, 655, 713, 874, 948, 1211
HpyF3I CTNAG 3 cut(s) 512, 669, 781
HpySE526I ACGT 5 cut(s) 156, 216, 360, 867, 1378
HspAI GCGC 3 cut(s) 26, 1067, 1136
Ksp22I TGATCA 1 cut(s) 738
Kzo9I GATC 4 cut(s) 521, 738, 1185, 1381
LmnI GCTCC 3 cut(s) 123, 210, 799
Lsp1109I GCAGC 6 cut(s) 15, 98, 639, 886, 1051, 1125
MabI ACCWGGT 1 cut(s) 1329
MaeI CTAG 1 cut(s) 893
MaeII ACGT 5 cut(s) 156, 216, 360, 867, 1378
MaeIII GTNAC 4 cut(s) 157, 170, 385, 919
MalI GATC 4 cut(s) 523, 740, 1187, 1383
MboI GATC 4 cut(s) 521, 738, 1185, 1381
MboII GAAGA 5 cut(s) 81, 390, 635, 976, 1009
MflI RGATCY 1 cut(s) 521
MhlI GDGCHC 3 cut(s) 538, 804, 1223
MlsI TGGCCA 3 cut(s) 447, 845, 855
MluCI AATT 6 cut(s) 144, 370, 425, 475, 1349, 1458
MluNI TGGCCA 3 cut(s) 447, 845, 855
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 3 cut(s) 480, 667, 1321
Mox20I TGGCCA 3 cut(s) 447, 845, 855
Mph1103I ATGCAT 1 cut(s) 109
MroXI GAANNNNTTC 2 cut(s) 638, 1197
MscI TGGCCA 3 cut(s) 447, 845, 855
MseI TTAA 4 cut(s) 59, 413, 1095, 1143
MslI CAYNNNNRTG 4 cut(s) 359, 749, 845, 866
Msp20I TGGCCA 3 cut(s) 447, 845, 855
MspI CCGG 4 cut(s) 168, 272, 1009, 1224
MspR9I CCNGG 5 cut(s) 67, 286, 797, 962, 1331
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 5 cut(s) 67, 286, 797, 962, 1331
MvnI CGCG 1 cut(s) 249
NcoI CCATGG 2 cut(s) 354, 856
NdeI CATATG 1 cut(s) 1400
NdeII GATC 4 cut(s) 521, 738, 1185, 1381
NmuCI GTSAC 3 cut(s) 157, 170, 385
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 5 cut(s) 111, 711, 748, 948, 1326
PaeI GCATGC 3 cut(s) 111, 948, 1326
PceI AGGCCT 1 cut(s) 1197
PciI ACATGT 1 cut(s) 744
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 638, 1197
PfeI GAWTC 2 cut(s) 257, 1277
PflFI GACNNNGTC 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 7 cut(s) 30, 113, 654, 839, 876, 1066, 1140
PleI GAGTC 1 cut(s) 679
PmaCI CACGTG 2 cut(s) 868, 1379
PmlI CACGTG 2 cut(s) 868, 1379
PpsI GAGTC 1 cut(s) 679
Ppu21I YACGTR 3 cut(s) 217, 868, 1379
PscI ACATGT 1 cut(s) 744
Psp6I CCWGG 5 cut(s) 65, 284, 795, 960, 1329
PspCI CACGTG 2 cut(s) 868, 1379
PspFI CCCAGC 1 cut(s) 70
PspGI CCWGG 5 cut(s) 65, 284, 795, 960, 1329
PspPI GGNCC 2 cut(s) 1121, 1354
PstI CTGCAG 1 cut(s) 657
PstNI CAGNNNCTG 2 cut(s) 796, 1115
PsuI RGATCY 1 cut(s) 521
PsyI GACNNNGTC 1 cut(s) 85
RsaI GTAC 1 cut(s) 826
RsaNI GTAC 1 cut(s) 825
RseI CAYNNNNRTG 4 cut(s) 359, 749, 845, 866
SaqAI TTAA 4 cut(s) 59, 413, 1095, 1143
SatI GCNGC 7 cut(s) 29, 112, 653, 838, 875, 1065, 1139
Sau3AI GATC 4 cut(s) 521, 738, 1185, 1381
Sau96I GGNCC 2 cut(s) 1121, 1354
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 5 cut(s) 67, 286, 797, 962, 1331
SduI GDGCHC 3 cut(s) 538, 804, 1223
SexAI ACCWGGT 1 cut(s) 1329
SfcI CTRYAG 1 cut(s) 653
SinI GGWCC 2 cut(s) 1121, 1354
SmiMI CAYNNNNRTG 4 cut(s) 359, 749, 845, 866
SnaBI TACGTA 1 cut(s) 217
SphI GCATGC 3 cut(s) 111, 948, 1326
Sse9I AATT 6 cut(s) 144, 370, 425, 475, 1349, 1458
SseBI AGGCCT 1 cut(s) 1197
SsiI CCGC 4 cut(s) 93, 247, 494, 837
SspMI CTAG 1 cut(s) 893
StuI AGGCCT 1 cut(s) 1197
StyD4I CCNGG 5 cut(s) 65, 284, 795, 960, 1329
StyI CCWWGG 2 cut(s) 354, 856
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 5 cut(s) 159, 219, 363, 870, 1381
TaqI TCGA 2 cut(s) 293, 1060
TasI AATT 6 cut(s) 144, 370, 425, 475, 1349, 1458
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 2 cut(s) 257, 1277
Tru1I TTAA 4 cut(s) 59, 413, 1095, 1143
Tru9I TTAA 4 cut(s) 59, 413, 1095, 1143
TscAI CASTG 1 cut(s) 636
TseFI GTSAC 3 cut(s) 157, 170, 385
TseI GCWGC 6 cut(s) 28, 111, 652, 874, 1064, 1138
Tsp45I GTSAC 3 cut(s) 157, 170, 385
TspDTI ATGAA 7 cut(s) 17, 178, 431, 807, 863, 1010, 1311
TspGWI ACGGA 1 cut(s) 1346
TspRI CASTG 1 cut(s) 636
Tth111I GACNNNGTC 1 cut(s) 85
VneI GTGCAC 1 cut(s) 534
VpaK11BI GGWCC 2 cut(s) 1121, 1354
XapI RAATTY 4 cut(s) 144, 425, 475, 1458
XceI RCATGY 5 cut(s) 111, 711, 748, 948, 1326
XmiI GTMKAC 1 cut(s) 1005
XmnI GAANNNNTTC 2 cut(s) 638, 1197
XspI CTAG 1 cut(s) 893
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.