MD02G1110200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001684
Physical Location & Seq
Reverse (-)
8944476 .. 8945866
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1110200.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGCTCTACTACCCAACCTCCCACAACCACTACACCTTCACTTACGCTCTCAAGGCATGTTGCTTCCTCCATGATAACAACAAGGGCCGGGAAGTCCATGCCCGCGTCGTAAAAACCGGGCACTTTTCCGATACCTTCATCCAAAACTCGTTGCTTCACTTCTACGTAACCCAAGCTGACATTGTATCTGCTACCCGGGTTTTCGATTCCATACCAAACCTTGATGTTGTTTCGTGGACTTCGATGATTTCGGGTCTTAACAAGTGTAGATTTGTGGAGGAAGCAATTGTTAAGTTTATGTGCATGGATGTGCCGCCTAATTCTACTACTCTTGTCATTGTCATGTCCGCTTGTTCGAGTTTAGGAGCTTTAAAGTTTGGTAAATCTGTTCATGGATATTGTATGAGGAAGTTATCTGAAAGGAATGTTATTTTGGACAATGCAGTGTTGGATTTTTACATGAGATGTGGGTCTTTGGTGGGTGGTTATGCACAAAGAGGATTTTGTGACGAGGCAGTGAAGCTTTTTCAGGAGATGGTTCAGGGAGAGGAAGTTGAGCCTAATAAGGATACCATTGTAAATGTATTGTCAGCGTGTTCTTCGATTGGGGCATTGAGTTTAGGCCAGTGGGTGAATTCGTATATAAGTGCACGGCCAGATCTCGTGTTGAATGGCATTCTGGGAAATGCATTAATAAACATGTATGTAAAGTATGGGGAAGTAGGTATGGCAGTTTCGGTTTTTAAAACCCTGGTTTGCAAGGATCACATTTCGTGGAGTACCCTCATAAGCGGCATGGCCATGAATGGTTATGGTATACACACGTTGCAGCTTTTTTCGCTCATGCTAGTCCACGGGATTCCTCTTGATGATGTAACCTTCCTTGGCTTGTTATCAGCATGTAGTCACGCATGTCTAGTTGATCAAGGGTTAATGATTTTCAATGCTATTAAAAATGTTTATCGGATTGCCCCTCAAACACTGCACTACGCCTGCCTAGTTGATATGTATGGTCGAGCAGGGTTCTTAGAGGAAGCAGAGAATTTCATGAAGGAAATGCCAACAGAAGTAGACGGCGTTGTTTGGGGAGCGTTGCTTAATGCATGCAAAATTCATGGGAATGAGAAGATACTTAAGAGGATCAGAAATGGTCTCCTCAAGAGTAGAGGAGTAGGCATCGGAACTTATGCTCTCGTATCGAATACATACACCACTCACGAACGGTGGGATGAGGCTAATAAGGTTCGTGATGAAATGAGACAAATGGGGTTGAAGAAATTAGCTGCATACAGTCAGATATATGTGGATCCAAACACATTGACGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

444

Amino Acids

49.34

Weight (kDa)

7.18

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 394 - 427 4.1e-06 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 817, 1071
AccII CGCG 1 cut(s) 105
AciI CCGC 4 cut(s) 103, 314, 348, 792
AclWI GGATC 4 cut(s) 771, 1148, 1301, 1314
AcoI YGGCCR 2 cut(s) 653, 798
AcsI RAATTY 3 cut(s) 634, 1042, 1110
AfaI GTAC 1 cut(s) 781
AflII CTTAAG 1 cut(s) 1133
AflIII ACRYGT 2 cut(s) 699, 822
AgsI TTSAA 3 cut(s) 670, 943, 1273
AjnI CCWGG 1 cut(s) 750
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 5 cut(s) 176, 368, 523, 832, 1283
AluI AGCT 5 cut(s) 176, 368, 523, 832, 1283
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 2 cut(s) 1157, 1252
Alw44I GTGCAC 1 cut(s) 648
AlwI GGATC 4 cut(s) 771, 1148, 1301, 1314
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 4 cut(s) 85, 622, 653, 798
ApaLI GTGCAC 1 cut(s) 648
ApeKI GCWGC 2 cut(s) 829, 1283
ApoI RAATTY 3 cut(s) 634, 1042, 1110
AseI ATTAAT 1 cut(s) 692
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 4 cut(s) 89, 118, 196, 197
AsuHPI GGTGA 1 cut(s) 643
AvaI CYCGRG 1 cut(s) 195
BaeGI GKGCMC 2 cut(s) 123, 652
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BalI TGGCCA 1 cut(s) 800
BamHI GGATCC 1 cut(s) 1306
BauI CACGAG 1 cut(s) 662
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 2 cut(s) 841, 1270
BccI CCATC 1 cut(s) 529
BceAI ACGGC 2 cut(s) 668, 1090
BciT130I CCWGG 1 cut(s) 752
BciVI GTATCC 1 cut(s) 562
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 4 cut(s) 89, 118, 196, 197
BcoDI GTCTC 2 cut(s) 1157, 1252
BfaI CTAG 3 cut(s) 848, 917, 998
BfrI CTTAAG 1 cut(s) 1133
BfuI GTATCC 1 cut(s) 562
BglII AGATCT 1 cut(s) 658
BisI GCNGC 4 cut(s) 314, 793, 830, 1284
BlsI GCNGC 4 cut(s) 315, 794, 831, 1285
Bme1390I CCNGG 5 cut(s) 89, 118, 196, 197, 752
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 1308
BmrFI CCNGG 5 cut(s) 89, 118, 196, 197, 752
BmsI GCATC 1 cut(s) 1185
BpuEI CTTGAG 2 cut(s) 35, 1142
BpuMI CCSGG 4 cut(s) 89, 118, 196, 197
BsaAI YACGTR 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 1157
BsaJI CCNNGG 4 cut(s) 195, 750, 853, 883
BsaXI ACNNNNNCTCC 3 cut(s) 32, 537, 567
Bse1I ACTGG 1 cut(s) 625
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 4 cut(s) 195, 750, 853, 883
BseGI GGATG 3 cut(s) 138, 313, 1234
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 2 cut(s) 1145, 1182
BseSI GKGCMC 2 cut(s) 123, 652
BseXI GCAGC 2 cut(s) 841, 1270
BsgI GTGCAG 1 cut(s) 968
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 87, 624, 655, 800
BsiHKAI GWGCWC 1 cut(s) 652
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 3 cut(s) 88, 117, 196
BsmAI GTCTC 2 cut(s) 1157, 1252
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 4 cut(s) 87, 624, 655, 800
Bso31I GGTCTC 1 cut(s) 1157
BsoBI CYCGRG 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 123, 652
Bsp143I GATC 5 cut(s) 658, 763, 922, 1140, 1306
BspACI CCGC 4 cut(s) 103, 314, 348, 792
BspANI GGCC 4 cut(s) 87, 624, 655, 800
BspFNI CGCG 1 cut(s) 105
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 1 cut(s) 1308
BspPI GGATC 4 cut(s) 771, 1148, 1301, 1314
BspTI CTTAAG 1 cut(s) 1133
BspTNI GGTCTC 1 cut(s) 1157
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 4 cut(s) 195, 750, 853, 883
BssMI GATC 5 cut(s) 658, 763, 922, 1140, 1306
BssNAI GTATAC 1 cut(s) 818
BssSI CACGAG 1 cut(s) 662
BssT1I CCWWGG 1 cut(s) 883
Bst1107I GTATAC 1 cut(s) 818
Bst2BI CACGAG 1 cut(s) 662
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 2 cut(s) 1224, 1292
BstAFI CTTAAG 1 cut(s) 1133
BstBAI YACGTR 1 cut(s) 166
BstC8I GCNNGC 3 cut(s) 103, 994, 1105
BstDEI CTNAG 1 cut(s) 1027
BstDSI CCRYGG 1 cut(s) 853
BstF5I GGATG 3 cut(s) 138, 313, 1234
BstFNI CGCG 1 cut(s) 105
BstKTI GATC 5 cut(s) 661, 766, 925, 1143, 1309
BstMAI GTCTC 2 cut(s) 1157, 1252
BstMBI GATC 5 cut(s) 658, 763, 922, 1140, 1306
BstMWI GCNNNNNNNGC 2 cut(s) 53, 838
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 5 cut(s) 60, 703, 903, 915, 1107
BstSCI CCNGG 5 cut(s) 87, 116, 194, 195, 750
BstSLI GKGCMC 2 cut(s) 123, 652
BstSNI TACGTA 1 cut(s) 166
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 841, 1270
BstX2I RGATCY 2 cut(s) 658, 1306
BstYI RGATCY 2 cut(s) 658, 1306
BstZ17I GTATAC 1 cut(s) 818
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 4 cut(s) 87, 624, 655, 800
BtgI CCRYGG 1 cut(s) 853
BtsCI GGATG 3 cut(s) 138, 313, 1234
BtsI GCAGTG 3 cut(s) 450, 522, 980
BtsIMutI CAGTG 4 cut(s) 450, 522, 632, 980
Cac8I GCNNGC 3 cut(s) 103, 994, 1105
CciI TCATGA 1 cut(s) 1047
Cfr13I GGNCC 1 cut(s) 85
Cfr9I CCCGGG 1 cut(s) 195
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 780
CviQI GTAC 1 cut(s) 780
DdeI CTNAG 1 cut(s) 1027
DpnI GATC 5 cut(s) 660, 765, 924, 1142, 1308
DpnII GATC 5 cut(s) 658, 763, 922, 1140, 1306
DraI TTTAAA 2 cut(s) 372, 745
EaeI YGGCCR 2 cut(s) 653, 798
Eco105I TACGTA 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 883
Eco31I GGTCTC 1 cut(s) 1157
Eco88I CYCGRG 1 cut(s) 195
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 750
EcoT14I CCWWGG 1 cut(s) 883
EcoT22I ATGCAT 2 cut(s) 691, 1105
ErhI CCWWGG 1 cut(s) 883
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 2 cut(s) 817, 1071
Fnu4HI GCNGC 4 cut(s) 314, 793, 830, 1284
FokI GGATG 3 cut(s) 125, 320, 1241
Fsp4HI GCNGC 4 cut(s) 314, 793, 830, 1284
FspBI CTAG 3 cut(s) 848, 917, 998
GluI GCNGC 4 cut(s) 314, 793, 830, 1284
HaeIII GGCC 4 cut(s) 87, 624, 655, 800
HapII CCGG 3 cut(s) 88, 117, 196
HgaI GACGC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 2 cut(s) 206, 859
HpaII CCGG 3 cut(s) 88, 117, 196
HphI GGTGA 1 cut(s) 643
Hpy166II GTNNAC 5 cut(s) 237, 650, 818, 853, 1072
Hpy188I TCNGA 6 cut(s) 130, 418, 966, 1145, 1181, 1296
Hpy188III TCNNGA 6 cut(s) 530, 866, 1048, 1159, 1217, 1247
Hpy8I GTNNAC 5 cut(s) 237, 650, 818, 853, 1072
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 4 cut(s) 46, 145, 889, 1045
HpyCH4III ACNGT 2 cut(s) 1224, 1292
HpyCH4IV ACGT 3 cut(s) 165, 824, 1322
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 838
HpyF3I CTNAG 1 cut(s) 1027
HpySE526I ACGT 3 cut(s) 165, 824, 1322
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 5 cut(s) 658, 763, 922, 1140, 1306
LmnI GCTCC 2 cut(s) 365, 1088
Lsp1109I GCAGC 2 cut(s) 841, 1270
LweI GCATC 1 cut(s) 1185
MaeI CTAG 3 cut(s) 848, 917, 998
MaeII ACGT 3 cut(s) 165, 824, 1322
MaeIII GTNAC 4 cut(s) 166, 506, 874, 905
MalI GATC 5 cut(s) 660, 765, 924, 1142, 1308
MboI GATC 5 cut(s) 658, 763, 922, 1140, 1306
MboII GAAGA 3 cut(s) 591, 1138, 1285
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 2 cut(s) 658, 1306
MhlI GDGCHC 2 cut(s) 123, 652
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 6 cut(s) 285, 319, 634, 1042, 1110, 1277
MluNI TGGCCA 1 cut(s) 800
MmeI TCCRAC 1 cut(s) 429
Mox20I TGGCCA 1 cut(s) 800
Mph1103I ATGCAT 2 cut(s) 691, 1105
MscI TGGCCA 1 cut(s) 800
MseI TTAA 9 cut(s) 258, 291, 371, 692, 744, 932, 951, 1098, 1134
MslI CAYNNNNRTG 4 cut(s) 308, 341, 800, 1119
Msp20I TGGCCA 1 cut(s) 800
MspCI CTTAAG 1 cut(s) 1133
MspI CCGG 3 cut(s) 88, 117, 196
MspR9I CCNGG 5 cut(s) 89, 118, 196, 197, 752
MunI CAATTG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 1 cut(s) 752
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 53, 838
NciI CCSGG 4 cut(s) 89, 118, 196, 197
NdeII GATC 5 cut(s) 658, 763, 922, 1140, 1306
NlaIV GGNNCC 1 cut(s) 1308
NmuCI GTSAC 2 cut(s) 506, 905
NsiI ATGCAT 2 cut(s) 691, 1105
NspI RCATGY 5 cut(s) 60, 703, 903, 915, 1107
PaeI GCATGC 1 cut(s) 1107
PagI TCATGA 1 cut(s) 1047
PciI ACATGT 1 cut(s) 699
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 675
PfeI GAWTC 2 cut(s) 206, 859
PkrI GCNGC 4 cut(s) 315, 794, 831, 1285
Ppu21I YACGTR 1 cut(s) 166
PscI ACATGT 1 cut(s) 699
PshBI ATTAAT 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 750
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 1308
PspPI GGNCC 1 cut(s) 85
PsuI RGATCY 2 cut(s) 658, 1306
RsaI GTAC 1 cut(s) 781
RsaNI GTAC 1 cut(s) 780
RseI CAYNNNNRTG 4 cut(s) 308, 341, 800, 1119
SaqAI TTAA 9 cut(s) 258, 291, 371, 692, 744, 932, 951, 1098, 1134
SatI GCNGC 4 cut(s) 314, 793, 830, 1284
Sau3AI GATC 5 cut(s) 658, 763, 922, 1140, 1306
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 5 cut(s) 89, 118, 196, 197, 752
SduI GDGCHC 2 cut(s) 123, 652
SfaNI GCATC 1 cut(s) 1185
SmaI CCCGGG 1 cut(s) 197
SmiMI CAYNNNNRTG 4 cut(s) 308, 341, 800, 1119
SmlI CTYRAG 3 cut(s) 50, 1133, 1157
SmoI CTYRAG 3 cut(s) 50, 1133, 1157
SnaBI TACGTA 1 cut(s) 166
SphI GCATGC 1 cut(s) 1107
Sse9I AATT 6 cut(s) 285, 319, 634, 1042, 1110, 1277
SsiI CCGC 4 cut(s) 103, 314, 348, 792
SspMI CTAG 3 cut(s) 848, 917, 998
StyD4I CCNGG 5 cut(s) 87, 116, 194, 195, 750
StyI CCWWGG 1 cut(s) 883
TaaI ACNGT 2 cut(s) 1224, 1292
TaiI ACGT 3 cut(s) 168, 827, 1325
TaqI TCGA 6 cut(s) 204, 242, 356, 602, 1015, 1199
TasI AATT 6 cut(s) 285, 319, 634, 1042, 1110, 1277
TauI GCSGC 2 cut(s) 316, 795
TfiI GAWTC 2 cut(s) 206, 859
Tru1I TTAA 9 cut(s) 258, 291, 371, 692, 744, 932, 951, 1098, 1134
Tru9I TTAA 9 cut(s) 258, 291, 371, 692, 744, 932, 951, 1098, 1134
TscAI CASTG 4 cut(s) 450, 522, 632, 987
TseFI GTSAC 2 cut(s) 506, 905
TseI GCWGC 2 cut(s) 829, 1283
Tsp45I GTSAC 2 cut(s) 506, 905
TspDTI ATGAA 7 cut(s) 127, 380, 818, 1036, 1064, 1103, 1266
TspMI CCCGGG 1 cut(s) 195
TspRI CASTG 4 cut(s) 450, 522, 632, 987
Vha464I CTTAAG 1 cut(s) 1133
VneI GTGCAC 1 cut(s) 648
VspI ATTAAT 1 cut(s) 692
XapI RAATTY 3 cut(s) 634, 1042, 1110
XceI RCATGY 5 cut(s) 60, 703, 903, 915, 1107
XmaI CCCGGG 1 cut(s) 195
XmiI GTMKAC 2 cut(s) 817, 1071
XspI CTAG 3 cut(s) 848, 917, 998
Zsp2I ATGCAT 2 cut(s) 691, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.